• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.6
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• pp.1092-1096
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• 2002

Ethyl acetate and butanol fractions among the serial solvent fractions of grape seed ethanol extract contained the catechin at the levels of 35.7 mg/g and 20.2 mg/g, respectively However, the POV increasing patterns of two linoleic acid samples containing each solvent fraction were so similar that the difference in antioxidant activity by the catechin content of each solvent fraction could not be found. Each solvent fraction was fractionated on C18 cartridges into three subfractions which were mono-, dimers fraction (FI), oligomers fraction (FII) and polymers fraction (FIII) to examine the effect by the difference in degree of Polymerization of proanthocyanidin. The catechin contents of ethyl acetate subfractions (E-F) were in the order of E-FI (26.0 mg/g) > E-FII (18.6 mg/g) > E-FIII (13.7 mg/g) but the three subfractions showed nearly similar antioxidant activities, by the POV measurement at 1,000 ppm concentration. Also the catechin contents of butanol subfractions (B-F) were in the order of B-FI (35.3 mg/g) > B-FII (30.8 mg/g) > B-FIII (22.7 mg/g) but similar antioxidant activities were observed in all subfractions. In this study, similar antioxidant activities of each solvent subfraction in spite of different catechin contents inform that the degree of polymerization of proanthocyanidin as well as the total catechin content should be considered in quality control of grape seed extract produced for natural antioxidant.

• Journal of Korean Society of Forest Science
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• v.95 no.4
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• pp.429-434
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• 2006

The dried needles (1.5 kg) of Picea abies Karsten were ground, extracted with acetone-$H_2O$ (7:3, v/v), concentrated, and fractionated with a series of n-hexane, methylene chloride, ethyl acetate and water on a separatory funnel. Each fraction was freeze dried, then a portion of ethyl acetate soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-n-hexane mixture as eluents. The isolated compounds were identified by cellulose TLC, $^1H$-, $^{13}C$-NMR, FAB and EI-MS. (+)-catechin (compound I), (-)-epicatechin (compound II), kaempferol-3-O-${\beta}$-D-glucopyranoside (compound III), 4-hydroxyacetophenone (compound IV) were isolated from the ethyl acetate soluble fraction and (+)-catechin (compound I), protocatechuic acid (compound V) were isolated from the $H_2O$ soluble fraction of P. abies needle. The antioxidative activities of each fraction and the isolated compounds were tested by DPPH radical scavenging method, and EtOAc soluble fraction, (+)-catechin and (-)-epicatechin showed similar values to ${\alpha}$-tocopherol and BHT as controls.

• Journal of the Korean Wood Science and Technology
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• v.30 no.1
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• pp.63-71
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• 2002

The bark of P. alba × glandulosa, P. euramericana and P. nigra × maximounczii F₁, several Populus trees, were collected, extracted with acetone-H₂O(7:3, v/v), fractionated with hexane, chloroform and ethylacetate, and freeze dried to give some dark brown powder. Each fraction of the powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents and then identified by thin layer chromatography using TBA and 6% acetic acid as developing solvents. The structures of the isolated compounds were characterized by ¹H, ¹³C and 2D-NMR tools including mass spectrometry. Most of the compounds were flavonoids and salicin derivatives as follows: (+)-catechin, taxifolin, aromadendrin, eriodictyol, naringenin, sakuranetin, sakuranetin-5-O-𝛽-D-glucopyranoside, neosaturanin, salireposide, p-coumaric acid, and aesculin from P. alba × glandulosa, (+)-catechin, salireposide, populoside and salicortin from P. euramericana and (+)-catechin, quercetin, padmatin, salireposide, populoside and salicortin from P. nigra × maximounczii F₁.

• Journal of Life Science
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• v.34 no.1
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• pp.59-67
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• 2024

LS-RUG-com preparation in a complex extract from mixture of three natural plants. Rubus crataegifolius/unriped, Gardenia jasminoides and Ulmus marcrocarpa that have been widely used in traditional functional health food. This study was conducted to establish the HPLC analysis methods that can be used to establish quantitative analysis of R. crataegifolius, G. jasminoides and U. macrocarpa for standardization of LS-RUG-com preparations. HPLC analysis methods for simultaneous determination of ellagic acid and geniposide and single determination of catechin-7-O-β-D-apiofuranoside were established for the quality control of natural plants complex (LS-RUG-com). Validation of HPLC analysis were performed by checking specificity, accuracy, precision, limit of detection and quantitation, and linearity following ICH (International Council for Harmonisation) guideline. As the result of quantitative analysis, the contents of ellagic acid, geniposide and catechin-7-O-β-D-apiofuranoside in each plant extracts were 11.2 mg/g (ellagic acid) and 72 mg/g (geniposide) and 10.2 mg/g (catechin-7-O-β-D-apiofuranoside). The contents of ellagic acid, geniposide and catechin-7-O-β-D-apiofuranoside in LS-RUG-com were 4.62~6.82 mg/g (ellagic acid), 19.2~28.8 mg/g (geniposide) and 1.36~2.04 mg/g (catechin-7-O-β-D-apiofuranoside) respectively.

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.05a
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• pp.105-105
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• 2003

본 연구는 쇠고기 분쇄육에 천연항산화제인 pycnogenol, catechin 및 $\alpha$-tocopherol과 합성항산화제인 BHT를 처리한 후 3, 5, 7, 10일 동안 냉장저장하면서 색도 및 pH, TBA 값 및 지방산 조성의 변화와 항산화효소인 SOD 및 catalase 활성의 변화를 검토하였다. 명도는 catechin 처리군이 저장에 따른 색의 변화가 가장 작았으며, pH는 처리 당일 5.83~5.87의 비슷한 수준이었으나 저장함에 따라 저하되어 5일째에는 대조군이 약 0.45 수준으로 크게 감소한 것에 비하여 항산화제처리군은 약 0.2 수준의 감소로 대조군과 큰 차이를 나타냈다. (중략)

• Korean Journal of Plant Resources
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• v.10 no.1
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• pp.6-10
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• 1997

From the leaf of Prunus davidiana, naringenin and its glucoside, kaempferol and its glucoside, kaempferide glucoside, quercetin glucoside and d-catechin were isolated.

• Korean Journal of Pharmacognosy
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• v.27 no.3
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• pp.219-223
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• 1996

From the rachis of Cedrela sinensis A. Juss., methyl gallate, quercitrin, bis-(p-hydroxyphenyl)ether, adenosine, isoquercitrin, rutin, (+)-catechin and (-)-epicatechin were isolated and characterized by spectral data.

• Korean Journal of Pharmacognosy
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• v.23 no.3
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• pp.121-125
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• 1992

Three compounds were isolated from the chloroform and n-butanol extracts of Filipendula glaberrima (Rosaceae). The structures of these isolates were elucidated as monotropitin, (+)-catechin, and ${\beta}-sitosteryl-3-O-{\beta}-D-glucopyranoside$ by spectroscopic analysis, and were identified by comparison of their spectra with those of reported ones.

• Bulletin of the Korean Chemical Society
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• v.33 no.12
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• pp.4235-4238
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• 2012

• Korean Journal of Pharmacognosy
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• v.36 no.3 s.142
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• pp.191-194
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• 2005

Four compounds were isolated from the twigs of Vaccinium oldhami (Ericaceae) through repeated column chromatography. Their chemical structures were elucidated as (+)-catechin, (-)-epicatechin, proanthocyanidin A-2, and cinnamtannin $B_1$, respectively, by spectroscopic analysis.