• Journal of Radiation Industry
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• 제7권2_3호
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• pp.161-166
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• 2013

The purpose of this study was to investigate the cell proliferating and interleukin-2 producing activity of staphylococcal enterotoxin B by gamma-irradiation. Staphylococcal enterotoxin B was gamma-irradiated with the various doses of 0, 2, 20 and 50 kGy. SDS-PAGE analysis showed that gamma-irradiation caused the sharp decrease of the content of staphylococcal enterotoxin B and the effect was irradiating dose-dependent. Non-irradiated staphylococcal enterotoxin B increased the cell proliferation of splenocytes isolated from female Balb/c mouse, whereas 2 kGy-irradiated toxin significantly decreased the activity. 20 and 50 kGy-irradiated staphylococcal enterotoxin B was no effect. A similar effect on the interleukin-2 production of mouse splenocytes was observed with non-irradiated and irradiated staphylococcal enterotoxin B. It was considered to be due to the decrease of the antigenicity of staphylococcal enterotoxin B by gamma-irradiation. Therefore, these results suggest that gamma-irradiation can be effective for the decrease of the antigenicity of staphylococcal enterotoxin B as superantigen.

• Journal of Radiation Industry
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• 제7권2_3호
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• pp.93-99
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• 2013

This study was conducted to evaluate the effect of gamma irradiation on the microbiological and sensory characteristics of frozen Bibimbap. Frozen Bibimbap vegetables were contaminated by microbial levels of $2.70{\sim}6.67log\;CFU\;g^{-1}$ in total aerobic bacteria. Frozen Bibimbap was contaminated by microbial levels of $4.8log\;CFU\;g^{-1}$ in total aerobic bacteria, but microorganisms in gamma-irradiated samples at above 25 kGy were not found at detection limit less then $1log\;CFUg^{-1}$. Sensory characteristics of the gamma-irradiated Bibimbap were decreased depending upon the irradiation dose, and sensory scores of the samples irradiated with doses higher than 10 kGy were significantly decreased. Therefore, the application of combination of the various food processing technology should be considered for the sterilization of Bibimbap, without any deterioration of quality occurred by high dose irradiation.

• Journal of Radiation Industry
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• 제5권2호
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• pp.175-178
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• 2011

This study was conducted with effect of lactic acid bacteria by gamma irradiation. Lactic acid bacteria were exposed to irradiation with a single absorbed dose of 1, 2, 3, 4, 5, 6, 8, and 10 kGy. Possible lactic acid bacteria, including Lactobacillus paracasei KCTC 13169, Lactobacillus casei KCTC 3109, Lactobacillus acidophilus KCTC 3140, Lactobacillus plantarum subsp plantarum KCTC 3103, Lactobacillus debruekii subsp bulgaricus KCTC 3635, Streptococcus thermophilus KCTC 3658 were selected. The radiation sesitivities of lactic acid bacteria were expressed as $D_{10}$ values. The $D_{10}$ values of Lactobacillus paracasei, Lactobacillus casei, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus debruekii were calculated as 0.42, 0.51, 0.47, 0.90, 0.44, and 0.61 kGy, respectively. Results suggest that L. acidiphilus has the highly resistant to gamma irradiation.

• Journal of Radiation Industry
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• 제4권1호
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• pp.53-57
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• 2010

Cooking drips which were obtained as by-product after seafood processing in the food industries, still contain lots of proteins, carbohydrates, and other functional materials. But, the seafood cooking drips are easily contaminated because of its rich nutrients, and their color are very dark. This study was conducted to investigate the effect of gamma irradiation on the quality of seafood cooking drips including Hizikia fusiformis, Enteroctopus dofleini, and Thunnus thynnus. The Hunter's color values (L, Brightness) of H. fusiformis, and T. thynnus were increased with increasing irradiation doses, showing becoming bright. The crude protein content and crude lipid content were increased by gamma irradiation. These results indicated that gamma irradiation increased extraction efficiency of available compounds in cooking drips.

• Journal of Radiation Industry
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• 제5권2호
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• pp.125-130
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• 2011

The purpose of this study was to evaluate the protective effect of the Perilla frutescens cv. Chookyoupjaso mutant water extract (PFWE) on gamma ray-induced oxidative stress in mice. Gamma-ray is one of the sources for inducing oxidative stress. The study was divided into 6 groups with 6 mice for each treatment. Groups I and II were treated with saline (vehicle) only, groups III, IV, V, and VI were pretreated with PFWE 10, 20, 50, $50mg\;kg^{-1}$ respectively for 2 weeks before gamma radiation. And then groups II, III, IV, V were irradiated. We found that the activities of aspartate transaminase (AST) and alanine transaminase (ALT) were increased and the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were decreased by irradiation in mice. However, treatment of PFWE attenuated the activities of AST and ALT in a dose-dependent manner in irradiated mice. Furthermore, treatment of PFWE significantly increased the activities of SOD, GPx, and GR in a dose-dependent manner in irradiated mice, except for the CAT. Interestingly, the activities of GPx and GR were significantly increased by PFWE treatment. Taken together, PFWE could be effective in protecting on gamma ray-induced oxidative stress in mice.

