• The Journal of Society for e-Business Studies
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• v.24 no.3
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• pp.53-71
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• 2019

As software systems become more complex and larger, software systems require a way to reuse software components or modules to provide new functionality. This paper designed a development method of web system combining SOA(Service Oriented Architecture) with MPSL(Multi-Software Product Line). According to provides SOA and MPSL, this paper suggested to service providers and service users to provide and reuse variable services. From the viewpoint of service provider, the suggested method identifies and implements reusable variable services as features by syntax-based, functional-based, and behavior-based methods applying feature identification guidelines and manages them as reuse assets. From the user's point of view, it is possible to develop a web system by constructing a service by workflow model as a method of structure and reconfigure services. As a result of measuring the reuse of the web system constructed in this paper by the function point, the cost reduction effect was verified by applying it to the similar project with the increase of reuse.

• Proceedings of the KSRS Conference
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• 2007.03a
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• pp.82-87
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• 2007

웹 GIS 서비스는 웹의 구현기술을 기존의 GIS와 결합하여 Internet 또는 Intranet 환경에서 지리정보의 입력，수정，조작， 분석，출력 등의 작업을 처리하여 네트워크 환경에서 서비스를 제공할 수 있도록 구축된 시스템을 말한다. 웹 GIS 서비스의 구현은 단순히 지리공간 자료를 보여주는 일반적인 형태의 응용에서부터 공간 분석을 수행하여 의사결정에 도움을 줄수 있는 응용시스템까지 다양한 구현이 가능하다. 웹 GIS 서비스는 많은 GIS 표준 명세 규칙인 필수 사항과 옵션 사항으로 구분되어 있으며 종종 동일한 표준을 지원한다 할지라도 상호 연동을 위한 인터페이스를 지원하지 않아 서비스의 확장이 어렵다. 본 연구는 이러한 단점을 보완하기 위하여 향후의 웹 GIS 응용 서버 확장에 필요한 국제표준 및 기술을 고려하여 웹 서비스를 구성하여 이들을 재사용 재조합 하기 위한 인터페이스를 구축하였다. 또한 이를 기반으로 Ajax 기법이 적용된 비동기식 호출을 사용하여 Client 환경에서 최적의성능을 발휘하는 방안을 소개하였다.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.11a
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• pp.684-687
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• 2013

빅데이터 환경은 수많은 데이터의 조합으로 가치를 발견하여 이를 활용하는 것이다. 이러한 환경의 전제조건은 데이터의 공개 및 공유 개방이 될 것이다. 하지만 데이터 공개 시 개인정보와 같은 정보가 포함되어 법적 도덕적인 문제나 공개된 정보의 범죄 활용 등 2차적인 피해가 발생할 수 있어 데이터 공개 시 개인정보에 대한 익명화가 반드시 필요하다. 하지만 익명화된 데이터는 다른 정보와 결합을 통하여 재식별되어 비익명화 될 가능성이 항상 존재한다. 따라서 본 논문에서는 데이터 공개 시 익명화된 데이터를 공개하기 전에 재식별성에 대한 위험을 평가하는 테스트 방법론을 제안한다. 제안하는 방법론은 실제 테스트를 수행하는 3가지 과정 및 테스트 레벨 설정과 익명화 시 고려해야 할 부분으로 이루어져 있다. 제안하는 방법론을 통하여 안전한 데이터 공개 환경이 조성되어 빅데이터 시대에 개인정보에 안전한 데이터 공유와 개방이 이루어질 것으로 기대한다.

• Journal of the Korea Concrete Institute
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• v.18 no.2 s.92
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• pp.205-211
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• 2006

The objective of this study was to evaluate the durability of low shrinkage high performance concrete(LSHPC), which was combined with expansive additives and shrinkage reducing admixtures. We tested for not only LSHPC but also high performance concrete(HPC) and normal concrete(NC) to be compared with the durability of LSHPC. HPC was made in the same water-binder ratio of LSHPC without expansive additives and shrinkage reducing admixture. As a result, it was found that LSHPC had higher compressive and tensile strength than that of HPC. LSHPC showed more excellent performance than HPC and NC in the case of resistance to chloride ion penetration and resistance to carbonation and also showed nearly 100 durability factor in the freeze-thawing test with 500 cycles. From the examination about the watertightness and the pore distribution, it was found that the durability of LSHPC was improved because its hardened cement paste is organized closer. So we can conclude that when LSHPC is applied to structures in field, it is possible to reduce the shrinkage and crack in concrete and improve the durability.

• Journal of Life Science
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• v.19 no.2
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• pp.284-288
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• 2009

The innate immune system is important for the first line of host defence against infectious agents, which have penetrated the mechanical barriers. Mannose-binding lectin (MBL or mannan-binding protein, MBP) is a serum protein that is synthesized in the liver as a part of the acute phase response. MBL binds to carbohydrate structures presented by a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL is synthesized as a monomer that has a carboxy-terminal carbohydrate recognition domain, a neck region and a collagen region. Low MBL level was reported to be the most frequent immuno-deficiency syndrome. Although extensive studies have yielded detailed information on the structure of MBL, functions of the MBL complex are not fully understood yet. We, here, present cloning process of MBL cDNA from the rat liver and production of truncated recombinant MBL protein using a bacterial expression system in order to produce anti-MBL polyclonal antibody. Anti-MBL polyclonal antibody was raised in a New Zealand rabbit and its affinity was tested against recombinant protein using western blot technique. MBL cDNA, recombinant protein and anti-MBL antibody could be used as great arsenals to dissect cellular biochemistry of MBL.

