• The Korean Journal of Malacology
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• v.30 no.3
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• pp.219-226
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• 2014

The effects of different stocking densities on the growth and survival rate of the abalone, Haliotis discus hannai, were investigated in marine net cage for two years. Stocking density was set 15, 30, 45 and 60 percentage $(=per.)/m^2$ with share to cross-sectional area per shelter. The primary rearing period (PRP) and the secondary rearing period (SRP) were conducted by a year. One year mean water temperature of PRP and SRS showed the difference about $2^{\circ}C$. In the growth (initial mean shell length of abalone : $36.14{\pm}2.28mm$) of PRP, the absolute growth rate (ARG), daily growth rate (DGR) and specific growth rate (SGR) of the $15per./m^2$ were higher than those of density groups (P < 0.05). Survival rates of all density groups were showed no significant difference. In the growth (mean shell length of abalone : $55.26{\pm}6.93mm$) of SRP, ARG, DGR and SGR of stocking density groups showed no significant difference except for $45per./m^2$ density group. Survival rate in the low-density (15, $30per./m^2$) was more than 70%, and those of the high-density (45, $60per./m^2$) were less than 31% and 9%, respectively. These results showed that the appropriate stocking density for $15per./m^2$ was seven hundred fifty number per one net cage ($2.4{\times}2.4m$), during PRP using 3-4 cm abalone in length. Also for the secondary rearing period, the optimal stocking density (shell length 5-6 cm of abalone) consider with the economical efficiency was determined to be $30per./m^2$, resulting the productivity improved.

• The Korean Journal of Malacology
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• v.17 no.1
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• pp.45-55
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• 2001

Gametogenes, reproductive cycle, first sexual maturity(biological minimum size), sex ratio and larval development of the marsh clam Corbicula japonica were investigated monthly by histological observations. Samples were collected in brackish water of Gokgang stream, Kyungsangbuk-Do, Korea, from August 1997 to July 1998. Sexuality of Corbicula japonica is dioecious and the species are an oviparous clam. The gonads are irregularly arranged from the sub-region of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary is composed of a number of ovarian sac which are branched arborescent. Oogonia actively proliferate along the germinal epithelium of ovarian sac, in which young oocytes are growing. The testis is composed of a number of testicular tubules, and the epithelium of the tubule has function of germinal epithelium, along which spermatogonia actively proliferate. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed between developing oocytes and spermatocytes in the early developmental stages. With the further development of the ovary and testis these tissue and cells gradually disappear. Then the undifferentiated mesenchymal tissue and eosinophilic granular cells are considered to be related to the growing of the oocytes and spermatocytes. The spawning period is from July to September, and the main spawning occur between July and August when seawater temperatures reach above 22$^{\circ}C$. The reproductive cycle of this species can be divided into five successive stages; early active (February to April), late active (May to July), ripe (June to September), partially spawned (July to September), degenerative (September to October) and resting stage (October to February). Percentages of first sexual maturity of female and male clams ranging in length from 10 mm to 12 mm are over 50% and 100% for clams over 16.0 mm in shell length. Fertilized eggs or Corbicula japonica were 80-90 ${\mu}{\textrm}{m}$ in diameter. In the early embryonic development of C. japonica, the appearance of polar body, trochophore and D-shaped veliger were observed around 40 min., 27 hours and 4 days after spawning, respectively, at a water temperature of 26.5-28.$0^{\circ}C$. The size of larvae of early umbo stage was about 185-210 ${\mu}{\textrm}{m}$ in shell length, 160-180 ${\mu}{\textrm}{m}$ in shell height around 7 days after fertilization. The correlation of relative growth between the culture day (D) and shell length (SL) was expressed by the following simple formula from D-shaped veliger to metamorphosing stage; SL = 13.300D + 209.36($r^2$= 0.9078).

• The Korean Journal of Malacology
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• v.20 no.2
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• pp.125-130
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• 2004

Arkshell, Scapharca broughtonii spats were placed in natural environmental condition for 30 days from July 28 to August 27, 2004, in order to estabilish intermediate culture technique. Growth and survival of the spats in different intermediate culture areas with various culture methods were measured. Water temperature of studied area ranged from 24.1 to 28.5$^{\circ}C$, salinity was 15.4 to 33.3 psu, dissolved oxygen was 3.92 to 12.6 mg/l. Scapharca broughtonii spats cultured in Yeosu developed the best, 10.15 ${\pm}$ 1.12 mm in average shell length, and the highest survival was recorded as 77% in Namhae. Shell lengths of the Scapharca broughtonii spats cultured in the water depths of 2, 5, and 10 m were 7.14 ${\pm}$ 1.14 mm, 6.98 ${\pm}$ 1.74 mm and 6.27 ${\pm}$ 1.33 mm, and the survivals showed 75.5%, 77.0% and 76.5%, respectively. When 1 mm, 2 mm, and 3 mm-sized spats were cultured for 30 days in water depth of 5 m, the shell length increased to 6.73 ${\pm}$ 1.46 mm, 6.98 ${\pm}$ 1.74 mm and 7.04 ${\pm}$ 1.19 mm, and survivals were 67.0%, 77.0% and 58.5%, respectively. The shell lengths of spat cultured for 30 days in mesh sizes of 1 ${\times}$ 1 mm, 2 ${\times}$ 2 mm and 3 ${\times}$ 3 mm, 5 m below the surface were 8.14 ${\pm}$1.23 mm, 8.26 ${\pm}$ 1.19 mm and 8.78 ${\pm}$ 1.16 mm, and survivals were 41.5%, 43.0% and 44.5%, respectively.

