• 터널과지하공간
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• 제11권3호
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• pp.217-224
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• 2001

암석은 지질학적 생성과정으로 인해 많은 역학적 결함을 포함하고 있으며 이러한 결함 사이에는 암석 브릿지가 존재하게 된다. 이러한 암석 브릿지에서의 균열의 전파 및 결합(coalescence)과정은 사면, 기초, 터널 등의 안정성에 영향을 미치게 된다. 본 연구에서는 단축압축 하에서 균열의 형상변화에 따른 암석 브릿지에서의 균열의 개시, 전파 및 결합거동 변화에 대해 알아보았다. 여산 대리석을 재료로 120$\times$60$\times$25 mm크기의 시료에 균열각도 $\alpha$, 브릿지각도 $\beta$, 균열길이 2c, 브릿지길이 2b를 변화시키면서 2개의 인공균열을 제작하였다. 하중을 가하면서 날개형 균열개시응력, 날개형 균열 전파각도, 균열결합 응력을 측정하였으며 균열결합 유형을 정리하였다. 또한, 정규화된 최대강도(normalized peak strength)를 구하여 Ashby & Hallam 모형 (1986)의 이론해와 비교, 분석 하였다.

• 한국정보과학회논문지:소프트웨어및응용
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• 제29권11호
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• pp.837-850
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• 2002

본 논문에서는 인지적 긍정/부정 선택 과제의 수행 시 뇌파를 이용하여 피험자의 반응시간 RT(response time)를 예측하는 방법에 관하여 기술한다. 실험 task에서 피험자는 시각적 자극에 대한 반응, 문제의 해석, 손 움직임의 조절, 손동작 등과 관련된 뇌활동을 한다. 이와 같은 피험자의 정신상태의 변화를 CT(cut time), ST(selection time), RP(repeated period) 등을 정의하여 모델링하고, 선택시간 ST를 감지하여 피험자의 반응시간 RT를 예측한다. ST를 감지하기 위하여 측정한 뇌파로부터 $\alpha$, $\beta$, ${\gamma}$파를 분리하고, 공간적인 관계를 고려하여 설정한 4쌍의 전극들로부터 3가지의 특징들을 추출한다. 추출한 특징들을 분석하여 각 피험자별로 나타나는 상세 규칙(specific rule)과 공통적인 특징들로 구성된 일반 규칙(meta rule)들을 설정한다. 8명의 피험자를 대상으로 설정한 규칙들을 적용하여 평균 83%의 ST 감지 성공률을 보이고, ST 감지 이후 약 0.73초에서 RT가 나타나는 것을 보인다. 설정한 규칙들의 타당성을 검증하기 위하여 8명 중 2명의 피험자에 대해서 재실험을 하고, 이들 데이타에 적용한 결과를 보인다. 본 논문에서 제안한 방법을 기존의 인지적인 정신상태 판별을 위한 방법들이나 왼손/오른손 동작구분 방법들과 결합하여 사용할 경우 BCI를 위한 기반 기술로 활용될 것으로 기대한다.

• 한국표면공학회지
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• 제41권2호
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• pp.57-62
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• 2008

Electrochemical properties of Ti-30Ta-$(3{\sim}15)$Nb alloys coated by HA/Ti compound layer have been studied by various electrochemical method. Ti-30Ta binary alloys contained 3, 7, 10, and 15 wt% Nb contents were manufactured by the vacuum furnace system. The specimens were homogenized for 24 hrs at $1000^{\circ}C$. The samples were cut and polished for corrosion test and coating. It was coated with HA/Ti compound layer by magnetron sputter. The HA/Ti non-coated and coated morphology of Ti alloy were analyzed by x-ray diffractometer(XRD) and filed emission scanning electron microscope(FE-SEM). The corrosion behaviors were investigated using potentiodynamic method in 0.9% NaCl solution at $36.5{\pm}1^{\circ}C$. The homoginazed Ti-30Ta-$(3{\sim}15wt%$)Nb alloys showed the ${\alpha}+{\beta}$ phase, and ${\beta}$ phase peak was predominantly appeared with increasing Nb content. The microstructure of Ti alloy was transformed from needle-like structure to equiaxed structure as Nb content increased. HA/Ti composite surface showed uniform coating layer with 750 nm thickness. The corrosion resistance of HA/Ti composite coated Ti-alloys were higher than those of the non-coated samples in 0.9% NaCl solution at $36.5{\pm}1^{\circ}C$. Especially, corrosion resistance of Ti-Ta-Nb system increased as Nb content increased.

