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Physicochemical Properties of Mucilage from Domestic Molokhia (Corchorus olitorius) (몰로키아(Corchorus olitorius) 잎에서 추출한 mucilage의 이화학적 특성)

  • Jung, Chang-Hwa;Choi, In-Wook;Kim, Heung-Man;Seog, Ho-Moon
    • Korean Journal of Food Science and Technology
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    • v.34 no.5
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    • pp.757-761
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    • 2002
  • Proximate composition, phenolic compounds, and amino acid composition of molokhia (Corchorus olitorius) and molecular weight distribution of its mucilage were determined. Concentrations of dietary fibers and protein were 37.4 and 24.4% of the total dry weight, respectively. Mucilage was obtained by extracting dry molokhia leaves with hot water, followed by the addition of 55% ethanol. Although about 50% of the mucilage was lost during the extration, it was still rich in polyphenol compounds. In mucilage, proteins were composed of glutamic acid, glycine, alanin, and aspartic acid, and molecular weight of most polysaccarides ranged from $40,000{\sim}500,000$ daltons based on gel filtration chromatography.

Effects of Supplementation of Probiotics on the Performance, Nutrient Digestibility and Faecal Microflora in Growing-finishing Pigs

  • Giang, Hoang Huong;Viet, Tran Quoc;Ogle, Brian;Lindberg, Jan Erik
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.5
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    • pp.655-661
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    • 2011
  • Two experiments were conducted to investigate the effect of dietary supplementation of Bacillus, Saccharomyces and lactic acid bacteria (LAB) on performance and nutrient digestibility in grower and finisher pigs. In Exp. 1, 80 pigs (32 females and 48 males), $28.7{\pm}0.9\;kg$ body weight (BW), were randomly divided into 4 treatment groups balanced for sex and weight (5 pigs per pen, 4 pens per treatment). They were fed one of four diets: a basal grower (20-50 kg BW) and finisher (>50 kg BW) diet without any addition of probiotic or antibiotic (diet C), the basal diet supplemented with Bacillus subtilis H4 (diet B), diet B supplemented with Saccharomyces boulardi Sb (diet BS) and diet BS supplemented with a LAB complex (diet BSL). The LAB complex consisted of Enterococcus faecium 6H2, Lactobacillus acidophilus C3, Pediococcus pentosaceus D7, and Lactobacillus fermentum NC1. In Exp. 2, 16 male pigs, $29.2{\pm}0.8\;kg$ BW, were kept in individual pens and divided into 4 groups (4 pigs in each group). All 4 groups were given exactly the same growing-period diets (diet C, B, BS and BSL) as in Exp 1. The total faeces and urine were collected during 5 days (day 20-24) to determine nitrogen retention and total tract digestibility. In the growing period, average daily feed intake (ADFI), average daily gain (ADG) and feed conversion ratio (FCR) were not affected by diet B and BS (p>0.05), but ADG increased (+5.9%) (p<0.05) and FCR improved (+5.9%) (p<0.05) on diet BSL compared with the control, although ADFI was not different (p>0.05). Digestibility of crude protein and organic matter was higher (p<0.05) in diet BSL and digestibility of crude fibre was higher (p<0.05) in diet BS and BSL than in diet C. Nitrogen retention was not affected by diet (p>0.05). The faecal LAB counts were increased in grower pigs fed diet BSL (p<0.05) and faecal E. coli counts were decreased in pigs fed diets BS and BSL (p<0.05). In the finishing period, no effects of diet were found in ADFI, ADG, FCR, nutrient digestibility, and nitrogen retention (p>0.05). Faecal LAB and E. coli counts in the finisher pigs were not affected by diet (p>0.05). In conclusion, the current study demonstrates that a mixture of bacteria and yeast has the potential to be used as a probiotic dietary supplement in grower pigs.

