• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.18 no.6
• /
• pp.550-556
• /
• 1985

In order to evaluate the marine mollusc muscle as foodstuff not only from the biochemical aspect but also from the view point of food science, we have analyzed the protein and amino acid compositions of the echiurid (Urechis unicinctus) and sea hare (Aplysia kurodai) muscle. The protein quality of the muscles was also investigated using in vitro methods based on in vitro digestibility, predicted digestibility, computed PER (C-PER) and discriminant computed PER(DC-PER). The remarkable feature of the protein compositions of the both muscles was that water soluble protein occupied a large amount of the muscle protein with fairly lower contents of the salt soluble protein. From the analysis of SDS-PAG electrophoresis, the sarcoplasmic proteins in the echiurid and the sea hare muscles were composed of 15 and 10 subunits, respectively. The free amino acid compositions of the total amino acids in the echiurid and sea hare muscle were characterized with $75\%$ of glycine and alanine, and with $78\%$ of taurine, respectively. The amino acid anaylsis of both muscle proteins showed that the echiurid muscle was rich in glycine, aspartic acid, glutamic acid, arginine and lysine, but was poor in cysteine, while the sea hare muscle was rich in glycine, glutamic acid, aspartic acid and arginine, but was negligible in cysteine and tryptophan. In the total amino acid profiles of the freeze dried muscles in echiurid and sea hare, there was not found a significant difference compared to the amino acid compositions of the muscle proteins. Predicting the protein quality of the echiurid and sea hare muscles using the in vitro method, it was apparently low compared to the muscle protein of fishes.

• Progress in Medical Physics
• /
• v.18 no.2
• /
• pp.73-80
• /
• 2007

Fruitful findings have been produced from five out of sixty cells which were obtained from each 63 individual Aplisia caught at the Jeju coast. Spiking patterns of three out of five cells, such as relaxation oscillator, bursting within a short time of the inter-burst interval, chaotic bursting, period doubling sequences, bursting with long trains of action potentials separated by short silent periods, regular repeated beating or elliptic bursting, and silent states had been changed in order as the temperature was lowered to $10^{\circ}C\;from\;32^{\circ}C$. In the intervals of every about 40 minutes repeated ups and downs of temperature produced similar firing patterns at the allowable temperature ranges. The other two cells showed difference from these. The amplitudes of the action potentials of the two cells will not be highly decreased in 24 hours. Average spike frequencies, the inter-burst interval, peak to peak spike amplitude of action potentials, minimum potential values are compared and analyzed by using the computer programme. The spike frequencies according to temperature show the distribution of bell type, with maximal spike frequencies at intermediate temperatures and minimal ones at either end. The most common pattern consist of high spike frequency during failing and low one during rising temperatures.

• Korean Journal of Environmental Biology
• /
• v.30 no.2
• /
• pp.143-150
• /
• 2012

To determine the spatial distribution of marine invertebrate communities in intertidal rocky shore on Dokdo, 10 times investigation was carried out at 7 stations during the year of 2009~2011. The marine invertebrate community can be divided into three different groups based on cluster analysis of Bray-Curtis similarity. As a first group, the pebble beach community, Omphalius rusticus, Monodonta perplexa, and Chlorostoma lischkei which were movable gastropod with dominant occurrence. Secondly, the still rocky shore community, O. rusticus, Serpulorbis imbricatus and Aplysia kurodai occurred dominantly. Finally, the wave-exposed rocky shore community which dominantly occurred by two sessile arthropods, Barnacles, Chthamalus challengeri and Pollicipes mitella. The three groups were likely to be determined by texture of substrate and hydrodynamic conditions of rocky shore in Dokdo. The results indicated that diversity of marine intertidal invertebrate community around Dok do was mainly affected by habitat characteristics and the surrounding physical properties.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2016.05a
• /
• pp.77-77
• /
• 2016

