• Title/Summary/Keyword: %24Fe_4N%24

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Rhizome Rot incidence of Platycodon grangiflorus as Influenced by the Soil Chemical Properties and Microbial Flora (토양 화학성과 미생물상이 도라지 근경부패병에 미치는 영향)

  • Lee, Young-Han;Kim, Jong-Gyun;Park, Sang-Ryeol;Choi, Yong-Jo;Cho, Soo-Jeong;Yun, Han-Dae
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.62-66
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    • 2000
  • This study was conducted to determine the soil chemical properties and microbial flora of platycodon and their effect on rhizome rot incidence. Sampling sites were Keochang 4, Kimhae 7, Haman 6, Chinju 6 and Koseong 3 fields in Kyongnam province and Hongcheong 6 fields in Kangwon province. The root disease incidence rate was in the order of Haman 56.8%, Hongcheon 52.5%, Kimhae 36.7%, Koechang 35.3%, Chinju 32.3%, Koseong 30.0%. The yield at the Chinju 36.17 Mg/ha was higher than that of Koseong 25.00 Mg/ha, Kimhae 13.57 Mg/ha, Koechang 11.75 Mg/ha, Haman 9.50 Mg/ha, Hongcheon 5.24 Mg/ha. The soil K, $NH_4-N$ content and EC value of injury root were higher than those of normal root. The root CaO content was positively correlated with yield $Y=20505X-367.9(R^2=0.129^{\ast})$. The Fe and Zn content of normal root were higher than those of injury root, while the T-N, $P_2O_5$ and $K_2O$ content of normal root were lower than those of injury root. The population of bacteria, actinomycetes and bacteria/fungi ratio increased in soil of normal root, but that of fungi decreased.

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A Study on Deep Levels in Rapid Thermal Annealed PICTS Semi-Insulating InP(100) by PICTS (PICTS 방법에 의한 급속열처리시킨 반절연성 InP(100)에서 깊은준위에 관한 연구)

  • 김종수;김인수;이철욱;이정열;배인호
    • Electrical & Electronic Materials
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    • v.10 no.8
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    • pp.800-806
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    • 1997
  • The behavior of de levels in rapid thermal annealed Fe-doped semi-insulating InP(100) was studied by photoinduced current transient spectrocopy(PICTS). In bulk InP, T2(Ec-0.24 eV), T3(Ec-0.30 eV) and T5(Ec-0.62 eV) traps were observed. After annealing the T2 trap was annihilated at 20$0^{\circ}C$ and recreated at 35$0^{\circ}C$. T3 trap was not affected below 40$0^{\circ}C$. With increasing temperature the concentration of T5 trap reduced and it was annihilated at 30$0^{\circ}C$. However the T1(Ec-0.16 eV) and T4(Ec-0.42 eV) traps were began to appear at 40$0^{\circ}C$and these concentrations were increased with annealing temperature. The T1 and T4 traps seem to be related to the isolated phosphorus vacancy( $V_{p}$) and $V_{p}$-indium antisite( $V_{p}$- $P_{in}$ ) or $V_{p}$-indium interstitial( $V_{p}$-I $n_{I}$) respectiely.respectiely.

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Purification and Characterization of an Alkali-Thermostable Lipase from Thermophilic Anoxybacillus flavithermus HBB 134

  • Bakir, Zehra Burcu;Metin, Kubilay
    • Journal of Microbiology and Biotechnology
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    • v.26 no.6
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    • pp.1087-1097
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    • 2016
  • An intracellular lipase from Anoxybacillus flavithermus HBB 134 was purified to 7.4-fold. The molecular mass of the enzyme was found to be about 64 kDa. The maximum activity of the enzyme was at pH 9.0 and 50℃. The enzyme was stable between pH 6.0 and 11.0 at 25℃, 40℃, and 50℃ for 24 h. The Km and Vmax of the enzyme for pNPL substrate were determined as 0.084 mM and 500 U/mg, respectively. Glycerol, sorbitol, and mannitol enhanced the enzyme thermostability. The enzyme was found to be highly stable against acetone, ethyl acetate, and diethyl ether. The presence of PMSF, NBS, DTT and β-mercaptoethanol inhibited the enzyme activity. Hg2+, Fe3+, Pb2+, Al3+, and Zn2+ strongly inhibited the enzyme whereas Li+, Na+, K+, and NH4+ slightly activated it. At least 60% of the enzyme activity and stability were retained against sodium deoxycholate, sodium taurocholate, n-octyl-β-D-glucopyranoside, and CHAPS. The presence of 1% Triton X-100 caused about 34% increase in the enzyme activity. The enzyme is thought to be a true lipase since it has preferred the long-chain triacylglycerols. The lipase of HBB 134 cleaved triolein at the 1- or 3-position.

