• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.3
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• pp.1-8
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• 2019

Steel elevator pit was developed for the purpose of minimizing the excavation, simplifying the construction of the frame and economical efficiency by improving the problems that occurred in the existing reinforced concrete. It is common to apply conventional RC method through excavation to underground structures such as underground floor collector well and elevator pit. In recent years, the use of steel collector well and steel elevator pits to reduce construction costs by minimizing the materials of steel and concrete has been continuously increasing. The steel elevator pit is an underground structure and then the performance of the welding part and the structure system is important. Specimen with only steel plate and concrete without studs could support the load more than 3 times than the specimen with deck only. Therefore, even if there is no stud, the deck (steel plate) rib is formed and the effect of restraining the steel plate and the concrete during the bending action can be expected. However, since sudden fracture in the elevator pit may occur, stud bolt arrangement is necessary for the composite effect of steel plate and concrete. It is expected that the bending strength can be expected to increase by about 15% or more depending with and without stud bolts.

• Clinical and Experimental Reproductive Medicine
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• v.37 no.2
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• pp.99-113
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• 2010

Objective: It has been recently reported that very small stem cells with pluripotency are detected in murine and human. The purposes of this study are to confirm whether very small putative stem cells (VSPSCs), which have the proper characteristics of stem cells as well as the expression of stem cell markers, are detected in human endometrium. Methods: The endometrial cells of 5 women, which were obtained by endometrial biopsy, were cultured for 2 weeks and were confirmed for the expressions of alkaline phosphatase, OCT-4 and CXCR4 by immunochemistry. Subsequently VSPSCs were separated by percoll density gradient method and were cultured. Also VSPSCs and their derived cells were confirmed for the expressions of OCT-4 and CXCR4. Results: The colonies, which is composed with VSPSCs less than 3 ${\mu}m$ and the 5~15 ${\mu}m$ sized hyperchromatic round cells, were detected in the endometrium of all of women and showed the strong expressions of alkaline phosphatase, OCT-4 and CXCR4. In culture after the separation of VSPSCs by percoll, these cells showed the morphological and functional characteristics of stem cells; self-renewal, colony formation, embryoid body-like formation and differential plasticity. VSPSCs formed gradually the 5~15 ${\mu}m$ sized hyperchromatic round cells and the 10~20 ${\mu}m$ sized sphere-shaped cells by cell-to-cell aggregation or cell fusion. Then these cells differentiated the various cells including fibroblast-like cells, nerve-like cells and endothelium-like cells. VSPSCs and their derived cells often showed the strong expressions of OCT-4 and CXCR4. Conclusion: VSPSCs less than 3 ${\mu}m$ and their derived cells are detected in human endometrium and these cells have the proper characteristics of stem cells and the expressions of stem cell markers as alkaline phosphatase, OCT-4 and CXCR4.

• Archives of design research
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• v.19 no.1 s.63
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• pp.89-98
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• 2006

As the concept of city changes city is viewed in the perspective of business to make profits, not in the perspective of physical space. In the case of Korea urbanization processed over the level of 80% and the formation of identity is suggested for the way to improve city competitiveness as of globalization and liberalization. Seoulentered the level of developed countries in the perspective of functions and facilities, but its image is evaluated poor because of the lack of cultural competitiveness, tourismattractiveness and service system. Dependency of public transportation which is public facilities representing city indirectly is very high, but in information service and visual systems of pubic transportation operations and management are not enough and differentiated image and loyalty are hard to find. In 2004 government of city of Seoul suggested modifications, but compared to London, Paris and Tokyo consistent system, the lack of control and management of use environment of design, excessive visual information and destruction of connection are pointed out. Therefore to solve these problems the introduction of design system is needed to develop identity after applying characteristics and regulations for using to disordered visual stuff. These procedurescan be explained as City Identity Program (CIP) and this study tries to embody city image through public transportation design system. As the study method for this the status of public transportation design system of London, Paris and Tokyo were searched and compared to that of Seoul. Based on this systematization of symbol marks per public transportation being used right now, the development of design guidelines to form identity, relation of design and the strategy of systematization per method and media were proposed.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.30 no.3
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• pp.103-113
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• 2018

