• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.3
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• pp.226-235
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• 1991

This experiment was carried out to obtain the information on the crossability, germination rate, and pollen viability of the progeny from the cross between hexaploid triticale cv. Sinkihomil and five hexaploid wheat varieties. The results are summarized as follows. Seed set was 28.8 to 41.8% (ave-raged 34.1%) in the cross between triticale and wheat, which resulted in 3.61% in F$_2$(selfed F$_1$), 3.99% in F$_1$/P$_1$ 21. 9% in F$_1$/P$_2$ respectively. However, seed set was extremely low in reciprocal crosses when triticale was used as male. In the backcross, crossability was higher in F$_1$/wheat and triticale/F$_1$ than that in wheat/F$_1$ and F$_1$/triticale, respectively. Germination rate of the crossed seed was 95% in F$_1$ 66.3% in F$_2$ (selfed F$_1$), 62.0% in F$_1$/P$_1$ and 81.0% in F$_1$/P$_2$ from the cross between triticale and wheat, respectively. When triticale cv. Sinkihomil was used as male, seeds were degenerated because of the failure of endorsperm development. This might be caused by AAABBBDDR genome constitution in the cytoplasm from a hexaploid wheat. Pollen fertility of F, plant was averaged 34.1% in the cross between triticale and wheat. Significant positive correlation between the pollen fertility and seed set rate in the cross between triticale and wheat were detected.

• The Korean Journal of Mycology
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• v.25 no.1 s.80
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• pp.20-25
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• 1997

In comparison of three Pleurotus species and their selfed and crossed isolates using SDS-polyacrylamide gel electrophoresis of total soluble proteins, Pleurotus ostreatus 201 showed low similarity to selfed or P. ostreatus 201 crossed ones. Pleurotus ostreatus 2042 showed low similarity to selfed or P. ostreatus 2042 crossed ones. However, P. ostreatus $2042{\times}P$. ostreatus 202, P. ostreatus $2042{\times}P$. sajor-caju, and P. ostreatus $2042{\times}P$. ostreatus 900 showed high similarity. Pleurotus ostreatus 202 showed low similarity to selfed or crossed ones. Pleurotus sajorcaju showed low similarity to selfed or crossed ones. Pleurotus ostreatus 900 showed low similarity to selfed or crossed ones. However, selfed P. ostreatus and P. $ostreatus{\times}P$. florida showed high similarity. Pleurotus florida and selfed P. florida showed high similarity, too.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.612-616
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• 2006

In recent years, there has been increasing concerns in gene flow from GM crops to wild or weedy relatives as a potential risk in the commercialization of GM crops. To access the possibility of the environmental impacts by GM rice, small-scale experiments of gene transfer were carried out. Herbicide and drought stress resistant GM rice and non-GM rice Nakdongbyeo, wild rice Oryza nivara, and weedy rice Sharebyeo were used for artificial pollination experiments and bar gene was used as a tractable marker after pollination. The harvested putative hybrid seeds after artificial pollination were germinated and true hybrid plants were selected by basta treatment. The hybrid plants were verified again by PCR amplification of bar and trehalose-6-phosphate phosphatase (TPP) genes and RAPD PCR analysis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.640-649
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• 1996

This study was conducted to obtain fundamental informations necessary to breed forage sorghum at the field of the Livestock Experiment Station from 1988 to 1991. Heterosis was discussed in crossing groups of sorghum X sweet sorghum, sorghum X sudangrass, and (sorghum X sweet sorghum) X sudangrass. Leaf dry weight and stalk dry weight per plant in sweet sorghum crossing group, stalk dry weight per plant in sorghum X sudangrass crosses and leaf area per plant in three way crosses showed the greatest Heterobeltiosis(H$_{b}$). There were significant differences in plant height, stalk diameter and number of tillers per plant between sweet sorghum and sudangrass crosses. H$_{b}$ for total dry matter yield in sweet sorghum and sudangrass crosses were 45.9% and 95.0%, respectively. On the other hand, heterosis for total dry matter yield in three way crosses was smaller than H$_{b}$. There was no relationship between dry matter yield of parents and heterosis of hybrids in sweetsorghum crosses. However, positive correlations between parental yield and hybrid yields were observed. In sudangrass crosses, there were negative correlations between parental yields and heterosis of hybrids. However, no correlation between parental yields and hybrid yields were observed. In three way crosses, there were no correlations between parental yields and heterosis, and between parental yields and hybrids yields.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.35-43
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• 2015

To assess the dormancy associated weedy risk of the F1 hybrid generated by hybridization between Brassica juncea (maternal) and Brassica napus (paternal), seed germination, dormancy and longevity were examined sequentially after seed harvest. The F1 hybrids exhibited the intermediate characteristics of their parents in seed germination and dormancy with relatively high dormancy rate of 41.1%. In summer, F1 hybrid seeds buried in the 3 cm soil exhibited greater viability (52.4%) than those in the soil surface with greater seed longevity (74.6%) than its maternal (63.3%) and paternal (33.7%) parents at 100 days of over-summering in soil. In winter, F1 seeds buried in the soil surface were more viable than those in the 3 cm soil with greater seed longevity (83.5%) than its maternal (39.0%) and paternal (71.7%) parents at 100 days of over-wintering in soil. Therefore, it is concluded that F1 hybrid resulted from gene flow from OSR to mustard has high seed dormancy and longevity during summer and winter, suggesting its weedy risk potential. Further studies are required to examine the reproductivity and fitness cost of F1 hybrid to make a clearer conclusion of its weedy risk.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.86-90
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• 2009