Studies on the Growth and nutritional Behaviors of Seedlings Sand Cultured at Different pH Levels (pH 수준별(水準別) 사경묘목(砂耕苗木)의 생장(生長)과 영양동태(營養動態)에 관(關)한 연구(硏究))

  • Son, Won Ha
    • Journal of Korean Society of Forest Science
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    • v.11 no.1
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    • pp.1-24
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    • 1970
  • This experiment was carried out to elucidate the behavior of nutrition absorption and the acid tolerance, on varying soil pH levels, of Pinus thunbergii, Pinus densiflora, Pinus rigida, PInus koraiensis, Larix leptolepis and Abies holophylla which are 6 month old seedlings through examinations of the absorption behavior, the acid and alkali tolerance of N,P,K, Ca and Fe in the sand culture media with artificially controlled pH. 1. The increase rate of dry matter conditioned by the behavior of nutrition absorption in a strong acidic condition (pH3) was found in the following decreasing order of Pinus rigida, Pinus thunbergii. This order, therefore, can also be said to apply to the case of acid tolerance. 2. The increase rate of dry matter in a strong alkaline condition (pH9) was found in the following decreasing order of Pinus thunbergii, Pinus rigida, Pinus densiflora, Pinus koraiensis, Abies hoplohylla, Larix leptolepis. This order, therefore can be said to apply to the case of alkali tolerance. 3. Considering the absorption behavior of K and Ca, it is possible to decide the tolerance of acid from the absorption behavior and it can be said that if the absorption quantity is K>Ca the acid tolerance is strong and vice versa. 4. Considering the ratio of K:Fe and Ca:Fe in this experiment, a strong acid tolerance shows a higher ratio of K/Fe and vice versa. And also a strong acid tolerance shows a lower ratio of Ca/Fe and vice versa. It is therefore possible to decide the acid tolerance from the absorption behavior of K, Ca and Fe. 5. Of the tree species subjected to this experiment, Pinus thunbergii was the strongest in both acid and alkali tolerance. 6. In spite of that in all species the dry weight in the root is smailer than that in the top, the quantity of Fe contained in the root was larger than that in the top.

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Physicochemical Characteristics of Various Ginseng Seeds (품종 별 인삼 씨의 이화학적 성분 비교)

  • Kim, Hee Jung;Yoo, Kyung Mi;Lee, Seul;Kim, Kyung-Tack;Hwang, In Kyeong
    • Korean Journal of Food Science and Technology
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    • v.45 no.3
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    • pp.274-278
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    • 2013
  • The aim of our study was to investigate the chemical composition of the Asian ginseng seed (Panax ginseng C.A. Meyer) and the American ginseng seed (Panax quinquifolium L.) grown in Korea (3 years, KGS3; 4 years, KGS4), China (4 years, CGS4), and USA (4 years, AGS4). AGS had the heaviest 100-seed weight ($4.21{\pm}0.31g$). The approximate compositions of the ginseng seeds were 13.66-17.00% crude protein, 2.21-8.65% crude ash, 19.06-24.06% crude lipid, and 43.21-47.49% crude fiber. The mineral contents of the ginseng seeds were greater in order of K>P>Ca>Mg>Fe>Na>Zn >Cu. The unsaturated fatty acid content was 96.71-96.94%, and the major fatty acids oleic acid and linoleic acid were present. Total sugar content was 15.00-26.17 mg glucose/g. The acidic polysaccharide content was 0.56-0.80 mg ${\beta}$-Dgalacturonic acid/g. These results showed the differences in the physicochemical characteristics of ginseng seeds with respect to cultivation location, cultivation year, and species.

