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Effects of Body Weight Control Methods during Rearing Phase on Laying Performance in Broiler Breeder Pullets (육용종계 육성기 체중조절 방법이 산란기 생산성에 미치는 영향)

  • Na, Jae-Cheon;Lee, Sang-Jin;Jang, Byeong-Gwi;Kim, Sang-Ho;Kim, Hak-Kyu;Suh, Ok-Seok;Lee, Gin-Keon;Yu, Dong-Jo;Ha, Jeong-Gi
    • Korean Journal of Poultry Science
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    • v.30 no.4
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    • pp.229-233
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    • 2003
  • A study was conducted to investigate the effects of different BW control methods during rearing on laying performance of broiler breeder pullets. D-old 540 female breeder chicks (Arbor Acres) were assigned to three treatments consisted of standard BW (Control), 110% of standard BW at 12 wk of age (T1), and 90% of standard BW at 12 wk of age (T2), with three replicates of 60 birds per replicate (pen) for each treatment. At 20 wk of age, all birds from three treatments reached the BW reqired in the Arbor Acres Manual. There were no significant differences in egg production, egg weight and viability during laying period(p>0.05). However, total egg production rates were improved in T2 and T3. Average egg weight was the highest in T1 among all treatments. Fertility and hatchability were similar among treatments, but T2 tended to be higher than other treatments at 37 and 53 wk of age. No significant difference was found in hatchability among three treatments. The number of hatching egg of T2 reached 168 per year, showing higher number of eggs than did the other treatments. The number of hatched chicks in T2 was 131, which was also higher than the other treatments, but the difference was not significant. It appears that the laying performance of broiler breeder hens could be improved when their BW at 12 wk of age are kept at 90% of standard BW, and reach the standard BW at 20 week of age.

The Effectiveness of CT and MRI Contrast Agent for SUV in 18F-FDG PET/CT Scanning (18F-FDG PET/CT 검사에서 정량분석에 관한 CT와 MRI 조영제의 효과)

  • Cha, Sangyoung;Cho, Yonggwi;Lee, Yongki;Song, Jongnam;Choi, Namgil
    • Journal of the Korean Society of Radiology
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    • v.10 no.4
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    • pp.255-261
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    • 2016
  • In this study, among various factors having influence on SUV, we intended to compare and analyze the change of SUV using CT(4 type) and MRI(3 type) contrast agents which are commercialized now. We used Discovery 690 PET/CT(GE) and NEMA NU2 - 1994 PET phantom as experimental equipment. We have conducted a study as follows; first, we filled distilled water to phantom about two-thirds and injected radioisotope(18F-FDG 37 MBq), contrast agent. Second, we mixed CT contrast agent with distilled water and MRI contrast agent with that water separately. And then, we stirred the fluid and filled distilled water fully not to make air bubble. In emission scan, we had 15minutes scanning time after 40 minutes mixing contrast agent with distilled water. In transmission scan, we used CT scanning and its measurement conditions were tube voltage 120 kVp, tube current 40 mA, rotation time 0.5 sec, slice thickness 3.27 mm, DFOV 30 cm. Analyzing results, we set up some ROIs in 10th, 15th, 20th, 25th, 30th slice and measured SUVmean, SUVmax. Consequently, all images mixed 3 types of MRI contrast agent with distilled water have high SUVmean as compared with pure FDG image but there was no statistical significance. In SUVmax, they have high score and there was statistical significance. And other 4 images mixed 4 types of CT contrast agent with distilled water have significance in both SUVmean and SUVmax. Attenuation correction in PET/CT has been executed through various methods to make high quality image. But we figured out that using CT and MRI contrast agents before PET/CT scanning could make distortion of image and decrease diagnostic value. In that reason, we have to sort out the priority of examination in hospital not to disturb other examination's results. Through this process, we will be able to give superior medical service to our customers.

