• Applied Biological Chemistry
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• v.46 no.4
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• pp.353-360
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• 2003

For the simple rapid bioassay of organophosphorus and carbamate pesticide residues, a mass-production system of acetycholinesterase (AChE, EC 3.1.1.7, MAChE) using baculovirus and insect cell culture was constructed. The cDNA for AChE was synthesized from Drosophila melanogaster in Halla Mountain, the lipid anchor tail was removed by PCR and was used for the site-directed mutagenesis of three amino acid residues (E107Y, F368L, L408F). The mutated cDNA was inserted into the baculovirus vector and expressed in insect cells. Maximum cell growth and enzyme activity were reached when the cells $(2{\times}10^6\;cell/ml)$ were infected four times at four-day-intervals. His-tag containing MAChE was purified using Ni-NTA column and used for characterization. The activity was maintained under various pHs (3-10) and temperatures $(20-50^{\circ}C)$ under experimental conditions. As an extraction solution for pesticides, methanol is more effective than ethanol. Against major organophosphate and carbamate pesticides, the MAChE showed better sensitivity than AChE and AChE from housefly (Taiwan).

• The Journal of the Korea Contents Association
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• v.13 no.5
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• pp.286-294
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• 2013

The purpose of this study was to confirm the mental health and quality of life by type-D personality of the patients with coronary artery disease (CAD). The participants in the study were 111 hospitalized patients with CAD at a hospital in Gyeonggi-do. The type-D personality was assessed by the Type-D Personality Scale (DS14). The mental health was measured with Symptom Checklist-90-Revision (SCL-90R) while quality of life was assessed with World Health Quality of Life Assessment Instrument (WHOQOL-BREF). The data analysis revealed that 36.1% of CAD patients were identified as having type-D personality traits. Non type-D personality patients have shown better mental health state than type-D personality patients (p<.001) have. The level of quality of life in the type D personality patients were significantly lower than that of non type-D counterparts (p<.001). It is necessary for type-D personality to be considered, when the nursing intervention programs for improving the mental health and quality of life of the patients with CAD are developed.

• Korean Journal of Psychosomatic Medicine
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• v.10 no.2
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• pp.120-129
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• 2002

Objectives : The object of this study was to compare perceived stress and quality of life among patients with HIV infection, patients with pulmonary tuberculosis and normal controls Methods: Stress Response Inventory(SRI) and Symptom checklist 90-Revised(SCL-90-R) were used to measure perceived stress responses and psychopathology. Smithklein Beecham quality of life scale was used to measure quality of life. Results : Patients with HIV infection scored significantly higher on scores of tension, anger, depression, fatigue and frustration subscale of the SRI than those with pulmonary tuberculosis and normal controls. Scores of the SCL-90-R, somatization, depression, anxiety, hostility, phobic anxiety, paranoid ideation and psychoticism subscale were also significantly higher in patients with HIV infection than those with pulmonary tuberculosis and normal controls. Patients with HIV infection scored significantly lower in quality of life than those with pulmonary tuberculosis. In patients with HIV infection, age had a significantly negative correlation with scores of somatization, obsessive-compulsive, interpersonal sensitivity, phobic anxiety, paranoid ideation and psychoticism sub scale of the SCL-90-R. but the level of education had a significantly positive correlation with somatization, obsessive-compulsive, interpersonal sensitivity, phobic anxiety, paranoid ideation and psychoticism sub scale of the SCL-90-R. Conclusion : The results suggest that patients with HIV positive were likely to have higher levels of perceived stress response and psychopathology, and lower quality of life than those with pulmonary tuberculosis and normal controls.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.4
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• pp.472-480
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• 2018

This study was conducted to examine cognitive function and the ability for occupational participation in patients with mild and moderate dementia and to identify correlations between these two variables. The study investigated 95 dementia patients who visited a day care center in Daegu between September and November, 2017. Their detailed cognitive functions were examined using the Mini-Mental State Examination-Korean (MMSE-K) and the Korean Version of the Loewenstein Occupational Therapy Cognitive Assessment Geriatric population: LOTCA-G. Their occupational participation was measured using the Model of Human Occupation Screening Tool (MOHOST). The subjects' cognitive functions and abilities for occupational participation were analyzed using the descriptive statistics produced by the SPSS 20.0 program. In addition, Pearson correlation coefficient was used to analyze the correlations between the cognitive functions of the subjects and their abilities for occupational participation. The results of the study showed positive correlations between cognitive function and the ability for occupational participation of patients with mild and moderate dementia. Based on the results of this study, the evaluation of the patients with dementia and ability for occupational participation related to the cognitive function in establishing the therapeutic goal should be considered.

• The Journal of the Korea institute of electronic communication sciences
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• v.12 no.1
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• pp.219-228
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• 2017

The aim of this study was investigated the effect of cognitive training using tablet pc applications on cognitive function, daily living, and satisfaction in subacute stroke patients. This study participated in fourteen subacute stroke inpatients, in I general hospital, at located in Incheon. All subjects assigned that randomized each seven patients in experimental and control group. Two groups received to traditional cognitive therapy during half hour/day, 5 times per week, for 4 weeks. Experimental group performed additionally that cognitive training using tablet pc applications for 30 min/day. The outcome measures were the K-MMSE(: Korea-Mini Mental State Examination), MoCA-K(: Montreal Cognitive Assessments-Korea), MBI(: Modified Barthel Index), VAS(: Visual Analog Scale) for cognitive function, daily living, satisfaction. In results, Both group showed significant improvements after intervention in MoCA-K, MBI(p<.05). In comparison of change between two groups, experimental group showed significant improvements than control group in MoCA-K(p<.05). In comparison of satisfaction of two groups, both group was not significant difference(p>.05). we suggested that Cognitive training using tablet pc applications expected to positive effects the improvements of cognitive function in subacute stroke patients.

