• Proceedings of the Korean Geotechical Society Conference
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• 1999.11a
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• pp.21-38
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• 1999

• Journal of the Speleological Society of Korea
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• v.15 no.16
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• pp.61-71
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• 1987

동굴지역은 고생대상부에 속하는 선계의 대석회암통의 말기인 정선석회암층이 널리 분포하고 있다. 이 석회암층은 회백색세일이 개재하고 있는 지질구조로 되고 있다. 이 지층은 지질년대를 편년에 의하여 계산하면 대략 4억∼5 억년에 해당되는 오래된 지층이다.(중략)

• The Monthly Diabetes
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• s.261
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• pp.63-63
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• 2011

당뇨병환자는 자율신경병증에 외해 변비로 고생하기 십상이다. 당뇨뿐만 아니라 주변에서도 변비환자를 많이 볼 수 있는데, 이번 스트레칭은 복부의 내장기관에 자극을 주고 척추 전체를 단련시켜주어 변비 증세를 완화시키는 효과가 있다.

• Journal of the Speleological Society of Korea
• /
• v.14 no.15
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• pp.9-41
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• 1987

우리나라에는 1,000여개에 달하는 동굴이 있다. 그 중에서도 규모가 큰 동굴만도 300여개에 달하고 있다. 그 대부분이 고생대 대석회암통에 속하는 지질층에 발달하고 있어 대체로 구조지질의 생성년대는 약 4～5억년 전의 것으로 알려져 있다. (중략)

• 건강소식
• /
• v.30 no.9 s.334
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• pp.12-13
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• 2006

즐거웠던 휴가가 끝나고 나면 생각지도 못한 고통이 찾아와 우리를 고생시키곤 한다. 수면 부족, 불면증, 근육통.... 그러나 말은 못해도 변비의 고통이 가장 크지 않을까? 변비약 등으로 일시적 증상 완화 보다는 규칙적인 운동과 균형있는 식사로 변비를 탈출해 보자.

• Proceedings of the Costume Culture Conference
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• 1996.08a
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• pp.51-53
• /
• 1996

• 건강소식
• /
• v.36 no.5
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• pp.23-23
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• 2012

늘 먹는 것들이지만 알고 보면 우리 몸을 건강하게 하는 것들이 참 많다. 양배추, 마늘, 식초, 옥수수도 그렇다. 손상된 위벽을 보호하고 소화를 돕는 것들이다. 꾸준히 챙겨 먹도록 하자.

• Applied Biological Chemistry
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• v.41 no.1
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• pp.18-22
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• 1998

A mutant strain which hyperproduced thermostable ${\alpha}-amylase$ was obtained by chemical mutagenesis of Bacillus licheniformis. The mutant strain, SK-5, produced the enzyme about 50 times higher than the original strain. The mutant was longer and slimmer in shape, slower in growth compared to the original strain. Nucleotide sequence analysis of the SK-5 ${\alpha}-amylase$ gene revealed no changes in the the structural gene. The changes found in the promoter region might be responsible for the hyperproduction of the enzyme by the mutant. No structural changes in the enzyme structure could be observed when the secreted enzymes at various culture times were analyzed by Western blot.

• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.525-530
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• 1998

These studies were carried out to select the active grow hairy root lines induced from various ginseng(Panax ginseng C. A. Meyer) parts. Hairy roots were induced in root explants, stem and petiole in vitro by A. rhizogenes R1000 or A. rhizogenes $A_4$. These hairy roots could be grown on the phytohormone free medium, and PCR analysis of rol C and vir C gene fragments confirmed that hairy roots were transgenic tissues. We have selected 11 hairy root lines with active growing characters among 300 hairy root lines selected based on growth and morphological characteristics on 1/2MS solid media with 250 mg/L carbenicillin. Morphological characteristics of selected 11 hairy root lines were thickness and thiness of main roots, and many projection for lateral roots, active grow of lateral roots. Among selected 11 hair root lines prominent characteristics of hairy roots with active growing characters were thiness of main roots and active grow of lateral roots. But characteristics of low growing hairy roots were thickness of main roots and low grow of lateral roots. Finally we have selected actively growing hairy roots, KGHR-1, KGHR-5, KGHR-8 among 11 hairy root lines.

• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.101-109
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• 2004

In order to enhance the productivity of AVM $B_{la}$ produced by Streptomyces avermitilis as a secondary metabolite, we established a basic protocol necessary for protoplast fusion with high-producing strains as a fusion partner, and then obtained various kinds offusants by adopting a massive strain-development procedure (a miniaturized strain screening system). An alternative fusion method using UV and/or NTG mutation of protoplasts was developed to screen genetic recombinants without specific selectable markers. In this method, the mutants obtained by protoplast fusion after UV and/or NTG treatment (95% death rate) of the respective fusion partner (protoplasts of the respective mutants resistant against L-isoleucine antimetabolites such as O-methylthreonine and/or azaleucine) were regarded as DNA-recombined protoplast fusants. Notably it was demonstrated that most of the protoplast recombinants obtained by the UV mutation method were able to biosynthesize higher amount of AVM $B_{la}$ , reaching almost three times higher level (almost equal to the industrial productivity), compared to the average AVM Bla amount of the parallel mother strains.