• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.326-329
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• 2004

Corynebacterium acetoacidophilum RYU3161 was cultivated in a L-histidine-limited fed-batch culture. To investigate the effect of cell growth on the L-proline production, 5 L fed-batch culture was performed using an exponential feeding rate to obtain the specific growth rates $(\mu)$ of 0.04, 0.06, 0.08, and 0.1 $h_1$. The results show that the highest production of L-proline was obtained at $\mu$ = 0.04 $h_1$. The specific L-proline production rate $(Q_p)$ increased pro-portionally as a function of the specific growth rate, but decreased after it revealed the maxi-mum value at $\mu$ = 0.08 $h_1$. Thus, the highest productivity of L-proline was 1.66 g $L^-^1 h^-^1$ at $\mu$ = 0.08 $h_1$. The results show that the production of L-proline in C. acetoacidophilum RYU3161 has mixed growth-associated characteristics.

• Journal of Ecology and Environment
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• 제37권3호
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• pp.113-122
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• 2014

The purpose of this study is to compare soil $CO_2$ efflux between burned and unburned sites dominated by Pinus densiflora forest in the Samcheok area where a big forest fire broke out along the east coast in 2000 and to measure soil $CO_2$ efflux and environmental factors between March 2011 and February 2012. Soil $CO_2$ efflux was measured with LI-6400 once a month; the soil temperature at 10 cm depth, air temperature, and soil moisture contents were measured in continuum. Soil $CO_2$ efflux showed the maximum value in August 2011 as 417.8 mg $CO_2m^{-2}h^{-1}$ (at burned site) and 1175.1 mg $CO_2m^{-2}h^{-1}$ (at unburned site), while it showed the minimum value as 41.4 mg $CO_2m^{-2}h^{-1}$ (at burned site) in December 2011 and 42.7 mg $CO_2m^{-2}h^{-1}$ (at unburned site) in February 2012. The result showed the high correlation between soil $CO_2$ efflux and the seasonal changes in temperature. More specifically, soil temperature showed higher correlation with soil $CO_2$ efflux in the burned site ($R^2$ = 0.932, P < 0.001) and the unburned site ($R^2$ = 0.942, P < 0.001) than the air temperature in the burned site ($R^2$ = 0.668, P < 0.01) and the unburned site ($R^2$ = 0.729, P < 0.001). $Q_{10}$ values showed higher sensitivity in the unburned site (4.572) than in the burned site (2.408). The total soil $CO_2$ efflux was obtained with the exponential function between soil $CO_2$ efflux and soil temperature during the research period, and it showed 2.5 times higher in the unburned site (35.59 t $CO_2ha^{-2}yr^{-1}$, 1 t = $10^3$ kg) than in the burned site (14.69 t $CO_2ha^{-2}yr^{-1}$).