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Development of One-Step Immuno-Chromatography Assay System for Salmonella typhimurium (Immuno-Chromatography 방법을 이용한 식중독균(Salmonella typhimurium) 1단계 분석시스템의 개발)

  • 백세환;이창우이창섭육순학
    • KSBB Journal
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    • v.11 no.4
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    • pp.420-430
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    • 1996
  • One-step immuno-chromatography assay system for heat-killed Salmonella typhimurium antigens was developed. Three major components used were a glass fiber membrane (placed at the bottom of the system) with an antibody (specific to the analyse, detection antibody)-gold conjugate deposited in a dry state on the surface, a nitrocellulose membrane (middle) with an antibody (also, specific to the analyse but recognized different epitome: capture antibody) and anti-detection antibody immobilized in spatially separated areas, and a cellulose membrane (top) as absorption pad. These membranes were partially superimposed such that a wicking of aqueous solution containing sample can continuously take place through membranes. Variables that affected the system performance were the concentration of capture antibody, the location on the membrane, inert protein used for blocking of the membrane and for carrying the sample, and the concentration of the gold conjugate. Under optimal conditions, within 15 minutes after absorption of a sample solution from the bottom of the system antigen-antibody complexes of sandwich type were formed on the membrane surface area with immobilized capture antibody and a color signal was generated in proportion to the analyse concentration. The minimum do tection limit of the analyse was $1{\times}106$ Salmonella cells/mL.

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Microstructure and Wear Characteristics of TiC-SKD11 Composite Fabricated by Liquid Pressing Infiltration Process (용융가압함침 공정으로 제조한 TiC-SKD11 복합재료의 미세조직 및 내마모 특성)

  • Cho, Seungchan;Jo, Ilguk;Lee, Sang-Kwan;Lee, Sang-Bok
    • Composites Research
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    • v.30 no.3
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    • pp.209-214
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    • 2017
  • Titanium carbide (TiC) reinforced SKD11 matrix composites were successfully fabricated by a novel liquid pressing infiltration process. Microstructure, mechanical properties, and wear characteristics of the fabricated 60 vol% TiC-SKD11 composite are analyzed. The composite exhibits superior mechanical properties, such as hardness and compressive strength with 24% lower density as compared with SKD11. Improved wear resistance of the TiC-SKD11 composite originates from uniformly reinforced TiC having strong interfacial bonding strength between TiC/SKD11 interface.

Application of Acoustic Emission Technique and Friction Welding for Excavator Hose Nipple (굴삭기용 호스 니플의 마찰용접과 음향방출기법의 적용)

  • Kong, Yu-Sik;Lee, Jin-Kyung
    • Journal of the Korean Society for Nondestructive Testing
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    • v.33 no.5
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    • pp.436-442
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    • 2013
  • Friction welding is a very useful joining process to weld metals which have axially symmetric cross section. In this paper, the feasibility of industry application was determined by analyzing the mechanical properties of weld region for a specimen of tube-to-tube shape for excavator hose nipple with friction welding, and optimized welding variables were suggested. In order to accomplish this object, friction heating pressure and friction heating time were selected as the major process variables and the experiment was performed in three levels of each parameter. An acoustic emission(AE) technique was applied to evaluate the optimal friction welding conditions nondestructively. AE parameters of accumulative count and event were analyzed in terms of generating trend of AE signals across the full range of friction weld. The typical waveform and frequency spectrum of AE signals which is generated by friction weld were discussed. From this study the optimal welding variables could be suggested as rotating speed of 1300 rpm, friction heating pressure of 15 MPa, and friction heating time of 10 sec. AE event was a useful parameter to estimate the tensile strength of tube-to tube specimen with friction weld.

A study on the natural history projection mapping contents using Gestalt Visual Perception Theory (게슈탈트 시지각 이론을 활용한 자연사 프로젝션 맵핑연구)

  • Park, Ki-Deok;Chung, Jean-Hun
    • Journal of Digital Convergence
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    • v.18 no.5
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    • pp.465-471
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    • 2020
  • This paper aims to maximize the exhibition effect and to emphasize the efficiency of sample information transfer by using the surrealism dephase network technique and beam projection mapping by aesthetically approaching the arrangement of the existing exhibition specimens in the science museum using natural history contents. It presents the limitations of the existing analog natural history sample information transmission in digital form to give viewers interesting and fun and suggests the direction of using digital diorama for sample exhibition.

