• Korean Journal of Environment and Ecology
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• v.28 no.3
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• pp.273-280
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• 2014

This study examined whether Dybowski's brown frogs(Rana dybowskii) in noisy highway roadsides had different mating calls from those in natural sites. We selected four study sites: two rice paddy sites in Youngdong Highway roadside and two nearby natural counterparts. Frog calls were recorded between 18:30 and 20:00 on February 24, February 27, and March 14, 2014. Frog calls in the natural sites had fundamental frequency approximately at 700 Hz with two to four apparent harmonics, while frog calls in the highway sites had higher fundamental frequency with up to seven apparent harmonics. Analysis of variance (ANOVA) shows that a roadside site that are directly exposed to highway noise had statistically higher frog calling frequency than other study sites. However, the higher calling frequency was not found in another roadside site that differed in elevation from the highway and was buffered by forests. These results indicate that male frogs in a noisy highway roadside called females with a higher pitch and more apparent harmonics to avoid being masked by highway noise. These results also suggest that there is a threshold noise level that interrupts frog's mating behavior and it is needed to maintain highway roadside noise to this threshold level.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.369-374
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• 2003

Nonylphenol (NP), an well known aquatic contaminant, has been known to induce abnormalities in various aquatic animals. In an effort to develop proteome in the study of aquatic contamination of NP and its impact on the amphibia, protein changes in liver tissues of Korean red bellied frog, Bombina orientalis was investigated following the NP exposure. NP was administered intraperitoneally to male B. orientalis at 10 mg/kg body weight. At 48 and 96h after the treatment, the frog livers were sampled, and the protein fraction was separated using two dimensional gel electrophoresis (2D/E) and visualized with Coomassie brilluant blue staining. The 2D/E Images of the tissue from the animals treated with NP showed marked changes of protein spots (about 20% of total protein spots). Analysis of the 50-60 separated spots allowed identification of the major protein changes in the overall pattern for the stressor (NP) by time (0,48 and 96 h). At 48h after treatment, 8 spots were increased and 12 spots were reduced. Then, at 96h after treatment, 10 spots were increased and 8 spots were reduced. In total, approximately 29% of liver proteins showed the altered expression following the NP treatment. It is suggested that protein expression was repressed by blocking of certain metabolisms at 48 hand induced by the synthesis of new proteins for adaptation at 96 h following NP exposure. This application for 2D/E analysis may show promise in searching biomarkers for environmental proteomics in amphibians.

• Korean Journal of Ecology and Environment
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• v.53 no.3
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• pp.221-228
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• 2020

We studied the development of the endangered gold-spotted pond frog, Pelophylax chosenicus eggs and analyzed growth and survival rate of the tadpoles while feeding different feed to obtain the basic data on the its biological traits and reproduction. We observed the fertilized eggs of gold-spotted pond frog that naturally spawned in a breeding tank using a microscope. Fertilized eggs were sphere shape surrounded by colloid layers, clearly distinct with animal and plant poles. The fertilized eggs hatched 31 hours after fertilization and operculum complete at 175 hours after fertilization at water temperature of 27.0±1.0℃. As a result of growth and survival rate by different feed from 35 days after mouth open stage, vegetable feed (VF) group and tetra-min (T) group were significantly higher average weight gain rate (WG)(5843.9% and 5736.3%, respectively) and average specific growth rate (SGR) (11.67% and 11.62, respectively) compared with other groups (WG: 641.8~4625.2%, SGR: 5.72~11.01%). The vegetable (V) group showed the lowest growth rate (P<0.05). The average feed efficiency was 177.83% in the VF group, about 17 times higher than the V group. In addition, the average survival rate of the VF and T groups were the high at 97.5% and 100%, respectively, and the V group was the lowest at 32.5%. Therefore, the more efficient feeds for successful breeding is singly with VF or T.

• Korean Journal of Soil Science and Fertilizer
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• v.15 no.2
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• pp.141-146
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• 1982

Laboratory and greenhouse experments were conducted to find out the optimum dilution time of methane fermented effluent of hog waste which was high pH (7.85), $NH_4$-N (683ppm), electric conductivity (8.3mmhos/cm) and BOD (about 1,000ppm) and the interval of effluent change which will provide maximun yield of green algae (Scenedesmus accuminatus), duck weed (Lemner minor L.) and Azolla anabaena (Azolla pinnata). The results of this experiment are summerized as follows. 1. The maximum yield were obtained at 5 times diluted effluent ($NH_4$-N : 137ppm) and exchange of 4 day intertval, from 25 times to 50 times dilution ($NH_4$-N : 7-14ppm) and 4 day interval, and 50 times dilution ($NH_4$-N : 14 ppm) and exchange the effluent at 4-8 day interval for green algae, duck weed and azolla growth, respectively. 2. Purification of the effluent was enhenced by growth of the green algae, duck weed and azolla due to decrease of pH, $MH_4$-N and electric conductivity of effluent. The degree of purification was higher in case of azolla growth than duck week growth.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.706-714
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• 2017