Tide-surge characteristics of the West/South domestic coasts were analyzed with a tool of EST (empirical simulation technique). As a result, stations of Incheon, Gunsan, Mokpo and Busan are categorized as tide-dominant coasts, while Yeosu, Tongyoung and Busan are as surge-dominant coasts. In the tide-dominant coasts, extreme sea level of less than 50-yr frequency is formed without typhoon-surge, while only 10-yr extreme sea level is formed in the surge-dominant coasts. As the results of casual condition of extreme sea level formation considering the relative degree of surge on tide, the regional characteristics were detected also. Three methods for estimating the design tide level were compared. The AHHW method shows an unrealistic outcomes of the concern of over estimate design. Furthermore, the probability distribution function method has been concerned as causing missing data if a huge typhoon occurs in a neap tide or a low tide. To cope with these drawbacks, the applicability of the EST method is proved to be suitable especially in tide-dominant coasts.

• Applied Chemistry for Engineering
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• v.9 no.3
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• pp.348-354
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• 1998

Cellulose grafted with acrylic acid(AAc) was prepared by radiation grafting technique. The effects of reaction temperature. reaction time, monomer concentration, and the crosslinkers on the AAc grafting reaction on cellulose were examined. The amount of AAc grafted on the cellulose reached maximum at the concentration of 0.75vol% difunctional crosslinker and 1.0vol% trifunctional crosslinker, respectively. In the presence of acid, the amount of AAc grafted on the cellulose was decreased when reaction solution contains difunctional crosslinker, while that was increased when reaction solution contains trifunctional crosslinker. In the grafting reaction of cellulose with AAc and TMETA, mixture containing ferrous sulfate and acid enhanced further AAc grafting yield than mixture containing ferrous sulfate only.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.4
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• pp.129-134
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• 2009

As a result of improved wastewater treatment facilities, the point source pollution emitted from human and municipal wastes is greatly decreasing. Conversely, the non-point source (NPS) pollution emanated from city streets, rural homes, suburban development, animal feedlot, croplands, and forestry is rapidly increasing. Practically, the main concern of the government is to control NPS pollutants by means of establishing a long term plan in order to protect the aqua-ecosystem. Studies have been conducted to assess the intensity of NPS from various landuses. In Korea, the data on NPS pollutant loadings are limited to few and broadly categorized landuses unlike in USA wherein specific landuses are available. This research aims to characterize the wash-off characteristics of NPS pollutants from forest landuse. Two sites were monitored during 15 storm events from 04/2008 to 10/2008. Mean $BOD_5$ EMCs are 1.13 mg/L and 0.91 mg/L for the two sites, respectively. The results of this research will be a helpful contribution for the assessment of total NPS pollutant loadings.

• Journal of the Korean Vacuum Society
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• v.10 no.2
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• pp.189-193
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• 2001

We have characterized physical and electrical properties of W-TiN stacked gate electrode structure with TiN as a diffusion barrier of fluorine. As the $N_2/Ar$ gas ratio increased during sputter deposition, TiN thin films became N-rich, and the resistivity of the films increased. However, the resistivity of W-TiN stacked gate reduced as a result of the crystallization of tungsten with the increase of $N_2/Ar$ gas ratio. On the other hand, tungsten in W-TiN stacked gate structure have the (100)-oriented crystalline structure although TiN films were subjected to annealing at high temperature (600~$800^{\circ}C$). Leakage currents of W-TiN gate MOS capacitors were less than $10^{-7}\textrm{/Acm}^2$ and also were lowered by the order of 2 compared with those of pure W gate electrode.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.497-501
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• 2005