• Journal of Acupuncture Research
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• 제30권2호
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• pp.31-41
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• 2013

Objectives : This study was to evaluate inhibitory effects of Naegwan-acupuncture($PC_6$) on acute RE(reflux esophigitis) rat induced by pylorus and forestomach ligation operation. Methods : Twenty seven SD rats were divided three groups (intact normal rat; RE control rat; RE control rat respectively stimulated by Naegwan point($PC_6$)). All rats was fasted for 18 h but free water, we induced RE by pylorus and forestomach ligation operation. Six hour after the operation, rats were sacrified, collected bloods in the abdominal vein, dissected a esophagus and stomach. The stomach was washed a 1 ml PBS to research gastric volume, pH, acidity and mucin release of gastric juice, esophagus was cut longitudinally and pictured a innter mucosa area to research damages in esophagus. The proinflammatory cytokine and chemokine including IFN-${\gamma}$, TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and MCP-1 were analyzed by ELISA kit. Results : 1. Significantly, death rate of $PC_6$ acupuncture rat group was decreased compared to that of RE control group. 2. Gastric Volume, gastric injury and esophageal mucosa demage were decreased significantly, too. 3. Compared with RE, all of the proinflammatory cytokine and chemokine analyzed in serum of $PC_6$ were decreased remarkably. Especially, there were significant meanings TNF-${\alpha}$, IL-6 and MCP-1 in serum of $PC_6$ were decreased. Conclusion : The results suggest that antiinflammatory and protecting effects of PC6 could attenuate the severity of reflux esophagitis and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease.

• 한국재료학회지
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• 제20권11호
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• pp.559-569
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• 2010

The liquid-phase sintering method was used to prepare a glass lens forming core composed of SiC-$Al_2O_3-Y_2O_3$. Spark plasma sintering was used to obtain dense sintered bodies. The sintering characteristics of different SiC sources and compositions of additives were studied. Results revealed that, owing to its initial larger surface area, $\alpha$-SiC offers sinterability that is superior to that of $\beta$-SiC. A maximum density of $3.32\;g/cm^3$ (theoretical density [TD] of 99.7%) was obtained in $\alpha$-SiC-10 wt% ($6Al_2O_3-4Y_2O_3$) sintered at $1850^{\circ}C$ without high-energy ball milling. The maximum hardness and compression stress of the sintered body reached 2870 Hv and 1110 MPa, respectively. The optimum ultra-precision machining parameters were a grinding speed of 1243 m/min, work spindle rotation rate of 100 rpm, feed rate of 0.5 mm/min, and depth of cut of $0.2\;{\mu}m$. The surface roughnesses of the thus prepared final products were Ra = 4.3 nm and Rt = 55.3 nm for the aspheric lens forming core and Ra = 4.4 nm and Rt = 41.9 for the spherical lens forming core. These values were found to be sufficiently low, and the cores showed good compatibility between SiC and the diamond-like carbon (DLC) coating material. Thus, these glass lens forming cores have great potential for application in the lens industry.

• Clinical and Experimental Reproductive Medicine
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• 제26권1호
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• pp.1-8
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• 1999

In order to study the implantation mechanism various methods for culture of endometrial cells in vitro have been attempted. However, a disadvantage is that primary cultures of stromal and epithelial cells do not have the ability to differentiate, and therefore cannot be reproduced in the same manner as in vivo endometrium. The object of this study is to establish a three dimensional culture of endometrial cells which are both morphologically and functionally identical to in vivo endometrium. Endometrial tissues obtained after hysterectomies were cut into thin slices and treated with collagenase and trypsin-EDTA. The stromal cells and the epithelial cells were separated by centrifugation and cultured for 24 hours in DMEM media containing 10% FCS, 100 nM progesterone, and 1 nM estradiol. The cultured stromal cells were mixed with collagen gel and solidified, after which it was covered with matrigel. Epithelial cells were inoculated on the top and then cultured for 3 days. The three dimensionally cultured endometrial cells were stained for integrin ${\alpha}1,\;{\alpha}4,\;{\beta}3$, and cyclooxygenase-l, -2 by immunohistochemistry, which all showed strong expression. The cultured epithelial cells showed the formation of microvilli, tight junctions and pinopodes by electron microscopy. Studies are currently under way utilizing this three dimensional culture model to ascertain the interaction between the embryo and human endometrial cells at the time of implantation, and it is thought that further studies into a new culture environment which would allow longer periods of culture will be necessary.

• 한국식품과학회지
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• 제31권5호
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• pp.1268-1274
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• 1999