Investigation of Antimicrobial Activity and Stability of Orixa japonica Thunb. Leaf Extract (상산나무 잎 추출물의 항균활성 및 안정성 조사)

  • Choe, Su-Bin;Kang, Sung-Tae
    • Korean Journal of Food Science and Technology
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    • v.46 no.1
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    • pp.39-43
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    • 2014
  • The antimicrobial activity of Orixa japonica Thunb. leaf extract towards 13 microorganism strains was evaluated. Both methanol (MEex) and 70% ethanol extracts showed antimicrobial activity towards Streptococcus mutans, Bacillus cereus, Staphylococcus aureus, and Pseudomonas aeruginosa. MEex showed a higher antimicrobial activity than the 70% ethanol extract. In addition, the dichloromethane fraction (DCMfr) of the MEex also had an antimicrobial effect against the microorganisms examined. The minimum inhibitory concentrations (MICs) towards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 49.22, 24.61, 49.22, and 49.22 mg/mL, respectively. In contrast, the MICs of the DCMfr tpwards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 3.31, 0.21, 1.7, and 1.7 mg/mL, respectively. The MEex antimicrobial activity was not affected by a 3 h exposures to pH in the range of 3-11 or by temperatures were maintained between $80^{\circ}C-100^{\circ}C$ for 6 h. However, the MEex antimicrobial activity decreased at a heat treatment of $121^{\circ}C$ 1 h.

Purification and Characterization of a Maltopentaose-producing Amylase from Bacillus megaterium KSM B-404. (Bacillus megaterium KSM B-404으로부터 생산되는 Maltopentaose생성 Amylase의 정제 및 특성)

  • 박제원;김병주;이재우;김영배
    • Microbiology and Biotechnology Letters
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    • v.30 no.4
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    • pp.352-358
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    • 2002
  • An amylase that hydrolyzes starch into maltopentaose as a main product was found in the culture supernatant of a strain of Bacillus megaterium KSM B-404 isolated from local soil. The enzyme was purified 129-fold by ammonium sulfate precipitation, DEAE-Toyopearl and Superdex 75 HR 10/30 column using a FPLC system. The molecular weight of the amylase was determined as about 68 kDa by using SDS-PAGE. Optimum pH and temperature of amylase were found to be $50^{\circ}C$ and pH 6.0~7.0, respectively. The enzyme was stable up to $60^{\circ}C$ by addition of $Ca^{2+}$ and its pH stability was in the range of 6.0~10.0. The activity of enzyme was inhibited by $Cu^{2+}$ $Hg^{2+}$ , and $Fe^{3+}$ and maintained by $Ca^{2+}$ and $Mg^{2+}$ . EDTA and pCMB also showed inhibitory effect to the enzyme. TLC and HPLC analysis of the products of the enzyme reaction showed the presence of maltopentaose(52%), maltotriose (25%), maltose (11%), glucose, and maltotetraose in the starch hydrolysates.

Evaluation of malnutrition status and related risk factors in geriatric outpatient clinic

  • Cin, Pelin;Tanriover, Ozlem;Yavuzer, Hakan;Erdincler, Deniz Suna
    • Nutrition Research and Practice
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    • v.15 no.4
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    • pp.504-515
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    • 2021
  • BACKGROUND/OBJECTIVES: Malnutrition risk and malnutrition among the elderly is a public health concern. In combating this health-related problem, it is critically important to evaluate the risk factors in a multidimensional way and to apply appropriate nutrition intervention based on the results. SUBJECTS/METHODS: A cross-sectional study was conducted on 215 elderly patients (32.6% male, 67.4% female) in a geriatric outpatient clinic of a hospital in Turkey. Nutritional questionnaires that incorporated the 24-h recall method were applied to determine general characteristics of patients, their health status, nutritional habits, and daily energy and nutrient intakes. Mini Nutritional Assessment was used to determine nutritional status. Relevant anthropometric measurements were obtained. RESULTS: The subjects' mean age was 76.1 ± 7.0 years, and the prevalence of malnutrition (n = 7) and risk of malnutrition (n = 53) among the 215 subjects was 3.2% and 24.7%, respectively. Patients with malnutrition or risk of malnutrition were found to be single, have a depression diagnosis, in an older age group, have less appetite, more tooth loss, have more frequent swallowing/chewing difficulty, and have more frequent meal skipping. In addition, mean daily energy, carbohydrate, fat, fiber, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin C, folates, potassium, magnesium, phosphorus, iron intake, and water consumption were found to be statistically significantly low in subjects with malnutrition or risk of malnutrition. After performing regression analysis to determine confounding factors, malnutrition risk was significantly associated with marital status, loss of teeth, appetite status, and depression. CONCLUSIONS: Routine nutritional screening and assessment of the elderly should be performed. If nutritional deficiencies cannot be diagnosed early and treated, self-sufficiency in the elderly may deteriorate, resulting in increased institutionalization.