최근 기후변화로 인한 수문기상학적인 극한 사상들은 점점 대형화되고 있고, 그 발생빈도 또한 잦아지고 있다. 인구의 증가와 급격한 도시화, 자산 가치의 증가 등으로 물과 관련된 재해로 인한 피해는 점점 더 규모가 커지고 있다. 홍수와 가뭄, 허리케인, 쓰나미와 같이 물과 관련된 재해는 그 영향을 받는 사람들의 수로 본다면 지구상의 재해 중 90%를 차지하고 있을 만큼 그 규모가 크다 할 수 있으며, 전세계적으로 물관련 재해로 인한 재산상의 피해를 약 1,000억 달러 규모로 추산하고 있는데, 2030년에는 그 현재의 두 배가 될 것으로 예측하고 있다. 이와 같은 재해로 인한 피해는 개도국이나 최빈국뿐만 아니라 관련 인프라가 잘 구축되어 있는 선진국 또한 예외는 아니다. 2015년 9월 UN 세계지속가능 정상 회의에서 각국의 수반들 또한 17개의 "지속가능개발목표(Sustainable Development Goals; SDGs)"를 채택함으로서 post-2015 아젠다가 세계를 지속가능하고 균형 있게 바꾸어 나가기 위해서 취해야 하는 가장 시급하고 필요한 과감한 혁신적인 조치임을 인식하였다. 재해경감과 지속가능개발은 2005년 채택된 "효고프레임웍(Hyogo Framework for Action) 2005-2015"에서 도 중요하게 다루어 졌다. 2015년 3월 제3차 세계재해경감대회에서 채택된 "센다이 프레임웍(Sendai Framework for Disaster Risk Reduction) 2015-2030"은 Post-2015 개발의제의 첫 번째 합의 결의안이라 할 수 있으며, 인명피해의 실질적인 감소와 재해에 의한 영향으로 피해 보는 사람들의 수를 줄이고, 경제적 손실과 대형 인프라 피해의 경감을 주요 타겟으로 하고 있다. 이와 같은 리질리언스의 중요성은 SDGs의 Goal 11인 "안전하고 지속가능한 도시와 정주지 조성(Make cities and human settlements inclusive, safe, resilient and sustainable)"에서 강조되고 있을 뿐만 아니라 다양한 골에서 재해로 부터의 리질리언스 확보에 대한 필요성을 강조하고 있다. 재해 위험을 경감시키기 위해서 국제적으로, 지역적으로 또는 국경을 넘어서는 협력 관계의 구축이 중앙정부나 지방정부를 비롯한 국가적으로 절대적으로 필요한 노력이라 할 수 있다. 특히나, Post-2015 개발 아젠다에 대한 기후변화와 재해경감을 위한 금융지원을 포함한 최빈국, 개도국, 군소 도서국가들과 중견국 선진국들의 양자간이나 다자간 협력 채널을 통한 역량 강화가 필요하다.

• Analytical Science and Technology
• /
• v.29 no.1
• /
• pp.10-18
• /
• 2016

Phosphodiesterase (PDE) plays an important role in cAMP-mediated signaling within cells. We previously showed that the long-form of Aplysia PDE4 (ApPDE4) was localized in the plasma membrane and the presynaptic terminal in Aplysia sensory neurons, and the 16 N-terminal amino acid was sufficient for this targeting process. In this study, we characterized the cellular localization of various ApPDE4 mutants. We first identified the roles of each amino acid within the group of 16 N-terminal amino acids of long-form ApPDE4. As a result, we were able to identify various mutants that were localized to both the plasma membrane and the Golgi complex, Golgi only, or both the endoplasmic reticulum (ER) and the Golgi complex. To examine the role of palmitoylation on the cellular localization of ApPDE4 mutants, 2-bromo palmitate (2-BR) was used as a treatment. As a result, in the presence of 2-BR, the plasma membrane targeting of many mutants was impaired, indicating that palmitoylation was involved in the plasma membrane targeting of the mutants. We also found that PI4P play crucial roles in the Golgi targeting of (N16,C3S/VV/G)-mRFP, L(N16,C3S/LFS/R)-mRFP, and L(N16,EPL/R)-mRFP.

