• Korean Journal of Poultry Science
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• v.30 no.1
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• pp.61-66
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• 2003

This study was conducted to investigate the effects of dietary germanium biotite supplementation on egg quality and fecal noxious gas content in laying hens. One hundred and forty-four 40-wk-old, ISA Brown layers were used in this experiment. Dietary treatments were 1) CON(control diet), 2) GB0.5(control diet + 0.5% germanium biotite), 3) GB1.0 (control diet + 1.0% germanium biotite) and 4) GB1.5(control diet + 1.5% germanium biotite). Hen-day egg Production and egg shell breaking strength were not influenced by germanium biotite supplementation. However, egg weight decrease as the level of germanium biotite supplementation increased in the diets increased(Cubic effect, p<0.02). Eggshell thickness, yolk color and yolk index were not influenced by garmanium biotite supplementation. Serum triglyceride concentration as dietary germanium biotite increased (linear effect P<0.02; quadratic effect, p<0.05). Dietary supplementation of germanium biotite reduced fecal NH$_3$-N concentration (P<0.01), propionic acid(P<0.01), butyric acid( P<0.05) and acetic aicd(P<0.01) concentrations in the feces. In conclusion, the results of this experiment indicated that dietary germanium biotite supplementation did not affect egg shell quality, but reduced fecal NH$_3$-N concentration.

• Korean Journal of Poultry Science
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• v.30 no.1
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• pp.67-72
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• 2003

This study was conducted to investigate the effect of germanium biotite on the growth performance, blood composition , fecal NH$_3$-N and volatile fatty acid(VFA) in broiler chicks. Two hundred forty broiler chicks were randomly allocated into five treatments with four replicates for five weeks. Dietary treatments included 1) Control, 2) GB1(basal diet + 200 mesh 0.5%), 3) GB2(basal diet + 325 mesh 0.5%), 4) GB3(basal diet + 200 mesh 1.0%) and 5) GB4(basal diet +325 nesh 1.0%) For overall period, weight gain, feed intake and feed conversion were not significantly different among treatments (P>0.05). Digestibilities of nutrients were not affected by adding GB(P>0.05). No differences occurred in red blood cell(RBC), white blood cell(WBC), hematocrit(HCT), hemoglobin(Hb), lymphocyte, monocyte and platelet of blood among the treatments (P>0.05). Fecal NH$_3$-N concentration of chicks fed GB1.0 diets(GB3 and GB4) was lower(P<0.03) than chicks fed GB0.5 diets(GB1 and GB2). Propionic acid and butyric acid in feces of chicks fed GB diets were lower(P<0.04) than in chicks fed control diet. GB diets reduced fecal acetic acid significantly compared to control (P<0.01). Also, acetic acid of feces in chicks fed GB1.0 diets(GB3 and GB4) was lower(P<0.02) than GB 0.5 diets(GB1 and GB2) treatments. In conclusion, dietary germanium biotite was an effective means of decreasing fecal NH$_3$-N and volatile fatty acid(VFA) emission.

• KOREAN POULTRY JOURNAL
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• v.38 no.6 s.440
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• pp.120-123
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• 2006

선모자연농원(대표 양승덕)은 지난 2006년 3월 10일 우리 시대에 맞는 친환경 생산물을 이용해 무항생제, 항산화제로 소비자에게 깨끗하고 신선한 계란을 공급하고자 3년간의 지속적인 연구 끝에 흑운모와 녹차를 함유한 유정란의 품질인증을 받았고, 현재 특허등록을 위해 출원한 상태이다.(출원번호 40-2006-0012500). 양승덕 사장은 흑운모가 황토와 맥반석보다 약 3배 이상 원적외선 방사율(94%)이 높으며 게르마늄 함유량이 36ppm에 이르는 생명의 돌로서 예전부터 흑운모석을 약돌이라 부른 것에 착안하여 산란계에도 흑운모 분말을 배합한 사료를 급여하였다. 본고는 웰빙시대에 걸 맞는 흑운모, 녹차의 기능과 이를 이용해 국내에서 어렵게 생산한 "흑운모 · 녹차유정란"의 특성을 살려 생산하는 현장을 취재하였다.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.787-796
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• 2003

