• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.12a
• /
• pp.50-50
• /
• 2020

기후변화의 영향으로 자생지에서 고사하고 있는 구상나무 유묘의 대기온도와 연령에 따라 건전도를 파악하고자 지리산 구상나무 실생묘를 대상으로 엽록소형광을 측정하였다. 2020년 7, 8, 9월에 걸쳐 월별 1회씩 연생별(1, 2, 3, 5, 6년생) 20개체를 대상으로 13시, 19시에 개체당 3회 반복 측정하였다. 측정 당시 평균대기온도는 24.6, 26.9, 20.6℃이었으며 잎을 20분간 광을 차단 후 Fv/Fm값을 측정하였다. Fo는 식물이 암적응 된 상태에서 포화광을 조사하기 직전의 형광 초기값이며, Fm은 식물이 암적응 된 상태에서 포화광을 통해 유도된 최대 형광수치를 의미한다. Fv는 Fm-Fo이며, 광화학 반응의 최대 양자수율로 식물의 잎이 광합성을 수행할 수 있는 잠재력을 의미하고 식물의 광합성 활성과 건전도에 대한 지표로 사용된다. 일반적으로 건강한 잎의 Fv/Fm값이 0.8을 기준으로 낮은 값은 광계II의 반응 중심이 손상을 잎은 경우이거나 스트레스 환경에 있는 경우이다. 13시 측정 결과 8월 측정값 중 1년생과 3년생이 0.8 이하로 측정되었고 그 외에는 전부 0.8 보다 높게 나타났다. 19시 측정 결과 8월에 1, 2, 3년생이 0.8 이하로 측정되었으며, 9월에는 1년생의 경우를 제외하고 모두 0.8 보다 높게 나타났다. 1, 2, 3년생은 8월의 높은 온도에 영향을 받아 고산지대에 자생하는 구상나무의 특성상 고온에 대한 스트레스를 받은 것으로 판단되며, 연령이 비교적 높은 5, 6년생은 온도변화에 비교적 안정적인 것으로 나타났다. 따라서 엽록소형광 측정의 결과를 근거로 3년생 이하의 개체는 온도변화에 민감하게 반응하는 반면, 5년생 이상의 개체는 비교적 안정적으로 건전도가 유지된 것으로 판단되었다.

• Horticultural Science & Technology
• /
• v.33 no.6
• /
• pp.829-838
• /
• 2015

Garlic (Allium sativum L.), one of the oldest cultivated crops, is the most widely used Allium species belonging to the family Lilliaceae. In this study, growth characteristics, photosystem II activity, and antioxidative enzyme activity were investigated in five temperatures ($10-30^{\circ}C$) during early growth stage of garlic to determine the optimum temperature for cultivation and assess the effects of high temperature on early growth of garlic. Vegetative growth (e.g., shoot height, number of leaves) of garlic plants was greater in the temperature ranges of $15-25^{\circ}C$. However, dry weight (of shoot, bulb, and total plant) of garlic was significantly greater at $20^{\circ}C$, compared to either below or above $20^{\circ}C$. $F_v/F_o$ and $F_v/F_m$ values were highest at $15-20^{\circ}C$, and decreased above $25^{\circ}C$. The chlorophyll a fluorescence induction OKJIP transient was also considerably affected by high temperature; the fluorescence yields $F_i$ and $F_P$ decreased considerably above $25^{\circ}C$, with the increase of $F_k$ and $W_k$. Activities of catalase and superoxide dismutase in leaves and peroxidase in roots were high in $20-25^{\circ}C$, and decreased significantly in $30^{\circ}C$. These results indicate that a growth temperature of $30^{\circ}C$ inhibits early growth of garlic and that it is desirable to culture garlic plants near $20^{\circ}C$. Fluorescence parameters such a $F_v/F_o$, $F_v/F_m$, $F_k$, $ET_o/CS_m$, and $PI_{abs}$ were significantly correlated with dry weight of whole garlic plants (p < 0.01), indicating that these fluorescence parameters can be used for early assessment of high temperature effects even though the damage to the plant is not very severe.

