• 임산에너지
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• 제21권1호
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• pp.41-48
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• 2002

참나무속 상수리나무와 굴참나무 수피를 채취하여 아세톤-물(7:3, v/v) 혼합용액으로 추출하고 유기용매를 제거한 후 hexane, CH₂C1₂, EtOAc 및 수용성으로 분획하여 동결건조하였다. 두 수종의 EtOAc용성 분획물은 MeOH 수용액 및 EtOH-hexane 혼합용액을 사용하여 Sephadex LH-20 칼럼크로마토그래피를 수행하였다. 단리된 화합물의 구조는 ¹H, /sup 13/C 및 2D-NMR spectrum으로 구조를 구명하였다. 화합물의 분자량은 FAB-MS로 측정하였다. 굴참나무에서는 (+)-catechin, caffeic acid, taxifolin-3-O-β-D-glucopyranoside를 단리 하였으며 상수리나무에서는 (+)-catechin, (+)-gallocatechin, gallic acid, taxifolin-3-O-β-D-glucopyranoside를 단리 하였다.

• Bulletin of the Korean Chemical Society
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• 제28권2호
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• pp.276-280
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• 2007

In this work, caffeine and some catechin compounds + C, EC, EGC, and EGCG were extracted from green tea by using molecular imprinted polymers (MIP) as sorbent materials in a solid-phase extraction (SPE) process known as MISPE (molecular imprinted solid-phase extraction). For synthesis of MIP, caffeine was employed as the template, MAA as the monomer, EGDMA as the crosslinker, and AIBN as the initiator. A solution of caffeine (0.2 mg/mL in methanol) was utilized in the solid extraction cartridges following loading, washing, and elution procedures with acetonitrile, methanol, and methanol-acetic acid (90/10, %v/v) as the solvents, respectively. This solid-phase extraction protocol was applied for the extraction of caffeine and some catechin compounds from green tea. A comparison was made between the results obtained with the MIP cartridges and a traditional C18 reversed-phase cartridge. It was thereupon found that the recovery of caffeine by the MIPbased sorbent used in this work was almost two and four times greater than that by a commercially available C18 material. A quantitative analysis was conducted by high performance liquid chromatography (HPLC) using a C18 column (5 μm, 250 × 4.6 mm) with methanol/water (40/60, %v/v) as the mobile phase at a flow rate of 0.5 mL/min.

• 대한화학회지
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• 제65권2호
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• pp.106-112
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• 2021

12 kinds of (-)-catechin derivatives were designed and synthesized. The catechin derivatives were evaluated their antioxidant activities using DPPH method. Most of them showed good antioxidant activity, particularly compounds 1d, 1e and 1j exhibited more activity than butylated hydroxytoluene (BHT). Molecular docking studies for compounds 1d, 1e and 1j with STAT1 showed not only sufficent characteristics binding cavity but also agreement with the observed biological activity. Acording to docking results, the compounds showed greater than hydrogen bonding, hydrophobic interactions, electrostatic interactions, and Van der Waals interactions as compared to the reference compound. They formed hydrogen bonds with important residues such as Lys566, His568, Leu570, and Phe644. The compounds showed a novel hydrogen bonding interaction with Arg649, which was not reported previously. Our results might suggest the compounds could serve as a novel anti-oxidant agent.

• 대한화학회지
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• 제51권2호
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• pp.165-170
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• 2007

고정상 추출에 케르세틴 분자각인 고분자를 흡착제로 하여 녹차에서 카페인과 +C, EGC, EGCG와 같은 카테킨 화합물을 추출하였다. Quercetin을 주형분자로, MAA를 단량체로, EGDMA를 가교제로 하고 AIBN을 개시제로 하여 MIP를 합성하였다. 녹차에서 카페인과 카테킨 화합물을 추출하기 위하여 고정상 추출에서의 주입, 세척, 용출용매로 각각 물, 메탄올, 메탄올:아세트산=90:10(vol.%)을 사용하였다. 고성능 액체 크로마토그래피 분석조건은 C18 컬럼(5 μm, 250×4.6 mm, RS-tech 회사), 메탄올/물(40/60, vol.%)을 이동상 조건으로하고 유속은 0.5 ml/min으로 하였다. 분자각인 고정상 추출을 통과함으로써, 카페인과 카테킨 화합물의 분리도는 증가하였다. 또한 케르세틴 분자각인 고분자는 생성된 공극 구조와 유사한 화학적구조식을 가진 +C화합물에 대하여 더 우수한 선택성을 가졌다.

• 생명과학회지
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• 제18권2호
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• pp.273-278
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• 2008

콜레스테롤 생합성 과정에 있어서 속도조절 단계 효소의 하나인 squalene synthase에 대한 저해물질의 탐색을 목적으로, 30종의 다양한 천연물을 대상으로 squalene synthase에 대해 저해효과를 검토한 결과 녹차추출물에서 비교적 저해활성이 높고 재현성이 있게 저해효과를 나타내는 것으로 확인되었다. 녹차에 함유되어 있는 squalene synaase에 대한 저해물질의 용매추출성을 검토한 결과 ethyl acetate와 n-butanol 층에 저해물질이 많이 함유되어 있는 것으로 확인되었으며 저해물질은 녹차의 polyphenol 화합물인 catechin에 의한 것으로 추정되었다. Catechin 표준용액의 각 농도에 따른 squalene synthase 저해작용을 살펴 본 결과, (-)-epigallocatechin gallate, (-)-epicatechin gallate, (-)-epigauocatechin, (-)-epicatechin, (+)-catechin의 순으로 저해활성이 강한 것으로 나타났으며 가장 강한 저해활성을 나타내는 (-)-epigallocatechin gallate의 $IC_{50}$값은 $90{\mu}M$이었다.