Trends of Antifungal Agent Susceptibility of Candida Strains Isolated from Blood Cultures in 2009~2018 (2009~2018년 혈액배양으로부터 분리된 Candida 균종의 항진균제 감수성의 경향)

  • Hwang, Yu-Yean;Kang, On-Kyun;Park, Chang-Eun;Lee, Moo-Sik;Kim, Young-Kwon;Huh, Hee-Jae;Lee, Nam-Yong
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.2
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    • pp.133-141
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    • 2022
  • Candida is one of the most common causes of bloodstream infections and a leading cause of morbidity and mortality among hospitalized patients. The purpose of this study was to provide important information for formulating empirical treatment plans for candidemia by investigating the antifungal resistance rate of Candida. Among the Candida strains (973 cases) isolated from blood culture tests at the S hospital in 2009~2018, 4.7% (N=44) comprising the Candida spp. (932 strains) showed resistance to fluconazole. The resistant strains included C. albicans, C. parapsilosis, C. tropicalis, and C. glabrata. In addition Candida spp. (947 strains) showed resistance to amphotericin B (N=6, 0.6%), flucytosine (N=23, 2.4%) and voriconazole (N=24, 3.1%). C. albicans was resistant to fluconazole (N=23, 6.9%) and voriconazole (N=21, 6.0%), The statistical analysis showed that C. albicans and non-albicans Candida species were resistant to fluconazole (P=0.039) and voriconazole (P<0.001). A monitoring system to understand the rate of candidiasis infections in a hospital setting is required. It is also important to make the right choice of the antifungal agent based on drug susceptibility patterns. Therefore, an infection surveillance policy that tracks Candida resistance through regular antifungal susceptibility tests is necessary.

Karyotype Analysis of Lilium cernum Komrov by Means of C-banding Method (Giemsa 분염법에 의한 솔나리의 핵형 분석)

  • 손진호
    • Journal of Plant Biology
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    • v.21 no.1_4
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    • pp.29-32
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    • 1978
  • The karyotype of Lilium cernum has been analysed by means of C-banding technique. Most of clones observed were 2n=24 chromosomes which consist of two pairs of submetacentric and ten pairs of subtelocentric chromosomes, among which two pairs of chromosomes(B and E) showed secondary constriction in the short arm. In addition to these chromosomes a small supernumerary telocentric chromosome was seen in the eight clones. Sixtyeight bands were observed in the twentytwo chromosomes of complement and one band in the supernumerary chromosome. A pair of chromosome (L) did not show any band. The bnads on the chromosome. A pair of chromosome (L) did not show any band. The bands on the chromosomes were distributed in the centromere, secondary constriction and intercalary regions of arms. Of the twelve pairs of chromosomes ten pairs showed symmetric banding patterns in each, but two pairs (I and K) showed asymmetric banding patterns.

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The effect of stable oxide dispersoid on the high temperature oxidation behavior of Ni-Cr alloys (Ni-Cr 합금의 고온산화특성에 미치는 안정한 산화물 분산체 효과)

  • 유재민;박상환;강성군
    • Journal of the Korean institute of surface engineering
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    • v.24 no.4
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    • pp.222-229
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    • 1991
  • The oxidation behavior of Ni-6wt.% Cr alloys with various concentration of alumina dispersions(5vol.% 10vol.%) was determined at 1000 and 110$0^{\circ}C$ in oxygen of 1 atm. pressure. Some general observations were ascertained. These include: (a) the addition of alumina dispersoids significantly increased the oxidation resistance of Ni-6wt.% Cr alloy; (b) the rate of oxidation was distinctly decreased as a function of the volume percent of oxide dispersion; and (c) the presence of alumina dispersions promoted the formation of continous, protective Cr2O3 layer at 6 wt.% Cr.