• Journal of Life Science
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• v.28 no.1
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• pp.78-82
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• 2018

Detection of pathogenic microorganisms takes several days by conventional methods. It is necessary to assess microorganisms in a timely manner to reduce the risk of spreading infection. For this purpose, bacteriophages are chosen for use as a biosensing tool due to their host specificity, wide abundance, and safety. However, their lytic cycle limits their efficacy as biosensors. Phage proteins involved in binding to bacteria could be a robust alternative in resolving this drawback. Here, a fragment of tail protein J (residues 784 to 1,132) of phage lambda fused with 6X His-tag (6HN-J) at its N-terminus was cloned, overexpressed, purified, and characterized for its binding with microorganisms. The purified protein demonstrated a size of about 38 kDa in sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) and bound with anti-His monoclonal antibodies. It bound specifically to Escherichia coli K-12, and not Salmonella typhimurium, Bacillus subtilis, or Pseudomonas aeruginosa in dot blotting. Binding of the protein to E. coli K-12 inhibited about 50% of the in vivo adsorption of the phage lambda to host cells at a concentration of $1{\mu}g/ml$ 6HN-J protein and almost 100% at $25{\mu}g/ml$ 6HN-J. The results suggest that a fusion viral protein could be utilized as a biosensing element (e.g., protein chips) for detecting microorganisms in real time.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.38 no.8
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• pp.798-805
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• 2010

Whole composite structure small class (150kg) satellite, STSAT-3, was initially developed in Korea. The structure does have aluminum frames that support the structure, and it is composed of only composite sandwich panels. A number of electronic boxes and mechanical apparatus will be shielded within the compartments built up by the composite panels. This study focused on the random vibration responses of the satellite. For this objective, vibration tests and analyses have been successfully performed with respect to STM (structure and thermal model) of the satellite. Additionally, through the experiment and theoretical analyses, the both results' accuracy was verified by comparison each other.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.231-234
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• 2001

We performed a basic experiment for rapid. on-line, real-time measurement of HBsAg by using a BIACORE biosensor, a chip-based sensor utilizing surface plasmon resonance technology to quantify the recognition and interaction of biomolecules. We immobilized an a -HBsAg antibody on a CM5 chip surface which was activated by N-hydroxysuccinimide for amine coupling with HBsAg, and measured the mass increase from the coupling. This study showed the potential of this biosensor-based method as a rapid, multi-sample, on-line assay. Once properly validated, it can serve as a more powerful method for HBsAg quantification.

• KSBB Journal
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• v.21 no.3
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• pp.204-211
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• 2006

For the production of the recombinant human interferon-gamma(rhIFN-${\gamma}$) in Escherichia coli, human glucagon and ferritin heavy chain were used as fusion partners. Even though rhIFN-${\gamma}$ is expressed as an inclusion body form in E. coli because of strong hydrophobicity of itself, over 50% of fused rhIFN-${\gamma}$ was expressed as soluble form in E. coli $Origami^{TM}$(DE3) harboring pT7FH(HE)-IFN-${\gamma}$ which encodes ferritin heavy chain-fused rhIFN-${\gamma}$. In the case of using glucagon-ferritin heavy chain hybrid mutant as a fusion partner, 6X His-tag was additionally introduced to N-terminus of GFHM(HE)-IFN-${\gamma}$ for enhancing purification yields of rhIFN-${\gamma}$. Fusion protein HGFHM(HE)-IFN-${\gamma}$ with two 6X His-tag was more effectively bound to Ni-NTA agarose bead than GFHM(HE)-IFN-${\gamma}$ with a 6X His-tag. rhIFN-${\gamma}$ was completely purified from enterokinase-treated HGFHM(HE)-IFN-${\gamma}$ by Ni-NTA affinity column. For high-level production of rhIFN-${\gamma}$, glucose was used as the sole carbon source with simple exponential feeding rate($2.4{\sim}7.2g/h$) in fed-batch process. The effective lactose concentration for the expression of the rhIFN-${\gamma}$ was $10{\sim}20mM$. Under the fed-batch culture conditions, rhIFN-${\gamma}$ production yield reached 11 g DCW/L for 6 hours after lactose induction.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.8
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• pp.547-553
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• 2013

This paper investigates the effect of coal-fired fly ash on dry $CO_2$ sorbents as the supports and additives. For this purpose, various kinds of dry sorbent were manufactured by mixing fly-ash, the primary $CO_2$ absorption components (NaOH and CaO) and water with their different combination. Thereafter, their $CO_2$ absorption performance and the property were analyzed. As a result, variation of absorption efficiency and temperature as well as $CO_2$ desorption of the sorbents are confirmed, which may be primarily ascribed to fly-ash addition to the sorbents. Particularly, fly-ash effect is strongly measured in the sorbent manufactured by mixing all four components (named WNCF sorbents). Absorption efficiency of WNCF sorbents at $550^{\circ}C$ is 35.6% higher than that of flyash free sorbent and desorption is solely observed in WNCF sorbents. Fly-ash in WNCF sorbents leads to increase the dispersity of $CO_2$ absorption components and decrease their particle size in the sorbents. In addition, fly-ash is used as the supports and pozzolanic reaction is hindered by NaOH in WNCF sorbent. Furthermore, $CO_2$ desorption from the sorbents may be due to fly-ash. The interaction between fly-ash and $CO_2$ absorption components substantially attenuate the strength between captured $CO_2$ in CaO and NaOH.