근래 들어 도시화로 인한 물순환 파괴를 근본적으로 연구하고 이를 정상화하기 위한 체계적이고 통합적인 유역관리 기술이 요구되고 있다. 유역통합관리의 이론을 실제로 적용한 계획을 세우기 위해서는 Heathcote(1998)가 제안한 다음과 같은 단계별 절차를 수행하는 것이 바람직하다. 단계별 절차는 (1) 대상유역에 대한 정보수집, (2) 문제점 도출, (3) 분명한 목적 수립, (4) 대안의 구성, (5) 대안의 검토, (6) 대안의 효과분석, (7) 최종계획 수립으로 이루어져 있으며 본 연구에서는 3단계의 과정에 해당된다. Heathcote(1998)는 대안의 선정과 평가를 위한 방법으로 단순평가방법(simple assessment method)과 세부평가방법(detailed assessment method)으로 구분하였는데 본 연구에서 수행한 내용은 단순평가방법에 해당된다. 본 연구는 안양천 유역에서 물순환 건전화의 핵심을 건천화 방지로 판단하고 이를 위한 대안을 크게 지표수, 지하수, 대체수자원 분야로 구분하여 제시하였다. 지표수 분야의 경우 구조적인 대안으로 소규모 저수지의 개발, 타유역에서의 도수, 보를 이용한 수량 확보, 우오수분리벽을 이용한 상류유출수의 확보, 저협수로 형성, 하천바닥에 차집관로 매설 방지 및 차집관로 유지관리 등이 있으며 비구조적인 대안으로는 기존 저수지의 유지용수 공급을 위한 운영, 유수지의 다목적 활용, 하천수 직접취수 제한 등이 있다. 지하수 분야의 대안으로는 지하철 용출수의 재이용, 지하수의 과다이용 제한, 침투증진시설의 설치 등이 있으며 대체수자원 분야의 대안으로는 하수처리수를 고도처리하여 유지용수로 재이용하는 것, 중소규모 하수처리장의 상류 설치, 대규모 사업장의 폐수를 고도처리하여 활용하는 것, 상수도의 이용 등이 있다. 이러한 여러 가지 대안들은 Walesh(1989)가 제안한 절차에 따라 기술가능성(technical feasibility), 경제성(economical efficiency), 환경성(environmental feasibility) 등을 정성적으로(qualitatively) 파악하여 실현가능한 대안을 선정하였다. 이렇게 선정된 대안들은 중유역별로 검토하여 효과가 있을 것으로 판단되는 대안들을 제시하는 예비타당성(Prefeasibility) 계획을 수립하였다. 이렇게 제시된 계획은 향후 과학적인 분석(세부평가방법)을 통해 대안을 평가하고 구체적인 타당성(feasibility) 계획을 수립하는데 토대가 될 것이다.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.1-6
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• 2011

Inefficiency of in vivo gene transfer using currently available vectors reflects a major hurdle in cancer gene therapy. Both viral and non-viral approaches have been described to improve gene transfer efficiency but suffer from a number of limitations. Here we tested an adenovirus carrying the small peptide ligand derived from heregulin${\beta}$ EGF-like domain onto fiber, the adenoviral capsid protein, to deliver transgene to ovarian cancer cells which overexpress ErbB, the cognate receptors for heregulin. The attachement of 53 amino acids to fiber didn't affect on the fiber's trimer structure that is critical for the viral entry to cells. The fiber-modified adenovirus can mediate entry and expression of a ${\beta}$-galactosidase into cancer cells in an increased efficiency compared the unmodified adenovirus. Particularly, the gene transfer efficiency was improved up to 5 times in OVCAR3 cells, an ovarian cancer cell line. Such transduction systems hold promise for delivering genes to ErbB receptor overexpressing cancer cells, and could be used for future cancer gene therapy.

• Journal of Science Education
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• v.37 no.1
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• pp.79-86
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• 2013

The purpose of this study was to investigate the middle science teachers' perceptions on science lessons with experiments. The study conducted a survey for 110 science teachers participating in inservice program. The middle school science teachers taught classes with lectures rather than with experimental activities. They had an experiment one to three times a semester in their lessons. They did an experiment to follow to what the textbook said, or to confirm it, like a cookbook style. The most teachers answered that they had over average ability to teach both lecture and experiment. Through the experiment, they expected students had interests and curiosity on science. Despite the advantages of experiments, there were many constraints to conduct experiments, such as teachers' excessive work, laboratory conditions, education environment, and others. It was demanded on continuous interest and investment in the variation of educational environment so that teachers can experiment more often.