• Proceedings of the KIEE Conference
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• 1999.07d
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• pp.1660-1663
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• 1999

혈액 내의 요소 농도는 신장병 진단의 척도로서 정확한 측정이 필요하다. 요소의 농도는 전위차법을 이용하여 정확히 검출할 수 있으며 이를 위하여 효소를 고정화시킨 전극형 센서를 사용하였다. 전도성 고분자로서 P3MT(Poly(3-methylthiophene) )와 PPy(polypyrrole)를 효소 고정화에 이용하였다. PPy는 전기적 특성이 좋고 중합 및 효소의 고정화가 용이하며 중합 과정이 상대적으로 신속하고 비용도 저렴하다는 장점은 있으나. 다소 불안정하다. P3MT는 PPy와 마찬가지로 전극 상에 단량체가 전기적 산화에 의하여 중합되고 일반적으로 전해질 이온이 도우핑된 상태나 도우핑 되지 않은 상태 모두에서 산소. 습도. 온도에 대하여 매우 안정하다. 본 연구에서는 3-methylthiophene과 pyrrole을 전기 중합하여 urease를 고정화한 요소센서의 특성(감도, 안정성, 직선성)을 비교하였다. P3MT를 이용한 센서와 PPy를 이용한 센서 각각에 대해 감도는 P3MT가 32.3mV/decade, PPy가 4.7mV/decate로서 P3MT가 우수하였고 직선성도 보다 뛰어났으며 순환 전압 전류 곡선을 분석한 결과 P3MT가 PPy보다 안정성도 우수한 특성을 보였다.

• Applied Chemistry for Engineering
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• v.5 no.2
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• pp.321-326
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• 1994

The water-soluble polyphosphazene possessing ether side groups was exposed to ${\gamma}-rays$ to prepare hydrogens with good water-swellability. The physical strength of these hydrogels could be controlled by irradiation dose of ${\gamma}-rays$. Trypsin and water-soluble polyphosphazene were irradiated together by ${\gamma}-rays$ for entrapment of enzymes into hydrogel networks. The activities of immobilized trypsin were examined spectrophotometrically after the reaction with N-${\alpha}$-benzoyl-1-arginine-p-nitroanilide in phosphate buffer. The immobilized trypsin was found to have good activity yield and suability after at least 500 hours.

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.2
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• pp.246-251
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• 2010

Impactions can occur because of malpositioning of the tooth bud or obstruction in the path of eruption. However, the exact mechanism is still unknown. The impaction of mandibular first molar is rare with prevalence rates of 0.01~0.25%, but it is important to deimpact the tooth as soon as possible to avoid complications such as dental caries, root resorption, and periodontal problems on the adjacent teeth. Several biomechanical strategies have been proposed for uprighting mesially tipped mandibular first molars. However, most of these have had problems with movement of the anchorage unit because of the reciprocal force. The recent development of skeletal anchorage system(SAS) allows direct application of precise force systems to the target tooth or segment, producing efficient tooth movement in a short time. In this case, an impacted mandibular left first molar with dilacerated roots was treated with a miniplate, which provided skeletal anchorage to upright the tooth. The miniplate was installed in the mandibular ramus, and 10 months after the application of orthodontic force, the impacted tooth was exposed in the oral cavity and uprighted. At this point, the mandibular left first molar was included in the orthodontic appliance with fixed mechanotherapy, the tooth could achieve a normal occlusion. Therefore, the use of SAS simplified the orthodontic procedures and reduced the orthodontic treatment period, and had few side effects.

• The Journal of Korean Academy of Prosthodontics
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• v.57 no.3
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• pp.254-262
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• 2019

As implant can be covered by National Health Insurance Service (NHIS), it was increased the interest in the removable partial denture (RPD) with the surveyed fixed prosthesis supported by implant fixture. To achieve predictable result, it needs the prudent implant planning by basic principles of RPD and patient's residual ridge. This 67 years old age male had a few unilateral remaining teeth, and hoped the treatment covered by NHIS. As using CAD software, the position of implant is planned with regarding to occlusal table of provisional denture, basic principles of RPD, and resorbed residual ridge. The definitive prostheses can ensure the stability and retention of removable prosthesis. When planning implant fixed prostheses, the digital technique was utilized to consider basic principle of RPD and resorbed residual ridge. As a result, it provided satisfactory prostheses.

