• Korean Journal of Microbiology
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• v.34 no.3
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• pp.154-161
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• 1998

To investigate whether the introduced genetic marker is useful to detect the survivalship and activity of the natural bacteria under the stressed conditions, one Gram-negative isolate, KP964 was transformed to the luminous phenotype by transferring luxAB gene. Under the starvation-stress this luminous bacterial culturability (determined by colony-forming-units [CFU] on agar plate) decreased rapidly below the detection limit by 37 days, while its total cell number (determined by AODC) remained almost the same as its initial inocular size. At that time period, the viable cell number was estimated to be 1400 times higher than its CFU number. The bioiuminescence (determined by relative light units [RLU]) produced under the same condition was also monitored and found to decrease more rapidly than the culturability by 5-fold. Under the other stresses, e.g., osmotic shocks, acid shock, and exposure to toxic chemicals, this bacterial strain did not show the reliable correlation between CFU and RLU. These results might not suggest the direct estimation of bioiuminescence from the stressed bacteria be an index of both the survivalship and its activity. However, when the stressed bacterial cells were incubated under the favorable condition by relieving from the existing stress, the potential bioiuminescence (the lag periods before the increase of bioiuminescence, the increase rates of bioiuminescence, and the maximal levels of bioiuminescence) was shown to be highly dependent upon the strengths of the stresses exposed to the bacterial cells. Therefore, analysis of the potential bioiuminescence from the stressed bacteria revealed good relationships with survival as well as activity.

• Journal of Sericultural and Entomological Science
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• v.16 no.2
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• pp.99-109
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• 1974

These studies aimed to establishment of controlling measure of the bud blight of mulberry tree when intermediate cutting harvest in autumn. The results obtained as following. 1. Effect of chemicals a) Results of 1972 (1) Of the seventeen chemicals tested. according to in vitro, the mycelial growth of Gibberella lateritium f. sp. mori was highly inhibited by PTAB and Benlate. PTAB and Benlate were also highly effective under the conditions of pathogen inoculated to the piece of mulberry tree and fungicidal action of these chemicals were stronger than the others. (2) Topsin-M. organic fungicide and plant growth regulator were not effective to inhibition of mycelial growth. (3) The effectiveness of fungicides were reduced gradually as the time after treatment increased. PTAB and Benlate retained approximately 50 percent of the original activity even after 15 days. (t) Of the twelve chemicals tested in fold, Benlate combination of PTAB with Apion, Apion and PTAB were the most effective fungicides for the control of bud blight of mulberry tree. (5) There is a sigificant difference between the timing on spraying, but it is not greatly effective for the control of bud blight of mulberry tree. b) Results of 1975 (1) Of the ten chemicals tested, according to in vitro RH 893, combination of Benlate with TMTD and TMTD were the most effective to inhibition of mycelial growth, followed by Benlate and combination of Benlate with Topsin M. (2) Of the eleven chemicals tested, Benlate, combination of Benlate with Kinondu, combination of Benlate with Topsin-M and Topsin M were highly effective under the renditions of pathogen inoculated to the piece of mulberry tree. (3) Of the eleven chemical evaluation tests were conducted in 3 experiment fold of Seoul, Chuncheun and Pusan, Benlate, Topsin-M, Apion, TMTD. combination of Benlate with Topsin-M, combination of Benlate with TMTD, combination of Benlate with Apion were highly effective chemicals for the control of bud blight of mulberry tree. (4) There is no difference between the timing on the chemical spraying after intermediate cutting harvest mulberry tree in autumn. 2. Ecological Control. When the mulberry shoot were cut off one to two buds from the top of intermediate cutting from 15th to 30th on October, bud blight of mulberry tree was greatly reduced in next spring compared with the effective chemical spraying. 3. Economical analysis on the results of chemical and ecological tests. When the expenses of ecological control for bud blight of mulberry tree per one ha was 100. thats of chemical control was from 159 to 254 according to spraying machines.

