• Title/Summary/Keyword: 감수분열상

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Embryological Characteristics on Seed Sterility of Ligusticum chuanxiong Hoit. (천궁의 종자불임에 관한 발생학적 특성)

  • Park, Chung-Heon;Lee, Man-Sang;Namkoong, Seung-Bak;Yu, Hong-Seob;Park, Hee-Woon
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.3
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    • pp.209-213
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    • 2004
  • Ligusticum chuanxiong is receiving much attentions as one of the important medicinal crops with the increasement of the crude drug demands. This study was conducted to obtain the basic informations of breeding of Ligusticum chuanxiong. Embryological characteristics were examined to elucidate the process of male and female gametophytic development and fertilization. Meiosis and nucleus division of megaspore and microspore were proceeded normally. With regard to the formation of female gametophyte, only a half of female gametophyte developed to normal egg apparatus. While another 50% showed abnormal egg apparatus with poly-nuclei or non-nuclei ovule. The pollens developed from the micros pore were formed more than 90 % of normal pollen. It was difficult to observe fertilization because ovule tissue was very compact and cell was extremely tiny, but could be easely observed proembryo and embryo formation. Only 30 percent developed into proembryo and subsequently into embryo, and the others were degenerated.

Influence of Mutagen at Meiotic Stage on Wheat Ploidy in Anther Culture (밀 감수분열기 Mutagen 처리가 약배양에 있어서 염색체 변이에 미치는 영향)

  • 박광근;강양순;하용웅;허한순
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.1
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    • pp.74-78
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    • 1991
  • This experiment was conducted to know the effects of mutagen treatments on callus induction, plant regeneration and their ploidy in the anther culture of wheat. The winter wheat cultivars, 'apos;and 'apos;Wonkwang'apos;, were treated at the mid or late-uninucreate stage under 4 different doses (100, 200, 500 and 1,000 rad.) of X-ray and 3 different levels(0.1, 0.2 and 0.3 mole) of Ethyl Methane Sulphonate. The anthers treated were set on the C$\_$17/medium for callus induction, and callus induced was transfered to 1/2 MS medium for plant regeneration. The mutagen treatments inhibited the callus induction but increased the plant regeneration in the callus which were induced from the anther set on the medium for the long time of 60 to 80 days. Also, the chromosome number to the regenerated plant varied largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.aried largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.

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Dose-Response Relationship of Micronucleus Frequency in Pollen Mother Cells of Tradescantia (자주달개비 화분모세포 미세핵 생성률의 방사선량-반응 관계)

  • Kim, Jin-Kyu;Song, Hi-Sup;Hyun, Soung-Hee
    • Journal of Radiation Protection and Research
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    • v.24 no.4
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    • pp.225-230
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    • 1999
  • This study was carried out to investigate the radiation dose-response of micronucleus frequencies in Tradescantia pollen mother cells. The number of micronuclei increased in the tetrads as a result of chromosome deletion after irradiation. The maximal frequency of micronuclei showed a good dose-response relationship in the range of dose $0{\sim}50$ cGy. On the basis of the relationship, a dose of 1 cGy results maximally in two additional micronuclei in 100 tetrads. The radiation dose-response relationship of micronucleus occurrence is prerequisite to biological monitoring of radiations. The micronucleus assay can be applied to biological risk assessment of environmental toxicants, and to integrity test of water or soils of interest.

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Gametophyte formation and Microspore Germination in Astragalus membranaceus Bunge (황기의 배우체 형성과 소포자 발아 특성)

  • 김영국;손석용;성낙술;이봉호;박종선
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.6
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    • pp.443-448
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    • 2001
  • This study was carried out to obtain the basic informations on the characteristics of gametophytes formation and microspore germination in Astragalus membranaceus Bunge. Pollen mother cells passed through meiosis when the flower bud length reaches around 3.5 mm, thus creating the tetrad when it is 4.0 mm long. Pollen attains full growth when the bud is about 10.0 mm long and the anther is found to dehisce when the length of tate bud reach around 12.0 mm. Embryo sac develops at a similar speed as pollen did and it attains its full growth when the bud is about 10-12 mm long. After being stored at 4$^{\circ}C$ or -4$^{\circ}C$, the pollen maintained its germination ability to almost full extent by the 30th day after store. However, the germination rate at room temperature (23~28$^{\circ}C$) decreased below 3% by the 3rd day of storage and so did the germination speed.