SEM 및 AFM을 이용한 한지의 특성 분석

  • 최태호;조남석
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2000.11a
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    • pp.141-141
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    • 2000
  • 한지의 제조에 있어서 부원료인 점질물은 초지시 매우 중요한 역할을 하는데 그 주된 역 할은 다음과 같다. 먼저 점질물은 섬유의 분산을 용이하게 하고 종이의 강도를 증가시키며, 양지와는 달리 박엽지의 제조가 편리하고, 종이의 경도를 증가시키며, 습지의 처리를 용이하 게 하며, 점성으로 인하여 섬유의 침전올 방지하고, 종이의 광택을 향상시키는 둥 매우 중요 한 역할을 한다. 그러나 한지 제조시 이러한 식물성 점제의 미묘한 작용은 현재 대다수의 한지 제조 공장에서 사용하고 있는 합성점제인 PAM이나 PEO 등의 합성 고분자 화합물에 서는 기대하기 어려운 작용이라 여겨진다. 이와 같은 사실에서 본 연구에서는 전통적인 천 연점질물인 황촉규근의 점질물과 합성점제인 PAM 및 우리 나라에서 전혀 사용되지 않고 있는 나무수국 내수피의 점질물을 이용하여 한지를 제조하고 이들 한지의 특성을 SEM 및 A AFM(Atomic Force Microscopy)를 이용하여 분석하였다. 먼저 각각의 점질물로 제조한 한지를 SEM으로 관찰한 결과 닥나무 인피섬유의 최외층에 투명막이 존재하는 사실을 발견할 수 있었다. 이러한 투명막은 닥나무나 뽕나무 인피섬유에 만 존재하고 삼지닥나무나 산닥나무 둥과 같은 기타 인피섬유에는 존재하지 않으므로 한지 의 원료 섬유의 식별에 매우 중요한 요소가 된다. 또한 이러한 투명막은 섬유간 결합을 증 대시켜 한지의 강도 발현에 기여한다고 사료된다. 천연점질물인 황촉규근과 나무수국 점 질물을 이용하여 제조한 한지를 SEM 및 SEM-EDXA를 이용하여 분석한 결과, 황촉규근 점질물로 제조한 한지에는 상당량의 전분입자가 폰재하고 있었으며 나무수국 점질물로 제 조한 한지에는 침상의 수산칼슐 결정이 상당량 존재하고 있는 사실을 발견하였다. 이러한 사실은 한지 제조시 사용된 점질물의 식별에 중요한 요소라 사료된다. 한지의 원료인 닥나무 인피펄프와 침엽수 미표백 크라프트 펄프를 AFM을 이용하여 분석 한 결과, 닥나무 인피펄프의 마이크로피브릴 폭은 5-10nm로 Sw-UKP의 마이크로피브릴 폭 lO-20nm보다 매우 가늘고, 치밀한 세포벽 구조를 하고 있었다. 닥나무 인피펄프의 이러 한 세포벽 구조 및 마이크로피브렬의 형태가 Sw-UKP보다 높은 섬유강도를 나타내는 원인 이라 사료된다. 각각의 점질물을 이용하여 제조한 한지의 섬유표면을 AFM을 이용하여 관 찰한 결과, 원료펄프의 표면관찰에서와는 달리 초지시 사용된 점질물이 섬유표면을 피복하 고 있어 명확한 형태의 마이크로피브렬을 관찰할 수 가 없었다. 따라서 점질물의 이러한 역 할이 한지의 강도 및 보존성 향상에 기여하리라 사료된다.

