• Title/Summary/Keyword: 고사기

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Expression of Bcl-2 Protein in Ischemia-Reperfused Myocardium of Rabbit (가토 허혈-재관류 심근에서의 Bcl-2 단백의 발현)

  • 류재욱;김삼현;서필원;박성식;최창휴;류경민;김영권;박이태;김성숙
    • Journal of Chest Surgery
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    • v.31 no.10
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    • pp.924-927
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    • 1998
  • Background: Myocardial cell death after myocardial infarction or reperfusion is classified into necrosis and apoptosis. Bcl-2 protein is a cytoplasmic protein, which inhibits apoptosis and is expressed in acute stage of myocardial infarction but not in normal heart. This study was performed to investigate whether Bcl-2 protein was expressed respectively to the reperfusion time. Materials and methods: Thirty nine New Zealand white rabbits weighing 1.5-4.8 kg (mean, 2.9kg) were alloted into 7 groups (n=5 in each group) which underwent left anterior descending coronary artery(LAD) occlusion for 30 minutes, followed by reperfusion. The animals were sacrificed at 1, 4, 8, 12, 24 hours, and 3, 7 days after occlusion. Ventricle was excised immediately after intervention. Tissues were fixed in 10% buffured formalin and embedded in paraffin. Bcl-2 protein was detected by immunohistochemical stain with using monoclonal antibody against Bcl-2 protein. Results: The positive immunohistochemical reactivity for Bcl-2 protein was observed in 12, 24 hours, and 3 days reperfusion groups. Bcl-2 protein was detected in salvaged myocytes surrounding the infarcted area. Conclusions: Bcl-2 protein is expressed at the late acute stage of infarct. Therefore, the expression of Bcl-2 protein may not protect acute cell death, but may play a role in the prevention of late cell death after myocardial is chemia-reperfusion.

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Characteristics of Flowering and Fruit According to Bearing Branch length and Flower Bud Position in Apples (사과의 결과지 길이와 꽃눈 위치에 따른 개화 및 과실 특성)

  • Kim, Ho-Cheol;Hong, Dae-In;Bae, Jong-Hyang;Leem, Kyu;Kim, Tae-Choon
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.264-268
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    • 2007
  • Flower and fruit characteristics, in 'Hongro' and 'Galaxy Gala' apples, were investigated by bearing branch lengths and flower bud position such as blow 5 cm, $10{\sim}15cm$ and above 20 cm length with terminal bud, and above 20 cm length with axillary bud for investigation on possibility of alternative use of long bearing branch and axillary bud in case of die-back of spur flower bud. In flowering characteristics by flower bud position in the above 20 cm length, the terminal bud was later and was short in flowering period, and also was little in number of flower per bud. Fruit weight, number of seeds, and sugar-acid ratio, in characteristics of ripening fruit, were more the blow 5 cm and $10{\sim}15cm$ length, but soluble solid and malic acid contents was the opposite tendency. In fruit characteristics by flower bud position in the above 20 cm length, the terminal bud had high tendency expected far number of seed in 'Hongro' and Hunter a value of 'Galaxy Gala' apple but had not significant difference. Correlation between fruit weight and number of seed was high. As the results, value of alternative use of long bearing branch in apples had a little expected for fruit weight, especially was more 'Galaxy Gala' and the axillary bud.

Yield of Paeonia Radix and Changes of Paeoniflorin Concentration in Paeonia Radix with Different Growing Stages (작약 생육시기에 따른 약근수량 및 Paeoniflorin함량 변화)