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Effect of Nitrogen level under low Temperature Condition on Growth Characters, Nitrogen Concentration and Ethylene Evolution of Rice Varieties (저온하에서 질소시비량이 수도품종의 생육형질, 질소함량 및 ethylene 생성량에 미치는 영향)

  • Lee, Jong-Hoon;Lee, Moon-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.2
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    • pp.215-223
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    • 1987
  • This experiment was carried out to determine the effect of nitrogen application on the cold tolerance of rice plant, with treatment of three levels of nitrogen and three times of application under the low temperature at tillering and panicle initiation stages. The higher cold tolerance variety was increased in plant height and number of tillers on high nitrogen level during the low temperature treatment. Nitrogen content of leaf blade was increased, but carbohydrate content was decreased during the low temperature treatment at tillering stage. Ethylene evolution from leaf was remark-ably increased just after low temperature treatment. Highly significant negative correlation was observed bet-ween the nitrogen content of leaf blade and percentage of filled grain under low temperature condition at reproductive growth stage.

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Physiological Studies on Injuries of Cool Weather in Rice Plant I. Effect of Heading Date and Physiological Characteristics of Boron and Phosphorus Application Under the Cold Water Temperature in Rice Plant (수도의 냉해에 대한 생리학적 연구 제1보 저수온하에서 붕소 및 인산시용이 수도의 출수 및 생리적 특성에 미치는 영향)

  • Cho, D.S.;Heu, H.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.1
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    • pp.76-80
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    • 1983
  • In order to investigate the effect of boron and phosphorus for reduction of cold injury of rice, this experiment was undertaken by pot trial. Two levels of phosphorus and 6 levels of boron were applied once at the three stages such as tillering, panicle formation and meiosis stage. Cold water was irrigated to maintain cool temperature ranging 15 to $18^{\circ}C$ from panicle formation stage. It was shown a tendency that three to seven days of early heading were resulted by the 3, 5 and 10kg of boron application per 10 are. The ripening ratio and physiological root activity was rather slightly increased in the above boron levels.

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Studies on the Effect of Low Temperature Treatment at Meiotic, Heading and Seedling Stage in Paddy Rice (수도의 장해형 냉해에 관한 연구)

  • Hong-Suk Lee;Hyung-Yull Cho;Pyeong-Ki Yim;Hoon Heu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.15
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    • pp.85-97
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    • 1974
  • In order to clarify the inducing conditions and cause of sterility in rice plants, 4 varieties were cooled at 3 different levels of temperature combined with 3 different levels of treatment period. And 19 varieties were tested to examine the varietal difference of cold resistance. The results obtained were summarized as follows; 1. There were significant varietal differences in the effect of cooling treatment at meiotic stage. Suwon 213-1 was induced heavy sterility by 3 day cooling treatment at 17.5$^{\circ}C$ whereas Hayayuki, Nongpaik and Jinheung were induced a little sterility by 3 day cooling treatment at 15$^{\circ}C$ and 5 day treatment at 17.5$^{\circ}C$. The per cent of grain fertility was correlated significantly with the delayed days to heading, the degree of panicle extraction (Suwon 213-1, Nongpaik, Jinheung), culm length (Nongpaik, Suwon 213-1), and Auricle distance (Suwon 213-1). The degree of sterility was able to be estimated from the linear regression equation between the degree of panicle extraction (distance from panicle neck to flag leaf) and fertility percentage. In the case of heavy cold damage by the treatment of low temperature at meiotic stage, the rice plant had somewhat lower pollen density per anther, small and ununiform anther and pollen in size, and more sterile pollen grains. Suwon 213-1 showed anthesis in almost all spikelets, while Nongpaik, Jinheung and Hayayuki indicated considerable number of indehisced anther at 5 days after heading. 2. The fertility were not generally higher in cooling treatment at heading stage than at meiotic stage treatment. And significant correlation was found between the percentage of grain fertility treated at above two stages. Nongpaik and Jinheung were not affected in percentage of fertility by 5 day treatment at 15$^{\circ}C$ when these were treated at heading stage. Indehisced anthers were not found in Suwon 213-1 and Hayayuki, but Nongpaik and Jinheung showed more anthers which did not show anthesis 3. There was different varietal response to low temperature which was indicated by the decrease of grain fertility resulted from cooling treatment at meiotic stage. Jaekeun and Jinheung did not show low fertility but Milseong, Suwon 210, Satominoli and Suwon 213-1 showed outstanding decrease in fertility percentage by the cooling treatment at meiotic stage. The varieties which had low fertility were likely to have low pollen density per anther, abnormal anthers, small size po]]en grains and many sterile pollens. 4. Remarkable varietal difference of cold resistance was found in heading stage cooling treatment. Nongpaik, Jinheung, Jaekeun, Paltal, Akibare, Milseung and Palkeong were not affected in grain fertility by cooling treatments but Nonglimna No. 1, Suseong, Hayayuki, Suwon 213-1 and Suwon 210 showed significantly high sterility as treated by cool temperature. Most of the varieties showed higher fertility by cooling treatment at heading stage than meiotic stage but Hayayuki, Suseong and Nonglimna No.1 showed lower fertility when these were treated at heading stage than meiotic stage. There were two grops of varieties in the response to cooling treatment, one was somewhat non-anthesised and the other showed full anthesis. 5. In cold injury test of young seedlings, the result of observation was not accorded with the degree of growth inhibition. As a general, Palkeum and Suseong were highly torelant to cool temperature but Suwon 213-1, Jaekeun, Paltal, Shirogane, Palkeong, Mankyung were highly susceptible. 6. There is no significant correlation between the degree of young seedling cold damage and or the degree of growth retardation at seedling stage and grain fertility resulted from coding treatment both heading and meiotic stage.

