• Asian Pacific Journal of Cancer Prevention
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• 제16권5호
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• pp.1781-1787
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• 2015

Background: miRNAs are relatively recently discovered cancer biomarkers which have important implications for cancer early diagnosis, treatment and estimation of prognosis. Here we focussed on expression of mir-196a-5p in gastric cancer tissues and cell lines so as to analyse its significance for clinicopathologic characteristics and generate enriched KEGG pathways clustered by target genes for exploring its potential roles as a biomarker in gastric cancer. Materials and Methods: The expression of mir-196a-5p in poorly, moderate and well differentiated gastric cancer cell lines compared with GES-1 was detected by RT-qPCR, and the expression of mir-196a-5p in gastric cancer tissues comparing with adjacent non cancer tissues of 58 cases were also assessed by RT-qPCR. Subsequently, an analysis of clinical significance of mir-196a-5p in gastric cancer and enriched KEGG pathways was executed based on the miRWalk prediction database combined with bioinformatics tools DAVID 6.7 and Mirfocus 3.0. Results: RT-qPCR showed that mir-196a-5p was up-regulated in 6 poorly and moderate differentiated gastric cancer cell lines SGC-7901, MKN-45, MKN-28, MGC-803, BGC-823, HGC-27 compared with GES-1, but down-regulated in the highly differentiated gastric cancer cell line AGS. Clinical data indicated mir-196a-5p to beup-regulated in gastric cancer tissues (47/58). Overexpression of mir-196a-5p was associated with more extensive degree of lymph node metastasis and clinical stage (P < 0.05; x2 test). Enriched KEGG pathway analyses of predicted and validated targets in miRWalk combined with DAVID 6.7 and Mirfocus 3.0 showed that the targeted genes regulated by mir-196a-5p were involved in malignancy associated biology. Conclusions: Overexpression of mir-196a-5p is associated with lymph node metastasis and clinical stage, and enriched KEGG pathway analyses showed that targeted genes regulated by mir-196a-5p may contribute to tumorgenesis, suggesting roles as an oncogenic miRNA biomarker in gastric cancer.

• 대한치과의사협회지
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• 제50권9호
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• pp.566-573
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• 2012

Purpose : This study evaluated the impact of the presence and aspect of mandibular third molars to the mandible angle fracture or condyle fractures in Korean. Materials and Methods : A retrospective study was designed for patients attending the division of Oral and Maxillofacial Surgery, Kang-dong sacred heart hospital for treatment of mandibular fracture from January 2006 to September 2010. The primary variable was the presence of mandibular third molar and the secondary variable was the aspects of third molar impaction. Mandibular third molars were classified by the impaction depth and the available space as Pell & Gregory system. Outcome variables were the presence of mandibular angle fracture or condyle fracture. Also the source of trauma, age, sex were studied. Hospital charts, radiographs were used for study. Statistic analysis was done with descriptive statistics, the X2-test, linear-by-linear association. P value under 0.05 was considered significant statistically. Results : The number of involved patients was 86. The ratio of male to female patients was about 9:1 for angle fracture and 7:3 for condyle fracture. The most common source of trauma was assault for angle fracture and fall down for condyle fracture. The presence of mandibular third molar increased frequency of angle fracture and decreased condyle fracture with larger impaction depth. But available space of mandibular third molar did not show high association with angle or condyle fractures. Conclusion: Preventive extraction of mandibular third molar is recommended for patients with high risk of angle fracture. Male patients at their third decade or martial artists, police officer could be the case. But it is not recommended for patients with low risk of angle fracture and high risk of condyle fracture relatively. Elder female patients without any symptom on their third molar could be the case.

• Asian-Australasian Journal of Animal Sciences
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• 제30권2호
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• pp.206-210
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• 2017

Objective: A study was conducted to test six threonine (Thr) levels (0.39%, 0.44%, 0.49%, 0.54%, 0.59%, and 0.64%) to estimate the optimal dietary Thr requirements for Longyan laying ducks from 17 to 45 wk of age. Methods: Nine hundred Longyan ducks aged 17 wk were assigned randomly to the six dietary treatments, where each treatment comprised six replicate pens with 25 ducks per pen. Results: Increasing the Thr level enhanced egg production, egg weight, egg mass, and the feed conversion ratio (FCR) (linearly or quadratically; p<0.05). The Haugh unit score, yolk color, albumen height, and the weight, percentage, thickness, and breaking strength of the eggshell did not response to increases in the Thr levels, but the albumen weight and its proportion increased significantly (p<0.05), whereas the yolk weight and its proportion decreased significantly as the Thr levels increased. Conclusion: According to a regression model, the optimal Thr requirement for egg production, egg mass, and FCR in Longyan ducks is 0.57%, while 0.58% is the optimal level for egg weight from 17 to 45 wk of age.

