• The korean journal of orthodontics
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• v.27 no.5 s.64
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• pp.683-696
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• 1997

Recently several growth factors such as $TGF-{\beta}1$, $TGF-{\beta}1$, PDGF, bFGF are known to play an important role in scar formation following adult tissue injury. But there is little known about the role of growth factors in fetal tissue healing without scar formation. Therefore the purpose of this study is to investigate the distribution of growth factors which we involved with scar formation in the artificially created cleft lip wound healing of fetuses. The author had undergone hysterotomy and created cleft lip-like defects on fetuses of New Zealand White Rabbit in mid-third trimester (24 days). Fetuses were divided into 3 groups (the repaired group, the unrepaired group and the sham-operated control group). At 1, 2, 3, 5, 7 days after procedure, the repaired, the unrepaired and the control groups were obtained by Caeserean section. After documenting the viability of fetuses, fetuses were photographed to compare size and facial morphology and sectioned for histological examination by H & E stain and spatial and temporal deposition of $TGF-{\beta}1$, $TGF-{\beta}2$, PDGF, bFGF by immunohistochemical method. The findings are summarized as follows 1. There were lack of inflammation and scar formation and neovascularity in the repaired and the unrepaired group during experimental periods. 2. The reepithelialization of the unrepaired group was slower than that of repaired group. 3. There were no differences of distribution of bFGF in the control, the repaired and the unrepaired group. 4. PDGF was increased at post-op. first and second day and decreased after post-op. third day. Eventually, there were no differences in the control, the repaired and the unrepaired group. 5. $TGF-{\beta}1$ and $TGF-{\beta}2$ were slightly increased at post-op. first and second day and decreased after post-op. third day. Eventually there were no differences in the control, the repaired and the unrepaired group. And $TGF-{\beta}2$ is more densely stained than $TGF-{\beta}1$.

• The korean journal of orthodontics
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• v.34 no.3 s.104
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• pp.261-267
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• 2004

Nonsyndromic cleft lip and/or palate (NSCLP) is one of the most common congenital deformities and its prevalence in Far East Asia, such as within Korean and Japanese populations, is relatively high. However, in the eastern part of Europe, clefts are relatively rare situations. These ethnic differences infer a genetic background of the disease. The objective of this study was to compare the frequency of single nucleotide polymorphism (SNP) in $TGF-{\beta}3$ between Korean and Romanian cleft families. Korean cleft families samples were collected from twenty-six families (n=78) and Romanian cleft families samples were collected from eighteen families (n=41). For sequencing, the blood or saliva of the subjects was sampled. A single nucleotide polymorphism was observed in the intron 5 of $TGF-{\beta}3$ (Al8141G). The frequency of each allele was significantly different between the Korean and Romanian samples. The Ah allele was present in 18 out of 78 Korean samples (23.1%) and in 27 out of 41 Romanian samples (65.9%). The AG was present in 27 (34.6%) out of 78 Koreans and in 13 (31.7%) out of 41 Romanians. The GG was found in 33 (42.3%) Koreans and in 1 (2.4%) Romanian. The difference between the groups was significant (p<0.001). In conclusion, the frequency of observed SNP was significantly different between the two countries. SNP in $TGF-{\beta}3$ in the Korean population seemed to have a higher possibility of occurrence for nonsyndromic cleft palate than the Romanian population.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.49-58
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• 1992

The purpose of the experiment was to determine the effect of plant density and pattern on growth characteristics, total dry matter yield. protein yield and palatability of sorghum-sudangrass hybrid (Sordan 79). The main treatment was three levels of plant density (Skg, 15kg. 30kg/ha) and the sub treatment was two plant patterns of square and rectangular planting.The experiment was arranged as a split plot design and conducted on the Experimental Livestock Farm of Kon-Kuk Univ. in Chungju, 1989. The results obtained are summarized as follows :1. As the plant density was increased, plant length, leaf length, leaf width(P<0.05) and leaf number were gradually decreased, but in the same plant density, plant length, leaf length, leaf width and leaf number were generally increased in the rectangular plot. 2. In the first cutting time, the ratio of leaf was the highest at the rectangular plot of high density (30kg/ha, 25cm$\times$4cm), but in the second cutting time, the ratio of leaf was the highest at the square plot of medium density (15kg/ha, 14cm$\times$ 14cm). 3. The ratio of dead stubble after cutting was generally increased by increasing the plant density. Also, in the same planting density, the ratio of dead stubble was generally increased in the square plot. 4. The tiller number per plant tended to decreased by increasing thc plant density, and the tiller number was increased in the rectangular plot. In the same plant density. 5. Total fresh and dry matter yield were the highest at the rectangular plot of medium density (IS kg/ha, 33cm$\times$6cm) wholly, but the low density was shown the lowest.6. The crude protein content was increased by increasing the plant density per unit area(P<0.05) and the protein yield was also increased by increasing the planting density. 7. According to the increase of plant density, the stem was thinned, but NDF, ADF did not show different. The palatability was in the order of high (30kg/ha))medium (15kg/ha))low density(5kgiha) at the first cutting time, but it was in the order of high)low)medium density at the second cutting time.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.19.2-19.2
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• 1992