  • ;Kwang-He Kang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.397-404
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    • 1994
  • This experiment was conducted to determine best harvesting time and observe paeoniflorin and some chemical component concentrations of 4-year-old paeonia radix in Euisung and Milyang varieties. Concentrations of paeoniflorin and some chemical components were investigated at 8 periods from Feb. to Oct. Yield of paeonia radix was investigated in Aug. 19, Sep. 18 and Oct. 17. Paeoniflorin and some chemical components were changed seriously at May as flowering time and stabilized after June. The lowest dry matter ratio was 23% and the maximum shrinkage ratio was 47% in May during all the growing stages. Especially, the difference of paeoniflorin concentrations between Euisung and Milyang was about 2%, and paeoniflorin concentrations in Milyang were higher than in Euisung variety during all the stages. Serious infection of disease in October, stem length, stem number per stock and healthful stem ratio in October were more reduced than in August. Yield of paeonia radix was not different from August to September, but that of October harvest was obviously reduced by root-decay-disease. Reasonable harvesting time in 4-year-old paeonia radix was late August to mid-September. When infected shoot rate by disease were severely high, early harvest was more stable in the yield and quality aspects.

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Studies on Radiation Protection Effect of the Beer (맥주의 방사선방어효과에 관한 연구)

  • Sohn, Jong-Gi;Ha, Tae-Young;Hwang, Chul-Hyan;Lee, Young-Hwa
    • The Journal of Korean Society for Radiation Therapy
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    • v.19 no.2
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    • pp.83-90
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    • 2007
  • Purpose: In this study, it was investigated whether commercially produced beer is able to prevent a lymphocyte from radiation induced apoptosis. Materials and Methods: Whole blood samples were acquired from 5 healthy volunteers (male, 26$\sim$38 years old) and the lymphocyte were isolated by density gradient centrifugation. Radiation induced apoptosis of the lymphocyte were investigated by 0.5 Gy, 1.0 Gy, 2.0 Gy, 3.0 Gy to 5.0 Gy irradiation. In some experiments, the donor drunk beer and then blood samples were collected. In other experiments, melatonin or glycine betain was added to lymphocyte culture medium. Treated or untreated lymphocytes were cultured for 60 hours and radiation induced apoptosis of the lymphocyte was analyzed by annexin-V staining through flow cytometery. Results: Relative radiation induced apoptosis ratio of the untreated lymphocytes is 1.22$\pm$1.1, 1.22$\pm$1.1, 1.38$\pm$1.0, 1.47$\pm$1.1, 1.50$\pm$1.2 by radiation dose of 0.5 Gy, 1.0 Gy, 2.0 Gy, 3.0 Gy and 5.0 Gy respectively. Relative radiation induced apoptosis ratio of lymphocytes is isolated from beer drunken donors is 0.97$\pm$1.0, 0.99$\pm$1.0, 1.11$\pm$0.9, 1.29$\pm$1.1, 1.15$\pm$1.1 by radiation doses respectively which are reduced 21.5% compared with untreated lymphocyte. Relative radiation induced apoptosis ratio of the lymphocytes is isolated from non-alcohol beer drunken donors is 1.22$\pm$1.1, 1.17$\pm$1.1, 1.13$\pm$1.3, 1.38$\pm$1.2, 1.32$\pm$1.1 by radiation dose of 0.5 Gy, 1.0 Gy, 2.0 Gy, 3.0 Gy and 5.0 Gy respectively which are reduced 10.8% compared with the untreated lymphocyte. Conclusion: As a result, it is suggested that beer may protect the lymphocyte from radiation damage and inhibit apoptosis.

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An Implementation of Crossward Game using Speech Recognition and Synthesis System (음성인식 및 합성을 이용한 십자말 게임의 구현)

  • Kim Dong-Ju;Youn Jeh-Seon;Lee Young-Ju;Kim Dong-Hwan;Hong Kwang-Seok
    • Proceedings of the Acoustical Society of Korea Conference
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    • autumn
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    • pp.29-32
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    • 2001
  • 본 논문에서는 연구실에서 만든 음성인식기와 합성기를 이용하여 십자말 게임을 구현하였다. 십자말 게임에는 고사성어 600개 정도의 단어가 사용되었으며, 다른 영역별 사전을 추가 할 수 있도록 만들어졌다. 구현된 게임은 시작, 진행 등의 모든 과정이 음성으로 동작하며, 부과적인 정보는 음성 합성(TTS)에 의해 이루어진다. 십자말 게임에 사용되는 단어의 배열은 매번 랜덤하게 선택되도록 구성되며, 음성 인식기는 VCCV (Vowel + Consonant + Consonant + Vowel) 기반의 화자독립으로 구현되었다. 선택된 문제에 대한 설명은 텍스트로 보여주면서, 동시에 TTS 시스템에 의해 음성으로 출력된다.