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AN EXPERIMENTAL STUDY ON THE RESIDUAL STRESS AND BOND STRENGTH OF CERAMO-METAL SYSTEM (치과도재용(齒科陶材用) 합금(合金)과 도재간(陶材間)의 잔류응력(殘溜應力) 및 결합강도(結合强度)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Kim, Gi-Jin;Bae, Tae-Seong;Song, Kwang-Yeob;Park, Charn-Woon
    • The Journal of Korean Academy of Prosthodontics
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    • v.29 no.2
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    • pp.67-84
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    • 1991
  • This study was carried out to investiagate the residual stress caused by the mismatch of thermal expansion and the bond failure resistance of alloy-porcelain specimens. The thermal expansions of alloys and porcelains were measured by using a straight push-rod dilatometer. Porcelain glass transition temperatures, thermal expansion coefficients, and thermal compatibility indices were derived from length-versus-temperature curves. Strain gauges were used to experimentally determine the Young's moduli of porcelains, the residual stresses of porcelain surface, and tensile bond strengths of the specimens of simulated porcelain metal crown. The obtained results were as follows: 1. The coefficients of thermal expansion for alloys were the minimum of $13.53\mu/^{\circ}C$ and the maximum of $20.11\mu/^{\circ}C$ in the range of $100\sim600^{\circ}C$ and those for porcelains were the minimum of $7.72\mu/^{\circ}C$ and the maximum of $31.24\mu/^{\circ}C$ in the range of $100\sim500^{\circ}C$. 2. The glass transition temperature of porcelains exhibited the same value without my relation to the healing rate, and the thermal disharmony of porcelain and alloy was more affected by porcelains than by the alloys. 3. The Young's moduli of body porcelains were larger than those of opaque porcelains(P<0.01) 4. It seemed that the residual stresses of porcelain surfaces in the porcelainalloy systems were more affected by porcelains than by alleys. 5. The bond strengths of the procelain-base metal alloy systems were larger than those of the porcelain-precious metal alloy systems. The fracture strengths of porcelain surfaces showed significant difference between porcelains (P<0.05).

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A Study on the Relationship between the Uptake of $^{67}Ga-citrate$ and the Incorporation Rate of $^3H-thymidine$ and Metabolism of Protein in the Rat Livers Treated with $CCl_4$ (사염화탄소투여(四鹽化炭素投與)후 백서간세포(白鼠肝細胞)에서 $^{67}Ga$섭취율(攝取率)과 $^3H-thymidine$ 결합율(結合率) 및 단백대사(蛋白代射)와의 관계(關係)에 관(關)한 연구(硏究))

  • Hong, Seong-Woon
    • The Korean Journal of Nuclear Medicine
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    • v.19 no.1
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    • pp.83-93
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    • 1985
  • The ability of $^{67}Ga$, administered carrier free as the citrate complex, to localize in human and animal tumors to an extent sufficient to permit visualization of the lesion by scanning is well established. However, neither the mechanism of $^{67}Ga$ uptake by tumors or inflammatory cells nor its relationship to cell type or to the biochemical status of the cell is yet understood. Author investigated the uptake of $^{67}Ga-citrate$ using subcellular tissue fractionation of rat livers treated with $CCl_4$ associated with the $^3H-thymidine$ incorporation rate to detect subcellular localization of $^{67}Ga$ and it's relationship in DNA synthesis. Large amounts of $^{67}Ga$ associated with the soluble portion of tissue homogenate rather than with isolated cell organelles and not related nuclei residue in the regenerating period after hepatocellular injury caused by $CCl_4$. The elevated uptake of $^{67}Ga$ in the livers of $CCl_4$ treated rats was also inhibited when protein synthesis was stopped by cyclohexamide. Thus protein and the soluble portion of issue homogenates seems to play an important role in the elevated uptake of $^{67}Ga$ in liver injury induced by $CCl_4$ treated rats.

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Determination of Serum Iron, Iron-Binding Capacity and Transferrin Saturation in_Health (건강인(健康人)의 혈청철(血淸鐵), 철결합능(鐵結合能) 및 transferrin saturation 측정(測定)에 관(關)하여)

  • Tchai, B.S.
    • Journal of Nutrition and Health
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    • v.3 no.3
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    • pp.141-147
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    • 1970
  • Nutritional anemias are an important nutritional problem affecting large population groups in most developing countries. Many reports on investigations of nutritional anemias have been published and there is good evidence that prevalence of iron deficiency anemias in vulnerable groups is high in many areas of the world. However, there is a general lack of accurate data on its prevalence throughout the world. Methods used to measure different factors have been variable and often of poor quality. Furthermore recent comparison of values obtained in different laboratories have shown a much greater discrepancy in iron binding capacity measurements as compared with serum iron determinations. Since the major cause of these differences appears related to the technique employed, the efforts of the central laboratory will be directed initially to standardization of methodology. My laboratory has been joined interlaboratory comparison of WHO studies of iron standardization. The determination of serum iron, iron-binding capacity and transferrin saturation was done on healthy adults, 79 males and 20 females, who visited hospital for health certificate from January to July 1970. The serum iron was determined by the method of modification of Bothwell and Mallett and total iron blinding capacity by Ramsay method and Bothwell and Mallett method. The results of this study are as follows; 1) The serum iron concentration of seventy nine adult male by the method presented is $131.5{\pm}37.3{\mu}g/100ml\;(range\;52.5{\sim}225.0{\mu}g/100ml)$ and of twenty female adult is $108.5{\pm}40.2{\mu}g/100ml\;(range\;45.0{\sim}202.5{\mu}g/100ml)$. 2) The adult male level of serum iron-binding capacity is $330.9{\pm}48.9{\mu}g/100ml\;and\;adult\;female\;is\;291.2{\pm}55.2{\mu}g/100ml$. 3) The transferring saturation of healthy male is $35.6{\pm}12.8%\;(range\;15.1{\sim}18.0%)\;and\;of\;female\;as\;25.4{\pm}12.4%\;(range\;10.1{\sim}60.0)$.

