• Korean Journal of Environmental Biology
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• v.24 no.2 s.62
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• pp.102-111
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• 2006

The increased occurrence of hyperglycemia and oxidative stress in streptozotocin (STZ) induced type I diabetes has been implicated in the etiology and pathology of disease complication. STZ has known to be genotoxic in a variety of assays including tests for microbial mutagenesis and unscheduled DNA synthesis in rat kidney. Diabetes mellitus (DM) is a pathologic condition, resulting in severe metabolic imbalances and non-physiologic changes in many tissues. We examined the effect of gamma radiation and KWNP on preventing the development of insulin dependent diabetes mellitus using streptozotocin-induced Fisher 344 diabetic rats. The hematological values (red blood cell and white blood cell), serum biochemical constituents-alkaline phosphatase (ALP), total cholesterol, triglycerides and insulin-were checked and the organs (testis, spleen and kidney) were weighed. The gonad indices of the STZ treated groups were much lower than the value of the control group. But the gonad indices of the KWNP treated groups were higher than those of the treated groups. The ratio of the weight of kidney to the body weight of the STZ treated groups was higher than that of the control group. The value of the diabetic group treated with KWNP after irradiation (F group) was lower than the other STZ treated groups. The white blood cell and ALP values of the F group were lower than the other STZ groups, as well. The cholesterol and triglyceride values of all the KWNP treated groups were significantly lower than the other groups. A significant increase (about 10 times) of insulin was detected in the F group. The results of hematological assay showed the distinctive damage in the irradiated and STZ treated groups. The quantity of apoptotic cells in seminiferous tubule of testis confirmed a serious damage as assessed in the STZ treated groups. These experimental results have revealed that treatment of the products of KWNP after irradiation has the antidiabetic effect in the STZ-induced diabetic rats. But the F group showed higher recuperative power. These experimental results have revealed that treatment of the gamma irradiation and KWNP have the recovering effect in the STZ-induced diabetic rats.

• The Korean Journal of Pharmacology
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• v.30 no.1
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• pp.111-116
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• 1994

The role of $Ca^{2+}$/calmodulin-dependent protein kinase II in the increase of myofilament $Ca^{2+}$ sensitivity by agonist and GTP was investigated in rabbit mesenteric ${\alpha}-toxin$ permeabilized artery. $0.3{\mu}M\;Ca^{2+}$ increased myosin light chain phosphorylations monotonically. $10\;{\mu}M$ norepinephrine and $10\;{\mu}M$ GTP potentiated increase of myosin light chain phosphorylations by $0.3{\mu}M\;Ca^{2+}$, which reaches a peak at 5 min and gradually declines to the $Ca^{2+}$ alone level at 20 min. At the early phase (1 min), $10\;{\mu}M$ KN 62, the inhibitor of $Ca^{2+}$/calmodulin-dependent protein kinase II , decreased myosin light chain phosphorylation levels by $10\;{\mu}M$ norepinephrine and $10\;{\mu}M$ GTP in the presence of $0.3{\mu}M\;Ca^{2+}.\;However\;10\;{\mu}M$ KN-62 did not affect the myosin light chain phosphorylations by $10\;{\mu}M$ norepinephrine and $10\;{\mu}M$ GTP in the presence of $0.3{\mu}M\;Ca^{2+}$ at the peak (5 min) and plateau phases (20 min). From these results, the role of $Ca^{2+}$/calmodulin-dependent protein kinase II may be different depending on time, which may play a role in increase of myofilamint $Ca^{2+}$ sensitivity by norepinephrine and GTP resulting from increase of myosin light chain phosphorylations at the early phase. However, at plateau phase, $Ca^{2+}$/calmodulin-dependent protein kinase II may not be involved in the increase of myofilament $Ca^{2+}$ sensitivity by norepinephrine and GTP in rabbit mesenteric ${\alpha}-toxin$ permeabilized artery.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.181-186
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• 1988

