• Korean journal of applied entomology
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• v.11 no.1
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• pp.25-30
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• 1972

Five to seven day old pupae were irradiated with gamma rays, the mating ability, and competability of the irradiated males were examined. The results obtained are summarized as follows: 1. There is no difference in percentage of males that mated, nor in the maximum number of matings of the males, when individual matings were made, and fresh females were provided every day for his life time. 2. The average days for the first mating of the emerged were 1.5 days for the control, and 2.4 days for the males irradiated with 25 and 30 Krad. The average days between first and second mating were 3.0 days for the irradiated males, and 1.3 days for the control. 3. The average matings of a male were 1.2 and 2.5 times when 5 and 10 females were provided to a normal male respectively. No significant different in average numbers of mated female per male were observed between the normal and irradiated males. 4. To test the mating competitiveness of the irradiated and normal males (1:0, 1:1, 2:1, 1:2, 5:1, 1:5, 0:1) were confined together with five virgin females. The greatest reduction in hatching percentage was found with 5 : 1 : 1 (30 Krad) where $26.6\%$ was hatched.5, The results showed that the irradiated males were not fully competitive with normal males, but if the reduced number of eggs produced by the female mated with irradiated male is considered, it might be used sterile male techniques in rice stem borer control.

• Korean journal of applied entomology
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• v.33 no.2
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• pp.66-73
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• 1994

Adult emergence period, the calling behavior pattern and the mating ability of female depending on ages, 9day old male's response to sex pheromone released from each 1- to 5-day old female, and the structure and location of sex pheromone gland were obse~edin Ostrinia furnacalis. Adults usually emerged from 1 hour before to 1-2 hours after the beg~nning of scatophase under the 16L/8D photoperiod. And most active calling behavior was obserued for two hours from the 5th to 7th hours of scotophase among 2- and 3-day old females, and 3-day old male' response to 3-day old females was higher than to any other age of females during the 5th-8th hours of scotophase. Mating frequency was higher at the 4th-7th hours after the beginning of scotophase. Among 1. to Cday old females extruding sex pheromone gland, more than 65% of them successfully mated with 2- to 4-day old males. And, 2- or 3-day old females exhuding sex pheromone gland showed an abdiw. over 60%, to mate with each 1--5-day old males. Hypertrophied cell layers, presumed to be a sex pheromone gland of female adults, were located at two intersegmental membranes between the 8th & 9th, and the 9th & 10th abdominal segments.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.63-70
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• 2012

This study was conducted to investigated the distribution, ecological character and life cycle of Black Soldier Fly(BSF), Hermetia illucens, to determine effects of BSF on composing ability to the livestock feces. The distribution of BSF was defined in all parts of the country in Korea. Its main habitat was found to be areas near cattle sheds, feces sheds, living waste dump grounds, and food waste dump grounds. Excellent species of the bio-conversion using the livestock feces were selected out of two kinds of solider flies(Hermetia illucens and Ptecticus tenebrifer). And effects of BSF on composing ability to the livestock feces were pig feces 0.5 g, cattle feces 0.43 g and chicken feces 0.3 g per a larvae of BSF. In the examination of the ability of BSF to decompose food waste, volume of the livestock feces decreased by weight pig feces 77, cattle feces 83.62 and chicken feces 80%. Observed characteristics of BSF larvae and pupae stage were by developmental stage may be summarized as follows: size and length of larvae and pupae of BSF were higher than in pig feces and mixed sawdust treatments. In order to develop artificial indoor rearing techniques, The pupation and emergence rate were not visible the difference but important factor of the mating and egg-laying was a sun light. And it was visible the difference in mating ratio. Mating rate of H. illucens by seasonal change was the highest from May to July. Mating mostly occurred between 10 : 00 and 12 : 00 during which light intensity is high. As the egg-laying medium for artificial egg collection, calf feed and food waste were most effective. The ratio of egg-laying medium is waste food : sawdust(1 : 1) in volume. For egg-laying materials, flower foam and wood with holes were used; holes sized 3 ~ 5 mm in diameter, 7 ~ 10 mm in depth were most preferred for egg-laying.

• Korean journal of applied entomology
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• v.34 no.3
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• pp.169-173
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• 1995

This study was conducted to investigate control effects against Spodoptera exigua Hubner in field using mating disruption dispensers of synthetic sex pheromone during 1993 and 1994. Denisities in egg and larva of Spodoptera exigua in field treated with the dispensers was 1.9% level of that without dispensers in welsh onion, 12.5% in red pepper and 4.4% in potato in 1993. Also 5.9% and 5.2% were counted from 10 ha and 20 ha of welsh onion field compared. Control value in Fields with dispensers were maintained at 80% level in 10 ha and 90% level in 20 ha until October.