• The Korean Journal of Malacology
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• v.16 no.1_2
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• pp.31-34
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• 2000

A series of rearing experiments have been conducted to determine the growth and survival rate in accordance with body size in closed recirculating sea water system. The abalone, Sulculus aquatilis, spat of 10.24${\pm}$0.85 mm, 0.36${\pm}$0.l g and 24.9${\pm}$1.07 mm 2.07${\pm}$0.57 g in shell length and body weight were used in this study Ranges of water temperature and pH during the rearing period of 90 days were 10.0-24.2$^{\circ}C$ and 7.8-8.2, respectively. Dissolved oxygen during the experiment period was 6.13-7.21 ml/l and inorganic nitrate was 0.68-3.72 $\mu\textrm{m}$ in NH$_4$$\^$+/-N, 0.17-7.79 fm in NO$_2$-N and 0.4-11.52 $\mu\textrm{m}$ in NO $_2$-N, Growth in shell length and body weight of the spat in large group and small group at the end of the experiment were 30.80${\pm}$3.14 mm, 17.98${\pm}$2.61 mm and 3.20${\pm}$0.8 g, 0.36${\pm}$0.1 g, and survival rate of spat in large group and small group at the end of the rearing experiment were 96.0% and 90.0% , respectively.

• The Korean Journal of Malacology
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• v.31 no.4
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• pp.263-266
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• 2015

This study was performed to determine the effect of water temperature condition on survival rate and growth of juvenile geoduck (Panopea japonica). Panopea japonica juveniles were bred for 6 weeks at 12, 15, 18, 21, 24 and $27^{\circ}C$ on incubator. Juveniles median lethal times (LT50) were 29 hours in $27^{\circ}C$, 14.5 day in $24^{\circ}C$, 37.4 day in $21^{\circ}C$. Survival rate of water temperature 12, 15 and $18^{\circ}C$ showed a high survival rate in 95.0%, 95.0% and 93.3% at 6 weeks. As a result of culturing for 6 weeks at 12, 15, 18, 21 and $24^{\circ}C$, a daily growth of shell length and total weight was 0.10 mm, 0.12 mm, 0.13 mm, 0.16 mm and 0.14 mm, and 2.21 mg, 2.65 mg, 2.84 mg, 3.13 mg and 2.93 mg. Juvenile shell length and total weight have significantly increased at $15-21^{\circ}C$. However, mortality rate has significantly increased at more than $21^{\circ}C$. As a result, appropriate water temperature for juveniles culturing considered $15-18^{\circ}C$.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.3
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• pp.210-221
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• 2000

The population habitat and density of Korean scallops, Chlamys farreri, were investigated to estimate population ecological characteristics from samples randomly collected around Wando from November, 1998 to October, 1999. Age and growth of the Korean scallops were determined from their ring radii. Maturation and spawning were studied using data of ovary maturity stage, gonadosomatic index, and fecundity. Seawater temperature and specific gravity ranged from 7.6 to $25.9^{\circ}C$ and from 1.0188 to 1.0260, respectively. Also dissolved oxygen and pH ranged from 6.48 to 9.50 ppm and from 8.17 to 8.80. Rocky and gravel bottom had a maximum habitat density of $$28.83 inds/100m^2$$ , which accounted for 82.4 % of the overall habitat area. The relationship between shell length (SL, mm) and shell height (SH, mm) of the Korean scallops was fitted : SH=1.021 SL+2.211 $(R^2=0.989)$. The shell length-total weight (TW, g) relationship was $TW=0.0003; SL^{2.837};(R^2=0.980)$. Then von Bertalanffy growth parameters were estimated from a nonlinear regression method, and the values were as follows : $SH_{\infty}=117.4 mm$K=0.61/year,; and; t_0=-0.017 year., The 50 % maturity at age was 0.21 year with the shell height of 18.3 mm, and spawning occurred twice a year, that is, June/July and October. The relationship between fecundity (Fc) and shell length was$Fc=697.03 SL^{2.683}(R^2=0.984)$, and the fecundity-gonad weight (GW, g) relationship was Fc=10,076,090 GW+15,608,781 $(R^2=0.990)$.