• Journal of the Korean Wood Science and Technology
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• v.45 no.5
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• pp.599-612
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• 2017

Human hair (HH) is produced as a waste from beauty parlor and barbershop. HH-based adhesives were formulated with NaOH-hydrolyzed HH, $H_2SO_4$-hydrolyzed chicken blood (CB) and PF as a crosslinking agent. Physicochemical properties and retention rate against hot water of the adhesives were measured to investigate the potential of HH as a raw material of wood adhesives. HH was composed of keratin-type protein of 80% and over. Ash of less than 0.1% was contained in HH. Among the amino acids included in HH, glutamic acid showed the highest content, followed by cysteine, serine, arginine and threonine. Solid content of the adhesives ranged from 33.2% to 41.8% depending on hydrolysis conditions of HH and PF type. Viscosity at $25^{\circ}C$ ranged from 300 to $600mPa{\cdot}s$ resulting in a sprayable adhesive. Retention rate against hot water measured to evaluate the water resistance of adhesives was the highest in the cured resin formulated with 5% NaOH-hydrolyzed HH and 5% $H_2SO_4$-hydrolyzed CB. Meanwhile, the molar ratio of formaldehyde to phenol in PF did not have a significant impact on the retention rate of HH-based adhesives. When the retention rates of HH-based adhesives were compared to those of conventional wood adhesive resins used for the production of wood-based panels extensively, HH-based adhesives formulated with 30 wt% PF showed lower retention rate than commercial urea-formaldehyde resin. However, when PF content was increased to 35 wt%, the retention rate greatly increased and approached to that of commercial melamine-urea-formaldehyde resin. Except for the results mentioned above, the analysis of economic feasibility suggests that HH-based adhesives can be used for the production of wood-based panels if HH is hydrolyzed in proper conditions and then the HH-based adhesives are formulated by the HH hydrolyzates with 35 wt% PF.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.5
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• pp.847-856
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• 2016

Recently, with a high level of economic growth, rapid urbanization, population, environment and housing problems were accompanied in Korea. In particular, the traffic problem has become a serious social problem. As the current transportation policy has been carried out, concentrating on traffic flow, in 2015, death rate for pedestrians while walking (1,795 persons) is 38.8% compared to entire death rate in car accident (4,621 persons), so there is need to solve it. Although, crosswalk should make pedestrian cross it safely, it has been made on the basis of the width of road without exact standard for current width of the crosswalk and the location of stop line. Moreover, in the area around many campuses or commercial facilities, crosswalks are set with not considering pedestrian passage, but designed uniformly. Therefore, the purpose of this study is to estimate reasonable dimension of crosswalk considering pedestrian traffic and walking speed and it makes the accident rate lower in the crosswalk, which has a lot of problems including decisions of vehicle traffic signal time, lack of pedestrian's signal timing, pedestrian's crossing of long-distance. The following are the methodology of the study. Firstly, for crosswalk calculation of specifications, examination relating existing regulations and researches dealing with crosswalk, pedestrians and stop line is needed. After analyzing problems of current width of crosswalk and stop line, present the methodology to calculation of specifications and basing on these things, calculation of specifications for crosswalk will be decided. In conclusion, the calculation of specification and improvement of stop line for crosswalk laid out in this study are expected to be utilized as base data in case of establishing relevant safety facilities and standards.

• Journal of the Korean Society for Marine Environment & Energy
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• v.11 no.3
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• pp.150-159
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• 2008

The marine litter generated as by-products of human activities and economic development enters to the sea through rivers or stream Indirectly, and through sea-based activities directly. It is commonly comprised of materials that degrade very slowly, such as various plastic products, polystyrene, glass, rubber, metal, wood, derelict fishing nets, wire, rope and so on. Such litter is found in the water column and on the seafloor as well as coastal areas In the Northwest Pacific region. It causes injury or death of human and other living organisms and also accident or damage of the vessel. It is not only a problem of country but also regional and/or global problem because it is transported by currents and winds from one country to another. In this regard, Northwest Pacific Action Plan (NOWPAP) Marine Litter Activity (MALITA) project had been carried out during 2006-2007 biennium and NOWPAP Regional Action Plan on Marine Litter (RAP MALI) has been also continuously implemented in the 2008-2009 biennium as next phase step of MALITA. MERRAC, one of four Regional Activity Centres (RACs) of NOWPAP, has developed monitoring guidelines, sectoral guidelines, and brochures related to sea-based marine litter and port reception facilities and services through MALITA project. Based upon these output, MERRAC will continuously implement relevant activities of RAP MALI in order to help to establish and improve a regional mechanism to deal with the sea-based marine litter problem. This paper aims to introduce MERRAC activities under MALITA and RAP MALI, and to suggest several recommendations to reduce marine litter in the NOWPAP region.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.4
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• pp.63-68
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• 2018