• Journal of Food Hygiene and Safety
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• v.19 no.4
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• pp.198-202
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• 2004

It was carried out to compare the relationship of EEC 4-plate test method and Charm II test method by HPLC. 46 only tetracyclines, 4 only sulfonamides, 4 sulfonamides+tetracyclines, one amphenicols+tetracyclines, and one amphenicols+sulfonarnides+tetracyclines, it was detected on 71 porks of EEC 4-plate positive or Charm II positive by HPLC. In case of sulfonamides and chloramphenicols, two test methods had related with each other, but had no relation in tetracyclines. Also, it was $10.5\%(6/57)$ tetracyclines, $81.8\%(9/11)$ chloramphenicols that were EEC 4 plate positive or Charm II positive, but not detectrd by HPLC. Consequently, EEC 4-plate test method and Charm II test method were suitable by screening test on antibiotics and sulfonamides etc.

• The Journal of Korean society of community based occupational therapy
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• v.6 no.2
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• pp.1-10
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• 2016

Objective : The purpose of this study was to examine the effect of multi-sensory stimulation training on cognitive function and balance skill of the community resident elderly. Methods : 10 participants who is over aged 65 years carry out multisensory stimulation training program for 12weeks, once a week for 60minutes. The multisensory stimulation training program is consisted of total 12 topic based on body schema, gross muscle and tactile stimulation. The result was measured using Mini-Mental State Examination-Korean and Berg Balance Scale to evaluate before and after of cognitive function and balance skill. And after intervention, the satisfaction of the program was investigated. Wilcoxon matched-pair signed rank test was used to compare pre- post difference of cognitive function and balance skill. Results : There was significant difference in cognitive function and balance skill(p<.05) and the satisfaction is positive at the end of the program. Conclusion : The multisensory stimulation training program has positive effects on cognitive function and balance skill for elderly. And the satisfaction is positive at the end of the program. The multisensory stimulation training program can enhance the satisfaction to elderly in the aging society. So, additional studies is needed for efficient use.

• The Journal of the Korea Contents Association
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• v.20 no.2
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• pp.534-546
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• 2020

The purpose of this study is to prove the positive effect of mind and body healing through the modified meditation method of Southern Buddhism as a systematic practice to concentrate or empty consciousness of the Buddhist temple in Dhammakaya, Thailand. To investigate the effects of the program on the participants, the experimental measurement tools in this study include the Immeasurable scale, spirituality scale, simple mental test scale, and blood pressure scale, pulse meter, thermometer, and recorder. When the measured values were compared with the corresponding t-test, there were some differences before and after the measurements based on the significance probability p <0.05. As a result, there was a significant correlation between spiritual support scale and the Immeasurable scale. In addition, the results of analyzing the data through interviews showed that the body and physiologically effective concentration was well after the meditation experience, and the intention to actively utilize this meditation method was confirmed. In this study, it was confirmed that there was a positive effect of the Samata practice method of Concentration meditation(CM), and thus the theoretical and experimental effects of intensive meditation were presented. I hope that these studies will accumulate and follow-up studies will be conducted through intensive meditation as a healing effect.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.10
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• pp.7078-7088
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• 2015

This study examined effects of health behaviors and sleep related factor on cognitive function in the elderly hypertensive patients. Data were collected through a face to face interview survey with structured questionnaire form 140 elderly with hypertension ($age{\geq}65years$) from February 5 to May 1, 2013. Research instruments included Pittsburgh Sleep Quality Index(PSQI), Epworth Sleepiness Scale(ESS) and Korean version the Mini-Mental State Examination(MMSE-K). Cognitive function was negatively related to degradation in quality of sleep(r=-.29, p<.001). Sleep duration were negatively related to body mass index(r=-.18, p=.032) and degradation in quality of sleep(r=-.59, p<.001). Sleep duration was positively related to daytime sleepiness(r=.22, p=.008). Hierarchical multiple regression showed that age, education levels and living arrangement were associated with cognitive function(F=8.56, p<.001, Adjusted $R^2=.14$). After controlling for demographic characteristics and health behaviors, degradation in quality of sleep(${\beta}=-.27$, p=.008) was identified as significant predictors of cognitive function. This final model explained 17.0% of the cognitive function in the elderly hypertensive patients(F=4.09, p<.001). Therefore, as a strategy improving cognitive function of the elderly with hypertension, therapeutic intervention should be developed to improve quality of sleep considering age, education levels and living arrangement.

• Therapeutic Science for Rehabilitation
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• v.9 no.4
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• pp.45-54
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• 2020

Objective : The purpose of this study was to identify the relationships among cognitive function, activities of daily living (ADL), participation and quality of life in community-dwelling elderly. Methods : Cognitive function, ADL, participation and quality of life were assessed in the 144 elderly people residing in the community. We used MMSE-K for measuring cognitive function, K-ADL for ADL, K-ACS for participation, and GQOL for quality of life. Results : Cognitive function was significantly correlated with social activity of participation and quality of life (physical health, psychological health, social relations and economic level). ADL was significantly correlated with participation (instrumental ADL, leisure activity) and all sub-areas of quality of life. Social activity of participation were significantly correlated with all subarea of quality of life except physical health. Among the variables, the most relevant variable was leisure activity. Conclusion : It was related to quality of life in the order of participation, ADL, and cognitive function. Among the sub-areas of participation, leisure activity was the most correlated with quality of life. This study, thus, aimed to provide basic data for successful elderly life by examining the correlation between cognitive function, daily life activity, participation and quality of life in community-dwelling elderly.