본 연구에서 행하여진 온도$(35,\;55^{\circ}C)$ 및 압력(100, 300bar) 범위에서 초임계 추출된 솔 추출물의 수율은 300bar, $35^{\circ}C$와 300 bar, $55^{\circ}C$의 경우 각각 5.0%와 5.3%이었으며 100 bar, $35^{\circ}C$와 100 bar, $55^{\circ}C$에서 각각 3.7% 및 2.8%이었다. 300 bar에서는 wax류 등의 추출로 인하여 얻어진 추출물들은 상온에서 고체 상태이며 노란 색이었고, 100 bar에서 얻어진 추출물은 모두 액체 상태이고 무색에 가까웠다. 관능검사 결과 100 bar, $35^{\circ}C$에서의 초임계 추출물이 SDE 추출물보다 1% 유의수준에서 우수함이 판명되었다. 그러나 100 bar, $35^{\circ}C$에서의 추출물과 300 bar, $55^{\circ}C$ 추출물 간에는 5% 유의수준에서 차이가 없었다. 가장 많이 함유되어 있는 휘발성 향기 성분은 limonene으로써 $32.6{\sim}43.4%$를 차지하였고 ${\beta}-pinene(10.8{\sim}18.5%),\;{\beta}-myrcene(11.5{\sim}17.3%),\;{\alpha}-pinene(5.3{\sim}11.7%)$의 순이었다. 한편 향의 품질 특성에 결정적인 역할을 하는 것으로 알려진 함산소 terpene의 경우 100 bar, $35^{\circ}C$의 경우가 가장 많아서 3.6%를 차지하였으며 300 bar, $35^{\circ}C$의 경우에도 3.4%로서 300 bar, $55^{\circ}C$의 2.5%, 100 bar. $55^{\circ}C$의 1.8%보다 많았다. SDE 추출물의 경우는 6.7%를 차지하여 초임계 추출물보다도 훨씬 많은 양을 나타내었다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1163-1168
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• 2016

Cholangiocarcinoma (CCA) is the bile duct cancer which constitutes one of the important public health problems in Thailand with high mortality rate, especially in the Opisthorchis viverrini (a parasite risk factor for CCA) endemic area of the northeastern region of the country. This study aimed to identify potential biomarkers from the plasma peptidome by CCA patients. Peptides were isolated using 10 kDa cut-off filter column and the flow-through was then used as a peptidome for LC-MS/MS analysis. A total of 209 peptides were obtained. Among these, 15 peptides were concerned with signaling pathways and 12 related to metabolic, regulatory, and biosynthesis of secondary metabolite pathways. Five exclusive peptides were identified as potential biomarkers, i.e. ETS domain-containing transcription factor ERF (P50548), KIAA0220 (Q92617), phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 1 (P42338), LP2209 (Q6XYC0), and casein kinase II subunit alpha (P19784). Three of these biomarkers are signaling related molecules. A combination of these biomarkers for CCA diagnosis is proposed.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6319-6325
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• 2012

We previously found cytotoxic effects of tomato leaf extract (TLE) on the MCF-7 breast cancer cell line. The aim of this study was to ascertain the molecular mechanisms associated with the usage of TLE as an anticancer agent by microarray analysis using mRNA from MCF-7 breast cancer cells after treatment with TLE for 1 hr and 48 hrs. Approximately 991 genes out of the 30,000 genes in the human genome were significantly (p<0.05) changed after the treatment. Within this gene set, 88 were significantly changed between the TLE treated cells and the untreated MCF-7 cells (control cells) with a cut-off fold change >2.00. In order to focus on genes that were involved in cancer cell growth, only twenty-nine genes were selected, either down-regulated or up-regulated after treatment with TLE. Microarray assay results were confirmed by analyzing 10 of the most up and down regulated genes related to cancer cells progression using real-time PCR. Treatment with TLE induced significant up-regulation in the expression of the CRYAB, PIM1, BTG1, CYR61, HIF1-${\alpha}$ and CEBP-${\beta}$ genes after 1 hr and 48 hrs, whereas the TXNIP and THBS1 genes were up-regulated after 1 hr of treatment but down-regulated after 48 hrs. In addition both the HMG1L1 and HIST2H3D genes were down-regulated after 1 hr and 48 hrs of treatment. These results demonstrate the potent activity of TLE as an anticancer agent.

• Corrosion Science and Technology
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• 제8권4호
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• pp.143-147
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• 2009

In biomedical implants and dental fields, titanium has been widely utilized for excellent corrosion resistance and biocompatibility. However, Ti and its alloys are nonbioactive after being implanted in bone. In this study, for the purpose of improvement in biocompatibility the anodic $TiO_2$ layer on Ti-xNb alloys were fabricated by electrochemical method in phosphate solution, and the effect of Nb content on the pore size, the morphology and crystallinity of Ti oxide layer formed by the anodic oxidation method was investigated. The Ti containing Nb up to 3 wt%, 20 wt% and 40 wt% were melted by using a vacuum furnace. The sample were cut, polished, and homogenized for 24 hr at $1050^{\circ}C$ for surface roughness test and anodizing. Titanium anodic layer was formed on the specimen surface in an electrolytic solution of 1 M phosphoric acid at constant current densities ($30mA/cm^2$) by anodizing method. Microstructural morphology, crystallinity, composition, and surface roughness of oxide layer were observed by FE-SEM, XRD, EDS, and roughness tester, respectively. The structure of alloy was changed from $\alpha$-phase to $\beta$-phase with increase of Nb content. From XRD results, the structure of $TiO_2$ formed on the Ti-xNb surface was anatase, and no peaks of $Nb_2O_5$ or other Nb oxide were detected suggesting that Nb atoms are dispersed in $TiO_2$-based solid solution. Surface roughness test and SEM results, pore size formed on surface and surface roughness decreased as Nb content increased. From the line analysis results, intensity of Ti peak was high in the center of pore, whereas, intensity of O peak was high in the outside of pore center.