• Korean Journal of Environmental Agriculture
• /
• v.17 no.3
• /
• pp.254-259
• /
• 1998

This study was focused on finding out the potential problems associated with organic farming system. The effect of composted animal manures subsequent inoculation of microbes on growth and yield of tomatoes (Lycopersicon esculentum Mill. 'Minicarol') were examined to develop a proper organic farming practice. Plant heights were greater in composted manure treatment than in conventional practice, whereas widths of leaves were higher in conventional field. Chlorophyll contents between various amount of composted manure application were gradually decreased and showed no significant differences after 45 days of planting. The yield in the treatment applied 12 ton of composted animal manure per 10a as pre-planting fertilizer and following microbial inoculation were only 50-60% compared to that of conventional farming. However, yield increased up to 80% when additional composts were applied to the treatment received 6 ton of composted animal manure per 10a in the middle of cultivation. Microbial inoculation followed by composted manure application induced rapid decrease of nitrogen content in soil. However, the density of microorganisms was significantly increased. Tomatoes produced through organic farming were clear in color, Further, soluble solid and acid content were increased. The highest level of acid and solids were observed in the treatment applied 12 ton of digested swine manure per 10a. Although nitrogen content including ammonium and nitrate rapidly increased after application of composts, these were significantly reduced approximately 4-5 weeks after planting. The level of phosphorus, potassium, magnesium and calcium showed gradual decrease compared to nitrogen.

• Applied Biological Chemistry
• /
• v.41 no.6
• /
• pp.457-464
• /
• 1998

For investigating the effect of amount of applied fertilizer with pig and chicken manures in the field, the number of soil bacteria were counted on the full strength conventional nutrient broth (NB) medium and its 100-fold dilution (DNB) medium. From the control soil samples without organic amendments, the number of bacteria on DNB medium was 5 to 10 times higher than that on NB medium. However, population density on NB medium was higher than on DNB medium from the treated soil with 60 and 120 Mg/㏊ for 3 years. Most of isolates from DNB plates did not grow on the NB plates. There were only grown on the 100-fold dilution medium, so it was called as DNB organisms. The DNB organisms were occupied as dominant group over 60% fo isolates in control soil. However, their occupation rates were rapidly decreased in the treatment soil with pig and chicken manures above 60 and 120 Mg per ha. These DNB organisms (oligotrophs) were significantly low population desities in the treatment soil with organic materials.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.3
• /
• pp.577-586
• /
• 2017

The aim of this study was to investigate the effects of fermented earthworm cast feed prepared with three complexed probiotics containing Bacillus subtilis separated from the earthworm casts, on egg production, egg quality and odor removal from poultry house. A total of 240 laying hens were divided into 4 treatments and completely randomized. Experimental groups were divided into control, 0.3 to 0.5% (fermented earthworm cast feed FECF3, FECF5) and 0.2% commercial probiotics (CP). The egg production rate was significantly higher in the FECF groups compared to the control group fed the normal feed, but there was no difference in the CP group. Haugh units, egg shell thickness, breaking strength, egg yolk index and albumen height were increased in FECF groups compared to control but not in CP group. The n-3 content of eggs was higher in the FECF groups than in the control group, while the n-6/n-3 ratio was lower and there was no difference from the CP group. Ammonia production was lower in the CP and FECF groups compared to the control group after 5 days storage at room temperature to poultry feces.

• Journal of the Korean Applied Science and Technology
• /
• v.35 no.3
• /
• pp.787-796
• /
• 2018

This study was conducted to investigate the effects of probiotic mixture on fecal ammonia, caecal microorganism, immune response, egg quality and production in layer under heat stress (HS).A total of four hundred 50 week olds Hy-Line Brown layers were randomly divided into four groups of 100 heads each: C (control, room temperature $25^{\circ}C$), HS (heat stress $33^{\circ}C$), PM (HS plus probiotic mixture 500, 750 mg/kg of diets). Egg production, egg quality, spleen weight, blood IgG and lymphocyte concentrations were increased in the PM group compared to the HS group, while mortality, the heterophil:lymphocyte (H:L) ratio, and corticosterone levelswere significantly decreased. Lactobacillus was increased in the PM group compared to the HS group, but E. coli, coliform bacteria and aerobic bacteria were significantly reduced. Fecal ammonia production was significantly increased in the HS group compared to the PM group. In conclusion, the results of this study that these mixed probiotics can reduce the heat damage of the summer laying hens and can be an effective nutritional strategy to reduce odor generation from feces, and to improve egg quality and laying production through immune response and caecal microbial balance.