• Korean journal of applied entomology
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• v.9 no.2
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• pp.85-90
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• 1970

This study was conducted to find out the evidence of a sex attraction in the fall webworm moths, Hyphantria cunea D., in the fold conditions. The study dealt with the sex attraction between the sexes, selection of effective trap type, effect of moth age on the sex attractiveness, sex attractiveness after copulation, and the time of the sex attraction in the day, The trap was baited with a living moth in the cylindrical screened case 4cm. in long and 2.7cm in diameter. The traps were attached on the tree in a horizontal position at the height of about 3m. on the ground. A sex pheromone was evident in the female moth which attracted the male to the lure, and the modified sticky trap was most effective in this experiment. The trap consisted of a cylindrical powder milk can 11.5cm. in long and 10cm. in diameter with the entry hole 4.8cm, in diameter at both ends of the can, and the inside of can was lined with paper coated with acri-soil(sodium poliacrilate) to catch them. Males were mostly attracted to females during their first 2 days of life, and its attractiveness decreased with moth age, No males were attracted to females more than five days old, and no females lived for more than six days. After copulation, sexual attractiveness was decreased swiftly, Sexual attractiveness was found to be greatest between 4 and 6 A.M. Living females can be used as effective baits for luring the males to the fold trapt.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.117-122
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• 2000

The chemosterilizing effects of 4 benzoylphenylureas (BPUs), were tested on the adult house fly at different developmental stages. The male and female flies before insemination (I-day-old) or with fully developed ovaries (5-day-old) were exposed to the flask surface coated with BPUs solution (300 ppm) for 24 hrs. None of the eggs layed by the 5-day-old flies on the next day of exposure to either flufenoxuron or teflubenzuron hatched to larvae. The eggs layed by the 5-day-old flies exposed to either triflumuron or diflubenzuron also showed a significantly reduced hatchability of 23%. The first egg batches layed by 1-day-old flies exposed to flufenoxuron showed hatchability of only 1%. These results indicate that the BPUs applied were effective in egg sterilization irrespective of developmental stage of house fly. The effect of flufenoxuron was the most pronounced and lasted for 5 days after exposure.

• Korean journal of applied entomology
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• v.53 no.3
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• pp.209-215
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• 2014

This study was performed to monitor the seasonal occurrence of Pseudococcus comstocki (Kuwana) by using pheromone traps and to test a mating disruption technique to control this pest in pear orchards. We compared the attractiveness of rubber septa loaded with 1.5 and 3.0 mg of the pheromone, 2,6-dimethyl-1,5-heptadien-3-yl acetate. A total of 1,021 and 1,431 males were caught in traps baited with 1.5 mg and 3 mg of the pheromone, respectively. The numbers of males caught were not affected by trap color, although yellow traps were more attractive than white traps. In 2012, P. comstocki males were captured between June and October, with peaks in late June, early August, and late September. In 2013, the males were trapped between June and October, with peaks in middle June, late July, and late September. In pheromone mating disruption tests, catches were reduced by 17.7, 65.3, and 62.9% in orchards treated with 450, 900, and 1,350 mg per 10 a of the pheromone, respectively. At harvest, 3.4, 2.9, and 4.8% of fruits in orchards treated with 450, 900, and 1,350 mg per 10 a were damaged by P. comstocki, while 9.5% were damaged in the control orchard.

• Journal of Embryo Transfer
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• v.12 no.2
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• pp.127-132
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• 1997

본 연구는 rat에서 PMSG도는 FSH 처리에 의한 과배란 유도가 배란율과 수정란의 질에 미치는 영향을 알아보기 위해 호르몬 처리하고 교미시킨 후 4일령에 난관과 자궁을 세척하여 정상 8-세포기 난자와 비정상 난자를 조사하였고 각 처리에서 채란된 난자 중에 정상난자를 골라 체외 배양하여 발육율을 비교 평가하였다. 미성숙 rat에서는 평균19.1개의 수정란이 채취되었으며 성숙rat에서는 14.2개가 채취되었고 미성숙 rat에서는 성숙 rat에 비해 더 많은 비율의 비정상적인 난자가 회수되었다. FSH와 LH-RH에 의한 방법이 PMSG와 HCG에 의한 방법보다 유의성 있게 많은 난자를 배란시켰으며, 비정상란의 빈도도 낮은 것으로 나타났다. 그러나 호르몬 처리에 의한 두 가지 방법은 자연배란에 의한 방법에 비해 훨씬 높은 비정상난자의 배란을 유도하였다(FSH, 20.1%;PMSG, 41.2%;자연배란 13.4%). 또한 FSH처리에 의해 회수된 난자보다 체외 발육율이 높은 것으로 나타났다. 그러므로 rat에서 PMSG와 FSH를 이용하여 과배란을 유도할 수 있으나 배란된 난자의 비정상율은 자연배란에 비해 훨씬 높았고, 과배란 유도시 호르몬의 종류에 따라 체외 배양율에도 영향을 미치는 것으로 나타났다.

