• Journal of Conservation Science
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• v.34 no.1
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• pp.1-9
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• 2018

The analysis of ancient DNA extracted from archaeological bones has become an important research tool in palaeogenetics and anthropology. Eight human skeletal remains of the Joseon dynasty, excavated from Hwamyeong-dong, were used in this study. DNA was extracted from bone powder using a silica-based protocol. The isolated DNA was analyzed by the sequencing variation of hyper-variable region of the mitochondrial DNA. In the present study, 3 human remains were identified into mtDNA haplogroups including the A 5a, D4a, and M4"67+16311 groups, using HaploGrep 2 program. The identified haplotypes of the 3 samples have been confirmed that the specimens in the tombs were not related by the maternal line. This is the first analysis of human skeletal remains of the Joseon dynasty excavated in Busan. Date from the analysis of human remains from the Joseon dynasty are considered as the basis for understanding the genetic relationship between modern and ancient humans of the Korean peninsula.

• Analytical Science and Technology
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• v.18 no.3
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• pp.257-262
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• 2005

Mitochondrial DNA (mt DNA) sequence analysis has been a useful tool for species identification of animals and human individuals. Two hypervariable regions (HV1 and HV2) in control region of mitochondrial genome were analyzed for human individual identification. In case of animal species identification, several genes on mt DNA such as cytochrome b (cytb), RNAs, cytochrome oxidases (CO) were used. In this study, co-amplification of HV1 and cytb was carried out in order to check the contamination of animal DNA and to verify the human DNA. The primer sets used in PCR were H15997/L16236 for HV1 and H14724/L15149 for cytb. PCR products for HV1 and cytb were 239 bp and 425 bp, respectively. The appearance of two bands on agarose gel implied the DNA came from human, however the single band of cytb gene represented the non-human animal DNA.