This experiment was conducted to examine the $PGF{2{\alpha}}$-induced changes in concentrations of ovarian and pituitary hormones of Korean native goats. Each goats received two injections of $PGF{2{\alpha}}$ (5mg each ; 3 hours apart) on day 10 of the estrous cycle. Jugular venous blood samples were collected at 0, 3, 6, 12, 24, 48 and 72 hours postinjection for quantification of LH, FSH, prolactin, progesterone and estradiol-$17{\beta}$. The results were summarized as follows ; The blood serum concentration of progesterone was decreased from pretreatment level of $4.15{\pm}1.8ng/ml$ to $2.52{\pm}1.2ng/ml$ (about 60%) within 3 hours and to $0.81{\pm}0.3ng/ml$ at 12 hours of the $PGF{2{\alpha}}$ injection. After 12 hours, the concentrations of progesterone were less than 1.02ng/ml by 72 hours postinjection. The concentrations of estradiol-$17{\beta}$ following treatment increased (p < 0.05) over the 72 hours. Initial concentration of LH was $3.0{\pm}0.3{\mu}IU/ml$. After treatment with $PGF{2{\alpha}}$, concentrations of LH increased within 12 hours but declined 12 and 72 hours from $4.1{\mu}IU/ml$ to $2.5{\mu}IU/ml$. Prior to administration of $PGF{2{\alpha}}$, mean concentration of FSH was $3.5{\pm}0.5{\mu}IU/ml$. Concentrations of FSH declined over time in goats treated with $PGF{2{\alpha}}$ on day 10 postestrus. The mean prolactin concentrations in the blood serum after $PGF{2{\alpha}}$ treatment were not significantly different from those of the pretreatment. It is concluded that the initial increase in LH is dependent on a decrease in serum progesterone and differences in patterns of secretion of gonadotropins might be caused by differences in progesterone or progesterone-estradiol ratio when luteal regression is induced on day 10 of the estrous cycle.

• Journal of Nutrition and Health
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• v.14 no.2
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• pp.97-104
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• 1981

In order to study the changes of components and enzyme activities during Chungkookjang-Koji preparation, the Kojies were prepared with Bacillus Natto, Bacillus subtilis and traditional method. The temperature of Koji materials during Koji preparation was very different according to the experimental group. The content of ethyl alcohol, reducing sugar, amino nitrogen and water soluble nitrogen were changed by the Koji preparing stage and experimental group. Amylase and protease activities showed on irregular change on standing and their activities were not remarkably different among the groups and appeared weakly.

• The korean journal of orthodontics
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• v.28 no.4 s.69
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• pp.611-626
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• 1998

Cytokines are intercellular peptide mediators that regulate homeostasis and host defense reactions in living body. Of the diversity of cytokines in terms of biological accomplishment, interleukin $1-{\beta}$($IL-1{\beta}$) and tumor necrosis factor(TNF) are the most conspicuous cytokines with a wide variety of effects on cells involved in inflammatory and immune responses, and likely to be involved in the inflammatory pathogenesis of oral tissue as well. The present study was designed to explicate the role of $IL-1{\beta}$ on inflammatory revelation of oral tissues in mice biochemically. In the Induced arthritis by injection of 10${\mu}g$ LPS shown the relaese of 0.93 ${\mu}g$ $IL-1{\beta}$/joint with a peak at at 4-5 h. and diminished at 24t and the release of $TNF_{\alpha}$ of 1.25 ${\mu}g$/joint with a peak at 2-3h and diminished at 6h. After injection of th $IL-1{\beta}$ into the joint, the mumber of leucocytes proliferated with a peak at 4-5h and diminished at 36h and the loss of proteoglycan showed with maximum at 15-30h. After injection of $IL-1{\beta}$ into the oral tissue, cycloosygenase metabolites ($PGE_2$) accumulated in the oral tissue with dose dependant. These elucidated $IL-1{\beta}$ to be inflammatory mediator in the early phase of its pathogenesis. Intraoral injection of recombinant $IL-1{\beta}$ induced the proliferation of leukocytes in situ. $IL-1{\beta}$ took an pertinent part in the development of inflammation and the succession of cellular infiltration. The results exemplify that $IL-1{\beta}$ plays a significant role in mediating inflammatory response induced by LPS in oral tissue, the inflammatory response is regulated by $IL-1{\beta}$ at an acute phase of pathogenesis.