• 한국식품저장유통학회지
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• 제15권6호
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• pp.915-921
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• 2008

본 연구에서는 버섯 선호도와 섭취빈도 등을 알아보고 버섯가공식품에 대한 소비자의 인식 등을 조사하여 소비촉진을 위한 버섯가공기술 개발을 위한 기초자료를 얻고자 하였다. 총 조사 대상자는 1173명으로 성별로는 남자가 370명(31.5%), 여자가 803명(68.5%)으로 구성되었고, 조사대상자의 평균 연령은 20.0세였으며 조사대상자의 333명(28.5%)이 새송이 버섯을 가장 선호하는 것으로 조사되었다. 연령별에 따른 선호하는 버섯의 경우는 20$\sim$60대 이상의 전 연령층의 333명(28.5%)이 새송이 버섯을 가장 선호하였으며 그 다음으로 팽이 버섯 241명(20.6%), 표고버섯 223명(19.1%)의 순으로 나타났다. 선호하는 버섯에 대한 이유로 대상자의 380명(32.4%)이 좋은 조직감을 제일 우선순위 1위로 응답하였으며, 한 달에 섭취하는 버섯의 빈도는 여성이 한 달에 8.24회를 섭취하여 남성의 6.98회 보다 1.26회 더 자주 섭취하는 것으로 조사되었고, 버섯 구입 시 고려해야할 사항에 대하여 신선도.위생 상태를 1위로 응답하였다. 버섯가공식품을 구입해본 경험이 있는지에 대하여 조사한 결과, 조사대상자의 55.5%가 먹기 위하여 구입해본 적 있다고 답하였으며 그 중 구입해 본 버섯가공식품으로는 스프, 죽 > 건조 분말 > 건강기능식품 등 이었다. 버섯가공식품 구입 시 고려할 사항으로 맛의 향상을 1위로 응답하였다. 새로운 버섯가공식품을 개발할 경우, 연령별 선호하는 제품을 조사한 결과 20대$\sim$60대 이상의 모든 연령층에서 스프와 죽을 가장 선호하는 것을 알 수 있었으며, 특히 $20{\sim}29$세는 햄버거 패티, $30{\sim}59$세는 어묵 그리고 60세 이상은 버섯 병조림(34.3%)을 가장 개발되기를 희망하는 버섯가공식품으로 응답하였다. 본 조사결과를 토대로 버섯의 소비를 촉진시키기 위하여 버섯을 이용한 다양한 가공식품 개발이 필요하다고 사료된다.

• 한국보건간호학회지
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• 제7권1호
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• pp.31-48
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• 1993

Nowaday, there have a lot of changes in the demands of the aged persons. Their problems also came to the fore with diverse forms under the influences of industrialization, urbanization and nuclear family. To make the matter worse, the aged population is mounting rapidly. Also, such structure as nuclear family is widely disseminating uncomfortable to the aged. People is mainly being guided by self interest above everything else. Indeed, they had, all together, bad effects on our traditional value system regarding 'respect for the aged and devotion to patients'. It seems unfortunately obvious that the family responsibility is gradually weakening to support the old who is a dependent family. The result is that the aged must have suffered all sorts of hardships in lightenning psychological, physical and economical difficulties. First, to grasp the situations and conditions supporting for single aged persons from each view of psychological, emotional, family-relational, healthy, social and economical standpoints, and second, to analyze their own recognition levels thinking of their health conditions and the relationships between the supporting environments of old family dependants and their psychological healths and then finally, to propose suggestions being able to be helpful for living comfortably in an old age and thereby, building up good family relation. The statistical techniques used to analyze 115 respondents living in Puchun city are frequency, $x^2$ test, t-Jest, ANOVA, Pearson's Correlation Coefficiency and Regression analysis (SPSS package), pertnent to prove the hypothesis suggested in this paper. Of course, it is needless to say that more data are needed on this point. However, several main research findings can be summarized as follows: First, the better single aged persons may be in the habit of eating a meal and the higher they may think of their physical health conditions and movement, the more they want to participate in economic activities to be free from economical dependence upon their children and to overcome lonliness. Second, single aged women appear to have had higher ability to take care their health for themself than single aged men do. It is why signle aged women do not, in general, have big problems to manage their health. But, as shown in this paper, single aged person"s were more liable to the diseases of the aged and, thereby, requiring special medical treatment badly to be healthy. Third. single aged persons revealed potential desires to free themself from socio economic dependence upon their children even in simple labor Job which can draw a monthly salary of about W200, 000. Fourth, they are generally satisfied with their children's filial piety toward them. Nonethless, most of them appear to be reluctantly dependent upon their children and live lonly lives very much. Fifth, they seem to have some hesitation in expressing their candid opinions as that then are some others along with family environmental factors for psychological and emotiona stability. Accordingly, it is safe to conclude by saying that much attention should' be paid no only to socio-economic supports and better medical services for the aged but also to political supports of the society and towards their children for the aged's emotiona support, for improving the quality of their lives in old age and promoting efficiency in suporting for old family dependants.