• Korean Journal of Soil Science and Fertilizer
• /
• v.35 no.5
• /
• pp.306-313
• /
• 2002

The investigation was conducted to find out amounts and ratios of N, P and K fertilizers applied on summer Chinese cabbage in 58 farmers' fields of highland area. The application levels of N, $P_2O_5$, $K_2O$, livestock manure and lime fertilizers were 444, 188, 390, 9,920 and $2,160kg\;ha^{-1}$, respectively, for summer Chinese cabbage. The ratios of basal dressing were 48% in N, 46.6% in $K_2O$. The frequencies of top dressing both N and K were 1.7 times. The kinds of compound fertilizers were in the order of 11-10-10+3+0.3 > 11-6-6+4+13+17 > 12-9-11+3+0.3 in basal application and 18-0-18+0.3 > 13-0-13+1+0.3 > 18-0-15+0.3 in top dressing. From the surveyed results, we could estimate that total 4,347 tons of N, $P_2O_5$, and $K_2O$ fertilizers were over used for summer Chinese cabbage by farmers in highland.

• Korean Journal of Soil Science and Fertilizer
• /
• v.29 no.1
• /
• pp.44-52
• /
• 1996

Humic substances of 17 organic fertilizers available on the market were the objects of study. The list of ingredients for formulation of them comprised fish meal. bone meal, oil-cakes, brewer's grains, peat, sawdust, wood bark, zeolite, soil conditioner, live-stock droppings, amino acid fermentation byproduct, chaff, limestone and others. Humic and fulvic acids were isolated from those substances and given chemical and spectroscopic analyses. Nutritional values of the organic fertilizers showed big diversity. Humification of organic matter was incomplete for some of the fertilizers as indicated by a high C/N ratio. Extractable humic acid percentage was higher, in general, than that of fulvic acid. Also the relative content of humin increased with advanced humification. Total acidity was closely related to phenolic hydroxyl groups. Relationships between carboxyl and hydroxyl groups. and carboxyl and alcoholic hydroxyl groups were very significant. Ultraviolet and visible light absorption spectra of humic and fulvic acids were substantially similar. The types of humic acids were B. P, and Rp. Two humic acids of the 17 samples belonged to B type. 3 to P type and all the rest to Rp type.

• Korean Journal of Soil Science and Fertilizer
• /
• v.29 no.3
• /
• pp.303-311
• /
• 1996

The microflora changes of 10 water-controled treatments combined with livestock manures(pig, chicken) and bulking agents(sawdust, paper sludge) were investigated. The B/F values of the P-1 and C-1(65%, $H_2O$) treatments were 3571 and 5400 respectively, but those of the P-4 and C-4(50%, $H_2O$) treatments showed very low values, 667 and 334, respectively. The B/F values tended to increase with higher water content of the treatments. In the composting processes, the successions of microflora, adapting the compost environments, took place via fluctuating temperature. In the high temperature period, the numbers of mesophilic bacteria and fungi decreased, but that of the spore forming bacteria increased. However, the number of mesophilic bacteria inereased during the cold period. The B/F values of compost ranged 25-300, which indicates a decrease in the quantity of bacteria. The time required for the temperature of compost to reach $60^{\circ}C$ showed different patterns. There was no pathogenic microorganism in the treatments which reached a high temperature in a short period of time, but, in the treatments which reached a high temperature over a Long period of time, the pathgenic microorganism was not still alive.