• Asian Journal of Turfgrass Science
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• v.26 no.1
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• pp.24-34
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• 2012

The study was carried out to investigate the effects of polymer, calcium, perlite and chitosan on the growth of perennial ryegrass (Lolium perenne L., PR) and to provide a basic information needed for their practical application when establishing garden, parks, athletic field and golf courses with these materials. A total of 24 treatment combinations were applied in the study. Treatments were made of water-swelling polymer (WSP), calcium, perlite and chitosan mixed in soil organic amendment (SOA). Germination rate, turfgrass coverage, turfgrass density and top growth were evaluated in PR under greenhouse conditions. Significant differences were observed for these growth characteristics among the treatments. Turfgrass density and plant height, evaluated on a weekly basis, varied with time after seeding. A proper mixing rate of WSP was considered to be lower 3% for the growth of PR with an exception of being below 6% for turfgrass density. Germination rate and early survival capacity were greatly influenced by calcium and chitosan among the elements of calcium, perlite, and chitosan. But there was little effect by perlite. Calcium and chitosan were most effective one for turfgrass density and coverage, respectively. Top leaf-growth was influenced by all three elements, but the greatest effect was highly linked with calcium. Chitosan was very effective in early germination and vertical leaf growth, as compared with the others. Future studies are required for measuring the effect of WSP, calcium, perlite and chitosan on the turf growth characteristics in root zone mixtures of sand+SOA before a practical field use.

• Korean Journal of Food Science and Technology
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• v.38 no.4
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• pp.567-570
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• 2006

This study was carried out to investigate the effect of antihypertensive peptides originating from marine proteins on ACE inhibitory activity and systolic blood pressure in spontaneously hypertensive rats (SHR). Sixteen male SHR (SHR/NCrj) weighing approximately 270 g were randomly divided into few experimental groups based on diet: C (control), A (anchovy), P (pollack) and M (mackerel). The final body weights of P and M groups were higher, than those of C and A groups, but difference was not significant. Average reference blood pressure (RBP) was 224 mmHg at 12 weeks old. Compared with RBP, final systolic blood pressure of the marine peptide oops after 28 days of feeding with anchovy, pollack and mackerel fractions by gavage was decreased by 9.0% (A), 10.2% (P) and 14.3% (M), respectively, but was not different in C. Especially, final blood pressure of M was lower by 32 mmHg than RBP. These results suggested that peptide originated from mackerel hydrolysate was considered to have an antihypertensive fraction as effective lowering of blood pressure in SHR.

• Journal of architectural history
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• v.10 no.4 s.28
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• pp.77-92
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• 2001

