• Title/Summary/Keyword: 구상체

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Formation of Spheroids of Adult Rat Primary Hepatocytes in Polyurethane Foam (폴리우레탄 폼을 이용한 쥐 일차 간세포의 구상체 배양)

  • 안재일;이두훈
    • Journal of Biomedical Engineering Research
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    • v.19 no.3
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    • pp.215-224
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    • 1998
  • This paper is fundamental study to develope the extracorporeal liver support system for patient with fulminant hepatic failure(FHF) or being expected for orthotopic liver transplantation. The polyurethane foam, which is composed of the density of 33kg/m3, the average pore diameter of 500${\mu}{\textrm}{m}$, the closed window of 60-70%, was manufactured with the prepolymer of 15% NCO-, Hepatocytes were inoculated to form spheroids in polyurethane foam. The time of spheroid formation in BSA(Bovine Serum Albumin) coated polyurethane foam was shorter than that in raw polyurethane foam. To verify the function of hepatocyte spheroids, we measured ammonia removal rate, urea and albumin secretion rate. Polyurethane foam was suitable for culture of hepatocyte spheroids. And culture of hepatocyte spheroids in polyurethane foam has high possibility in using as an extracorporeal liver support system.

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A palynological study of the subfamily Asclepiadoideae (Apocynaceae s.l.) in Korea (한국산 박주가리아과(광의의 협죽도과)의 화분학적 연구)

  • Nam, Bo Mi;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.45 no.2
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    • pp.183-191
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    • 2015
  • The palynological characters of 14 taxa of 4 genera (Cynanchum, Metaplexis, Tylophora, Marsdenia) in Asclepiadoideae were observed and measured using SM and SEM. The orientation and granulate surface patterns of the pollinia were important characters for delimitating the genera. The position of the attachment of the translator arms to the pollinia, considered to be an important character for recognizing the above genera, was an overlapping character among the genera and can therefore be utilized only as taxonomy at the species level. Also, we found for the first time that only Tylophora floribunda has an incised margin of the corpusculum. The quantitative characters of the pollinia, arms and corpusculum were useful keys for the delimitation of the species.

Dual-porosity fractal model with parallel fracture and blocky fracture flow (판상체 및 입방체 이중공극 프랙탈 모델의 지하수위 거동)

  • 함세영
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1998.11a
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    • pp.127-130
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    • 1998
  • 이 연구에서는 부정류의 판상체 이중공극 프랙탈 모델과 입방체 이중공극 프랙탈 모델의 지하수위 거동을 비교 연구하였다. 균열내 지하수위 거동 해석은 판상 이중공극 프랙탈 모델은 Hmm과 Bidaux(1996)을 이용하였고 입방체 이중공극 프랙탈 모델의 경우에는 입방체블록과 같은 크기의 구상체 블록으로 간주하여 지하수위 거동을 해석하였다. 그리고 0.5, 1, 1.5, 2, 2.5, 3차원에 대해서 판상체 이중공극 프랙탈 모델과 입방체 이중공극 프랙탈 모델의 이론적인 수위강하 곡선을 작성하여 비교, 분석하였다. 부정류의 판상체 이중공극 프랙탈 모델과 입방체 이중공극 프랙탈 모델은 기반암내 균열의 분포가 프랙탈망을 형성하고, 균열과 매트릭스 블록이 거의 수평의 층상으로 발달하는 경우와 균열이 수평방향과 수직방향으로 발달하면서 매트릭스 블록이 입방체를 이루는 경우에 적용될 수 있다.

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The Origin and Age of the Orbicular Granite Gneiss in Wangjungri, Muju (무주 왕정리 일대 구상 화강편마암의 성인과 형성시기)

  • Oh, Chang Whan;Lee, Byung Choon;Yi, Keewook
    • The Journal of the Petrological Society of Korea
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    • v.22 no.2
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    • pp.117-135
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    • 2013
  • Orbicular granite gneisses occur as a xenolith within two-mica leucogranites, together with early Paleoproterozoic metasedimentary xenoliths, in Wangjeong-ri, Muju area. The whole-rock chemistries and SHRIMP zircon Pb/U ages of the leucogranites indicate that they are S-type granitoids formed in the continental tectonic setting at $1875{\pm}75$ Ma. The SHRIMP age of monazites from the orbicular granite gneiss gives $1867{\pm}4$ Ma as a metamorphic age which is similar to the intrusion age of the two-mica leucogranite within the error range. The similar ages between zircons and monazites represent that the orbicular granite gneisses formed by metamorphism during the intrusion of the two-mica leucogranite; the metasedimetary xenoliths which sank within the parent magma of leucogranites were metamorphosed into orbicular granite gneisses by thermal metamorphism ($650-740^{\circ}C$, 4-6.5 kbar) due to the heat supplied from surrounding magma. During the thermal metamorphism, the core of orbicular granite gneiss mainly consisting of cordierite formed, and in some orbicular granitic gneisses, the leucocratic melt formed by melting of quartz and plagioclase in the core, squeezed out from core and crystallized around the core forming outer rim. The hydrothermal fluid at the late stage of magma differentiation penetrated into the orbicular granite gneisses resulting pinitization of cordierite into chlorite and sericite. As Muju orbicula granite gneiss was formed from sedimentary rocks, it is more appropriate to be called Muju orbicula granitic gneiss.

Changes of Protein Bodies in Endosperm Cells during Embryo Development of Ginseng (Panax ginseng C.A. Meyer) Seeds - Seeds with Red Seed Coat and Indehiscent Seeds - (인삼(Panax ginseng C.A. Meyer) 종자의 배발달에 따른 배유세포의 단백과립 변화 - 홍숙 및 미개갑 종자 -)

  • 유성철
    • Journal of Plant Biology
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    • v.35 no.1
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    • pp.45-51
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    • 1992
  • The changes of protein bodies in endosperm cells of both seeds with red seed coat and indehiscent seeds of Panax ginseng C.A. Meyer have been investigated in relation to the embryo development. In the early stage of seeds with red seed coat, spherical spherosomes were distributed in endosperm cells. Protein bodies were formed from vacuoles containing the storage protein. Cell organelles were hardly observed in the cytoplasm. In the late stage of the seed with red seed coat, the endosperm was filled with spherosomes and protein bodies. The protein bodies consisted of amorphous inclusions with high electron density or proteinaceous matrix with even electron density. In the seed of in dehiscence, the protein body in endosperm cells contained globoids and protein crystalloids. The globoid of protein body had a electron dense materials. Umbiliform layer was formed between embryo and endosperm. The deformation patterns of endosperm cell wall and the cellulose microfibril were observed in endosperm cells near the umbiliform layer. Umbiliform layer consisted of lipid body and autolyzed cell debris. The protein body of endosperm cell near the umbiliform layer showed various degenerative patterns, and so electron density of proteinaceous matrix was gradually decreased.reased.

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