Oocyst Production and immunogenicity of Cryptosporidium baileyi in chickens and mallards (병아리 및 청둥오리에 있어서 닭와포자충의 오오시스트 배설상황과 면역원성)

  • Lee, Jae-Gu;Jang, Byeong-Gwi;Park, Bae-Geun
    • Parasites, Hosts and Diseases
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    • v.33 no.1
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    • pp.45-54
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    • 1995
  • Two-day-old chickens{\;}and{\;}mallards were orally inoculated with one of % doses varying from $2{\;}{\times}{\;}10^2{\;}to{\;}2{\;}{\times}{\;}10^6$ of C. bailevi oocysts per individual. Generally, the more oocysts Inoculated were, the longer the patent periods were, and the more oocysts shedding were. Meanwhile increasing the inoculative dose, the prepatent periods were shortened except that mallards inoculated with $2{\;}{\times}{\;}10^2and{\;}2{\;}{\times}{\;}10^3$ oocysts foiled to produce the oocysts. The more parasites involving oocysts appeared from the chicken in comparison to the mallard. In the chickens challenged with a single dose of $2{\;}{\times}{\;}10^6$ oocysts, a small number of oocysts were detected from feces on days 4-14 after challenge infection (ACI) in all of carrageenan administered groups and in the control groups inoculated with $2{\;}{\times}{\;}10^2{\;}and{\;}2{\;}{\times}{\;}10^3$ oocysts. In the mallards, a few oocysts were also recognized on days 5-15 ACI in all of carrageenan treated groups and in the control groups inoculated with $2{\;}{\times}{\;}10^2,{\;}2{\;}{\times}{\;}10^3{\;}and{\;}2{\;}{\times}{\;}10^4$ oocysts. Just prior to challenge infection, phagocytic activity of peritoneal macrophages (Me) and the number of peripheral Mc in both birds were significantly decreased in the carrageenan treated groups as compared to the control groups. Mild challenge inection in both birds denoted that the immunogenicity of C. bailelli to the birds was very strong, despite MB blocker carrageenan administration.

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Changes in Internal and External Temperature and Microbiological Contamination depending on Consumer Behavior after Purchase of Fresh-Cut Produces (대형마트 신선편의식품 소비자의 구매 후 행동에 따른 식품 내·외부 온도 및 미생물학적 오염 변화)

  • Park, Hyun-Jin;Lee, Jeong-Eun;Kim, Sol-A;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.35 no.5
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    • pp.459-467
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    • 2020
  • In this study, we investigated the changes in both ambient temperature and microbial contamination of fresh convenience foods (FCFs) caused by the behavior of consumers after purchase. According to consumer survey results, it took 0.5 to 3 h put the purchased FCF in a home refrigerator or consume it. Only aerobic bacteria and Staphylococcus aureus (below maximum permitted limit) were detected in FCFs obtained from a local market. During storage of FCFs in a vehicle trunk for up to 3 h. the external and internal temperatures of FCFs were found to be 19 and 18.5℃ in spring, 44 and 42℃ in summer, 31.3 and 29.2℃ in autumn, and 17.6 and 16.8℃ in winter, respectively. Changes in contamination levels of aerobic bacteria on FCFs stored in a vehicle trunk for up to 3 hours are as follows: 2.72 → 3.41 log CFU/g in spring, 3.11 → 4.32 log CFU/g in summer, 3.08 → 3.81 log CFU/g in autumn, 2.71 → 3.36 log CFU/g in winter. S. aureus exceeding the tolerance was detected even when the FCFs were stored in a vehicle trunk for 1 h in summer and autumn and 2 h in spring and winter. Among three boxes (corrugated box, styrofoam box, and corrugated box coated with an aluminum film), the styrofoam box maintained the lowest temperature and showed the lowest growth rate of microorganisms on FCF after storage for 3 h in the vehicle trunk depending on whether ice was added. These results indicated that the possibility of food poisoning occurs when FCFs are exposed to the external environment. It is necessary to provide guidelines regarding storage temperature and allowable time for safe consumption of FCFs after purchase.

Studies on the In Vitro Development of Cloned Embryos by Somatic Cell Nuclear Transfer in Korean Native Goats (재래산양의 체세포 핵이식에 의한 복제수정란의 체외발달에 관한 연구)