• 대한화장품학회지
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• 제35권3호
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• pp.251-256
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• 2009

개가시나무 가지의 에탄올 추출물에서 항산화활성을 관찰하였으며, 활성성분을 규명하기 위한 연구를 진행하였다. 그 결과, 4종류의 화합물을 분리하여 동정하였다. 분리 동정된 성분은 catechin(1), epi-catechin(2), tyrosol(3) 및 tiliroside(4)이다. 분리 성분의 항산화활성은 DPPH 라디칼 및 superoxide 음이온 라디칼 소거활성을 이용하여 측정하였다. 화합물 1, 2, 3, 4는 $100{\mu}L$/mL 농도에서 각각 94.2 %, 93.4 %, 33.6 %, 11.2 %의 DPPH 라디칼 저해활성을 나타내었다. 또한, 화합물 1, 2, 3, 4는 $200{\mu}L$/mL 농도에서 각각 60.2 %, 35.1 %, 20.6%, 4.5 %의 superoxide 음이온 라디칼 저해활성을 나타내었다. 화합물 $1{\sim}4$는 개가시나무에서는 처음으로 분리된 물질이다.

• 한국콘텐츠학회논문지
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• 제12권1호
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• pp.338-345
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• 2012

본 연구는 비만인 여성을 대상으로 카테킨 음료 음용이 체중, 총콜레스테롤, 중성지방, 고밀도 지단백, 저밀도 지단백에 미치는 효과를 규명하여 운동 보조제로서의 카테킨의 효용성을 검정하는데 연구의 목적이 있다. 비만여성 총 27명을 대상으로 차카테킨 음료음용 집단 8명, 운동적용 집단 9명, 차카테킨 음료 음용 및 운동 병행 집단 10명을 분류하여 카테킨섭취와 운동을 실시한 후 혈중지질 요인을 측정하여 효과를 분석한 결과 체중, 중성지방, 저밀도 지단백 콜레스테롤 농도는 음료섭취와 운동 병행집단(p<0.001)과 운동집단(p<0.001)만이 유의하게 감소하였으며, 총 콜레스테롤은 세집단 모두 유의하게(p<0.05) 감소하였다. 비만 여성의 체중감소와 혈중지질 성분의 저하를 위해서는 카테킨 성분의 음료와 운동을 병행할 경우 효과를 극대화시킬 수 있을 것으로 보여진다.

• 생약학회지
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• 제47권3호
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• pp.246-250
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• 2016

Pinus densiflora contained diverse phenol compounds like flavonoid, phenylpropanoid and tannin. PineXol$^{(R)}$ is nutraceutical preparation which was treated from bark of Pinus densiflora. Validation and contents determination of taxifolin, (+)-catechin and procyanidion B1 for the preparation of Pinus densiflora (PineXol$^{(R)}$) were confirmed using High-Performance Liquid Chromatography (HPLC). As a result, content of taxifolin, (+)-catechin and procyanidin B1 were, respectively 4.90%, 2.35% and 8.19%. These analysis method and results could be used as important basic data for the preparation of Pinus densiflora.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.1011-1021
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• 2021

The root bark of Ulmus davidiana var. japonica (Japanese elm) is used in Korea and other East Asian countries as a traditional herbal remedy to treat a variety of inflammatory diseases and ailments such as edema, gastric cancer and mastitis. For this study, we investigated the lipid metabolism and anti-obesity efficacy of ethyl alcohol extract of Ulmus davidiana var. japonica root bark (UDE). First, HPLC was performed to quantify the level of (+)-catechin, the active ingredient of UDE. In the following experiments, cultured 3T3-L1 pre-adipocytes and high-fat diet (HFD)-fed murine model were studied for anti-obesity efficacy by testing the lipid metabolism effects of UDE and (+)-catechin. In the test using 3T3-L1 pre-adipocytes, treatment with UDE inhibited adipocyte differentiation and significantly reduced the production of adipogenic genes and transcription factors PPARγ, C/EBPα and SREBP-1c. HFD-fed, obese mice were administered with UDE (200 mg/kg per day) and (+)-catechin (30 mg/kg per day) by oral gavage for 4 weeks. Weight gain, epididymal and abdominal adipose tissue mass were significantly reduced, and a change in adipocyte size was observed in the UDE and (+)-catechin treatment groups compared to the untreated control group (^***p < 0.001). Significantly lower total cholesterol and triglyceride levels were detected in UDE-treated HFD mice compared to the control, revealing the efficacy of UDE. In addition, it was found that lipid accumulation in hepatocytes was also significantly reduced after administration of UDE. These results suggest that UDE has significant anti-obesity and lipid metabolism effects through inhibition of adipocyte differentiation and adipogenesis.

• 생약학회지
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• 제51권4호
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• pp.291-296
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• 2020

Filipendula glaberrima (FG) is a plant endemic to South Korea. It is economically important as a food source and used as a medicine in treating ailments. Filipendula flowers are characterized by the presence of several polyphenolic constituents. The aim of this study is to determine the content of (+)-catechin in Filipendula glaberrima collected from different regions at different flowering stages. High-performance liquid chromatography with a gradient elution system (0.5% acetic acid in water : acetonitrile = 95 : 5 to 0 : 100 for 35 min) was used. A reverse-phase INNO column with UV detection at 278 nm was employed. The results revealed that F. glaberrima from Mt. Odae has the highest (+)-catechin content (10.600 mg/g). Furthermore, its content was the lowest in samples collected during the pre-flowering period and the highest at the early-flowering stage. This study provides a basis in establishing the optimal period and the best region for collecting F. glaberrima with maximized (+)-catechin yield.