An investigation of the effects of Pb for domestic anuran embryos were evaluated with the Frog Embryo Teratogenesis Assay; Xenopus (FETAX). Depending on the species, the difference between the embryo size and the embryonic development time was determined. As a result, mortality and malformation rates were increased, malformation patterns were changed and larval body length were decreased in a dose dependent manner of the Pb. The half maximal lethal concentration ($LC_{50}$) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.58, 0.49, 0.52, $0.54mg\;L^{-1}$, respectively. The half maximal effective concentration ($EC_{50}$) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.35, 0.74, 0.30, $0.29mg\;L^{-1}$, respectively. The teratogenic index (TI) were 1.66 in the Bufo gargarizans, 1.81 in the Hyla japonica, 1.73 in the Rana nigromaculata and 1.86 in the Bombina orientalis, respectively. Therefore, the Pb seems likely to have a teratogenic effect in all four species' embryonic development. The Bombina orientalis was the most sensitive to the Pb. This means that the difference between the different species, even if they have all been exposed to the same concentration of pollutants depending on the species. The result above show that the Pb acts as a teratogenic agent in the development of the four domestic frog species.

• Korean Journal of Environmental Biology
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• v.39 no.3
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• pp.311-318
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• 2021

In this experiment, we investigated the toxicity of tebuconazole (fungicide) using domestic frog embryos, along the FETAX (Frog Embryo Teratogenesis Assay-Xenopus) protocol. Bufo gargarizans, Hyla japonica, and Pelophylax nigromaculatus embryos were incubated, and investigation of the tebuconazole effect was performed by the probit analysis. As a result, depending on the concentrations of tebuconazole, the mortality and malformation rates were increased and larval body length was decreased. The teratogenic concentrations (EC₅₀) of tebuconazole were 34.4mg L^-1, 10.6mg L^-1, and 14.9mg L^-1, respectively, and the embryo lethal concentrations(LC₅₀) of tebuconazole were 74.7 mg L^-1, 38.5 mg L^-1, and 39.1 mg L^-1, respectively. The teratogenic index (TI) valuesof tebuconazole were 2.19, 3.58, and 2.65; thus, it showed teratogenicity in embryonic development of these three frogs. These results revealed that in this experiment, tebuconazole suppressed the development of embryos at a relatively low concentration. In addition, mortality, malformation ratios, malformation patterns, and growth rates were similar to the results from the other assay systems. Therefore, tebuconazole was thought to have an effect on the embryo development of domestic frogs. In future, it will be necessary to identify species specificity in order to the clarify the causes of differences in mortality, malformation rate, and malformation patterns depending on the species.

• 프린팅코리아
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• s.18
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• pp.86-93
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• 2003

우리 나라가 출판 대국이라고는 하지만 어디까지나 내수 측면의 일이다. 자괴나 자기 비하를 할 필요까지는 없지만, 해외 도서시장 진출에 관한 한 우리 출판계는 아직까지 우물 안 개구리 신세에 가깝다. 국내에서 치열한 경쟁을 벌여야 하고 자본 규모도 크지 못한 대부분의 출판사 입장에서 저작권 수출은 남의 이야기로만 들릴 가능성도 크다. 더구나 전례로 삼을만한 사례들이 참고 자료로서의 의미를 지닐 정도로 충분히 축적되어 있지도 못하다.

• 건강소식
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• v.36 no.3
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• pp.20-22
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• 2012

겨울잠을 자던 개구리가 깨어나는 경칩이 지나고 밤과 낮의 길이가 같아지는 춘분이 오면 기다리고 기다리던 봄이다. 하지만 오는 봄이 고통스러운 사람이 있다. 꽃가루, 황사 등으로 알레르기성 비염을 앓고 있는 사람과 알레르기성 결막염을 앓고 있는 사람이 그 주인공이다.

• 정보화사회
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• s.180
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• pp.40-41
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• 2006

한 단계 도약을 위한 준비운동 차원이랄까, 일본의 이동전화 서비스 사업자 및 제조 업체들이 연이어 인수합병을 추진하고 있다. 내수를 발판으로 국제무대로 진출하고자 하는 의지와 표명으로 풀이된다. 2G의 자국 표준으로 PDC를 선택해 '우물안 개구리' 신세를 면치 못한 한을 3G 시대에서 말끔히 풀겠다는 결의까지 느껴지기도 한다. 최근 변화를 모색하고 있는 일본 이동전화 시장 동향을 짚어봤다.

• Journal of the Speleological Society of Korea
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• v.5 no.6
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• pp.20-22
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• 1980

동굴 속이라는 특수환경 속에 사는 동물을 우리는 동물생물(Gavernicole)이라 부르며, 이 동굴생물은 i)외래성(trogloxene) : 개구리(Amphibia) 따위의 양서류와 파리(Piptera) 등. ii) 호동굴성(troglophile) : 푸라나니아(Tricladia), 거미류(Araneido) 박쥐(Chirptera) 등. iii) 진동굴성(troglobiont): 새우류(Amphipoda), 톡톡이(Collembala), 좀류(Thysanura), 노래기(Aiplopada) 등. 3가지로 분류하고 있다.(중략)