• The Journal of Korean Academy of Prosthodontics
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• v.61 no.2
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• pp.125-134
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• 2023

When the patient with class III malocclusion needs extensive oral rehabilitation due to multiple missing teeth, accurate diagnosis, and careful analysis, such as the patient's occlusal relationship, facial changes, and evaluation of the temporomandibular joint are essential. Orthognathic surgery is often performed for aesthetic improvement, depending on the patient's chief complaint. If it is not possible due to certain circumstances, partial aesthetic improvement can be achieved through minimal elevation of the vertical dimension. As this patient may have unexpected issues, such as temporomandibular joint disorder, oral habits like bruxism, and masticatory muscle tension, it was determined whether the patient could adjust to a reversible temporary removable partial denture. After this, the maxillary implant-supported fixed prostheses and the mandibular fixed prostheses were used to achieve stable posterior support and to partially improve the maxillary anterior esthetics. The patient was satisfied with the results both aesthetically and functionally. The prognosis is expected to be good if regular check-ups are conducted.

• The Journal of Korean Academy of Prosthodontics
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• v.61 no.4
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• pp.284-292
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• 2023

Stable posterior occlusal support is crucial for adequate masticatory function and facial aesthetics. In elderly patients over the age of 65, masticatory ability has a significant impact on nutritional intake and overall health. This case report presents a prosthetic treatment of an elderly patient with edentulous maxilla and bilateral posterior edentulous mandible. The upper jaw was restored with a complete denture to establish an ideal occlusal plane, and the three-dimensional positions of the mandibular implants were determined accordingly. The implants were placed through computer-guided implant surgery and were immediately loaded with fixed provisional restorations. The implant-fixed zirconia final restorations were inserted in the lower posterior region, and the occlusal surface of posterior artificial teeth in the upper denture was substituted with cobalt-chrome alloy to resist occlusal wear. The patient's posterior occlusal support and masticatory function were promptly restored through this treatment process, and long-term stability against wear was also obtained.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.251.1-251.1
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• 2010

바이오디젤은 동식물성 기름과 메탄올의 트랜스에스테르화 반응에 의해 생산되는 지방산메틸에스테르(FAME, fatty acid methyl esters)로서, 트랜스에스테르화 공정에는 KOH, NaOH, $NaOCH_3$등의 균질계 화학촉매를 이용한 방법, 무촉매 공정인 초임계 메탄올 이용 방법, 그리고 효소촉매를 이용한 방법이 있다. 초임계 공정은 에너지 소비와 장치비가 커서 경제성이 떨어지는 것으로 보고되며 화학촉매 공정은 반응 효율이 높다는 장점을 가지고 있지만, 반응 및 정제단계가 복잡하고 정제과정에 폐수를 발생시키는 문제점을 가지고 있다. 고정화 효소를 사용하는 효소 공정은 에너지 비용의 절감, 후 처리 공정의 단순화, 고 순도의 글리세롤을 얻을 수 있는 장점이 있지만, 반응 속도가 느리고 효소 가격이 비싸다는 단점이 있어 현재까지 상업화되지 못하고 있다. 반응속도가 높고 재사용이 가능한 효소 촉매 공정 개발을 위해 본 연구에서는 Candida rugosa, Rizhopus oryzae 2종을 실리카에 동시 고정화하였다. 고정화 Lipase의 제조는 실리카겔을 과산화수소를 이용하여 전처리를 하고 Acetone과 3-APTES의 혼합용액을 첨가한 후 실리카겔과 (silanization)을 진행 하였다. 그리고 glutaraldehyde를 첨가 하여 공유 결합을 형성 한 후에 증류수를 사용하여 실리카겔을 회수하여 lipase(Rizhopus oryzae, Candida rugosa 10% 용액)를 고정화 하였다. 고정화 효소의 효소 활성을 측정한 결과 3000-3500 Unit(${\mu}mol/g{\cdot}min$)으로 측정되었다. 제조된 고정화 효소를 이용하여 Canola Oil을 바이오디젤로 전환하는 실험을 진행하였으며 생성물로부터 고정화 효소를 분리한 후에 상층의 에스테르층을 취하여 수세한 뒤 원심분리하여 FAME 함량을 측정한 결과 83%의 바이오디젤을 얻을 수 있었다. 그리고 효소 촉매 트랜스에스테르화 반응의 Enzyme, Water, Methanol 투입량의 반응 변수들에 대하여 반응표면분석법(Response Surface Methodology)을 적용하여 최적 반응조건을 도출하는 연구를 수행하였다.

