• Journal of the Korean Wood Science and Technology
• /
• v.31 no.4
• /
• pp.1-7
• /
• 2003

Anatomical and physical characteristics of damaged wood by forest fire and sound wood of Pinus densiflora were examined. Granular substances were found in resin canals, epithelial cells and ray parenchyma cells of damaged wood, but the other distinctive marks of wood structure in damaged wood were not observed. Damaged wood showed slightly higher relative crystallinity in outermost growth ring than sound wood. Green moisture content and water absorptions both in tangential and radial sections of damaged wood were lower than those of sound wood. There were no significant differences in shrinkage, swelling and basic density between damaged and sound woods.

• Korean Journal of Plant Taxonomy
• /
• v.37 no.2
• /
• pp.87-108
• /
• 2007

The pollen morphology of 31 taxa, representing all woody species from 12 genera of Fabaceae in Korea, was examined by light microscope and scanning electron microscope. The pollen grains were monad and prolate spheroidal or rarely subprolate in equatorial view. The pollen grain aperture was trizonocolporate or rarely trizonooperculate, which does not occur in the other families of Fabales in Korea. The exine showed a great variety of sculpture pattern, i.e. psilate, microperforate, densely microperforate, perforate, polygonal perforate, macroperforate, macroperforate with granules in holes, polygonal macroperforate, verrucate with pitted perforate striae, verrucate-perforate, granulate, rugulate, reticulate or refined reticulate. The pollen morphological data supported the idea that the genera Echinosophora and Sophora were congeneric. Caragana (Galegeae) was quite close to the Sophora group (Maackia, Sophora and Echinosophora) of the Sophoreae in each measured pollen parameter and exine sculpture pattern. Eleven genera of Fabaceae were recognized based on differences in aperture type, pollen grain shape and exine pattern, and a generic pollen key of the woody Fabaceae in Korea was developed. A key to pollen types of Lespedeza was also suggested. The examined taxa of Coronilla and Wisteria were recognized by their exine patterns.

• Journal of Veterinary Clinics
• /
• v.7 no.1
• /
• pp.429-438
• /
• 1990

The cytochemical characteristics of the hematopoietic cells in blood and bone marrow from 3 clinically healthy dogs were examined using a battery of cytochemical stains. Alkaline phosphotase activity was demonstrated in eosinophilic series and occassionally in progranulocytes. A variety of cells exhibited acid phosphatase activity, but tartrate-resistant acid phosphatase activity was seen only in eosinophilic series. Peroxidase activity was observed in myeloblasts to segmented cells of granulocytic series and in monocytes. ${\alpha}$-naphthyl acetate esterase activity was found in monocytes and occassionally in lymphocytes. Naphthyl-AS-D-chloroacetate esterase marked neutrophilic series from myeloblasts to segmented cells. ${\beta}$-Glucuronidase activity was detected in a variety of cells except the cells of erythrocytic series. Periodic acid Schiff stain-positive granules were demonstrated in the neutrophilic and eosinophilic series from myelocytes to segmented cells and in monocytes and occassionally in lymphocytes. Sudan black B stain-positive granules marked granulocytic series from myeloblasts to segmented tells and monocytes.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.16 no.4
• /
• pp.3572-3584
• /
• 1974

본 논문은 Thrower의 양곡성능에 관한 예측 방정식을 차원해석법을 적용하여 유도하는데 있다. Thrower의 능률에 큰 영향을 미친다고 생각되는 인자들간의 관계식들을 Buckingham의 $\pi$이론에 의하여 구하여 졌으며 본연구에 사용한 Thrower는 현재 농촌에서 사용되고 있는 자동탈곡기의 Thrower와 유사한 형으로 제작된 것이며 실험에 사용된 시료는 벼의 경우는 통일벼와 미네히까리 품종이고 보리 품종으로서는 나맥이며, Thrower의 Impeller 직경, 속도 및 투입량을 변화시켜 가면서 각 시료에 대하여 양곡높이를 측정하여 그 예측방정식을 유도하였다. 본 논문에서 얻은 결과는 다음과 같다. 1. Thrower의 양곡성능에 지대한 영향을 미친다고 생각되는 주요 인자들을 Thrower의 양정, impeller의 직경과 속도, 투입량, 곡립의 밀도, 중력가속도등이라 간주하여 Buckingham의 $\pi$이론을 적용하여 이들에 관한 $\pi$항들을 결정하였으며 그 결과는 다음과 같다. {{{{ pi 1= { D} over {H } , pi 2= { { DN}^{ { 1} over {2 } } } over { { g}^{ { 1} over { 2} } }, pi 3= { Q} over { { g}^{ { 1} over {2 } } { rho D}^{ { 2} over {6 } } }}}}} 2. 실험에 의하여 구해진 $\pi$항문의 관계식을 합성하여 Thrower의 양곡성능에 관한 시료별 예측방정식을 구하였으며 이 식들은 아래와 같다. 통일 : $\pi$1=0.1223($\pi$2)-1.1813$\times$($\pi$3)-0.1864 미네히까리 : $\pi$1=0.1185($\pi$2)-0.9663$\times$($\pi$3)-0.1575 나맥 : $\pi$1=0.1599($\pi$2)-1.2350$\times$($\pi$3)-0.1382 3. 현재 농촌에서 사용되고 있는 자동탈곡기체 부착된 Thrower의 회전수, Impeller의 직경 및 투입량은 각각 1,100rpm, 30cm 및 150gr/sec 정도로서 이와같은 수준의 변량들을 기초로 하여 본 논문에서 유도한 예측방정식을 적용하여 Thrower의 양정을 산출한 결과, 통일벼의 경우는 2.6m정도였고 미네히까리 품종과 나맥의 양정은 각각 2.4m, 2.9m 정도였다. 이와같은 사실로부터 1m내외의 양정만으로 되어있는 자동탈곡기 Thrower의 경우 제작비절감, 소요동력 감소를 기하고 아울러 과립의 손상을 줄이기 위해서는 설계 또는 작동조건의 변경을 통해 Thrower의 회전수를 현재 수준이하로 줄일 필요가 있다고 판단되었다.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.59 no.1
• /
• pp.101-108
• /
• 2014