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Carbohydrate Requirements of Follicular Bovine Oocytes Cultured in a Chemically Defined, Hormone-, Amino Acid- and Protein-Free Medium (호르몬, 아미노산 및 단백질이 첨가되지 않는 단순배양배지내 소 난포란의 탄수화물 요구량)

  • 임정묵
    • Development and Reproduction
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    • v.1 no.1
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    • pp.1-8
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    • 1997
  • 본 연구는 단순배양체계내 소 난포란의 성숙에 요구된는 탄수화물 (glucose, lactate와 pyruvate)을 검토하기 위해 수행하였다. GV(germinal vesicle) 단계의 난구세포로 둘러싸인 소 난자를 단백질, 아미노산 및 호르몬이 첨가되지 않은 modified Tyrodes (mT)에서 24시간, 5% $CO_{2}$ 배양기를 이용하여 성숙배양하였다. Glucose 무첨가군 (0-61%)에 비해 5.6mM의 glucose 첨가군 (71-74%)이 유의적으로 높은 (P<0.05) M-II단계로의 난자 발육을 나타내었다. Glucose를 함유한 배지내에서는 lactate(10mM0와 pyruvate(0.5mM)의 첨가에 따르는 M-II 단계로의 발육에 있어 차이를 보이지 않았다. 그러나 glucose 무첨가 배지에서는 pyruvate와 lactate를 첨가하는 것이 첨가하지 않은 것에 비해 condensed GV(76%vs. 0-2%), M-II, (43-61%vs. 0%)단계에 이른 난자수가 유의적으로 높았다. Glucose 함유 mT배지에 lactate와 pyruvate를 첨가하여 난자를 배양하였을 때, 동결융해 정자와 24시간 정치한 후 난자중 87-93%가 정자침투되었고 39-44%가 전핵단계로 발육하였다. 침투난자의 26-30%는 다정자수정이었다. 결론적으로, GV 단계의 소 난포란은 에너지원으로 lactate, glucose와 Pyruvate를 이용하지만, 감수분열 성숙을 유지하는데 glucose가 가장 효과적이다. 이 결과들로 보아 단순배양배지는 소 난포란의 체외성숙에 영향을 미치는 다양한 물질들을 연구하는데 잠재적으로 유용하다.

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Current status and prospects of the meiosis-specific function of recombinase in plants (식물의 감수분열에서 상동 재조합 효소 특이 기능의 연구현황 및 전망)

  • Jung, Yu Jin;Nam, Ki Hong;Kim, Tae Sung;Lee, In Hae;Cho, Yong-Gu;Kang, Kwon Kyoo
    • Journal of Plant Biotechnology
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    • v.45 no.1
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    • pp.1-8
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    • 2018
  • Meiosis is a specialized cell division, essential in most reproducing organisms to halve the number of chromosomes, thereby enabling the restoration of ploidy levels during fertilization. A key step in meiosis is homologous recombination, which promotes homologous pairing and generates crossovers (COs) to connect homologous chromosomes until their separation at anaphase I. These CO sites, seen cytologically as chiasmata, represent a reciprocal exchange of genetic information between two homologous non-sister chromatids. RAD51, the eukaryotic homolog of the bacterial RecA recombinase, plays a central role in homologous recombination (HR) in yeast and animals. Loss of RAD51 function causes lethality in the flowering plant, Arabidopsis thaliana, suggesting that RAD51 has a meiotic stage-specific function that is different from homologous pairing activity.

Characterization of Spermatogonial Stem Cells and Testicular Cells in Chicken

  • Lee, Bo Ram;Lee, Young Mok;Park, Tae Sub;Jung, Jin Geyoung;Hong, Yeong Ho;Lim, Jeong Mook
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2003.11a
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    • pp.69-70
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    • 2003
  • According to topographical methods, the chicken spermatogonia was located in basal membrane of seminiferous tubules. It has large nuclei and mitochondria and proliferated with cellular bridges. Immunohistochemistry data showed that anti-SSEA-1 antibody specifically reacted with $\textrm{A}_{pr}$ and $\textrm{A}_{al}$ type spermatogonia. Lectin, STA and integrin-6, -1 were also specific to $\textrm{A}_{s}$ type spermatogonia.

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