현대의 건축시기는 다원성의 시기 또는 복합성의 시기라고 불리운다. 모더니즘과 포스트모더니즘의 시기를 거치며 다양한 형태적 실험의 과정을 겪었고 수많은 이데올로기와 이론들로 무장된 논쟁의 역사도 갖게 되었다. 이러한 현대건축의 다양성은 건축디자인이라는 전문직의 사회에 기여하는 역할을 반추(反芻)함과 동시에 다변하는 인간사고의 역동성을 반영하고 있다. 건축관련 책자나 저널 등에서 흔히 발견할 수 있는 Neo-Classicism, Deconstructivism, Minimalism, High-Tech, New Moderns 등등의 건축양식들은 스타일을 지칭하는 언어라기보다는 건축적 사고를 지칭하는 언어라고 할 수 있다. 결국 건축디자인이라는 작업은 인간을 둘러 싼 건조환경의 생성에서 출발하는 것이므로 건축적 사고방식은 그 시기의 사회현상과 밀접한 관계를 맺게 된다. 20세기 말부터 불어닥친 디지털 문화의 사회변혁은 전 세계를 하나의 채널로 엮게 되었고, 이러한 영향들은 지역과 민족, 그리고 종교적인 요소들까지도 혼합하여 국경을 초월한 미래의 새로운 이상들을 꿈꾸게 되었다. 19세기 산업혁명과 함께 급속히 벨라진 인류문명의 진보는 필수적으로 세계화를 동반하는 것이고, 세계화와 지역성사이의 괴리를 통해 나타나는 문화적 비판과 충돌은 아직도 진행상태인 것이다. 세계화라는 통염은 문명의 보편성을 기반으로 하며 전통문화의 파괴가 뒤따를 수밖에 없는데, 현재와 같이 IT혁명을 통해 급속도로 빨라진 세계문화의 교류는 한 국가가 뿌리를 내리고 있는 문화적 유산이라는 토양을 포기하여야 할 단계에 와 있다고 논평할 정도이다. 여기서 건축은 문화적 상징으로 작용하게 되는데, 그것이 심미적이든 관습적이든 또는, 경제적이든 건축가는 해답의 제시라는 임무에 직면하게 된다. 20세기에 진행된 모더니즘의 건축은 구조와 공간에 대한 고전건축의 숙원을 고덕건축이 이룬 것처럼 15세기 르네상스 시대로부터 진행된 형태와 공간, 기능, 그리고 아름다움과 기술(technology) 또는 경제성 등에 대한 건축적 의문과 탐구를 일단락지었다고 할 수 있다. 이러한 모더니즘의 한복판에 Mies van der Rohe라는 거장이 깊은 발자국을 남기며 한 시대를 이루었고 그가 남긴 시대정신의 이념과 가치를 Miesianism이라고 부른다. 실용성과 도덕성을 근본으로 하는 Miesianism은 대중에 호소하는 미적 표현주의를 부정하고 지역정서를 중시하는 문맥적 접근방식을 경시함으로써 보편화라는 현상을 불러일으킨 국제주의 양식의 한 부류로 비판을 받아왔다. 즉, Miesianism의 단순하고 강렬한 외형적 요소는 그것이 내포하는 기술적 합리성이나 공간적 완결성을 무시한 채 전 세계에 영향을 미쳤고 동시에 지역적 저항을 받게 되었다. 시카고 및 전 세계의 Miesian들, 즉 Mies van der Rohe의 제자들이나 그로부터 영향을 받은 수많은 건축가들은 이러한 저항과 비판에 직면하게 되며 새로운 사고의 시대적 요구 앞에 고뇌하게 된다. 한국에서는 1978년 Mies van der Rohe의 제자인 김종성이 미국에서 서울로 돌아와 '서울건축컨설탄트'를 설립하며 본격적으로 Miesianism의 규범적 건축론을 설파하기 시작하였다. 이른바 시카고 국제주의학파의 건축전수라고 할 수 있는데 '서울건축컨설탄트'를 통하여 배출된 김종성의 제자들은 명쾌하고도 간결한 건축해법의 경험을 토대름대로의 정체성을 갖고자 노력하였으나 결국 다원적 가치를 요구하는 시대적 흐름 속에 혼란을 겪고 있는 것 또한 사실이다. 본 연구는 Miesianism의 기원을 밝히고 그것의 실수와 오류를 밝힘과 동시에 현대의 여러 가지 건축유형들과 비교하여 봄으로써 Post-Miesianism의 실체와 그 미래에 대하여 고찰하여 보고자 한다. 미스의 유산이라고 할 수 있는 Miesianism의 요체는 첫째, Schinkel로부터 이어받은 시대정신의 사명감, 둘째, Berlage가 전해준 전문가로서의 도덕성을 바탕으로 하여 이루어진 기술적 합리성의 실현, 그리고 마지막으로 그 당시의 추상예술에 대한 이해로써 받아들인 nee-Suprematism의 아방 가르데적인 자세라고 할 수 있다. 그러나 이것들을 전파하고 수용하는 과정에서 미스적인 단순하고 명쾌한 외관이 더욱 강한 인상을 남기게 되었고 또한 미스가 현대성의 본질이라고 믿는 기술의 전수는 모든 외관을 동일하게 만드는 International Style로서의 보편적 획일성을 초래하였다. Mies van der Rohe의 강력하고 규범적인 dogma는 그의 제자들에게는 강렬한 카리스마로 각인되었고, Mies가 원한 것이 아닐지라도 그들을 하나의 틀 속에 가두는 영향력을 행사하였다. 2세대이든 3세대이든 기술적 합리성과 추상적 단순미라는 믿음을 맹목적으로 추종할 때 그들은 Miesian으로 남아있게 되며, 거기서 벗어났을 때 non-Miesian, 또는 배신자로 취급하기까지 하였다. 이것은 미스의 제자들에게 강한 족쇄가 되어 형태적 또는 개념적 변화의 시도를 어렵게 하였고 시대적 요구에 부응하지 못한다는 평가에 이르게 되었다. 문화의 다양성과 역동성을 인정함과 동시에 Miesianism에 대한 새롭고 시대에 적합한 해석을 기대하게 되는데, 이러한 기대 속에서 우리는 Post-Miesianism이라는 새로운 유형을 엿볼 수 있게 된다. Post-Miesianism의 징후를 현대의 다양한 건축유형에서 발견할 수 있는데, High-Tech의 구축적이고 정밀한 건축 기술적 해법과 Minimalism의 반복성을 통한 추상미의 표현 등에서 뿐만이 아니라 Post-Modernism의 인간성의 추구나 문맥적 고려, 또는 해체주의의 형이상학적이고 아방가르데적인 실험주의 정신까지조차 Post-Miesianism이 공유할 수 있는 부분이 있다고 보여진다. 또한, 미스의 영향이 2세대, 3세대의 건축가들에게 미치면서 여러 방향으로 변질되어 가는 것을 우리는 발견할 수 있으며 그러한 시대성에 적응하는 시도들이야말로 새로운 미래를 향한 Post-Miesianism의 실체인 것이다. Mies가 말하였듯이 한 시대의 끝은 그 시대가 완전히 이해되었을 때이며, 모더니즘의 숙제는 아직 해결되지 않았으므로 Miesianism의 탐구는 Post-Miesianism이라는 새로운 주제로 계속될 것이다. 이를 통하여 21세기 디지털 문명의 시대 속에서 현대건축이 안을 수밖에 없는 보편성과 지역성의 충돌이라는 문제의 해결에 한 발걸음 다가갈 수 있기를 기대하여 본다.