This study was conducted to investigate the effect of nitrogen rates on the growth characteristics and yield of maize in agricultural fields with the stream. This indicates the necessity and optimal level of nitrous fertilization to examine the possibilities of quantity enhancement. Plant height and ear height of maize were not significantly different among the nitrogen rates. Stem diameter and leaf area index increased in the nitrogen treatment compared to untreated control. Changes of photosynthetic rate in maize leaves depending on nitrogen treatments increased as much as nitrogen rates were increased up to the highest level, 36 kg per 10a. NDF and ADF content levels of maize were investigated with different nitrogen rates regardless of treatments. In the case of NDF, it showed a tendency to decrease after 8 days of tasseling date. ADF had also decreased after 15 days of tasseling date. Nitrogen uptake of maize leaves with different nitrogen rates showed the highest level, $4.9g\;kg^{-1}$ with 36 kg per 10a on the tasseling date. Ear length and 100-kernel weight, there were no significant differences according to yield and the components with different nitrogen rates. Ear diameter and kernel number, nitrogen rates of 18 kg and 36 kg were increased compared to nitrogen rate of 9 kg per 10a and untreated control. The pericarps in 9 kg nitrogen rate and control were thicker than those of 18 kg and 36 kg treatment. The yield, 18 kg, 36 kg, and 9 kg treatments were increased by 10.96%, 9.27%, and 3.31%, compared to control. The component analysis on maize kernel with different nitrogen rates, starch showed no significant differences among treatments. Total sugar in 18 kg nitrogen treatment represented the highest content level, 6.37%. In addition, Amylopectin in 18 kg treatment showed the highest content level of 90.38%. However, amylose in 18 kg treatment showed the lowest level, 9.62% which drew a conclusion that waxy of 18 kg treatment is considered to be the strongest one. From the results described above, nitrous fertilization is essential to grow maize in agricultural fields with the stream. The optimum level of nitrous fertilization is considered 18 kg per 10a.

• Clinical and Experimental Reproductive Medicine
• /
• v.28 no.2
• /
• pp.161-167
• /
• 2001

Objective: Granulocyte-macrophage colony stimulating factors known to be secreted in murine and human reproductive tract. The development of human, bovine and murine embryos could be promoted by addition of GM-CSF in culture medium. However, the pregnancy and implantation rate of embryos cultured in GM-CSF have not been evaluated. The aim of this study was to assess the effect of GM-CSF in embryo development, pregnancy and implantation rate. Methods: A total of 191 IVF cycles were divided into control and GM-CSF supplement group (control=96, GM-CSF=95). The embryos were cultured for three day with or without 2 ng/ml of recombinant human GM-CSF. The quality of embryo, developmental velocity, pregnancy and implantation rates were compared. Results: There was no difference in age, number of gonadotropin ampules used, number of oocytes and fertilization. The number of ICSI cycle was higher in GM-CSF group. In GM-CSF group, G-1 grade embryos were the highest in proportion (56.4%), while G-2 grade embryos were highest (44.3%) in control group. The developmental velocity of embryos were not different between GM-CSF and control group. The pregnancy and implantation rates were significantly higher in GM-CSF group than control (47.4% vs. 33.3%, 17.0% vs. 11.1% respectively). Conclusion: By adding GM-CSF in culture medium, the quality of embryo, pregnancy and implantation rate could be improved.