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Uptake Patterns of N and P by Reeds (Phragmites australis) of Newly Constructed Shihwa Tidal Freshwater Marshes (시화지구 인공습지에서 갈대에 의한 질소 및 인 흡수)

  • 노희명;최우정;이은주;윤석인;최영대
    • The Korean Journal of Ecology
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    • v.25 no.5
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    • pp.359-364
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    • 2002
  • This study was conducted to examine the seasonal pattern of N and P uptake by reeds (Phragmites australis) planted in newly constructed Shihwa tidal freshwater marshes. Reed and soil samples were collected from the wetland periodically from June 2000 to May 2002. Reed samples were analyzed for dry weight and content of N and P Soil organic matter content and salinity were also determined. Dry matter content of reed increased during the growing season but decreased in the fall and winter. However, this seasonal pattern was not so evident in the second year. In particular, throughout the measurement period, dry matter content of reed was lowest at a site showing high soil salinity. Regression analyses between dry matter content of reed and soil EC(1:5) suggested that dry matter content per unit square meter would decrease by 1.5 kg with every 1 dS m/sup -1/ increase in soil EC(1:5). The amount of N and P assimilated by reed significantly decreased from the fall and was lowest in the spring. Net decrease in N content from reed during the fall and next spring was calculated as 34.5 and 24.6 g m/sup -2/ in the first and second years, respectively, while the corresponding P loss was 4.0 and 1.8 g m/sup -2/. Soil organic mailer content increased in the fall and winter, but decreased in the spring and summer. The results of this study suggested that the removal of N and P by reed would be considerable during the growing season but the nutrients taken up by reeds would return as detritus to the marshes in the fall and winter. Based on the results of the study, therefore, the harvest of the reed at the latter part of the growth would be recommended to prevent further water quality degradation. However, the long-term effects of reed harvest needs further study.

Effect of Trace Elements in Alcohol Beverages on the Type of Radiation-induced Cell Death (인체 임파구세포에서 X-선 조사에 의한 세포사의 형태에 주정성분이 미치는 영향)

  • Sohn, Jong-Gi
    • Journal of Radiation Protection and Research
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    • v.35 no.2
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    • pp.49-56
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    • 2010
  • Developments of radioprotective agents are important issues for minimizing the troubles and the effective treatments in radiotherapy. But few agents are useful in clinical and practical fields. It was shown that trace elements in alcohol beverages might have radioprotective effect. In this study, the types of cell death of lymphocytes according to the commercial alcohol beverage was investigated. Normal healthy volunteers ingested distilled water, beer or soju containing $81.5mg{\cdot}dl^{-1}$ ethyl ahcohol, respectively. After 2 hours, their blood were sampled with their consents. Fraction of lymphocytes was isolated by density gradient method with Histopaque-1077 (Sigma) and irradiated with dose from 0.5 to 5 Gy. After 60 hour incubation, the cells were harvested and analysed by flow cytometry. Cell viability was decreased by dose dependent manner. Cell viability of beer group was reduced about 15% compared with control group. Apoptosis in soju group was reduced about 20% compared with control group. Apoptosis of beer and control groups are similar. Necrosis of soju group significantly increased about 35% compared with control group. Early apoptosis of beer group was increased compared with control group. Early apoptosis of soju group was decreased about 25% compared with control group. Late apoptosis of beer and control group was increased by dose dependent manner. Late apoptosis of soju group was increased about 20-30% compared with control group. Late apoptosis of soju was increased and the radioprotective effect of soju was minimal because late apoptosis induced the cell necrosis. In case of soju trace elements, total cell apoptosis was decreased about 20% and early cell apoptosis was remarkably low. In this case, mitotic cells death may be dominant mechanism. Therefore, trace elements in soju may not be effective radioprotective agents.