• Analytical Science and Technology
• /
• v.26 no.1
• /
• pp.106-112
• /
• 2013

PDE plays an important role in cAMP-mediated cellular signaling within the cells. The proper targeting of each PDE is mediated by unique N-terminal of each PDE isoform. It has been recently reported that supershort-, short- and long-forms of PDE4 in Aplysia were cloned in Aplysia. Long-form of ApPDE4 was localized at plasma membrane and presynaptic terminal in Aplysia sensory neurons. However, it remains elusive which part of ApPDE4 is minimal region for the proper targeting and what are the effects on the cell functions. Here, we identified that N-terminal 13 amino acids of ApPDE4 long-form is minimal regions for the plasma membrane targeting. In addition, overexpression of ApPDE4(N20)-mRFP could induce morphological changes in HEK293T cells. Interestingly, mRFP-$PLC{\delta}1$(PH), which selectively binds to PI4,$5P_2$, could induce morphological changes in similar with that by ApPDE4(N20)-mRFP. These results suggested that binding of ApPDE4(N20) to lipids including PI4,$5P_2$ might be responsible for targeting of ApPDE4 to plasma membrane and morphological changes in HEK293T cells.

• Journal of Life Science
• /
• v.28 no.2
• /
• pp.162-169
• /
• 2018

Many cytoplasmic proteins are targeted to the cytoplasmic membrane of the trans-Golgi network (TGN) via an N-terminal short helix. We previously showed that the 20 N-terminal amino acids of Aplysia phosphodiesterase 4 (ApPDE4) long form are sufficient for its targeting to the plasma membrane and the TGN. The N-terminus of the ApPDE4 long form binds to PI4P and sulfatide in vitro. Therefore, in order to decipher the roles of sulfatide in Golgi complex targeting, we examined the cellular localization of sulfatide-binding peptides. In this study, we found that enhanced green fluorescent protein (EGFP) fused to the C-terminus of modified sulfatide- and heparin-binding peptides (mHSBP-EGFP) was localized to the TGN. On the other hand, its mutant, in which tryptophan was replaced with an alanine, leading to the impairment of heparin and sulfatide binding, was localized to cytosol. We also found that the TGN targeting of mHSBP-EGFP is impaired by the treatment of antimycin A, phenylarsine oxide (PAO), and adenosine but not a high concentration of wortmannin. These results suggest that PAO and adenosine-sensitive kinases, including phosphatidylinositol 4-kinase II, may play key roles in the recruitment of mHSBP-EGFP.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.30 no.5
• /
• pp.796-801
• /
• 2001

A total of 277 mineral spring water samples in Kangwon province from 1999 to 2000 were analyzed for the presence of Yersinia spp. by the conventional Food and Drug Administration protocol, and presumptive strains were identified by morphological, cultural and biochemical tests according to Bergey’s manual. Also, the biotypes, serotypes, and susceptibility to 12 antibiotics were tested. Among the total 277 mineral spring water samples, 40 samples (14.4%) were found to be contaminated with Yersinia species. Among the 40 strains of Yersinia spp. isolates, 33 strains (82.5%) for Yersinia enterocolitica, 4 strains (10%) for Yersinia frederiksenii, 2 strains (5%) for Yersinia intermedia, and 1 strain (2.5%) for Yersinia sakazaki were identified, respectively. Of 40 Yersinia spp. isolates, Yersinia enterocolitica (82.5%) was the most predominant species in the mineral spring water samples compared to other Yersinia species. Compared to direct culture method after KOH treatment and KOH treatment method after cold enrichment for better isolation ratio of according to comparision of Yersinia species, the detection ration (18.5%) of KOH treatment method after cold enrichment was about 3 times better than that (6.1%) of direct culture method after KOH treatment. According to serotypes of Y. enterocolitica isolates, O : 5 (12.9%) was the most predominant and followed by O : 3 (9.7%), O : 8 (6.5%), and O : 9 (3.2%), and others. For biotypes of Y. enterocolitica isolates, 1A (71.0%) was the most predominantly abundant and followed by 3A (12.9%), 3B (9.7%), 1B (3.2%) and 5 (3.2%). Also, an antibiotic susceptibility test showed that Yersinia spp. isolates were very susceptible to the antibiotics tested, but they were very strongly resistant to ampicillin, cephalothin and carbenicillin.