• The Journal of the Korea Contents Association
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• v.18 no.4
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• pp.77-88
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• 2018

In this study, we conducted an experiment with the 219 undergraduates to find out the controlling effects of the verbal anchoring (low, high, non) in the nostalgia ads and the consumers' responses according to the product types (emotional/rational). The results suggest the higher the standards of the effects of the verbal anchoring are, the more favorably the preferences to the ads and the purchasing intention are formed regardless of the existence of the nostalgia. The following reveals that the controlling effects of the verbal anchoring in the nostalgia ads have differences between the purchasing intention and the preferences to the ads as to both the nostalgia familiarity and the product types. In the preferences to the ads in case that the standards of the effects of the verbal anchoring are low, the differences between familiarity group and non familiarity one are not noticeable but in the purchasing intention the differences between the low case and non of the standards of the effects of the verbal anchoring are a little more noticeable.

• KSBB Journal
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• v.4 no.3
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• pp.229-234
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• 1989

NAD+ analogs, 8-( 6-aminohexyl) aminonicotinamide adenine dinucleotide and N6-[(6- aminohewl)-carbamoylmethyl]- NAD+, were imobilized on bovine caseins by the action of hansglutaminase. It appears that NAD+ analogs bind with $\alpha$S1-and $\beta$-caseins through formation of the r-glutamylamine bond between the amino groups attached to the hexyl chains in NAD+ analogs and the glutaminyl residues in caseins. The NAD+ analogs immobilized on the caseins were enzymatically reducible by alcohol dehydrogenase. $\beta$-Casein was more useful carrier than the $\alpha$S1-casein and 8-substituted NAD+ analog was more effective than N6-substituted one in immobilization. Michaelis constant of 8-substituted NAD+ analog immobilized on $\beta$-casein in alcohol dehydrogenase reaction was similar to that of free from of NAD+ and that of NAD+ analog. Immobilized NAD+ was much more stable at alkaline pH than free NAD+ and its analog while maximum velocity was reduced to 31% of the free NAD+ analog. The coenzyme casein conjugated was recovered almost completely in casein precipitated by calcium.

• Applied Biological Chemistry
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• v.35 no.5
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• pp.410-414
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• 1992

Endoinulase from Streptomyces sp. S56 was immobilized by adsorption on DEAE-cellulose in 0.01 M citrate-sodium phosphate buffer, pH 6.0 and the properties of immobilized and free enzymes were investigated. The immobilized enzyme preparation, having 40 inulase activity units per dried matrix, revealed the maximal activity at $pH\;4.5{\sim}5.5$ and $55{\sim}60^{\circ}C$ and were most stable at pH 6 and 45^{\circ}C$. The immobilization caused a drop in optimum pH and affinity toward inulin, a slight increase in optimum temperature, an important increase in thermal stability and maximum reaction velocity. The immobilized endoinulase hydrolyzed the tuber extract of jerusalem artichoke and inulin, mainly into fructose and inulobise, degrading 63 and 78% of the total sugar respectively, within 48 hrs in batch reactor.