  • Park H. S.;Kim T. S.;Jung S. Y.;Lee Y. H.;Jung J. Y.
    • Journal of Embryo Transfer
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    • v.20 no.2
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    • pp.105-112
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    • 2005
  • The present study was conducted to examine some factors affecting in vitro development of oocytes from somatic cell nuclear transfer (SCNT) in Korean native goats. Recipient oocytes were surgically collected after superovulation by using CIDR and FSH, PMSG, hCG and estrous synchronization in Korean Native goats. For nuclear transfer, the fibroblasts from caprine ear cells and fetal fibroblasts were surgically harvested and were cultured in vitro until cell confluency in serum-starvation condition (TCM-199 + $0.5\%$ FBS) for 3 to 5 days. The zona pellucidae of matured oocytes were partially drilled by laser irradiation. A single somatic cell was individually transferred into each enucleated oocyte. The reconstructed oocytes were then electrically fused and activated. Activated NT embryos were cultured in mSOF medium supplemented with $0.8\%\;BSA\;6\~7\;day\;at\;39^{\circ}C,\;5\%\;CO_2,\;5\%\;O_2,\;90\%\;N_2$ in air. There were no significant difference in the number of embryos cleaved and 4-cell development between the fibroblast nuclei from mature ear cells and fetal cells, but the rate of 8-cell development was higher (P<0.05) in ear cells $(40.5\%)$ than in fetal cells $(55.5\%)$. However, the embryo development to morula or blastocyst was not significantly different between both the groups$(6.7\%\;vs\;16.0\%)$, respectively. The number of embryo cleaved $(79.0\%)$ were higher (P<0.05) in the oocytes activated with ionomycin+6-DMAP than in the oocytes activated electrically $(9.5\%)$. The development of fused embryos to morula or blastocyst was found $15.6\%$ in ionomycin+6-DMAP, but no morula or blastocysts were developed in electrical stimulation. The development rate of SCNT embryos to morula or blastocyst was love. (P<0.05) in SCNT embryos $(19.0\%\;vs\;0.0\%)$ than that in parthenotes $(66.1\%\;vs\;59.1\%)$. In the parthenotes, the cleavage rate and development to morula or blastocyst were significantly higher (P<0.05) as $86.8\%\;and\;50.0\%$ in ovulated oocytes than in follicular oocytes $(69.0\%\;vs\;23.6\%)$, respectively. These results suggest that some factors Including superovulation treatment, oocyte source, maturation of follicular oocytes, activation method and culture condition may affect in vitro developmental capability of embryos produced by somatic cell nuclear transfer in Korean Native goats, and the fusion rate be greatly low compared with other species.

A Study on the Recognition and Intake Frequency of Pohang's Local Foods (포항 향토음식의 인지도 및 섭취빈도에 관한 조사연구)

  • Baek, Seo-yeong;Jang, Sun-Ok;Lee, Yeon-Jung
    • Culinary science and hospitality research
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    • v.22 no.5
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    • pp.214-230
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    • 2016
  • This study was carried out to create a momentum for interest and understanding of local foods and provide base line data for the presentation of future effective development direction by investigating and researching cognitive characteristics, intake frequency, and preference about local foods targeting Pohang citizens. As a result of the investigation on intake frequency and preference of Pohang's local foods, in almost every item such as "gwamegi", "mul-hoe", "Guryongpo daege", "sashimi noodles", "mori noodles", "pidegi cuttle fish", "mulgomtang", "homchibap sikhae", "jesabap sikhae", "whale meat", "gaebokchi", "duchichijjim", "octopus sashimi", "roundnose flounder sashimi", "round flounder jorim", "fried round flounder", "gunsu sorasanjeok", "raw mustard muchim", "miyeongui dish", "sigeumjang", "chives dish", "rolled chives egg", "gokgang spinach", "pine dish", "yellow bean leaves muchim", "pickled green bean leaves", "green bean leaves watery kimchi", "Pohang sunrise bread", "black stone eel dish", "chili jjim", "red-pepper leaves namul muchim", "wild greens bibinbap", "acorn jello muchim", "oimiyeok changuk", "dongdongju", etc., significant differences were shown at the significance level of p<0.05~p<0.001. About understandings, views, development prospects, and development value and so forthof Pohang's local foods, more than 80% of respondents were positive, and in the questions asking concerns about local foods, more than half of the respondents gave a positive opinion, citing that they "have a little interest", and "tend to have an interest". Therefore, it can be seen that the future of local foods is bright. In the future, it is required that various cultural events related to local foods are held, a number of promotions are carried out, specialization of stores is distributed, and, at the same time, local foods are developed and generalized to suit modern sense, maintaining traditional taste in order to become global foods, as well as thedeformation of recipes.

