• KSBB Journal
• /
• v.18 no.4
• /
• pp.289-293
• /
• 2003

Doxorubicin is an anthracycline-family polyketide compound with a very potent anti-cancer activity, typically produced by Streptomyces peucetius. In order to increase doxurubicin productivity, a semi-industrial doxorubicin-producing Streptomyces strain named BR-Dox was cultured in a R2YE liquid medium containing CaCO$_3$, and then converted to a cell wall-free protoplast using lysozyme treatment method, followed by PEG-mediated cell wall regeneration. Among several protoplast-regenerated Streptomyces BR-Dox strains, two independent isolates named BR-Dox4 and BR-Dox6 were visually selected using thin layer chromatography (TLC) based on the pigment overproducing phenotype. Comparing with Streptomyces BR-Dox parental strain, two protoplast-regenerated strains, BR-Dox4 and BR-Dox6 exhibited 25.2％ and 12.2％ higher doxorubicin productivity analyzed by high pressure liquid chromatography (HPLC), respectively. This result suggests that a protoplast-regeneration of an antibiotics-producing Streptomyces strain should be a promising strain development approach for antibiotics overproduction in Streptomyces species.

• Journal of the Korea Society of Computer and Information
• /
• v.20 no.1
• /
• pp.237-246
• /
• 2015

In this paper, Improved productivity models of container terminal are provided by utilizing a simulation considering Tandem-lift Quay Crane and transport vehicle of container's high productivity 'Alternative ship-to-yard vehicles.' Feature of this method is deriving the data of tandem-lift Quay Crane and Alternative ship-to-yard vehicles, estimating the productivity model of tandem-lift Quay Crane by using regression analysis. Tandem-lift Quay Crane is equipment of loading and unloading to increase productivity approximately by 2 time existing (single, twin) Quay Crane by dealing with four 20ft containers or two 40ft containers at the same. Alternative ship-to-yard vehicles can transfer containers(4TEU) more than existing Yard Tractor. This paper is deriving the optimal combination showing the highest productivity by using simulation considering Tandem-lift Quay Crane and Alternative ship-to-yard vehicles on container terminals and developing estimating model of productivity by using regression analysis using data of simulation.

• KSBB Journal
• /
• v.20 no.5 s.94
• /
• pp.376-382
• /
• 2005

Avermectin (AVM) $B_{1a}$ produced by Streptomyces avermitilis via polyketide pathway is a secondary metabolite with powerful anthelmintic and insecticidal activities, thus being used as an efficient agent in the field of agriculture and animal health. It has been reported that a precursor for AVM $B_{1a}$ biosynthesis was isoleucine and the biosynthetic pathway of AVM $B_{1a}$ was closely similar to that of fatty acid. Based on understanding of the biosynthetic pathway of AVM $B_{1a}$, we intended to screen various mutants resistant against O-methyl threonine (OMT), an isoleucine-anti metabolite, and/or mutants resistant against p-fluoro phenoxy acetic acid (pFAC), an inhibitor of fatty acid biosynthesis. It was inferred that these mutants could produce AVM $B_{1a}$ more efficiently, due to the acquired capability of not only overproducing isoleucine intracellularly but also channelling metabolized carbon-sources into the polyketide pathway, thus leading to enhanced biosynthesis of AVM $B_{1a}$. The resulting mutant (PFA-1 strain) resistant against 100 ppm of pFAC was able to produce approximately 42 fold higher amount of AVM $B_{1a}$ compared to the parallel mother strain (4,200 vs. 100 units/l). In addition, through the process of continuous strain improvement program carried out by gradually increasing the OMT concentration, it was possible to obtain a more attractive mutant with greater AVM $B_{1a}$ production capacity (9,000 units/l). Notable was that significantly higher producer (12,000 units/l) could be selected through further screening of the resistant mutants, this time, to even higher concentration of PFAC. Meanwhile, through the analysis of AVM Bla production histograms (i.e., number of strains according to their AVM $B_{1a}$ biosynthetic ability) for the earlier strains in comparison with the high producers having the characteristics of resistance to OMT and pFAC, it was found that production stability of the high-yielding producers were remarkably improved, as demonstrated by the fact that larger proportion of the mutated strains had greater capability of AVM $B_{1a}$ biosynthesis ($71\%$ in the range between 5,000 and 7,000 units/L; $47\%$ in the range between 6,000 and 7,000 units/l). Based on these consequences, it was concluded that the rational screening strategy based on the understanding of the biosynthetic pathway of AVM $B_{1a}$ was very effective in obtaining high-yielding mutants with the features of enhanced production stability.

