• Title/Summary/Keyword: 권태식

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Efficient Use of Lactose for Production of the Soluble Recombinant Human Epidermal Growth Factor in Escherichia coli. (대장균에서 lactose를 이용한 수용성 재조합 인간 상피 세포 성장 인자의 생산)

  • 박세철;권태종;고인영;유광현
    • Microbiology and Biotechnology Letters
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    • v.26 no.1
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    • pp.61-67
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    • 1998
  • Recombinant human epidermal growth factor (rhEGF) was produced by E. coli BL2l (DE3) harboring a plasmid pYHB101. The production of rhEGF was 44.5 mg/L when the E. coli BL2l (pYHB101) was cultured at 27$^{\circ}C$ for 48 hr in the modified MBL medium containing 10 $\mu\textrm{g}$/L glucose with 10 $\mu\textrm{m}$ IPTG/lactose induction at 2 hr after inoculation. It was shown that lactose is able to induce the rhEGF expression of E. coli BL2l (pYHB101) with the same efficiency as IPTG. In the batch culture system, when induced with 10 $\mu\textrm{m}$ lactose, E. coli BL2l (pYHB101) produced maximum 45 mg/L of the rhEGF at 28 hr culture in the modified MBL medium containing 10 g/L glucose. In the semi-fed batch culture system, the volumetric yield was 160 mg/L when the culture was added with 0.5% (w/v) lactose and 0.25% (w/v) yeast extract in the late logarithmic phase and 94.3% of rhEGF was secreted as soluble form. However, when the culture was added with them in the early logarithmic phase, the volumetric yield was 120 mg/L and 20.9% of rhEGF was found in cytoplasmic insoluble aggregates. It was found that the addition time of lactose was important for production of soluble rhEGF from E. coli BL21 (pYHB101).

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On the Numerical Analysis of Korean Food Supply Pattern ($1962{\sim}1969$) (우리나라 식량공급양상(食量供給樣相)의 수량적고찰(數量的考察)($1962{\sim}1969$))

  • Kwon, Tai-Wan;Lee, Yong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.4 no.2
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    • pp.140-150
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    • 1972
  • Using methods of principle component analysis and pattern of analysis. Korean food supply pattern and its change (1962-1969) have been compared whit those of other 40 countries. 1) Among the 4 groups classified on the first and second principle components, Korea belongs to the highest crop grain dependent group of Asia. Although Korea is identified as one of the highest crop consuming countries among the group, a significant increase in the consumption of fruits and vegetables is noticed during the period. 2) According to the pattern of numerics analysis Korean food supply pattern (1969) is similar with those of Japan (1949), philippines, Taiwan, while is quite apart from those of U.S.A., W. Germany, France and Australia. 3) During the period the food supply pattern of Korea has been changed significantly toward the more common locations on the world food supply pattern map. Yet the location is far from the desirable one and its moving direction is characteristic, differing not only those of Europian countries but also Asian neighborhoods. this unique pattern should receive a careful examination and thus proper measures should be undertaken to take care of optimal nation's nutritional requirements.

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Why and Who Participate in Illegal Gambling?: The Psychological Characteristics of Illegal Gamblers (누가, 왜 불법도박을 할까?: 불법도박 경험 수준에 따른 심리적 특성)

  • Junbok Lee;Sangyeon Yoon;Taekyun Hur
    • Korean Journal of Culture and Social Issue
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    • v.20 no.2
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    • pp.155-176
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    • 2014
  • The previous researches of gambling have been rather focused on the legal gambling industry and gambling addiction and ignored the issue of illegal gambling. But, illegal gambling in Korea has been continuously growing in its volume and the numbers of the relevant mental and social problems such as gambling addiction, crimes, suicides, and etc. have been increasing rapidly. The present study investigated the psychological characteristics of illegal gamblers with comparing gamblers who never experienced illegal gambling (NE), who experienced illegal gambling but participate mainly in legal gambling (EIG), and who participate mainly in illegal gambling (MIG). 1317 NEs, 177 EIGs, and 37 MIGs were recruited and completed an online survey that measured individual dispositions (risk-taking tendency, regulatory focus, locus of control), attitudes towards gambling regulations, misconception of illegal gambling, motives (monetary, excitement, socialization), and emotions. First, EIGs and MIGs, compared to NEs, preferred risk-taking, and EIGs were more promotion focused than NEs. Also, EIGs perceived illegal gambling as less illegal and tended to hold more misconceptions about illegal gambling, compared to NEs. Furthermore, EIGs and MIGs had stronger monetary and excitement motivation than NEs. Finally, MIGs were more likely to feel anxious than other groups. Focusing on the illegality of gambling, the characteristics of illegal gamblers are discussed and political implication on illegal gambling is suggested.