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전기 화학적 방법으로 성장한 SnO2 나노구조의 광학적 및 전기적 특성

  • Lee, Dae-Uk;Yun, Dong-Yeol;Kim, Tae-Hwan
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.368.2-368.2
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    • 2014
  • $SnO_2$을 이용한 반도체는 기체 센서, 트랜지스터, 태양전지와 같은 여러 분야에 적용 가능하기 때문에 많은 각광을 받고 있다. $SnO_2$을 이용한 반도체 소자는 높은 화학적 안정성과 독특한 물리 화학적 특성을 지니고 있을 뿐만 아니라 부피에 대한 높은 표면적 비율을 가지고 있다. 우수한 $SnO_2$나노구조를 얻기 위해서 전자관 박막증착, 졸겔법, 물리적 증기증착, 열증착과 같은 다양한 방법들이 사용되었다. 다양한 합성 방법들 중에서 전기화학 증착법은 높은 성장율, 대면적 공정, 낮은 가격과 같은 장점을 가지고 있어 많은 연구가 진행되었지만, $SnO_2$ 구조의 성장조건에 따른 체계적인 연구는 진행되지 않았다. 본 연구는 indium-tin-oxide (ITO)로 코팅된 유리 기판 위에 전기화학 증착법을 사용하여 다양한 성장 조건에 따라 성장된 $SnO_2$나노구조들의 물리적 특성들을 관찰하였다. ITO 유리 기판 위에 성장된 $SnO_2$나노구조는 음극의 전구체와 전류의 상호작용에 의해 생성되는 산소 분자의 환원에 의해 형성된다. $SnO_2$나노구조의 모양은 전기화학 증착의 성장 환경에 따라 달라진다. $SnO_2$나노구조를 관찰하기 위해 시간에 따른 전압-전류, X-ray광전자분광법, 주사형전자현미경, X-ray회절분석법을 사용하여 측정하였다. ITO 유리 기판 위에 성장한 $SnO_2$ 소자에 서로 다른 인가 전압을 가해 주었을 때에 따른 전류밀도를 측정하였다. 일정한 인가전압에서 $SnO_2$나노구조의 X-ray광전자분광법 측정 을 통해 화학적 결합과 X-ray회절분석법 통한 $SnO_2$ 성장 방향을 관찰하였다. 주사형전자현미경 측정을 통하여 $SnO_2$의 표면을 관찰하였다

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Evaluation of the Two Class Population Balance Equation for Predicting the Bimodal Flocculation of Cohesive Sediments in Turbulent Flow (난류조건에서의 점착성 유사 이군집 응집 모형 적용성 평가)

  • Lee, Byung Joon;Toorman, E.A.
    • Journal of Korea Water Resources Association
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    • v.48 no.3
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    • pp.233-243
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    • 2015
  • The bimodal flocculation of cohesive sediments in water environments describes the aggregation and breakage process developing a bimodal floc size distribution with dense flocculi and floppy flocs. A two class population balance equation (TCPBE) was tested for simulating the bimodal flocculation by a model-data fitting analysis with two sets of experimental data (low and high turbulent flows) from 1-D flocculation-settling column tests. In contrast to the Single-Class PBE (SCPBE), the TCPBE could simulate interactions between flocculi and flocs and the flocculation mechanism by differential settling in a low turbulent flow. Also, the TCPBE could perform the same quality of simulation as the elaborate Multi-Class PBE (MCPBE), with a small number of floc size classes and differential equations. Thus, the TCPBE was proven to be the simplest model that is capable of simulating the bimodal flocculation of cohesive sediments in water environments and water, wastewater treatment systems.