• Korean Chemical Engineering Research
• /
• v.47 no.2
• /
• pp.220-229
• /
• 2009

Strain improvement and morphology investigation in bioreactor cultures were undertaken in suspended cultures of Phellinus linteus mycelia for mass production of protein-bound polysaccharides(soluble ${\beta}$-D-glucan), a powerful immuno-stimulating agent. Phellineus sp. screened for this research was identified as Phellinus linteues through ITS rDNA sequencing method and blast search, demonstrating 99.7% similarity to other Phellinus linteus strains. Intensive strain improvement program was carried out by obtaining large amounts of protoplasts for the isolation of single cell colonies. Rapid and large screening of high-yielding producers was possible because large numbers of protoplasts ($1{\times}10^5{\sim}10^6\;protoplasts/ml$) formed using the banding filtration method with the cell wall-disrupting enzymes could be regenerated in relatively high regeneration frequency($10^{-2}{\sim}10^{-3}$) in the newly developed regeneration medium. It was demonstrated that the strains showing high performances in the protoplast regeneration and solid growth medium were able to produce 5.8~6.4%(w/w) of ${\beta}$-D-glucan and 13~15 g/L of biomass in stable manners in suspended shake-flask cultures of P. linteus mycelia. In addition, cell mass increase was observed to be the most important in order to enhance ${\beta}$-D-glucan productivity during the course of strain improvement program, since the amount of ${\beta}$-D-glucan extracted from the cell wall of P. linteus mycelia was almost constant on the unit biomass basis. Therefore we fully investigated the fungal cell morphology, generally known as one of the key factors affecting cell growth extent in the bioreactor cultures of mycelial fungal cells. It was found that, in order to obtain as high cell mass as possible in the final production bioreactor cultures, the producing cells should be proliferated in condensed filamentous forms in the growth cultures, and optimum amounts of these filamentous cells should be transferred as active inoculums to the production bioreactor. In this case, ideal morphologies consisting of compacted pellets less than 0.5mm in diameter were successfully induced in the production cultures, resulting in shorter period of lag phase, 1.5 fold higher specific cell growth rate and 3.3 fold increase in the final biomass production as compared to the parallel bioreactor cultures of different morphological forms. It was concluded that not only the high-yielding but also the good morphological characteristics led to the significantly higher biomass production and ${\beta}$-D-glucan productivity in the final production cultures.

• KDI Journal of Economic Policy
• /
• v.11 no.1
• /
• pp.71-94
• /
• 1989

본고(本稿)에서는 대기업(大企業)과 중소기업(中小企業)간의 합리적(合理的) 생산분업체제(生産分業體制)를 논의(論議)하기 위한 참고자료(參考資料)로서 후진적(後進的) 상황(狀況)에서 출발하여 효율적(效率的) 분업체제(分業體制)를 형성하게 된 일본하청제(日本下請制)의 전개과정(展開過程)과 하청론(下請論)의 주요논점(主要論點)을 개괄적(槪括的)으로 정리(整理)하였다. 하청제(下請制)의 전개과정(展開過程)에는 일본(日本)에 특수(特殊)한 경제적(經濟的) 사회적(社會的) 문화적(文化的) 요인(要因)들이 작용하였지만, 산업기형발전(産業技衡發展)과 시장규모확대(市場規模擴大)에 의한 분업(分業)의 확대(擴大) 심화(深化), 분업체제(分業體制)의 재편과정(再編過程)에 일관(一貫)된 경제적(經濟的) 합리성(合理性)과 경쟁원리(競爭原理)의 관철(貫徹), 분업집단전체(分業集團全體)로서 고생산성(高生産性)과 환경적응성(環境適應性)을 실현하는 기업간(企業間) 연결조직(連結組織)의 특성(特性) 등은 일본적(日本的) 조건(條件)과 무관한 경제논리(經濟論理)로 설명될 수 있다.

