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Growth Inhibition of Plant Pathogenic Fungi by a Chitinase of Coprinellus congregatus (먹물버섯의 키틴질 분해효소에 의한 식물병원균류의 생장억제)

  • Kang, Yuri;Choi, Hyoung T.
    • Korean Journal of Microbiology
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    • v.48 no.4
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    • pp.325-327
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    • 2012
  • Experiments for growth inhibition of two different plant pathogenic fungal species, Alternaria alternata (KCTC26781) and Fusarium graminearum ZO3639 (type culture) and ASR1R1 (isolate from rice), by a chitinase (Chi2) expressed in the autolysing tissue of Coprinellus congregatus were carried out. In liquid media, Chi2 ($50{\mu}g/ml$) inhibited more than 90% of germination of A. alternata spore, and the growth of each fungal strain was totally inhibited by the addition of Chi2 at the concentrations of $70{\mu}g/ml$. When $6{\mu}g$ of Chi2 was added twice a day at the hyphal tip zone, both strains of F. graminearum showed growth inhibition as well as decreased hyphal branching.