Characteristics of marine algae extracts using subcritical water extract method (아임계 추출법을 이용한 해조류 추출물의 특성)

  • Na, Hwan Sik;Kim, Jin Young;Park, Jong Soo;Choi, Gyeong Cheol;Yang, Soo In;Lee, Ji Heon;Cho, Jeong Young;Ma, Seung Jin
    • Food Science and Preservation
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    • v.21 no.1
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    • pp.62-68
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    • 2014
  • This study was performed in order to investigate the functional components of 5 kinds of marine algae. We have collected 5 samples of marine algae, such as the sea mustard (Undaria pinnatifida), sea tangle (Laminaria iaponice), sea weed fusiforme (Hizikia fusiforme), green laver (Entetomotpha), laver (Phophyratenera), which have been harvested in Jeollanam-do. In order to examine the functional effects, 5 kinds of marine algae were extracted with hot water ($80^{\circ}C$, 4 hr), ethanol and methanol (R.T., 4 hr), and subcritical water extract (SWE, 3 MPa, $90^{\circ}C$, $150^{\circ}C$, $210^{\circ}C$). A higher yield of extract was obtained through SWE method (3 MPa, $210^{\circ}C$) in all of the samples obtained. The highest total sugar content was 427.4 mg/g in green laver extracted with SWE (3 MPa, $210^{\circ}C$). The content of the SWE total phenolic compounds was higher than that of the water and solvent (methanol, ethanol) extracts. The anti-oxidative activities of the extracts from 5 kinds of marine algae were examined through the DPPH radical scavenging activity test. The SWE (3 MPa, $150^{\circ}C$ and $210^{\circ}C$) of the marine algae was the highest among all of the extracts. As per the results, the SWE of the marine algae contained more functional components and it had a higher antioxidant activity than those of the other extracts. The $IC_{50}$ value of tyrosinase in seaweed fusiforme and laver were higher than those of the other samples. These results strongly support the possible use of marine algae as functional materials.

Native Tree Species of Tolerance to Saline Soil and Salt Spray Drift at the Coastal Forests in the West-Sea, Korea (한국 서해안의 내염성 및 내조성 자생수종)

  • Kim, Do-Gyun
    • Korean Journal of Environment and Ecology
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    • v.24 no.2
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    • pp.209-221
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    • 2010
  • This study was carried out to apply basic data of the native trees for planting in the salinity area by the vegetation ecological selection. Which focused on native woody species to the tolerances of saline soil and salt spray drift on the coastal forests in the West-Sea, Korea. The soil salinity($EC_{1:5}$) was 0.11dS$m^{-1}$, ranging of 0.00dS$m^{-1}$~0.68dS$m^{-1}$. The soil salinity was gradually decreasing from Belt I to Belt Ⅳ except the Belt I in some coastal windbreaks. The order of decreasing soil salinity was Belt I>Belt II>Belt III>Belt Ⅳ and the soil salinity was $EC_{1:5}$ 0.14dS$m^{-1}$, 0.11dS$m^{-1}$, 0.10dS$m^{-1}$, and 0.08dS$m^{-1}$, respectively. The total 181 taxa consisted of 52 families, 104 genus, 157 species, and 24 varieties were recorded as the trees tolerating to both soil salinity and salt spray drift. The trees emerged in the highest degree of salinity($EC_{1:5}$ 0.51dS$m^{-1}$) was nothing but appearanced Pinus thunbergii Parl., Smilax china L., Quercus dentata Thunb. ex Murray, Quercus serrata Thunb. ex Murray and so on at the level of singular and ideal value. The emerged trees in the high salinity of $EC_{1:5}$0.41dS$m^{-1}$~0.50dS$m^{-1}$ were Albizia kalkora Prain, Melia azedarach L., Paederia scandens (Lour.) Merr. var. scandens These species were trees of tolerance to saline soil. The emerged woody species in all belts were Pinus rigida Mill., Pinus densiflora Siebold & Zucc., Pinus thunbergii Parl., Juniperus rigida Siebold & Zucc. and so on. The woody species with high important value(I.V.) were Pinus densiflora Siebold & Zucc., Pinus thunbergii Parl., Pseudosasa japonica (Siebold & Zucc. ex Steud.) Makino, Smilax china L., Platycarya strobilacea Siebold & Zucc. var. strobilacea for. strobilacea and so on, which can be classified as highly tolerant native trees to salt spray drift.