The purpose of the experiment was to determine the effect of plant density and pattern on growthcharacteristics, total dry matter yield. protein yield and palatability of sorghum-sudangrass hybrid(Sordan 79). The main treatment was three levels of plant density (Skg, 15kg. 30kg/ha) and the subtreatment was two plant patterns of square and rectangular planting.The experiment was arranged as a split plot design and conducted on the Experimental Livestock Farm of Kon-Kuk Univ. in Chungju, 1989.The results obtained are summarized as follows :1. As the plant density was increased, plant length, leaf length, leaf width(P<0.05) and leaf number were gradually decreased, but in the same plant density, plant length, leaf length, leaf width and leaf number were generally increased in the rectangular plot.2. In the first cutting time, the ratio of leaf was the highest at the rectangular plot of high density(30kg/ha, 25cm$\times$4cm), but in the second cutting time, the ratio of leaf was the highest at the square plot of medium density (15kg/ha, 14cm$\times$ 14cm). 3. The ratio of dead stubble after cutting was generally increased by increasing the plant density. Also, in the same planting density, the ratio of dead stubble was generally increased in the square plot. 4. The tiller number per plant tended to decreased by increasing thc plant density, and the tiller number was increased in the rectangular plot. In the same plant density. 5. Total fresh and dry matter yield were the highest at the rectangular plot of medium density (ISkg/ha, 33cm$\times$6cm) wholly, but the low density was shown the lowest.6. The crude protein content was increased by increasing the plant density per unit area(P<0.05) and the protein yield was also increased by increasing the planting density. 7. According to the increase of plant density, the stem was thinned, but NDF, ADF did not show different. The palatability was in the order of high (30kg/ha))medium (15kg/ha))low density(5kgiha)at the first cutting time, but it was in the order of high)low)medium density at the second cutting time.

• The korean journal of orthodontics
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• v.30 no.2 s.79
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• pp.197-204
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• 2000

[$K^+$]-selective ion channels were studied in excised inside-out membrane patches from human osteoblast-like cells (G292). Three classes of $K^+$channels were present and could be distinguished on the basis of conductance. Conductances were $270\pm27\;pS,\;113\pm12\;pS,\;48\pm8\;pS$ according to their approximate conductances in symmetrical 140 mM KCl saline at holding potential of -80 mV It was found that the small conductance (48 pS) $K^+$channel activation was dependent on membrane voltage. In current-voltage relationship, small conductance $K^+$channel showed outward rectification, and it was activated by the positive potential inside the membrane. In recordings, single channel currents were activayed by a negative pressure outside the membrane. The membrane pressure increased $P_{open}$ of the $K^+$ channel in a pressure-dependent manner. In the excised-patch clamp recordings, G292 osteoblast-like cells have been shown to contain three types of $K^+$ channels. Only the small conductance (48 pS) $K^+$channel is sensitive to the membrane stretch. These findings suggest that a hyperpolarizing current, mediated in part by this channel, may be associated with early events during the mechanical loading of the osteoblast. In G292 osteoblast-like cells, $K^+$channel is sensitive to membrane tension, and may represent a unique adaptation of the bone cell membrane to mechanical stress.