The establishment of Ground Control Points (GCPs) in UAV-Photogrammetry is a working process that requires the most time and expenditure. Recently, the rapid developments of navigation sensors and communication technologies have enabled Unmanned Aerial Vehicles (UAVs) to conduct photogrammetric mapping without using GCP because of the availability of new methods such as RTK (Real Time Kinematic) and PPK (Post Processed Kinematic) technology. In this study, an experiment was conducted to evaluate the potential of RTK-UAV mapping with no GCPs compared to that of non RTK-UAV mapping. The positioning accuracy results produced by images obtained simultaneously from the two different types of UAVs were compared and analyzed. One was a RTK-UAV without GCPs and the other was a non RTK-UAV with different numbers of GCPs. The images were taken with a Canon IXUS 127 camera (focal length 4.3mm, pixel size $1.3{\mu}m$) at a flying height of approximately 160m, corresponding to a nominal GSD of approximately 4.7cm. As a result, the RMSE (planimetric/vertical) of positional accuracy according to the number of GCPs by the non-RTK method was 4.8cm/8.2cm with 5 GCPs, 5.4cm/10.3cm with 4 GCPs, and 6.2cm/12.0cm with 3 GCPs. In the case of non RTK-UAV photogrammetry with no GCP, the positioning accuracy was decreased greatly to approximately 112.9 cm and 204.6 cm in the horizontal and vertical coordinates, respectively. On the other hand, in the case of the RTK method with no ground control point, the errors in the planimetric and vertical position coordinates were reduced remarkably to 13.1cm and 15.7cm, respectively, compared to the non-RTK method. Overall, UAV photogrammetry supported by RTK-GPS technology, enabling precise positioning without a control point, is expected to be useful in the field of spatial information in the future.

• Tuberculosis and Respiratory Diseases
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• v.43 no.1
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• pp.30-37
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• 1996

Background: The extraction methods of DNA from clinical samples are the major obstacle to use the PCR(polymerase Chain Reaction) in routine labortary for early detection of M. tuberculosis. We tried to improve the extraction method of DNA from sputum for establishment of the PCR in routine labortary by reducing the possibility of cross contamination and performing it easily and safely. Methods: We used the $InstaGene^{TM}$ DNA extraction kit(BioRad Co.) using Chelex 100 ion exchange resin for preparation of DNA. We compared InstaGene method in 100 cases of sputum from proteinase K method which is known as the most commonly used method for DNA purification(Experiment 1). And we compared InstaGene method in 98 cases of sputum from Microwave method developed by a company in Korea(Experiment 2). In experiment 1,245bps of IS6110 were amplified and then 188bps were amplified by nested PCR. In experiment 2,536bps in primary PCR and 276bps in nested PCR were amplified and analysed by agarose gel electrophoresis and EtBr staining. Results: When we chose AFB smear, culture, or AFB smear and culture as a standard test, PCR had low specificity and positive predictive value in both experiments. The InstaGene method has higher value in sensitivity and negative predictive value significantly than proteinase K method. The InstaGene method and the Microwave methods were similar in sensitivity, specificity, positive predictive value and negative predictive value. Conclusion: Even though both methods had lower possibility of cross contamination, shorter time requirement, simplicity, and economic advantages than Proteinase K method, the InstaGene method was a little simpler than the Microwave method. Therefore, in terms of usefulness in clinical application, the Instagene method seems to be the most useful method in DNA extraction for detection of M. tuberculosis using PCR. The reliability of this method will be clarified by further studies with enough clinical samples.