• Journal of Radiation Industry
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• 제6권2호
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• pp.147-152
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• 2012

Anthocyanins are major plant pigment and produced through phenylpropanoid pathway. In this study, anthocyanin biosynthesis mechanisms of chrysanthemum flowers were studied using 'Argus' and flower color mutant 'ARTI-purple' which were induced by 40 Gy gamma irradiation ($Co^{60}$). And, three chrysanthemums, 'Ford', 'Yeonja' and 'Orando' were additionally used as the check varieties to understand the relationship between flower color and expression patterns of genes. The expression patterns of the anthocyanin biosynthetic genes were matched with the flower color of the check varieties. High anthocyanin concentration of 'Orando' showed the high expression of anthocyanin biosynthetic genes. In the white flower of 'Ford', expressions of CHI, DFR and ANS were not identified. Despite different flower color, 'Argus' and 'ARTI-purple' showed different expression patterns compared with the check varieties. From the dot blot analysis, we screened the seven genes showing the different expressions between 'Argus' and 'ARTI-purple'.

• Journal of Radiation Industry
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• 제4권4호
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• pp.313-317
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• 2010

The struvite crystallization was applied to remove $NH_4{^+}$ in livestock wastewater. The ammonium ions can be very toxic to the aquatic creatures. In this experiments, the livestock wastewater from Gongju livestock wastewater treatment plant was used. The behaviors of various parameters such as pH, mole ratio of $Mg^{2+}$ : $NH_4{^+}$ : $PO{_4}^{3-}$ and reaction temperature for struvite crystallization of livestock wastewater and the effect of gamma ray irradiation were evaluated. As results, for the pH variation, the $NH_4{^+}$ removal efficiency showed the highest, 88%, at pH 9~9.25. The removal efficiency of $NH_4{^+}$, $Mg^{2+}$ and $PO{_4}^{3-}$ was showed highest when same molar ratio of $Mg^{2+}$ and $PO{_4}^{3-}$ were applied. The $NH_4{^+}$ removal efficiency showed 82% at $7^{\circ}C$, and 90% at $30^{\circ}C$ with temperature. When the wastewater was irradiated with 20 kGy of gamma ray, $NH_4{^+}$ was removed as much as 83%.

• Korean Journal of Remote Sensing
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• 제21권2호
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• pp.99-111
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• 2005

A fuzzy logic based data integration was applied for geothermal potential mapping in Jeju Island. Several data sets, such as geological map, the density of drainage system, the distribution density of cinder cones, density of lineaments, aerial survey map for total magnetic intensity and total gamma ray, were collected as thematic map for the integration. Fuzzy membership function for all thematic maps were compared to the locations of the spa, which were used as ground-truth control points. The older geology, the lower density of drainage, cinder cones and lineaments, and the lower intensity of magnetic and gamma ray were showed the higher fuzzy membership function values, respectively. After integrating all thematic maps, the results of gamma operator with the gamma value of 0.75 was the highest success rate, and new geothermal potential zone is prospected in western north part of Jeju Island.

• Journal of Radiation Industry
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• 제12권4호
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• pp.277-285
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• 2018

Patients administered radioisotope for medical purposes are regulated by each country to quarantine them until their body's radioactivity contents decrease below release criteria. To predict the quarantine period and provide it to medical staffs and patients, it is necessary to approach the assessment of the exposure dose of persons due to patients in a realistic manner. For this purpose, a whole-body effective half-life should be applied to the dose assessment equation instead of the physical half-life. In this study, we constructed a bio-kinetic model for each nuclear species based on the ICRP publication to obtain a whole-body effective half-life of 10 unsealed gamma-ray emitting nuclei from the notification of Nuclear Safety and Security Commission, and calculated the effective half-life mathematically by simulating the distribution of the radioisotope administered in the whole body as well as each organ scale. The whole-body effective half-life of $^{198}Au$, $^{67}Ga$, $^{123}I$, $^{111}In$, $^{186}Re$, $^{99m}Tc$, and $^{201}TI$ were 1,93, 2.57, 0.295, 2.805, 1.561, 0.245, and 2.397 days respectively. However, it was found to be undesirable to offer a single value of the effective half-life of $^{125}I$, $^{131}I$, and $^{169}Yb$ because the changes in the effective half-life show no linearity. A bio-kinetic model created for the internal exposure assessment has been shown to be possible to calculate the effective half-life of radioisotopes administered in the patient's body, but subsequent studies of radiolabeled compounds are required as well.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제55권3호
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• pp.302-309
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• 2022

Low-salt fermented pollack Theragra chalcogramma roe (Myungran Jeotgal, MJ), a traditional Korean fermented seafood, was prepared using a commercially available method and irradiated using gamma rays (0-10.0 kGy) to investigate the effect of gamma irradiation (GI) on food quality. After irradiation with 2.5 kGy gamma rays, the number of viable cells in MJ was reduced by one log compared to that observed in non-irradiated MJ, and no living cells were detected after 7.5 kGy GI. Though up to 2.5 kGy GI had no effect on color quality, over 7.5 kGy GI resulted in significantly poor quality MJ surface color. Saturated fatty acid contents increased slightly in irradiated MJ, while those of polyenes and monoenes decreased slightly as the GI dose increased. Exposure of MJ to GI had no effect on the free amino acid and mineral composition. These results demonstrated that the appropriate GI dose for MJ ranged from 2.5 to 5.0 kGy, which may be recommended for industrial application.