• 한국환경과학회지
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• 제13권3호
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• pp.301-311
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• 2004

The purpose of this study was to examine teachers’ awareness of chemical waste produced in elementary school laboratory experimentation and how this awareness relates to collection and disposal of chemical waste. More specifically, the study looked at the correlation between the handling of chemical waste and factors such as years of teachers’ educational career, class size and amount of waste produced. The target population were 250 elementary school teachers in Gyeongnam area and 237 subjects were responded. Among the 237 responses, 37 cases that did not complete the questionnaire were eliminated. Therefore, 200 responses were analyzed in this study. The survey questionnaire consisted of 15 questions. The categories of the questionnaire were their skills of management and treatment of the chemical waste. The data collected were analyzed by SPSS 10.0, and the relations among variables such as class sizes and years of teaching experience were also analyzed by $x^2-test.$ The results in this study were as follows: First, there were no significant differences between the years of teaching and class sizes in the training experience of chemical waste disposal. Second, there was a significant difference between the science laboratory size and class sizes in the laboratory actual condition. In addition, in the relations between the number of times of experimentation and the years of teaching experience, there was a significant difference. Third, in terms of the discharge amount of chemical waste, there was a significant difference between the years of teaching and class sizes. Fourth, in the simple chemistry waste disposal process in the science laboratory, there also was a significant difference between the kinds of experimental equipments that used in the experimentation and the years of teaching. Based on this study, it was found that great amount of the chemical wastes produced in the science laboratory dumped into the drain and the treatment process of chemical waste was also inattentive. Even the importance of environmental education is emphasized in the elementary education, the basic problems occurred in the science laboratory is disregarded. Therefore, not only students but teachers have to pay attention to the disposal process of chemical waste in the laboratory in order to prevent environment pollution. Furthermore, the efforts of preventing environment pollution are needed such as opening the teacher training course about environment education, minimal use of chemicals, treatment of chemical waste, and so forth.

• 한국식물병리학회지
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• 제1권2호
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• pp.136-140
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• 1985

옥수수 위축모자이크바이러스병의 저항성품종 육성을 위한 기초자료를 얻고자 8개 옥수수 자식계통을 공시하여 이면교배에 의하여 조합능력검정 및 유전자의 분포상태를 추정한 요약하면 다음과 같다. 28개 $F_1$ 잡종조합의 본 바이러스에 대한 반응은 다양하여 이병도(1-4)의 조사결과 1.87에서 3.63까지 변이의 폭을 보였다. 조합능력의 분산분석결과 일반조합능력은 고도의 유의성이 인정되었으며, 특정조합능력은 유의성이 확정되지 않았다. 일반조합능력의 효과는 A632와 KS15가 부의 효과를 나타내어 이들 계통을 모본으로 사용하였을 때 잡종조합에 있어서 저항성을 증대시킬 수 있을 것으로 기대되었다. 특정조합능력의 효과는 A632와 KS5의 조합에서 가장 높은 부의 값을 나타내었으며 몇 개의 조합에서 부의 효과를 나타내었다. 회귀분석에 의한 유전자의 분포상태조사에서 저항성계통에는 우성유전자가 이병성계통에는 열성유전자가 많이 존재하는 것으로 나타났다.