The Forming Process of the Maisan and Nearby Famous Mountains and the Related Mountain Ranges and Water Systems (마이산과 주변 명산의 형성과정과 그에 관련된 산맥과 수계 변화)

  • Oh, Changwhan;Lee, Seunghwan;Lee, Boyoung
    • The Journal of the Petrological Society of Korea
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    • v.26 no.3
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    • pp.201-219
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    • 2017
  • The Jinan Basin which includes Maisan locates in the central part of the northern boundary of the Yeongnam Massif. The basement rocks of the Jinan Basin and surrounding area are Precambrian gneiss and Mesozoic granite which were exposed on the surface before Cretaceous. The Jinan Basin, one of the Cretaceous pull-apart basins in South Korea, formed along the Yongdong-Gwangju fault system. Maisan is composed of conglomerate deposited in the eastern slope of the Jinan Basin showing the shape of horse ears and the unusual topography where many tafonies were developed. The strike slip fault that caused the Jinan Basin was connected to the deep depth so that the magma formed at 200 km depth could have extruded on the surface causing active volcanic activity in and around the Jinan basin. As a result, Cheonbansan composed of pyroclastic rocks, Gubongsan consisting of volcanic neck and WoonilamBanilam formed by the lava flow, appear around Maisan forming a specific terrain. After the formation of the Jinan Basin and surrounding volcanic rocks, they uplifted to form mountains including Masian; the uplifting time may be ca. 69-38 Ma. At this time, the Noryeong mountain range may be formed in the regions which extended from Chugaryeong through Muju and Jinan to Hampyeong dividing the Geumgang and Seomjingang water systems. Due to the ecological barrier, the Noryeong mountain range, Coreoleuciscus splendidus living in the Geumgang water systems was differentiated from that in the Soemjingang water system. In addition, the Geumgang and Mangyeong-Dongjingang water systems were separated by the Unjangsan, which developed in the NNW direction. As a result, diverse ecosystem have been established in and around Maisan and at the same time, diverse cultural and historical resources related to Maisan's unique petrological features, were also established. Therefore, Maisan and surrounding area can be regarded as a place where a geotourism can be successfully established by combining the ecological, cultural and historical resources with a geological heritage. Therefore Maisan and surrounding areas have a high possibility to be a National Geopark and UNESCO Global Geopark.

Characteristics of $Malassezia$ $pachydermatis$ Isolated from Dogs and Antifungal Effect of Essential Oils (개에서 분리된 $Malassezia$ $pachydermatis$의 특성과 Essential Oil의 항진균 효과)

  • Kim, Joo-Yeon;Olivry, Thierry;Son, Won-Geun
    • Journal of Veterinary Clinics
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    • v.29 no.2
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    • pp.141-147
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    • 2012
  • This work describes the characteristics of $Malassezia$ $pachydermatis$ isolated from dog ear canals and the effect of essential oils on the growth of this organism. Sterile cotton swabs were used to collect specimens from the external ear canal and culture tests were performed to detect the population size of $Malassezia$ yeast. Using three different isolation media, included Sabouraud dextrose agar (SDA) to isolate common $M.$ $pachydermatis$, and SDA supplemented with olive oil (SDAO) and Leeming's medium (LM) to detect lipophilic yeast, $Malassezia$ spp were isolated from 14 of 18 dogs (77.8%); isolation rates were 33.3% in SDA, 72.2% in SDAO and 66.7% in LM media. All $Malassezia$ spp isolates were identified as $M.$ $pachydermatis$ according to results of PCR amplification, but gross colony morphology and SDA growth rates suggested four different subtypes. Large (LC) and medium colony (MC) types respectively describe large colony (diameter > 3 mm) and medium colony (around 2 mm) after 72 hour incubation, and small (SC) type refers to smaller colony (< 1 mm) even after 5 days incubation; lipid dependent colonies did not grow onto SDA. Large Colony type strains were isolated from 4, 11, and 11 samples, MC type strains from 2, 3 and 1 and SC type strains from 1, 2 and 1 in SDA, SDAO and LM, respectively. Lipid-dependent $M.$ $pachydermatis$ (Lipo) were isolated from 3 samples each in SDAO and LM. Anti-$M.$ $pachydermatis$ activity testing was done using disc-diffusion assays and well diffusion tests. Most essential oils inhibited the growth of $M.$ $pachydermatis$ in a range from 0.5% to 1.0% of essential oils. MIC90 and MIC50 were variable depending upon the nature of essential oils. Thyme oil was found to be highly effective in inhibiting the growth of $M.$ $pachydermatis$ in a range from 0.125% to 0.0625% while marjoram and then tea tree oil exhibited lower inhibitory capacity.