• Korean Journal of Microbiology
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• v.43 no.4
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• pp.298-303
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• 2007

Antithrombotic activities of water extract of cheongkookjang and cheongkookjang fermented with green tea or mugwort were evaluated on some antithrombosis related activities in vitro and thrombotic death inhibition in vivo. Cheongkookjang made of white soybean (Glycine max) or black small soybean (Rhynchosia nulubilis) showed potent antioxidative activities. Addition of green tea or mugwort during cheongkookjang fermentation increased the antioxidative activity, cheongkookjang with green tea showed more drastic increase compared with cheongkookjang with mugwort. Nitrite scavenging effects of the cheongkookjang extracts were prominent but the addition of green tea or mugwort seldom increased the scavenging effects. All the cheongkookjang extracts showed strong inhibitory activities on platelet aggregation. The inhibitory activities of cheongkookjang were increased considerably by addition of green tea or mugwort even with low concentration. Plasmin unit as fibrinolytic activity was not affected considerably by addition of green tea. Addition of mugwort decreased the activity transiently at low concentration ($0.3{\sim}1.0%$) but increased again slowly at higher concentration ($1{\sim}3%$). In vitro thrombotic death inhibition test, the antithrombotic activity of cheongkookjang made of black small bean with green tea was higher by about 1.5 times compared to that without green tea. As results, cheongkookjang might inhibit antithrombosis not only by fibrinolytic action but also by inhibition of platelet aggregation and antioxidative action. The addition of functional materials such as green tea or mugwort could increase the antithrombotic function, even at low concentration.

• Korean Journal of Cleft Lip And Palate
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• v.3 no.1
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• pp.23-31
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• 2000

Objective: When an alveolar cleft is too large to close with adjacent mucobuccal flaps or large secondary fistula following a primary bilateral palatoplasty exists, a one-stage procedure for bone grafting becomes challenging. In such a case, we used the tongue flap to repair the fistula and cleft alveolus in the first stage, and bone grafting to the cleft defect was performed in the second stage several months later. The purpose of this paper is to report our experiences with the use of an anteriorly-based Y-shaped tongue flap to fit the palatal and labial alveolar defects and the ultimate result of the bone graft. Patients: A series of 14 patients underwent surgery of this type from January 1994 to December 1998.The average age of the patients was 15.8 years old (range: 5 to 28 years old). The mean period of follow-up following the 2nd stage bone raft operation was 45.9 months (range: 9 to 68 months). In nine of the 14 cases, the long-fork type of a Yshaped tongue flap was used for extended coverage of the labial side alveolar defects with the palatal fistula in the remaining cases the short-forked design was used. Results: All cases demonstrated a good clinical result after the initial repair of cleft alveolus and palatal fistula. There was no fistula recurrence, although Partial necrosis of distal margin in long-forked tongue flap was occurred in one case. Furthermore, the bone graft, which was performed an average of 8 months after the tongue flap repair, was always successful. Occasionally, the transferred tongue tissue was bulging and interfering with the hygienic care of nearby teeth; however, these problems were able to be solved with proper contour-pasty performed afterwards. No donor site complications such as sensory disturbance, change in taste, limitations in tongue movement, normal speech impairments or tongue disfigurement were encountered. Conclusion: This two-stage reconstruction of a bilateral cleft alveolus using a Y-shaped tongue flap and iliac bone graft was very successful. It may be indicated for a bilateral cleft alveolus patient where the direct closure of the cleft defect with adjacent tissue or the buccal flap is not easy due to scarred fibrotic mucosa and/or accompanied residual palatal fistula.

• Pediatric Infection and Vaccine
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• v.7 no.2
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• pp.233-239
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• 2000