• Journal of Bio-Environment Control
• /
• v.33 no.1
• /
• pp.45-54
• /
• 2024

A proper level of UV-A light treatment in terms of intensity, spectrum, and exposure duration is known to have a positive impact on plant growth, photosynthesis, and the biosynthesis of secondary metabolites. However, there are few studies investigating the physiological responses of spinach (Spinacia oleracea L.) to UV radiation. Hence, this study aimed to assess the effects of short-term UV-A radiation on the growth and bioactive compounds of spinach. Spinach seedlings were cultivated in a vertical farm module under the following environmental conditions: photosynthetic photon flux density 200 µmol·m^-2·s^-1, white LED, 12 h on/off, 20℃ air temperature, 70% relative humidity, and 500 µmol·mol^-1 CO₂ concentration. After 5 weeks of sowing, the seedlings were subjected to continuous UV-A (peak wavelength; 385 nm) irradiation at two different energy levels: 20 W·m^-2 and 40 W·m^-2 for 7 days. As a result, the UV-A_20W treatment increased the shoot fresh and dry weights of spinach. However, there were no significant differences observed in photosynthetic parameters between the UV-A treatments and the control. The maximum quantum efficiency of photosystem II (Fv/Fm) consistently decreased across all UV-A treatments for 7 days in UV-A treatments. Additionally, the total phenolic content and antioxidant capacity increased in the UV-A_20W treatment at 7 days of treatment as well as the total flavonoid content significantly increased at 5 and 7 days of treatment. These findings suggest that supplemental UV-A LED radiation can enhance the growth and quality of spinach cultivated in closed type plant production systems such as vertical farms.

• Journal of Plant Biotechnology
• /
• v.40 no.3
• /
• pp.156-162
• /
• 2013

Plant tolerance to drought is a beneficial trait for stabilizing crop productivity under water deficits. Here we report that genetically engineered Chinese cabbage expressing Arabidopsis $H^+$-pyrophosphatase (AVP1) shows enhanced physiological parameters related to drought tolerance. In comparison with wild type plants under soil water deficit stress created by cessation of irrigation, soil water potential in pot with AVP1-expressing plants was more rapidly decreased that might lead to increased relative water content in leaves, while both genotypes had indistinguishable wilting phenotypes. Transgenic plants subjected to drought treatment also exhibited higher photosystem II quantum yield in addition to lower electrolyte leakage and $H_2O_2-3,3^{\prime}$-diaminobenzidine content when compared to wild type plants.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.21 no.3
• /
• pp.196-207
• /
• 2019

This study was carried out to investigate the effects of C. japonica, D. morbifera, D. macropodum, I. anisatum, Q. glauca and R. indica To investigate the photosynthetic ability, chlorophyll content, chlorophyll fluorescence analysis, and physiological environmental. The photosynthetic rate, cancer respiration rate, stomatal conductance, and rate of evaporation tended to decrease as a result of drying stress in the no-water condition for 28 days. I. anisatum, Q. glauca and R. indica showed a low rate of less than 40% until 28 days of no-treatment. The total chlorophyll contents were decreased in the order of D. macropodum> D. morbifera> C. japonica> Q. glauca> M. thunbergii> R. indica> I. anisatum. Chlorophyll fluorescence analysis showed that there was no change in the qP, but after 28 days no $Fv/F_m$, $F_o$, $R_{fd}$, $NPQ_{_-LSS}$ can be a useful indicator for quantitative estimation within a short period of time with a marked reduction rate of PSII quantum yield ${\Phi}PSII$ in the rectified state by continuous light during the nominal adaptation period. In the case of I. anisatum, Q. glauca and R. indica If water management can be carried out at intervals, it may be possible to plant trees in trees and landscape trees.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.27 no.3
• /
• pp.15-25
• /
• 2019

The study was conducted to compare the chemical properties and microbial activity of soil and the crop productivity by applying homemade liquid fertilizers (LF) used in leading cucumber farms as well as to evaluate the eco-friendly LFs to substitute for a chemical fertilizer. Three homemade LFs, EM, starfish, and native microbes, and a chemical LF were regularly fertigated per three days during the growing season. Chemical LF contained the highest pH, EC (electrical conductivity), and concentrations of T-N, $P_2O_5$, K, Ca, and Mg, while the lowest EC level was observed for EM LF. Soil EC was the highest to the 3.0 dS/m for chemical LF-plots, with lowering soil pH, OM (organic matter), and Mg concentration. Soil chemical properties mostly increased in native microbes LF-plots. However, soil microbial properties were not significantly different among the LF treatment plots. OTU (operational taxonomic units), richness estimator, and diversity index of bacteria and fungi increased in the chemical LF and EM LF based on the pyrosequencing analysis. SPAD and PS II values on the treated-cucumber leaves were seasonally decreased from 32 to 60 days after transplanting, with the rapid decline observed at 45 days after transplanting. Number of leaves and crop height increased in the treatments with EM and native microbes LF. LF treated-cucumber crops were not significantly different for total fresh weight and fruit yield.