Geologic Report on the Goobong Limestone Mine (구봉석회석광산의 지질조사보고(地質調査報告))

  • Lee, Dai Sung
    • Economic and Environmental Geology
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    • v.3 no.1
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    • pp.17-24
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    • 1970
  • The purpose of this report is to prepare a data for the economic evaluation on the Goobong Limestone Mine which is located at the south-eastern corner of the Yongchun Quadrangle scaled in 1:50,000. The accessibility from the mine to railroad was considered in two ways. One is to Dodam Station on Central Railway Line and the other is to reach Songjung-ni village which is near Sangyong Station on Hamback Railway Line. The distance of the former way is 26.7km and the later is 24.2km. Geologically the mine is situated near the base of the Greast Limestone Series which strikes generally $N25^{\circ}{\sim}30^{\circ}E$. The series comprises six different formations from older to younger; Pungchon Limestone Formation and Whajol Formation of Cambrian age, and Dongjum Quartzite Formation, Dumudong Formation, Maggol Limestone Formation and Goseong Formation of lower to middle Ordovician age. 82 samples; 48 from Pungchon Limestone Formation, 11 from Dumudong Formation, 15 from Maggol Limestone Formation and 8 from Goseong Formation, were taken from the series in the crossed direction to the general trend of the series as shown in geological map. They were chemically analyzed on the components of CaO, MgO, $SiO_2$, $R_2O_3(Al_2O_3+Fe_2O_3)$ and ignition loss as shown in table 2, table 3, table 4, and table 5. As seen from the tables, among the formations of the series, middle to upper parts of the Pungchon Limestone Formation and middle and upper parts of the Dumudong Formation have chemical composition as available source for the raw material of cement industry, not only that but also the part of the Pungchon Formation was highly evaluated as source for the flux of iron smelting and the raw material of carbide manufacturing because of its high purity of calcium carbonate.

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Elemental Composition of the Soils using LIBS Laser Induced Breakdown Spectroscopy

  • Muhammad Aslam Khoso;Seher Saleem;Altaf H. Nizamani;Hussain Saleem;Abdul Majid Soomro;Waseem Ahmed Bhutto;Saifullah Jamali;Nek Muhammad Shaikh
    • International Journal of Computer Science & Network Security
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    • v.24 no.6
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    • pp.200-206
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    • 2024
  • Laser induced breakdown spectroscopy (LIBS) technique has been used for the elemental composition of the soils. In this technique, a high energy laser pulse is focused on a sample to produce plasma. From the spectroscopic analysis of such plasma plume, we have determined the different elements present in the soil. This technique is effective and rapid for the qualitative and quantitative analysis of all type of samples. In this work a Q-switched Nd: YAG laser operating with its fundamental mode (1064 nm laser wavelength), 5 nanosecond pulse width, and 10 Hz repetition rate was focused on soil samples using 10 cm quartz lens. The emission spectra of soil consist of Iron (Fe), Calcium (Ca), Titanium (Ti), Silicon (Si), Aluminum (Al), Magnesium (Mg), Manganese (Mn), Potassium (K), Nickel (Ni), Chromium (Cr), Copper (Cu), Mercury (Hg), Barium (Ba), Vanadium (V), Lead (Pb), Nitrogen (N), Scandium (Sc), Hydrogen (H), Strontium (Sr), and Lithium (Li) with different finger-prints of the transition lines. The maximum intensity of the transition lines was observed close to the surface of the sample and it was decreased along the axial direction of the plasma expansion due to the thermalization and the recombination process. We have also determined the plasma parameters such as electron temperature and the electron number density of the plasma using Boltzmann's plot method as well as the Stark broadening of the transition lines respectively. The electron temperature is estimated at 14611 °K, whereas the electron number density i.e. 4.1 × 1016 cm-3 lies close to the surface.