• Journal of Sericultural and Entomological Science
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• v.15 no.2
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• pp.9-14
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• 1973

In order to find out effects of the generative power of silk worm moth which have been brought up in the high temperature accommodation at their pupa stage. For this specific study, 9 different kinds of male silk worms have been selected as specimen. All those specimen were brought up in the normal temperature at their larvae stage and after the pupation period they have been accommodated in the condition of high temperature for a certain length in accordance with the study programme. Afterwards, those mlae specimen were copulated with Suwon jam 103${\times}$104 which were all brought up in normal conditions. This study was carried out to find the copulation function as well as the ratio of unfertilized eggs(male sterility test). Results of study have been revealed as follows: 1) Although some differences were observed, male pura which have been brought up in the condition of high temperature shown the low rate of unfertilized eggs rather than those were brought up in the normal conpition. 2) In this group the eclosion(emergency) has been found to be poor rather, than those specimen brought up in normal conditions. 3) The copulation function of Moran, Daedong, J124 and C108 specimen were found to be poor than those of Suwon jam. 4) Fertility rate of Moran, Daedong, J124 and C108 was found to be around 65%. This figure is rather lower than what we normally expect. 5) Unfertilized egg ratio of Moran, Daedong and C108 were found to be around 20% if they were brought up in the condition of high temperature for one day from the time of pupation: 40% at 2 days, and 70% at 3 days duration. More than 3 days treatment has shown no progress in the unfertilized egg ratio. 6) One day's treatment for the pupa at the later stage has shown the unfertilized egg ratio of about 10%; 20% at 2 day's treatment, 35% at 3 day's treatment, 40-60% at 4 day's treatment, more than 60% at 5 day's treatment, and the 70% of fertilized egg ratio was only observed when the treatment days come to 7 days. It was understood that the unfertilized egg ratio was high at the antepupa stage rather than that of post-pupa stage. 7) According to the result of observation the sperm in copulatory pouch and seminal receptacle out of the normal female silk worm which have been copulated with the male brought up in the condition of high temperature at their moth stage. The reproduction system found in the control zone has been found to be normal and the sperm is amountful and active in motion while the sperm found in the treatment to be limited in amount and slow in motion. The observation was made within 5 hours from the copulation. 8) According to the result of observation of sperm of seminal receptacles of the female silkworm moth, and according to that observation of sperm in the seminal receptacle in female silkworm moth, the amount of sperm and mobility in the female moth brought up in high temperature were poor comparing that were brought up in normal temperature zone. Some of them are even found to be no trace of such. 9) Appearance and mosle of the copulatory organ of the male silkworm moth was found to be no anatomical change. 10) Testis of the later pupa stage which was brought up in the high temperature was found to be almost net developed to anucleate sperm and they are degenerated at stage of between maturation division and sperm abnormal stage. Mean time at control zone, the formation of anucleate sperm was already observed.

• Korean journal of applied entomology
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• v.36 no.4
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• pp.323-330
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• 1997

The apple leafminer, Phyllonorycrer ringoniella (Lepidoptera: Gracillariidae), is the most important pest on apple leaves and is not easy to be controlled with insecticides because of leafmining habit of its larvae. This study was undertaken to develop an artificial diet for mass rearing of the moth, and to look at their reproductive behavior. For developing an artificial diet the problem of diet contamination with microorganisms and how to supply artificial diet to first instar larvae have to be solved first. Artificial oviposition trial was successful with shadowy bottomside of various papers sprayed with apple leaf extracts. The highest activity of both calling and mating was observed within 30 min. after the onset of photophase and 3- to 4-day -old adults showed the highest mating rate. A ring-shaped sex pheromone gland was found at the intersegmental membrane between the 8th and 9th abdominal segments of the female. (E, 2)-4,lOtetradecadienyl acetate was identified as the major component from the femall gland extracts by the gas chromatography (GC) and GC-mass spectrometry. Additionally it was thought that (a-10-tetradecenyl acetate could be the second component of their sex pheromone.