Purpose : A great deal of youngsters suffer from otitis media, for which antimicrobials are frequently prescribed. Increased antimicrobial resistance forces physicians to judiciously use antimicrobial agents in treating patients with acute otitis media. There have however been few references with regard to otitis media in Korean children, and authors proceeded investigation to look for the causative agents of otitis media in Korean children and their antimicrobial susceptibility. Methods : The study included 65 patients younger than 15 years old who had been cared at the department of pediatrics and otolaryngology in Hanyang University Hospital from July 1994 to June 1999, and diagnosed of otitis media with otorrhea which contained microorganisms isolated in otorrhea culture. The medical records were reviewed for demographic data, isolated organisms and their antimicrobial susceptibility. Results : Among 65 patients, 37(57%) were boys and 28(43%) girls. Distribution of the patients was reciprocal to the age of the patients; 27 patients(41.5%) were younger than 1 year old, 24(36.9%) were 1 to 3 years old with the average of 2.9 years of age. Staphylococus aureus was isolated in 32 patients(49.2%), Streptococcus pneumoniae in 19 patients(29.2%) Haemophilus influenzae in 9 patients(13.8%), Streptococcus oralis in 3 patients(4.6%), Moraxella catarrhalis in 1 patient(1.5%). The isolated microorganisms were not different whether patients had cleft lip/palate or not. The antibiotic resistance rates of S. aureus were ${\geq}90%$ to erythromycin, imipenem, cephalothin, and clindamycin, 86.2% to oxacillin, 25% to chloramphenicol, 12.5% to trimethoprim/sulfamethoxazole(TMP/SMX), and 0% to vancomycin and teicoplanin. The antibiotic resistance rates of S. pneumoniae were 71.4% to penicillin and greater than 60% to erythromycin, tetracycline, TMP/SMX, 7.1% to chloramphenicol, and 0% to vancomycin and teicoplanin. The antibiotic resistance rates of H. influenzae were 55% to ampicillin and TMP/SMX, and 0% to chloramphenicol, ceftriaxone, aztreonam, imipenem and ciprofloxacin. Conclusion : With otorrhea culture, the causative organisms of otitis media appear to be S. aureus, S. pneumoniae and H. influenzae. The high antibiotic resistance rates of the isolated organisms should affect the choice of antibiotics in treating patients with otitis media. Prospective investigations utilizing tympanocentesis in microbiologic studies are needed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.1
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• pp.38-43
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• 1986

This field experiment was conducted to determine the effects of shading degrees (0 : full sunlight, 25, 50 and 75%) on the early growth characteristics and winter survival of grasses. For the test different artificial shading houses were established and pasture species used were orchardgrass, timothy and perennial ryegrass. The experiment was performed at Livestock Experiment Station in Suweon, and sowing date was Aug. 25, 1984. The results are summarized as follows : 1. Generally, root length, new root length, number of new root and tillers of grasses were increased at 0% (full sunlight) of shading, followed by 25, 50 and 75% of shading degrees, in that order. Plant length and leaf elongation, however were increased at 50% of shading, followed by 75, 25 and 0%. 2. The percentage of winter killing of grasses was significantly (P<0.05) increased as the shading degrees increased. Also the percentages of winter killing were 10.1% in orchardgrass, 9.5% in timothy and 14.2% in perennial ryegrass, respectively. 3. A significant negative correlations were observed in the relationship between winter killing and tillers, new root length and number of new root of grasses. However, there were positive correlations between winter killing and plant length or leaf elongation under shading conditions. 4. As the shading degrees are increased by 0, 25, 50 and 75%, the NAR of grasses was tended to be decrease to 10.98, 11.52, 9.81 and 6.12 g/$m^2$/day, in that order. Also there was a negative correlation (r=-0.5943*) between winter killing and NAR of grasses, and NAR of orchardgrass, timothy and perennial ryegrass were 8.58, 8.53 and 11.71 g/$m^2$/day, respectively.

• The korean journal of orthodontics
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• v.32 no.5 s.94
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• pp.327-342
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• 2002

Soft tissue profile is a critical area of interest in the development of an orthodontic treatment and diagnosis. The purpose of this study was to determine the facial profile preference of diversified group and to investigate the relationship between most Preferred facial Profile and existing soft tissue reference lines. A survey instrument of constructed facial silhouettes was evaluated by 894 lay person. The silhouettes had varied nose, lips, chin and soft tissue subnasale point. Seven sets of facial type were computer-generated by an orthodontist to represent distinct facial types. The varied facial profiles were graded on the basis of most preferred to least preferred. Every facial profile were measured by soft tissue reference lines(Ricketts E-line, Burstone B-line) to observe the most preferred facial profile. The results as follows: 1. In reliability test, the childhood group showed lower value than other groups, which means that this group has no concern on facial profile preference. 2. It appears that sexual and age difference made no significant difference in selecting the profile 3. An agreement to least preferred facial profile was higher than an agreement to most preferred facial profile. 4. Coefficient of concordance (Kendall W) was higher in the twentieth group. It means that a profile preference of the twentieth is distinct. 5. A lip protrusion (to Ricketts E-line and Burstone B-line) of most preferred facial profile was similar to measurements of previous study that investigate skeletal and soft tissue of esthetic facial profile of young Korean. So these reference lines can be used valuably in clinics. 6. Profile of excessive lip protrusion or retrusion to E-line & B-line was least preferred. 7. Most preferred profile of all respondents group was straight profile. Profile that showing convex profile was not pre(erred and the least preferred profile was concave profile.