• Korean Journal of Poultry Science
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• v.19 no.4
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• pp.217-225
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• 1992

This research was carried out to determine the 25-Hydroxyvitamin $D_3$[25(OH)$D_3$] content in liver of broiler Hubbard chicks fed vitamin VD-deficient diet for 31 days in a subdued light room and exposed to UVB light (maximum intensity at 297nm) with dose of 0.204 or 0.408 mJ/$\textrm{cm}^2$(30 or 60 min irradiation) . The lipid in liver collected at 0~138 hr after irradiation was extracted by chloroform-methanol(2：1, v /v) and 25(OH)$D_3$ fraction was separated by Sep-Pak silica cartridge. The 25(OH)$D_3$ concentration was measured by normal phase HPLC. The negative control chicks Presented 25(OH)D$_3$17.5 ng/g liver. When 0.204mJ/$\textrm{cm}^2$ was treated to whole body of chicks, the 25(OH)$D_3$ level was increased to 37.8 ng/g at 12 hr after irradiation, the peak concentration, 40.5 ng /g was appeared at the time of 86 hr, and decreasing trend was shown thereafter until 138 hr, the final time in this study. When 0.408 mJ/$\textrm{cm}^2$ was applied, the 25(OH)$D_3$ content was 36.7 ng /g liver at 12 hr, 61.4 ng/g(maximum value ) was appeared at 42 hr, and 39.5 ng /g at 138 hr. The increased absolute amounts in liver 25(OH)$D_3$ were 23 and 43.9 ng/g as chicks were exposed to UVB light with dose of 0.204 and 0.408mJ/$\textrm{cm}^2$, respectively. Consequently, it was found that when double dose of UVB light was irradiated to the chicks, their liver samples produced nearly double 25(OH)$D_3$ at 42 hr after exposure, and the peak value was presented earlier by 24 hr than that in the low dose treatment.

• Journal of Food Hygiene and Safety
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• v.17 no.1
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• pp.1-7
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• 2002

This study was performed to investigate the possibility of water purification by a wild train of Oenanthe javanica DC. Three commercially available dishwashing detergents and a standard surfactant, linear alkylbenzene sulfonate (LAS), were used for this study. The experiment was done in 1.5 ι transluscent aquariums. The plants were distributed into various concentrations of detergents and various kinds of detergent in the separate aquariums. The wet weight of the plants was significantly decreased (p<0.05), and the visual vitality of the plants also decreased in 2 days. The higher the concentration of detergent was, and the more time the plants were exposed to the detergents, the more decrease of growth was observed. The pH value of the culture media decreased in 2 days and in 4 days, then slightly increased in 6 days. However, the pH value of the media did not return to the initial neutral level of pH in 6 days. The pH value of the culture media containing the LAS remarkably increased in 6 days and increased to a neutral pH value in 18 days (p<0.01) as the pH of the other culture media. The chemical oxygen demand (COD) of the culture media gradually increased over the 4 days. A decrease of COD was observed in 6 days, but no tendency was observed between 12 and 18 days. The detergent in the culture media was highly significantly decreased in 2 days (p<0.01) and gradually decreased after this. After 6 days the remaining detergent was 12.4∼23.7% from the various levels of initially added concentration, and 22.4 ∼34.2% from the flour kinds of detergents. These results show that the reduction of detergent was caused by Oenanthe javanica and the effect was significant during the first 6 days when the plants were still growing well. These results indicate that the plant purifies contaminated water for several days and the effect could be variable according to the level of contamination and the environment in which the plant grows.