• Proceedings of the KSME Conference
• /
• 2007.05a
• /
• pp.1243-1248
• /
• 2007

In recent, rapid manufacturing (RM) technology is widely used to develop an injection mould with a high performance. The objective of this paper is to develop the injection mould with a high productivity using a hybrid RM technology combining Laser-aided Direct Metal Tooling process with a machining process. The geometry decomposition has been utilized to improve the speed of the manufacturing for the mould. Mould with conformal cooling channels has been designed to improve cooling characteristics. Several experiments have been carried out to evaluate characteristics of the mould manufactured from the hybrid RM technology. In addition, injection molding tests have been performed to examine the performance of the manufactured mould. The results of the injection molding tests have been shown that a cooling time and the injection time of the designed mould are reduced to one-fifth and one-second that of the mould with convention cooling channels.

• Applied Chemistry for Engineering
• /
• v.5 no.6
• /
• pp.911-916
• /
• 1994

Hollow fiber membrane has been successfully developed as an artificial kidney device in the 1970's. In the early 1970's animal cells were introduced into a hollow fiber membrane cartridge and well propagated in the cartridge. Since then, hollow fiber membrane was utilized as a bioreactor in order to immobilize enzymes as well as to culture microbial cells and plant cells. In this review, the present status and the prospect of hollow fiber membrane bioreactor are investigated in view of cell density and product productivity.

• KSBB Journal
• /
• v.14 no.3
• /
• pp.351-357
• /
• 1999

A marine bacterium Bacillus cereus ASK202, agarase producing strain, was treated with some mutagenic agents, ultraviolte(UV), 1-methyl-3-nitro-1-nitrosoguanidine(NTG), and ethyl methane sulfonate(EMS), several times for the increasing of the agarase production After mutagen treatment, we isolated one mutant strain treated with NTG showed the highest stability and agarase productivity and named as Bacillus cereus ASK202-N3. This Bacillus cereus ASK202-N3 strain was well grown in the modified marine medium containing 0.5%(w/v) agar, 0.3%(w/v) yeast extract, and 5.0%(w/v) NaCl, and the optimal initial pH, temperature and culture time were 7.8, $25^{\circ}C$ and 32h, respectively. In the optimal culture conditions, the agarase production was increased to 5.3 fold(850units/L) compared to that of the wild type.

• Design & Manufacturing
• /
• v.12 no.2
• /
• pp.51-56
• /
• 2018

Generally, The women was used in the cosmetic cushion fact. It has developed with the consideration of manufacturing. In this study, we designed green-friendly and element parts lower and single cushion fact containers using a single material. Injection mold simulation were performed using on 3D design data. The injection mold simulation used the data (Injection time / Cooling time / Temperature / Pressure) in the injection mold parameters. In addition, the sink mark phenomenon in the simulation results is analyzed as a problem due to the thickness and further research is needed in the future.

• Proceedings of the Korean Society Of Semiconductor Equipment Technology
• /
• 2004.05a
• /
• pp.138-146
• /
• 2004

현재 반도체 및 LCD 제조분야에 적용되는 공정장비, 이송시스템에는 고성능, 고생산성이 요구되고 있는 추세이다 기존의 선형이송시스템은 구동원으로 서보모터(Servo Motor)를 사용하고 있으며, 기계적 동력전달장치를 사용하여 직선운동을 하는 구조로 되어있어 마찰소음, Position Error 등의 발생요인이 많은 단점을 가지고 있다. 반면에 선형전동기(Linear Motor)를 이송시스템의 구동원으로 사용하면 기계적 동력전달장치가 생략되어 기존의 선형이송시스템이 가지고 있는 단점들의 개선효과가 크며 유지보수면에서도 유리한 점이 많다. 본 연구에서는 2,500[N]급 영구자석 여자 횡자속 선형전동기를 설계, 개발하였으며, 이를 적용한 추진모듈을 개발하였다. 본 연구에서 개발된 횡자속 선형전동기 적용 추진모듈은 LCD 제조분야의 Stocker, RGV, Index, OHS, OHT 등을 비롯하여 타 산업분야의 선형이송시스템으로의 가능하다.