• 한국식품과학회지
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• 제48권4호
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• pp.361-365
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• 2016

본 연구는 왕겨에서 tricin 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether(TTGE)를 생성시키는 균주를 분리와 동정하기 위하여 실시하였다. 발아 벼 추출물을 MYP, PDA, YM 및 LB 배지에 배양하고 각 배지에서 분리한 균을 왕겨에 접종해 TTGE 생성량을 측정한 결과 PDA 배지에서 자란 균에 의한 TTGE의 생성량($339.30{\mu}g/g$)이 가장 많았다. 분리한 균을 동정한 결과 Burkholderia vietnamiensis로 확인하였으며, 형태학적 및 생물학적 특성을 살펴본 결과 catalase 활성을 갖는 short rod 형태의 Gram 세균인 것으로 확인해 기존 Burkholderia 속의 특성과 일치하였다. 또한 분리한 세균을 살균한 왕겨에 접종하여 배양시킨 결과 배양기간에 따라 TTGE의 함량은 증가하였으며, 72시간 동안 배양에서 $435.86{\mu}g/g$로 가장 많은 증가를 나타내었다. 이러한 결과는 폐기되는 왕겨를 활용함으로써 왕겨의 함유된 TTGE의 함량을 증대시켜 기능소재로 사용할 수 있는 기초 자료가 될 것으로 판단된다.

• Clinical and Experimental Reproductive Medicine
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• 제30권3호
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• pp.203-206
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• 2003

Objectives: Controversial arguments exists on both the case for and against on the accumulation of mitochondrial DNA (mtDNA) deletion in association to tissue and age. The debate continues as to whether this mutation is a major contributor to the phenotypic expression of aging and common degenerative diseases or simply a clinical insignificant epiphenomenon. The objective of this study was to determine whether the accumulation of mtDNA deletion is correlated with age-related and tissue-specific variation. Materials and Methods: One hundred and fifty-seven tissues from blood, ovary, uterine muscle, and abdominal muscle were obtained from patients ranging in age from 31$\sim$60 years. After reviewing the clinical reports, patients with mitochondrial disorder were excluded from this study. The tissues were obtained at gynecological surgeries with the consent of the patient. Total DNA isolated from blood, ovary, uterine muscle, and abdominal muscle was amplified by two rounds of PCR using two pairs of primers corresponding to positions 8225-8247 (sense), 13551-13574 (antisense) for the area around deleted mtDNA and 8421-8440 (sense), 13520-13501 (antisense) for nested PCR product. A statistical analysis was performed by $x^2$-test. Results: About 0% of blood, 94.8% of ovary, 71.4% of uterine muscle, and 86.1% abdominal muscle harbored mtDNA deletion. When we examined the proportion of deleted mtDNA according to age deletion rate was 90% of ovary, 63.6% of uterine muscle, 77.7% of abdominal muscle in thirties and 100% of all tissue in fifties. Conclusion: The findings of this study suggest that the mtDNA deletion is varied in tissue-specific pattern and increases with aging.

• 한국정보과학회논문지:소프트웨어및응용
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• 제30권3_4호
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• pp.278-284
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• 2003

화행(speech act)이란 화자의 발화를 통해 나타나는 화자의 의도를 가르키며 자연어로 된 발화를 이해하고 이에 대한 응답을 생성하기 위해 중요한 요소이다. 본 논문에서는 한국어 화행 결정의 성능을 높이기 위해 두 단계 방법을 제안한다. 첫 번째 단계는 형태소 분석결과만을 이용하여 추출된 문장자질과 이전 화행을 이용하여 추출된 문맥자질 중 정보량이 높은 자질을 선택하는 단계이다. 이 단계에서는 형태소 분석 시스템을 사용하여 전체 자질을 구성하고 문서분류 분야의 자질 선택에서 높은 성능을 보인 카이제곱 통계량을 이용하여 효과적인 자질 선택한다. 두 번째 단계는 선택된 자질과 신경망을 이용하여 화행을 분석하는 단계이다. 본 논문에서 제시한 방법은 형태소 분석 결과만을 이용하여 자동적으로 화행을 결정할 수 있는 가능성을 제시하였으며 효과적인 자질 선택을 통해 자질의 수를 감소시키고 정보량이 높은 자질을 사용하여 속도와 성능을 향상 시켰다 본 논문은 제안된 시스템을 실제 영역에서 수집되어 전사된 10,285개의 발화와 17개의 화행으로 이루어진 대화 코퍼스에 대해 실험하였다. 본 논문은 이 코퍼스에서 8,349개 발화를 학습 코퍼스로 사용하여, 실험 코퍼스의 1,936개 발화에 대해 1,709개에 대해 정확한 화행을 제시하여, 88.3%의 정확도를 보였다. 이는 자질 선택을 하지 않았을 때 보다 약 8%가 증가된 결과이다.