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Effect of Permeability Anisotropy on the Effective Radius of Grout Bulb in Horizontal Permeation Grouting - Numerical Study (투수계수 이방성을 고려한 수평 약액 그라우트 구근의 침투 유효 반경에 관한 수치해석적 연구)

  • Baek, Seung-Hun;Joo, Hyun-Woo;Kwon, Tae-Hyuk;Han, Jin-Tae;Lee, Ju-Hyung;Yoo, Wan-Kyu
    • Journal of the Korean Geotechnical Society
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    • v.36 no.11
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    • pp.149-156
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    • 2020
  • Permeation grouting effectively enhances soil strength and decreases permeability of soil; however, the flow of grout is heavily affected by anisotropy of hydraulic conductivity in layers. Therefore, this study investigates the effect of permeability anisotropy on the effective radius of horizontal permeation grout using computational fluid dynamics (CFD). We modeled the horizontal permeation grout flow as a two-phase viscous fluid flow in porous media, and the model incorporated the chemical diffusion and the viscosity variation due to hardening. The numerical simulation reveals that the permeability anisotropy shapes the grout bulb to be elliptic and the dissolution-driven diffusion causes a gradual change in grout pore saturation at the edge of the grout bulb. For the grout pore saturations of 10%, 50% and 90%, the horizontal and vertical radii of grout bulb are estimated when the horizontal-to-vertical permeability ratio varies from 0.01 to 100, and the predictive model equations are suggested. This result contributes to more efficient design of injection strategy in formation layers with permeability anisotropy.

A Study on the Radar Reflectivity-Snowfall Rate Relation for Yeongdong Heavy Snowfall Events (영동 대설사례의 레이더 강설강도 추정 관계식에 관한 연구)

  • Jung, Sueng-Pil;Kwon, Tae-Yong;Park, Jun-Young;Choi, Byoung-Choel
    • Atmosphere
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    • v.26 no.4
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    • pp.509-522
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    • 2016
  • Heavy snowfall events have occurred frequently in the Yeongdong region but understanding of these events have trouble in lack of snowfall observation in this region because it is composed of complex topography like the "Taebaek mountains" and the "East sea". These problems can be solved by quantitative precipitation estimation technique using remote sensing such as radar, satellite, etc. Two radars which are able to cover over Yeondong region were installed at Gangneung (GNG) and Gwangdeoksan (GDK). This study uses radar and water equivalent of snow cover to investigate the characteristics of radar echoes and the $Z_e-R$ relations associated with the 10 Yeongdong heavy snowfall events during the last 5 years (2010~2014). It was found that the heights which the probability of detection (POD) of snow detection by GNG radar is more than 80% are 3,000 m and 1,500 m in convective cloud and stratiform cloud, respectively. The vertical gradient of radar reflectivity is less decreased in convective cloud than stratiform cloud. However, POD by GDK radar are lower than 80% at all layers because the majority of Yeondong observational stations are more than 100 km away from GDK radar site. Furthermore, we examined $Z_e-R$ relation from the 10 events using GNG radar and compared the "a" and "b" obtained from these examinations at Sokcho (SC) and Daegwallyeong (DG). These "a" and "b" are estimated from radar echo at 500 m (SC) and 1,500 m (DG). The values of "a" differ in their stations such as SC and DG are 30~116 and 6~39, respectively. But "b" is 0.4~1.7 irrespective of stations. Moreover, the value of "a" increased with surface air temperature. Therefore, quantitative precipitation estimation in heavy snowfall events by radar echo using fixed "a" and "b" is difficult because these values changed according to those precipitation characteristics.