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Studies on the Estimation of Growth Pattern of Meat in the Edible Parts of Broilers in Growing Stages (육용계에 있어서 성장단계에 따른 부위별 가식육의 증가양상 추정에 관한 연구)

  • 김재홍;한성욱;오봉국
    • Korean Journal of Poultry Science
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    • v.9 no.2
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    • pp.63-101
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    • 1982
  • A study was conducted to investigate the possibility of improving the effectiveness of the existing method(s) to estimate the edible meat weight in live broiler chicken. Four. breeds of chicken, white Cornish broiler chicks(parent stock), Single Comb White Leghorn(SCWL) egg strain chicks (parent stock), and two reciprocal cross breds of these parent stocks were employed in this study. A total of 480 birds, 60 male and 60 female chicks for each breed, were used. Ten male and 10 female chicks from each breed were randomly sacrificed at 0, 2, 4, 6, 8 and 10 weeks of ages in order to measure the body weight, edible meat weight of breast and 1eg(thigh and drum-stick), various components of body weight and various body shape measurements. In addition, chemical analyses of edible meat were carried out. Results obtained from this sturd were summarized as follows. 1. The average body weights of White Cornish broiler chicks and SCWL egg strain chicks were 1.82${\pm}$0.048kg(X${\pm}$S.E.) and 0.67${\pm}$0.029 kg. respectively, at 8 weeks of age. The feed to gain ratio for White Cornish and SCWL chicks were 2.24 and 3.28, respectively, for 0 to 8 weeks of age. The reciprocal cross bred chicks (White Cornish ${\times}$ SCWL) showed intermediate values in both parameters.

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Study on the Microwave Dielectric Properties and Dielectric Resonator Performance of the $Mg_4Ta_2O_9$ Ceramics with $TiO_2$ Addition ($TiO_2$ 첨가에 따른 $Mg_4Ta_2O_9$ 세라믹스의 마이크로파 유전특성과 유전체 공진기 거동에 관한 연구)

  • Choi, Eui-Sun;Ryu, Ki-Won;Lee, Young-Hie;Kim, Jae-Sik
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.56 no.4
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    • pp.756-760
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    • 2007
  • The $(1-x)Mg_4Ta_2O_9-xwt%TiO_2\;(x=5\sim20)$ microwave dielectric ceramics were prepared by solid-state reaction method and sintered at $1450^{\circ}C$. According to the X-ray diffraction data, the $Mg_4Ta_2O_9-xwt%TiO_2$ ceramics had main phase of $Mg_4Ta_2O_9$ and $MgTi_2O_5$ peaks were added by increasing of $TiO_2$ addition. Microwave dielectric properties of the $Mg_4Ta_2O_9-xwt%TiO_2$ ceramics were influenced by $MgTi_2O_5$ phase and properties of $TiO_2$. There was a little decrement of the quality factor from 116,800GHz of pure $Mg_4Ta_2O_9$ to 100,100GHz of 15wt% $TiO_2$ added one. But there was excellent improvement in temperature coefficient of the resonant frequency (TCRF) by addition of 15wt% $TiO_2$. The dielectric constant quality factor and TCRF of the $Mg_4Ta_2O_9-xwt%TiO_2$ ceramics sintered at $1450^{\circ}C$ were $13.08\sim16.41,\;45,000\sim165,410GHz,\;-24.82\sim+3.88ppm/^{\circ}C$, respectively, depending on the value of x. Simulated dielectric resonator (DR) with $Mg_4Ta_2O_9-15wt%TiO_2$ ceramics had the operating frequency of 11.97GHz and $S_{2,1}$ of -35.034dB.