Evaluation of Cold Tolerance of Blueberry (Vaccinium corymbosum L.) and Diagnosis of Freezing Injury Using Timber Moisture Meter (블루베리의 내한성 평가 및 목재수분계측기를 이용한 동해피해 진단)

  • Kim, Ki-Deog;Lee, Jun-Gu;Ryu, Myeong-Sang;Yoo, Dong-Lim;Kwon, Young-Seok;Lee, Jong-Nam
    • Journal of Bio-Environment Control
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    • v.21 no.4
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    • pp.354-361
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    • 2012
  • This study was conducted to evaluate on the freezing tolerance of introduced blueberry cultivars in Korea and to investigate availability of portable timber moisture meter for simple and rapid diagnosis of blueberry-shoot damage by freezing during wintering. Frost tolerance of blueberry cultivars showed big difference that rates of blueberry-shoot death were widely distributed from about 0% to 100% after wintering. Optical density in TTC reduction of blueberry twig treated low temperature was low in order of $-40^{\circ}C$ < $-21^{\circ}C$ < $4^{\circ}C$. Hardiness evaluation of visible injury in the cross-sectional surface color did not agree with that of rates of blueberry-shoot death during wintering. Lowest water content of blueberry stem measured by timber moisture tester during wintering was about 15%. During wintering, water contents of blueberry stems were higher at lower part of tree, but were low at end part of stems, and then when the blueberry grew again for spring, the water content gradually increased to 20~40%. Water content of blueberry stem with freezing injury during wintering decreased to under 5% by desiccation. Therefore it is assummed that the moisture content of blueberry stem injured by freezing during wintering was about under 14%, and it is expected that portable timber moisture meter could be available for rapid diagnosis of blueberry freezing injury in field.

Influence of Drought Period in Different Growth Stage on Agronomic Characters in Sesame (참깨 생육기별 한발기간이 주요형질에 미치는 영향)

  • 최형국;김용재;구자옥;최원열;김학진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.4
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    • pp.295-303
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    • 1990
  • In this study, drought period when important agronomic characters of sesame plant is critical, was examined at different growth stages. Plant death by drought started at 40 days after drought in vegetative growth stage and 20 days, in reproductive growth stage. Obserbed by growth stage, drought damage in reproductive growth stage was more in jurious than vegetative growth stage. All the important agronomic characters was refreshed until 40 days and 20 days after drought in vegetative growth stage and reproductive growth stage respectively, but it could berefreshed after those times. Decrease rate of yield by drought ranged from 29 to 80% in vegetative growth stage and from 49 to 85% in respective growth stage. All the important agronomic characters except rate of ripeness showed positive association with grain yield under drought condition. Oil content in grain was decreaced by drought but composition of fatty acid was not affected by it.

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A Programmable Multi-Format Video Decoder (프로그래머블 멀티 포맷 비디오 디코더)

  • Kim, Jaehyun;Park, Goo-man
    • Journal of Broadcast Engineering
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    • v.20 no.6
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    • pp.963-966
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    • 2015
  • This paper introduces a programmable multi-format video decoder(MFD) to support HEVC(High Efficiency Video Coding) standard and for other video coding standards. The goal of the proposed MFD is the high-end FHD(Full High Definition) video decoder needed for a DTV(Digital Tele-Vision) SoC(System on Chip). The proposed platform consists of a hybrid architecture that is comprised of reconfigurable processors and flexible hardware accelerators to support the massive computational load and various kinds of video coding standards. The experimental results show that the proposed architecture is operating at a 300MHz clock that is capable of decoding HEVC bit-stream of FHD 30 frames per second.