Vulnerability Assessment for Forest Ecosystem to Climate Change Based on Spatio-temporal Information (시공간 정보기반 산림 생태계의 기후변화 취약성 평가)

  • Byun, Jung-Yeon;Lee, Woo-Kyun;Choi, Sung-Ho;Oh, Su-Hyun;Yoo, Seong-Jin;Kwon, Tae-Sung;Sung, Joo-Han;Woo, Jae-Wook
    • Korean Journal of Remote Sensing
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    • v.28 no.1
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    • pp.159-169
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    • 2012
  • The purpose of this study was to assess the vulnerability of forest ecosystem to climate change in South Korea using socio-environmental indicators and the results of two vegetation models named as Hydrological and Thermal Analogy Group(HyTAG), and MAPSS-Century 1(MC1). The changing frequency and direction of biome types estimated by HyTAG model was used for quantifying sensitivity and adaptive capacity of forest distribution. Similarly, the variation and changing tendency of net primary production and soil carbon storage estimated by MC1 model was used for quantifying sensitivity and adaptive capacity of forest function. As socio-environmental indicators, many statistical data such as financial autonomy rate and the number of forestry officer was prepared. All indicators were standardized, and then calculated using the vulnerability assessment equation. The period of vulnerability assessment was divided into the past(1971-2000) and the future(2021-2050). To understand what policy has a priority to climate change, distribution maps of each indicators was depicted and the vulnerability results were compared among administrative districts. Evident differences could be found in entire study area. These differences were mostly derived from regionalspecific adaptive capacity. The result and methodology of this study would be helpful for the development of decision-making supporting system and policy making in forest management with respect to climate change.

A Study on the Thermal Stability of Long-Term Fuel Storage and Lifetime Estimation of Rubber O-ring in Contacted with Fuel (장기 저장연료의 열안정성 및 연료접촉 고무오링의 수명예측 연구)

  • Chung, K.W.;Hong, J.S.;Kim, Y.W.;Han, J.S.;Jeong, B.H.;Kwon, T.S.;Suh, D.O.;Sung, M.J.;Kwon, Y.I.
    • Tribology and Lubricants
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    • v.34 no.5
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    • pp.197-207
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    • 2018
  • Thermal deterioration of fuel due to long-term storage influences engine performance and causes malfunctions. Fuel stability is usually evaluated via heat resistance and thermal stability during a brief heat shock at high temperature; storage stability in this scenario means that there is very little change in the quality of the fuel during long-term storage. In addition, rubber-based products such as oil seals, O-rings, and rubber hoses can influence the quality of the fuel. When these rubber products are in contact with fuel, they can swell, mechanically weaken, and occasionally crack, thus leaking low molar weight rubber and additives including plasticizer and antioxidant into the fuel to degrade its properties and shorten its useful lifetime. This study determines the thermal stabilities of three kinds of synthetic fuels by evaluating their low temperature kinematic viscosities, chemical composition changes via GC analyses, gross heat of combustion, and color changes. We evaluate the compression set of O-rings by immersing one NBR and two FKM rubber O-rings in the three synthetic fuel samples in airtight containers at variable storage temperatures for six months; from this, we estimate the lifetimes of the O-rings using the Power law model. There were very little changes in the chemical compositions and gross heat of combustion after six months of the experiment. The lifetimes are thus dependent on the materials of the rubber products, and in particular, the FKM O-ring was calculated to have a theoretical lifetime of 200 to 5,700 years. These results indicate that the synthetic fuels maintain their physical properties even after long-term storage at high temperatures, and the FKM O-ring is suitable for long-term sealing of these fuels.