• Development and Reproduction
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• v.9 no.2
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• pp.123-134
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• 2005

Germ cell development during oogenesis, ovarian maturation and first sexual maturity in female Ruditapes philippinarum were investigated by cytological and histological observations. R. philippinarum is dioecious. During vitellogenesis, the Golgi complex, glycogen particles, and mitochondria were involved in the formation of lipid droplets and lipid granules in the cytoplasm of the early vitellogenic oocyte. In the late vitellogenic oocyte, cortical granules, the endoplasmic reticulum, and mitochondria were involved in the formation of proteid yolk granules in the cytoplasm. At this time, exogenous lipid granular substance and glycogen particles in the germinal epithelium passed into the oocyte through the microvilli of the vitelline envelope. The spawning period was once a year between early June and early October, and the main spawning occurred between July and August when seawater temperature was approximately $20^{\circ}C$. The reproductive cycle of this species can be categorized into five successive stages: early active stage(January to March), late active stage(Februaryto May), ripe stage(April to August), partially spawned stage(May to October), and spent/inactive stage (August to February). Percentages of female clams at first sexual maturity of $15.1{\sim}20.0mm$ in shell length were 52.6%(50% of the rate of group maturity was 17.83mm in length), and 100% for the clams >25.1mm.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.291-297
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• 2011

In an attempt to improve the substrate condition for Manila clam (Ruditapes philippinarum) culture, crushed oyster (Crassostrea gigas) shells were spread on the muddy tidal flat of Namseong-ri, Podu-myeon, Goheung-gun, Jeollannam-do in April 2008. To test the suitability of the crushed oyster shell added substrate, seed clams were transplanted from Taehwa river estuary in Ulsan city in June 2008. Over 23 months of sampling, the mean grain size and the sorting in the experimental site containing the crushed oyster shell were significantly higher than the control site. The ignition loss, water content, chemical oxygen demand (COD) and acid-volatile sulfide (AVS) level were also significantly higher in the crushed oyste shell added substratum. Survival of the clams transplanted to the crushed oyster shell added substratum was significantly higher and all the clams transplanted to the normal muddy substratum died in August 2009, 13 months after the transplantation. At the end of the experiment in April 2010, the transplanted clams reached 36.10 mm in shell length and 8.92 g in total weight with survival of 43.5%. Our study suggested than crushed oyster shell added in the mud dominant substratum greatly improved living condition and survivability of clams.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.121-127
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• 2013

In this study, growth-line analysis was carried out on the hard clam (Meretrix petechialis) collected from the Neolithic shell middens in Daejuk-ri, Seosan, Korea, to reconstruct palaeoenvironment. Growth increments of 206 specimens of the clam were examined. The ages of the specimens were determined from the rings on the shells. The relationship between shell length and ring radius in each ring group was expressed as a regression line, indicating a correspondence in each ring formation. Growth pattern of the midden specimens was compared to that of modern ones collected from Gimje, Jeonbuk. Growth curves for shell length fitted to the von Berta anffy's growth curve were expressed respectively as follows: $SL_t=102.9025[1-e^{-0.18657(t+1.0906)}]$ in the shell midden specimens, $SL_t=104.2583[1-e^{-0.2277(t+0.7499)}]$ in the modern ones. The relationship between shell length (SL; mm) and shell height (SH; mm) was expressed by the following equations: SH = 0.7791 SL + 3.6636 ($R^2$ = 0.946) in the midden specimens, SH = 0.8103 SL + 0.5145 ($R^2$ = 0.991) in the modern ones. The results of the tests regarding the differences between regression coefficients and elevations of growth curves of these two populations demonstrate that the slopes were not significantly different (p < 0.05), but the elevations were (p > 0.05). However, overall growth curves of the midden and modern populations were not significantly different, indicating that shell growth environments of the two areas are similar. Therefore, it is likely that sea temperature near the midden area could be similar to that of present Gimje area, and thus temperature during the period of the midden formation could be higher than presently known.

• The Korean Journal of Malacology
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• v.28 no.4
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• pp.335-342
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• 2012

This study was conducted to estimation the selection response for growth-related traits in 31-month old of Pacific abalone, Haliotis discus hannai were born in 2008. In overall mean of surveyed traits and standard deviation showed $76.31{\pm}7.247$ mm of shell length, $49.48{\pm}5.307$ mm of shell width and $40.96{\pm}6.80$ g of total weight. The effect of sex and maturity were statistically significant in all traits include out a condition factor (p < 0.01). And In effect of sex, female were higher than male in all traits excepting condition factor, significantly. In correlation between breeding value and phenotypic value by each traits, showed rank correlation coefficient and simple correlation coefficient, which represented low positive correlation. A hopeful genetic improvement to the next generation showed 6.96 mm of shell length, 4.47 mm of shel width and 12.93 g of total weight. Therefor, It is considered that if considering properly selection intensity and selection ratio, efficient improvement could be made.