• Polymer(Korea)
• /
• v.31 no.6
• /
• pp.505-511
• /
• 2007

Poly(lactide-co-glycolide)(PLGA) and hyaluronic acid (HA) has been widely used as biocompatible scaffold materials to regenerate tissue. In this present study, we fabricated microporous PLGA and HA loaded PLGA scaffolds by a emusion freeze-drying method. In order to confirm that the release profile of cytokine or water-soluble drugs, we manufactured the granulocyte macrophage colony stimulating factor(GM-CSF) loaded PLGA and HA-PLGA scaffold. All scaffolds were characterized using scanning electron microscope(SEM), mercury porosimeter and wettability measurement. Cell proliferation and viability were assessed by a 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) test. The porosity of HA-PLGA scaffold was greater than 95% with the total pore area of $261\;m^2/g$. The HA-FLGA scaffold exhibited well interconnected pores to allow greater cell adhesion and prolixferation. It was proven by higher cell viability in the HA-PLGA scaffold than PLGA alone. This may be due to the enhanced natural properties and higher water retention capacity of HA.

• Journal of Life Science
• /
• v.21 no.12
• /
• pp.1778-1783
• /
• 2011

Hematopoietic cytokines regulate production of blood cells by stimulating proliferation and differentiation of bone marrow cells. Among these hematopoietic cytokines, called hematopoitic growth factors, glranulocyte-colony stimulating Factor (G-CSF), which regulates growth of neutrophils, is one of important therapeutic factors because cancer patients suffer with neutropenia which is severe reduction of neutrophils after chemotherapy. Two groups of recombinant G-CSF have approved and used for therapeutic purposes and many researches are still on-going to produce recombinant G-CSF by different techniques. We engineered human G-CSF with Bombyx specific endoplasmic reticulum (ER) signal sequence, therefore, secretion of human G-CSF protein was improved in Bombyx mori-origined cell line, Bm5. The Bombyx ER signal sequence and human G-CSF matured protein region chimera was further remodeled at the N-terminus of matured G-CSF protein to understand roles of N-terminus on outer cellular secretion and/or production. Three different mutants were generated deleting three amino acids in non alpha-helical region in N-terminus in order to scan important amino acids for G-CSF secretion. One of 3 different N-terminal deletion mutants showed dramatically reduction of secreted amount of G-CSF indicating its important role on secretion. The data suggest that remodeling in non alpha-helical region of N-terminus is also important for recombinant G-CSF production.

• Korean Journal of Environmental Agriculture
• /
• v.27 no.2
• /
• pp.178-184
• /
• 2008

This work was carried out to investigate the effect of waterlogging on the growth and nutrient contents of 'Campbell Early' and 'Kyoho' grapevines under the vinyl house condition from June 14 to July 20, 2005. For the trial, seedlings of two-year-old grapevine were transplanted to 40 L pot with a sandy loam soil. Irrigation point of non-waterlogging(control) treatment was controlled at -40 kPa of soil water tension using tensiometer and waterlogging treatments were imposed for 35 days at the water levels of above 10 cm from the soil surface using tap water. The growth of aerial(shoot length, leaf number and stem diameter) and underground(root) parts of 'Campbell Early' and 'Kyoho' grapevines tended to be wholly reduced by waterlogging, while the growth of aerial parts were more severely impaired in 'Kyoho' than in 'Campbell Early' cultivar. The different responses for waterlogging between two grapevines seem to be related with the capacity for absorbing mineral nutrients, because nitrogen content of 'Campbell Early' cultivar leaves was significantly higher than that of 'Kyoho' cultivar although the contents of phosphorus and potassium in leaves of two grapevine cultivars were similarly declined. There was no significant different of fruit quality, such as contents of soluble solid, titratable acidity and weight of berry in 'Campbell Early' between waterlogging and control. In 'Kyoho' cultivar, however, berry weight and titratable acidity were decreased and soluble solid content was increased by waterlogging. It was assumed that waterlogging stress for grapevines promotes maturation and coloring processes of berries by stimulating maturation hormone such as ethylene. In conclusion, 'Campbell Early' cultivar seems to be more tolerable than 'Kyoho' cultivar when comparing the growth responses and nutrient contents between two grapevine cultivars under waterlogging.

• Journal of fish pathology
• /
• v.23 no.1
• /
• pp.47-55
• /
• 2010

This study was conducted to investigate the effects of dietary seleno-yeast on histological response in juvenile olive flounder Paralichthys olivaceus. Fish averaging $4.0{\pm}0.1g$ (mean ${\pm}$ SD) were fed one of the four semi-purified diets containing 0.56, 1.07, 2.86 and 4.56 mg Selenoyeast/kg diet ($Se_{0.56}$, $Se_{1.07}$, $Se_{2.86}$ and $Se_{4.56}$, respectively) in triplicates for 12 weeks. Swelling of blood cell in glomerulus, the rupture of some epithelial cell in the renal tubules and enlarged macrophage were observed in the $Se_{1.07}$ group. The hepatopancreas had hepatic cell, capillary and zymogen in the pancreas and was normal in the $Se_{0.56}$ group. Swelling of hepatocyte gradually decreased with increase in selenoyeast supplementation. The gill lamellae showed partial abnormal condition (terminal clubbing) in the $Se_{0.56}$ group, but there were little differences between these and other treatments. These results indicated that the optimum dietary supplementation level of selenoyeast in juvenile olive flounder, Paralichthys olivaceus, could be less than 1.07 mg selenoyeast/kg diet based on histological responses of the fish.