Ferroelectric and Magnetic Properties of Dy and Co Co-Doped $BiFeO_3 $ Ceramics

  • Yu, Yeong-Jun;Park, Jeong-Su;Lee, Ju-Yeol;Gang, Ji-Hun;Lee, Gwang-Hun;Lee, Bo-Hwa;Kim, Gi-Won;Lee, Yeong-Baek
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.260-260
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    • 2013
  • Multiferroic materials have attracted much attention due to their fascinating fundamental physical properties and technological applications in magnetic/ferroelectric data-storage systems, quantum electromagnets, spintronics, and sensor devices. Among single-phase multiferroic materials, $BiFeO_3 $ is a typical multiferroic material with a room temperature magnetoelectric coupling in view of high magnetic-and ferroelectric-ordering temperatures (Neel temperature $T_N$~647 K and Curie temperature $T_C$~1,103 K). Rare-earth ion substitution at the Bi sties is very interesting, which induces suppressed volatility of Bi ion and improved ferroelectric properties. At the same time, Fe-site substitution with magnetic ions is also attracting, and the enhanced ferromagnetism was reported. In this study, $Bi_{1-x}Dy_xFe_{0.95}Co_{0.05}O_3$ (x=0, 0.05 and 0.1) bulk ceramic compounds were prepared by solid-state reaction and rapid sintering. High-purity $Bi_2O_3$, $Dy_2O_3$, $Fe_2O_3$ and $Co_3O_4$ powders with the stoichiometric proportions were mixed, and calcined at $500^{\circ}C$ or 24 h to produce $Bi_{1-x}Dy_xFe_{0.95}Co_{0.05}O_3$. The samples were immediately put into an oven, which was heated up to $800^{\circ}C$ nd sintered in air for 30 min. The crystalline structure of samples was investigated at room temperature by using a Rigaku Miniflex powder diffractometer. The field-dependent magnetization measurements were performed with a vibrating-sample magnetometer. The electric polarization was measured at room temperature by using a standard ferroelectric tester (RT66B, Radiant Technologies).