• Korean Journal of Agricultural Science
• /
• v.37 no.1
• /
• pp.53-60
• /
• 2010

Two experiments were conducted to investigate the effect of probiotics on the malodor removal. In experiment 1, dietary effects (several malodorous gas concentration of excreta, dry matter metabolizability, and blood profiles) were determined using laying hens. A total of 30 Hy-line brown layers, 68-wk of age, were randomly allocated into 5 groups with 3 replicates of 2 birds each. The treatments were probiotics free, 0.2% and 0.4 % addition of mixed probiotics (Bacillus subtilis PNG-4 +Lactobacillus acidophilus LAS), and 0.2 and 0.4 % addition of single probiotics (Bacillus subtilis PNG-4). In experiment 2, the effects of mixing of probiotics into the excreta on the malodorous gas removal was investigated. There were three treatments (probiotics free, Bacillus subtilis PNG-4 +Lactobacillus acidophilus LAS, and Bacillus subtilis PNG-4) with three replicates. The malodorous gas concentrations were detected at 0, 3, 7 and 14 day of incubation. In experiment 1, ammonia concentration was significantly decreased by feeding mixed probiotics at 14th day of incubation. However, amines, hydrogen sulfide, ethylmercapthan, and methylmercapthan were not significantly affected by mixed probiotics. Dry matter metabolizability was significantly increased by feeding probiotics, but no significant differences between single and mixed probiotics. There was no significant differences in blood profiles. In experiment 2, mixing of probiotics into the excreta did not affect the concentration of ammonia, amines, hydrogen sulfide, ethylmercapthan, and methylmercapthan. Therefore, these experiments suggested that Bacillus subtilis PNG-4 + Lactobacillus acidophilus LAS supplementations could improve ammonia gas removal, and dry matter metabolizability in layers. Also, decrease of ammonia concentration was higher in mixed probiotics group compare to the single probiotics group. On the other hand, mixing of probiotics into the excreta appeared not to be a useful method.

• Korean Journal of Organic Agriculture
• /
• v.8 no.3
• /
• pp.131-146
• /
• 2000

This experiment was focused on finding out the potential problems in organic farming system, which is recently emphasized on the produce of horticultural crops due to the serious environmental deterioration. Thus, in this study, the effect of the application of organic farming materials in soil on plant growth and components of chinese cabbage and the soil Physicochemistry property were compared with conventional culture. The results were summarized as follows : 1. The effect of soil chemical properties after application of organic farming materials, The amounts of pH and O.,M. in N, P, K treated plot were few of change, To the contray, fermentation compost and microorganisms fermentation compost treated plot were some increase, Amount of P$_2$O$_{5}$ , Ca and increase in comparison with the N, P, K treated plot, Specially chicken manure+ microorganisms fermentation compost treated plot was the highest. 2. Change of soil microbial after application of organic farming materials, The number of bacteria and actinomycetes in N, P, K treated plot were appeared definite direction, to the fermentation compost and microorganisms fermentation compost treated plot showed the increased tendency, specially, chicken manure+ microorganisms fermentation compost treated plot was the highest, 3. The growth characteristic of chinese cabbage after application of organic farming materials was superior in order of chemical fertilizers plot> microorganisms fermentation compost plot> fermentation compost plot. It shows the NO$_3$$^{[-10]}$ accumulation in chinese cabbage was higher in the outer leaves than in the inner leavess. It was some lower in the microorganisms fermentation compost plot, it was the highest at chemical fertilizers plot , all inner and outer leaves.

• Journal of Mushroom
• /
• v.15 no.3
• /
• pp.150-154
• /
• 2017

A self-propelled turner was developed to produce mushroom compost at low costs and high efficiency, and the uniformity of compost produced by an excavator, compost mixer, and self-propelled turner was compared. The material of the compost was mixed with rice straw and chicken manure at a dry weight ratio of 3:1. After the final turning, internal temperature distribution, water content of the compost pile, ash ratio, and uniformity of the compost pile were examined. After the compost was completed, the water content was $69.9{\pm}0.54%$, $72.1{\pm}0.15%$, and $74.5{\pm}0.82%$, respectively. The length of rice straw was $47.5{\pm}15.4cm$, $24.9{\pm}10.1cm$, and $31.0{\pm}10.6cm$, respectively. The ash content in the dry weight of each compost was $25.0{\pm}6.2%$, $33.6{\pm}4.2%$, and $28.2{\pm}1.1%$, respectively. The deviation in the length of rice straw was affected by the linear velocity of the spinner in the compost mixer and the self-propelled turner, which were 21.5 m/sec and 9 m/sec, respectively. As a result, the most uniform mushroom compost was produced by the self-propelled turner.