Two experiments were conducted to investigate the effect of germanium biotite (GB) on growth performance, nutrient digestibility and fecal gas emission in pigs. In Exp 1., a total of one hundred nursery pigs (initial body weight 13.12${\pm}$0.15kg) were used in a 21 d growth assay. The five treatments were control (CON, basal diet), GB0.5-200 (basal diet + 0.5% GB, 200mesh), GB1.0-200 (basal diet + 1.0% GB, 200mesh), GB0.5-325 (basal diet + 0.5% GB, 325mesh), GB1.0-325 (basal diet + 1.0% GB, 325mesh). Each treatment had four replicates with five pigs per replicate. ADG, ADFI and gain/feed were not significantly different among the treatments. Fecal NH3-N concentration of pigs fed the GB325 diet was lower than that of pigs fed the GB200 diet (P=0.01). The GB treatments reduced fecal volatile fatty acids significantly compared to the CON (propionic acid, P=0.01; butyric acid, P=0.01; acetic acid, P=0.02). Especially, fecal propionic acid concentration of pigs fed the GB325 diets was lower than that of pigs fed the GB200 diets (P=0.02). In Exp 2., a total of seventy five pigs (initial body weight 21.18${\pm}$0.15kg) were used in a 28 d growth assay. The treatments were same as described for Exp. 1. Each treatment had five replicates with three pigs per replicate. The GB1.0 treatments significantly increased the ADG compared to the GB0.5 treatments (P=0.03). The DM and N digestibility of pigs fed the GB1.0 diets were higher than that for pigs fed the GB0.5 diets (P=0.01). Also, the Ca digestibility of pigs fed the GB diets was higher than that for pigs fed the CON diets (P=0.01). The fecal NH3-N concentrations for the GB treatments were lower than that for the CON (P=0.01). The GB325 treatments significantly decreased the fecal NH3-N concentration compared to the GB200 treatments (P=0.03). The fecal butyric acid concentration for the GB325 treatments was lower than that for the GB200 treatment (P=0.04). In conclusion, the results obtained from these feeding trials suggest that the dietary GB for nursery pigs affects fecal noxious gas emission. In growing pigs, dietary GB was effective to improve ADG and decrease fecal noxious gas emission.

• Feed Journal
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• v.2 no.3
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• pp.126-129
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• 2004

• Korean Journal of Poultry Science
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• v.30 no.4
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• pp.275-280
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• 2003

This study conducted to investigate the effect of dietary germanium biotite by protein level in laying hen diets. One hundred forty four, 51 weeks old ISA brown commercial layer, were used in experiment. Dietary treatments were 1) low protein diet(LPD), 2) high protein diet(HPD), 3) LPD-GB(LPD + 1.0% germanium biotite) and 4) HPD-GB(HPD + 1.0% germanium biotite). Henday egg production tended to be increased as the concentration of protein in diets increased with significant difference(P<0.01). Egg weight tended to decrease by increasing of supplementation germanium biotite in the diets(P<0.01). Egg shell breaking strength was not influenced by germanium biotite supplementation(P>0.05). Large band of egg decrease as increasing of supplementation germanium biotite in the diets(P<0.02). Sharp and middle band of egg were not influenced by germanium biotite supplementation. Egg yolk index tended to decrease as increasing of supplementation germanium biotite in the diets(p<0.01). Fecal propionic acid(P<0.01) and butyric acid(P<0.03) were decrease as the concentration of germanium biotite in the diet was increased. Also, butyric acid increased as the concentration of protein in diets increased with significant difference(P<0.02). Supplementation germanium biotite in the diet reduced the fecal acetic acid(P<0.01). Fecal $NH_3$-N of hens fed HPD-GB diet was decreased(P<0.05) compared to that LPD-GB diet. In conclusion, germanium biotite supplementation to layer diets can reduce fecal volatile fatty acid compabebts.

• Korean Journal of Veterinary Research
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• v.52 no.1
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• pp.33-38
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• 2012

Germanium biotite, a natural mineral, has been used as a feed supplement to reinforce innate immune ability. The aim of the present study was to evaluate the effects of germanium biotite on the adsorptive and inhibition of growth abilities against Escherichia (E.) coli and Salmonella spp. in vitro. Two strains of enterotoxigenic E. coli and four strains of two Salmonella serotypes (Salmonella Derby and Salmonella Typhimurium), major bacterial diarrheal pathogens, were used for this experiment. The absorptive ability of germanium biotite against most Salmonella used in present experiment was observed weakly. The germanium biotite, however, showed significant effect of bacterial growth inhibition in most experiment bacteria. These results suggest that the use of the germanium biotite as feed supplement could alleviate diarrhea following inhibition of bacteria growth. It is also presumed that antibiotics usage for farm animals, considered as causes of antibiotic residue in meat and emerging antibiotic resistance, could be reduced through the use of germanium biotite as a feed supplement, in place of antibiotics used for the prevention of diarrhea.