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황토의 인흘착 성능평가

  • 허영오;손지호
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 1997.10a
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    • pp.65-66
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    • 1997
  • 우리나라의 대부분 하천과 인공댐의 경우 조류발생에 기여하는 영양염은 질소, 인 그리고 규소 등인데 특히 인이 생산제한 인자로 작용하고 있다. 따라서 인을 적절히 제거할 경우에는 조류 발생에 의한 수질의 악화와 수이용의 저해요인을 배제할 수 있어 이에 대한 연구가 많이 진행되어 왔다. 지금까지와 연구는 인의 화학적 응집 침전법, 생물학적 처리법에 대하여 수행되어 왔으나 설비자금, 운영비, 운전기술, 슬러지 생성 그리고 제거효율 등에서 만족스런 결과가 도출되지 못하여 현장 적용을 하지 못하고 있는 실정이다. 이러한 현실을 감안하여 우리의 자연 환경에서 쉽게 자할 수 있는 황토를 모재로하여 Al3-과 Fe3-, Ca2-을 적절히 배합하여, 주로 인을 선택적으로 제거하 고자 개발된 황토의 흡착능력 그리고 흡착 메카니즘에 대한 기본적인 연구를 하고자 (1) 등온흡착실험을 통하여 흡착용량을 평가하고 (2) 흡착제거속도를 평 가하였고 (3)파과시간 및 흡착특성을 파악하기 위해 column 흡착실험을 하였다 또한 (4) 황토내의 Al3-, Fe3-과 Ca2- 등이 인의 화학적 흡착에 기여하는 정도를 파악하여 흡착메카니즘을 규명하고자 하였다. 먼저 흡착용량실험을 위하여 PO3-4-P 농도 3ppm의 용액 200mf에 황토 0.2g, 0.5g, 1.0g, 2.0g을 각각 투여한 후 충분한 흡착평형이 일어나게 24시간 동 안 130rpm으로 $25^{\circ}C$ 등온반웅조에서 저어주어 흡착평형에 도달하면 상등액을 GF/C Filter로 여과한 후, 여액에 대해 PO3-4-P의 농도를 Ascrobic he건법으로 측정한 결파, Freundlich 등온흡착식에 의하면 K값은 17.34와 16.28이었으며 1/n 값은 1.32와 1.42로 인흡착 성능이 뛰어난 것으로 평가되었다. 둘째, 흡착속도 실험은 PO3-4-P 농도 1.5ppm의 용액 2f에 259의 황토를 투여하고 충분한 혼합이 일어날 수 있도록 170rpm으로 교반하면서 시간별 용액 의 농도 변화를 측정한 결과, 0.45mg/g/m교의 속도로 15분만에 94.3%의 인 제거 효율을 보였다. 셋째, 직경 12mm의 glass column에 황토를 209 채우고 1.5ppm의 PO34P 용액을 2.Sne11in의 유량으로 통수 시킨 후, 시간에 따른 농도 변화를 측정한 결과, 원수 농도의 50%에 해당하는 파과점까지 약 70시간 만에 도달하였다. 넷째, Al3-, Fe3-과 Ca2- 등이 화학적 흡착에 기여하는 정도를 파악하기 위 하여 황토 2g에 대하여 Hieltijes and Lijklema 방법에 의해 Adsorbed-p, Nonapatite inorganic-P(NAI-P), Apatite-p, Organic-P로 구분하여 분석하고, 총인(Total Phosphorus)을 Standard Methods에 따라 Persulfate digestion후 0.45 m membrane 여지 여과하여 여액에 대해 PO3-4-P의 농도를 Ascorbic Acid 법으로 측정한 결과, NAI-P가 가장 큰 비율을 차지하였고, 부원료로 첨가된 금속 양이온 중 Fe3-이온이 흡착에 기여하는 정도가 가장 큰 것으로 평가되었다.

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The Biochemical Characterization of D-Hydroxyisovalerate Dehydrogenase, a Key Enzyme in the Biosynthesis of Enniatins

  • Lee, Chan; Zocher, Rainer
    • BMB Reports
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    • v.29 no.6
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    • pp.493-499
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    • 1996
  • The biochemical properties of purified D-hydruxyisovalerate dehydrogenase from Fusarium sambucinum was elucidated. D-Hydroxyisovalerate dehydrogenase produced solely D-hydroxyisovalerate from 2-ketoisovalerate. The isoelectric point of the purified enzyme was 7.0. The enzyme was highly specific with 2-ketoisovalerate ($K_{m}=0.188$ mM, $V_{max}=8.814$ mmol/min mg) and 2-keto-3-methyl-n-valerate ($K_{m}=0.4$ mM, $V_{max}=1.851$ mmol/min mg) for the reductive reaction. This was also seen by comparing D-hydroxyisovalerate ($K_{m}=1.667$ mM, $V_{max}=0.407$ mmol/min mg) and D-hydroxy-3-methyl-n-valerate ($K_{m}=6.7$ mM, $V_{max}=0.648$ mmol/min mg) for the oxidative reaction. Thiol blocking reagents, such as iodoacetamide, N-ethylmaleimide and p-chloromecuribenzoate inhibited about 80% of enzyme activity at 0.02 mM, 50 mM and 50 mM, respectively. The enzyme activity was also inhibited by the addition of 0.1 mM of various metal ions, such as $Fe^{2+}$ (67%), $Cu^{2+}$ (88%), $Zn^{2+}$ t (76%) and $Mg^{2+}$ (9%). The enzyme was stable over three months in 50 mM potassium phosphate buffer (pH 5~7) at $-80^{\circ}C$. However the purified enzyme lost 30% of its activity in the same buffer after 24 h at $4^{\circ}C$. The studies about thermal inactivation of D-hydroxyisovalerate dehydrogenase exhibit 209.2 kJ/M of activation enthalpy and 0.35 kJ/mol K of activation entropy.

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