The C. militaris belongs to entomopathogenic fungi, which have their specific sequences in internal transcribed spacer regions (ITS1 and ITS2) depending on species. In this study, to identify the phylogenetic relationship of the strain hybrided by mating of C. militaris, we compared DNA sequences of ITS regions and 5.8S ribosomal DNA (rDNA) repeat unit of hybrid strain and its parental strains. The result revealed that hybrid strains are C. militaris species. In addition, cordycepins produced by hybrid strains and other strains of C. militaris were analyzed by HPLC with 20mM $KH_2PO_4$ of mobile phase and C-18 columns. The result indicated that the strain hybrided by mating produce higher concentration of phytochemical cordycepin than other C. militaris strains.

• Current Research on Agriculture and Life Sciences
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• v.5
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• pp.12-18
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• 1987

This study was performed to investigate the creeping habit in top-cross progeny, and to determine the relationship among the major agronomic characters in the top-cross progeny testing based on the The The conclusions of simple correlation coefficients. the study were summarized as follows ; The creeping type crossed with the non-creeping tester was the greatest in width and seed yield. For all three characters, the creeping type crossed with the non-creeping tester and the non-creeping type crossed with the creeping tester were greater than any other combination. The top-cross method was desirable for the study of general and specific combining ability. The sensitivity of the tester to differentiate the creeping and non-creeping types was better when a non-creeping tester was used.

• Journal of Sericultural and Entomological Science
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• v.36 no.1
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• pp.30-36
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• 1994

choChorion(egg-shell) morphology of the F1 hybrid between Bombyx mori and Bombyx mandarina has been observed by scanning electrom microscope and chorion protein was analyzed by electrophoresis. The chorion surface structure of F1 hybrids in the lateral(flat) region was similar to that of maternal line. The F1 hybrids chorion was found to have basically a three layer structure. The middle and inner layer were very much like those of the Bombyx mandarina and Bombyx mori. There were many conic pillar structures in the outer layer of the F1 hybrid, which was similar to Bombyx mandarina. This conic pillar structure had a thin cover layer was more clear in the dorsal and ventral side of the F1 hybrid chorion. The conic pillar structure of Bombyx mandarina was found to be dominant in F1 hybrid chorion irrespective of their maternal line. Major components of chorion protein were analyzed by two-dimensional electrophoresis and found to have isoelectric points in the range of pH 4.0-6.5 and molecular weight 10 to 50 kd. F1 hybrid chorion protein components related directly to those of the maternal line. The conic pillar structure was dominat characteristic and it was present in all F1 hybrid.

• Current Research on Agriculture and Life Sciences
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• v.20
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• pp.65-70
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• 2002

This study was conducted to investigate allelopathic potential of various crosses of rice cultivars using their inhibitory effect on barnyardgrass growth under field conditions, and to determine PAL activity and phenolic compounds involved in phenylpropanoid pathway from selected crosses of rice cultivars. Under field conditions, Kouketsumochi, Woo co chin yu possessed higher allelopathic potential inhibiting over 90% of barnyardgrass growth. Crosses of Kouketsumochi/Woo co chin yu, Dongjinbeyo/Kouketsumochi, Dongjinbeyo/Woo co chin yu showed over 80% inhibitory effects on barnyardgrass growth. The highest PAL activity, $63.46{\mu}kats/kg$ proteins was detected in Kouketsumochi which is the most important enzyme in phenylpropanoid pathway and also higher PAL activity in crosses with Kouketsumochi. Content of cinnamate was $2.64{\mu}g/g$ f.w. in Kouketsumochi which was 2 to 5 times higher than other rice cultivars tested, indicating that higher PAL activity resulted in more cinnamate. The similar trends in cinnamate content and PAL activity were observed in crosses of rice cultivars with Kouketsumochi.

• Journal of Mushroom
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• v.12 no.3
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• pp.171-180
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• 2014

The primary objective of the present study is the characterization of the hybrids of monokaryon- monokaryon (mono-mono) crosses in mushroom breeding. We employed this technique for developing superior strains from Pleurotus species strains with 85 mono-mono intraspecific hybrids of 7 combinations between six Pleurotus ostreatus strains and one Pleurotus florida strain. In this study, the results of analysis on hybridization rate, nuclear DNA patterns, and colors and yields of fruit-bodies, are presented as follows. The crossability between mono-mono crossing ranges between 50 and 93.75%. The results of the analysis on the nuclear DNA patterns of 85 hybrid strains of mono-mono crosses share the nuclei of both parents, but their genetic similarities were predominated by either parent. The hybrid strain between P. florida and P. ostreatus showed patterns more similar to P. florida, while the hybrid strain between P. ostreatus and P. ostreatus either had patterns predominated by either parent strain. The fruiting body colors of the mono-mono crosses mostly had combined colors of both parents but showed the tendency of being more similar to that of either parent. 82% of the hybrid strain indicated similar fruiting body yields compared to both parent strains, while 0% was higher and 18% were lower than both parents. The present study was able to find out and suggest superior hybrid trains by identifying the nuclear DNA patterns of hybrids between Pleurotus species as well as the characteristics of their fruiting bodies. This study expects that the advantages of the mono-mono crossing are needs to be fully utilized in mushroom breeding and it is better to develop superior strains of Pleurotus species strains together with the mono-mono crossing.