• Journal of Bio-Environment Control
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• v.25 no.4
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• pp.277-282
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• 2016

This study was aimed to investigate the effect of air-circulation fans on air temperature and relative humidity in a single-span tomato greenhouse (W: 7m, L: 25m, H: 3.2m). According to standard of fan layout by ASAE (1997), a total of 10 fans were bilaterally arranged in 2 rows in the experimental greenhouse. The distributions of air temperature and relative humidity were measured from 6 pm to 8 am under different conditions, with and without fans. The measurement heights were 0.7m, 1.7m and 2.7m. Under the condition of "fans off", the spatial differences of air temperature and relative humidity between upper and lower sides were $1.7^{\circ}C$ and 10.8%, respectively. The operation of 10 fans showed their differences to $0.1^{\circ}C$ and 3.2%. The number of fans and installation direction were evaluated their performance on reducing the spatial variation of air temperature and relative humidity. The experimental layouts were 5 and fans in 2 rows (bilaterally) and 10 fans in the one (same) direction. Under the condition of "6 fans on" and "5 fans on", the spatial differences of air temperature and relative humidity between upper and lower side were $0.3^{\circ}C$, 3.4% and $0.3^{\circ}C$ and 4.0%. The operation of 10 fans in the one direction reduced their differences to $0.5^{\circ}C$ and 4.9%. The overall findings of this study showed that there was no significant differences under each condition. Therefore, this study suggested that it is more economic and effective to install five fans in 2 rows (bilaterally) in the greenhouse (W: 7m, L: 25m, H: 3.2m).

• The Korean Journal of Air & Space Law and Policy
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• v.29 no.2
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• pp.135-161
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• 2014

An unmanned aerial vehicle (UAV), commonly known as a drone and also referred to as an unpiloted aerial vehicle and a remotely piloted aircraft (RPA) by the International Civil Aviation Organization (ICAO), is an aircraft without a human pilot aboard. ICAO classify unmanned aircraft into two types under Circular 328 AN/190. Unmanned aircraft, which is the core of the development of the aviation industry. However, there are also elements of the legal dispute. Unmanned aircraft are manufactured in small size, it is possible to shoot a record peripheral routes stored in high-performance cameras and sensors without the consent of the citizens, there is a risk of invasion of privacy. In addition, the occurrence of the people of invasion of privacy is expected to use of civilian unmanned aircraft. If the exposure of private life that people did not want for unmanned aircraft has occurred, may occur liability to the operator of unmanned aircraft, this is a factor to be taken into account for the development of unmanned aircraft industry. In the United States, which is currently led by the unmanned aircraft industry, policy related to unmanned aircraft, invasion of privacy is under development, is preparing an efficient measures making. Unmanned aircraft special law has not been enforced. So there is a need for legal measures based on infringement of privacy by the unmanned aircraft. US was presented Privacy Protection Act of unmanned aircraft (draft). However Korea has many laws have been enacted, to enact a new law, but will be able to harm the legal stability, there is a need for the enactment of laws for public safety of life. Although in force Personal Information Protection Law, unmanned aerospace, when the invasion of privacy occurs, it is difficult to apply the Personal Information Protection Law. So, it was presented a privacy protection bill with infringement of privacy of unmanned aircraft in the reference US legislation and the Personal Information Protection Act.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.337-351
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• 2015

The articles, published in the Journal of Korean Forest Society from Volume 1 (1962) to Volume 102 (2013), were investigated for the research trend analysis about forest plants for special purposes, i.e., edible plants, medicinal plants, feed resources, landscape plants, fiber plants, industrial usage, bee plants, bioenergy/phytoremediation uses, dye materials, and rare/endangered/endemic plants. These research articles were classified again based on the contents of research into following categories - habitat environment, ecology, physiology, propagation, silviculture (including planting and tending), genetics and breeding, identification, pest and disease control, animal-related research, components analysis and extracts, vegetation survey, biotechnology, management, recreation and forest healing, and research review. Among the total 2,433 articles published, 611 (25.1%) were related to plants for special usage or purposes. The highest frequency (14.9%) in publications was found in the field of silviculture followed by physiology, propagation, identification, and genetics and breeding, respectively. On the bases of usage, edible plants showed higher frequency (26.5%) than others, followed by industrial purpose, bioenergy/phytoremediation usage, landscape plants, medicinal plants, and rare/endangered/endemic plants. Populus plant species was the most popular in research, showing 62 articles; and Castanea crenata 36; Pinus koraiensis 35; Robinia pseudoacacia 20; Ginko biloba 17, etc. Based on the survey and analysis, the following points are suggested: 1) improved evaluation of forest plants as non-wood resources, 2) expanding research topics on the basis of production, management, and utilization of non-wood forest resources, 3) management of database of forest plant information and encouragement needed to strengthen cooperative researches satisfying the needs of other industrial and scientific areas, and 4) encouraging to promote traditional knowledge based research on forest plants.