Design of Gun Launched Ramjet Propelled Artillery Shell with Inviscid Flow Assumption (비점성 유동을 가정한 포 발사 램제트 추진탄 설계)

  • Kang, Shinjae;Park, Chul;Jung, Woosuk;Kwon, Taesoo;Park, Juhyeon;Kwon, Sejin
    • Journal of the Korean Society of Propulsion Engineers
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    • v.19 no.4
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    • pp.52-60
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    • 2015
  • Operation area of corps was expanded under military reformation, and extending range of 155 mm howitzer became important issue. New approach is needed to extend range to 80 kim. Ramjet engine is air breathing engine, and it can provide specific impulse several times more than solid rocket motor so that range is extended using same weight of propellant. If the ramjet engine is gun-launched system, it does not require any other booster because muzzle velocity is near Mach 3. Especially solid fuel ramjet (SFRJ) does not have any moving part so that it is favorable for gun-launching system which is under high stress during launching. In this paper, we design air intake, combustion chamber, and nozzle of 155 mm gun launched ramjet propelled artillery shell with inviscid flow assumption. We conduct parameter study to have range more than 80 km, and maximum high explosive volume.

Geotechnical investigation on causes and mitigation of ground subsidence during underground structure construction (터널 및 지중매설물 시공에 따른 지반함몰 발생 원인 및 대책에 대한 지반공학적 조사 연구)

  • Choi, Shin-Kyu;Back, Seung-Hun;An, Jun-Beom;Kwon, Tae-Hyuk
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.18 no.2
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    • pp.143-154
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    • 2016
  • This study investigated the occurrences, causes, and mitigation of the recent ground subsidence and underground cavity generation events in Korea. Two main causes of ground subsidence are (1) the soil erosion by seepage during tunneling and earth excavation and (2) the damage of underground pipes. The main cause of the soil erosion during tunneling was the uncontrolled groundwater flow. Especially, when excavating soft grounds using a tunnel boring machine (TBM), the ground near TBM operation halt points were found to be the most vulnerable to failure. The damage of underground pipes was mainly caused by poor construction, material deterioration, and differential settlement in soft soils. The ground subsidence during tunneling and earth excavation can be managed by monitoring the outflow of groundwater and eroded soils in construction sites. It is expected that the ground subsidence by the underground pipe damage can be managed or mitigated by life cycle analysis and maintenance of the buried pipes, and by controlling the earth pressure distribution or increasing the bearing capacity at the upper ground of the buried pipes.

Estimation of surface visibility using MODIS AOD (MODIS AOD를 이용한 지상 시정 산출)

  • Park, Jun-Young;Kwon, Tae-Yong;Lee, Jae-Yong
    • Korean Journal of Remote Sensing
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    • v.33 no.2
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    • pp.171-187
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    • 2017
  • Thisstudy presentsthe method for deriving surface visibility from satellite retrieved AOD. To do thisthe height of aerosol distribution isrequired. This distribution would be in thisstudy represented by the two heights; if there is a discrete atmospheric layer, which is physically separated from the above layer, the upper height of the layer is assumed as Aerosol Layer Height(ALH). In this case there is clear minimum in the Relative Humanity vertical distribution. Otherwise PBLH(Planetary Boundary Layer Height) is used. These heights are obtained from the forecast data of Regional Data Assimilation and Prediction System(RDAPS). The surface visibility is estimated from MODIS AOD and ALH/PBLH, using Koschmieder's Law for ALH and the empirical relations for PBLH. The estimated visibility are evaluated from the visibility measurements of 9 eve-measurement stations and 17 PWD22 stations for the spring of 2015 and 2016. Verification of the estimated visibility shows that there are considerable differencesin statistical verification value depending on stations, years, morning(Terra)/afternoon(Aqua). The better results are shown in the midwest part of korean peninsula for Terra of 2016. The results are summarized as; correlation coefficients of higher than 0.65, for low visibility RMSE of 3.62 km and ME of 2.29 km or less, POD of higher than 0.65 and FAR of 0.5 or less. Verification results were better with increase in the number of low-visibility data.