• Korean Journal of Environmental Biology
• /
• v.30 no.2
• /
• pp.98-106
• /
• 2012

The chlorophyll fluorescence and photosynthetic $CO_2$ fixation capacity of leaves from three major crop trees found on Jeju Island, Camellia sinensis L., Camellia japonica L., and Citrus unshiu M., were analyzed. The photosynthetic $CO_2$ fixation rate of C. sinensis was similar to that of C. unshiu, and much higher than that of C. japonica which belongs to the same genus. Stomatal conductance in the three species was high at dawn and low during daytime. The intercellular $CO_2$ concentration of the three species was also high at dawn and decreased at midday. The transpiration rate showed an opposite trend from the intercellular $CO_2$ concentration. The photochemical efficiencies of PSII (Fv/Fm) in C. sinensis were slightly lower at midday compared to the level at dawn and/or dusk. The decline in Fv/Fm of C. sinensis at midday was much smaller than that of C. japonica. These results indicate that C. sinensis is better acclimated to high levels of radiation under natural conditions in late summer, although its PSII reaction center was inhibited by strong radiation. Of the chlorophyll fluorescence parameters in the species, the RC/CS decreased significantly while the ABS/RC, TRo/RC, ETo/RC, and DIo/RC increased significantly at midday in late summer. However, C. unshiu did not show significant changes in these values depending on the time of day. Among the three species, the daily $CO_2$ fixation rate in C. sinensis ($320.1mmol\;m^{-2}d^{-1}$) was the highest, followed by that of C. unshiu ($292.5mmol\;m^{-2}d^{-1}$) and C. japonica ($244.8mmol\;m^{-2}d^{-1}$). Thus, C. sinensis may be a valuable crop tree in terms of the uptake of $CO_2$ under natural field conditions.

• Korean Journal of Organic Agriculture
• /
• v.27 no.1
• /
• pp.87-100
• /
• 2019

The study was initiated to compare crop productivity as affected by a long-term application with homemade liquid fertilizers in leading organic cucumber (Cucumis sativus L.) farms in Suncheon and Kimcheon provinces. A Suncheon farm have applied an EM (effective organism) liquid fertilizer for one year and fifteen years, designating as EM 1-year and EM 15-year plots, respectively, with 4-year and 5-year application of native microbes-liquid fertilizer in Kimcheon farm, designating as Micro 4-year and Micro 5-year plots, respectively. pH in the EM-liquid fertilizer was high to approximately 7.7, and EC in the Micro-liquid fertilizer was 0.1 dS/m higher than those of EM-liquid fertilizer, with similar macro-nutrient concentrations observed in the both liquid fertilizers. Soil EC was the highest to the 10.0 dS/m for the liquid fertilizer with EM 1-year and showed less than 1.5 dS/m for other liquid fertilizer plots. Micro-liquid fertilizer plots had soil OM contents less than 20 g/kg, which was approximately two times less than those of EM plots. Soil microbial properties were not significantly different among the liquid fertilizer plots. SPAD and PS II values were significantly increased by EM 15-year plots with high levels of soil OM and EC. Liquid fertilizer plot with EM 1-year had high concentrations of T-N, Ca, and Na in the cucumber crops but low concentrations of P and Mg, in particular for low K of 1.2% which was two times less than those of desired level for an optimum cucumber growth. The lowest fruit yield was observed for the liquid fertilizer plot with EM 1-year with the highest soil EC accumulated. Liquid fertilizer plot with EM 15-year produced the expanded volume of crop canopy and increased fruit yield. Therefore, long-term of continuous application with an organic liquid fertilizer would have sustainably improved soil stability and the crop productivity.