• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.1
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• pp.61-64
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• 2000

A series of 5-oxaproline peptide derivatives was synthesized and evaluated for its ability to inhibit the prolyl 4-hydroxylase in vitro. Structure-activity studies show that the 5-oxaproline sequences, prepared by the 1,3-dipolar cycloaddition of the C-methoxycarbonyl-N-mannosyl nitrone in the presence of the ethylene, are more active than the corresponding proline derivatives. Prolyl 4-hydroxylase belongs to a family of $Fe^{2+}-dependent$ dioxygenase, which catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in -Gly-Xaa-Pro-Gly- of procollagen chains. In this paper we discover the more selective N-Cbz-Gly-Phe-Pro-Gly-OEt $(K_m\;=\;520\;{\mu}M)$ sequences which are showed stronger binding than others in vitro. Therefore, we set out to investigate constrained tetrapeptide that was designed to mimic the proline structure of pep tides for the development of prolyl 4-hydroxylase inhibitor. From this result, we found that the most potent inhibitor is N-Dansyl-Gly-Phe-5-oxaPro-Gly-OEt $(K_i\;=\;1.6\;{\mu}M)$. This has prompted attempts to develop drugs which inhibit collagen synthesis. Prolyl 4-hydroxylase would seem a particularly suitable target for antifibrotic therapy.

• Journal of the Institute of Convergence Signal Processing
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• v.15 no.2
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• pp.48-54
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• 2014

For the element ${\alpha}{\in}GF(p^n)$, two kinds of bases are known. One is a conventional polynomial basis of the form $\{1,{\alpha},{\alpha}^2,{\cdots},{\alpha}^{n-1}\}$, and the other is a normal basis of the form $\{{\alpha},{\alpha}^p,{\alpha}^{p^2},{\cdots},{\alpha}^{p^{n-1}}\}$. In this paper we consider the method of generating normal bases which construct the finite field $GF(p^n)$, as an n-dimensional extension of the finite field GF(p). And we analyze the code sequence generating algorithm and derive the implementation functions of code sequence generator based on the normal bases. We find the normal polynomials of degrees, n=5 and n=7, which can generate normal bases respectively, design, and construct the code sequence generators based on these normal bases. Finally, we produce two code sequence groups(n=5, n=7) by using Simulink, and analyze the characteristics of the autocorrelation function, $R_{i,i}(\tau)$, and crosscorrelation function, $R_{i,j}(\tau)$, $i{\neq}j$ between two different code sequences. Based on these results, we confirm that the analysis of generating algorithms and the design and implementation of the code sequence generators based on normal bases are correct.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.2
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• pp.167-172
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• 2001

The vitellin of firefly, Pyrocoelia rufa, is composed of three polypeptides, designated Vn1 (175 kDa), Vn2 (160 kDa) and Vn3 (45 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph, ovary and egg extracts, but not observed in the male. This vitellin was purified from the eggs of P. rufa by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In nature, vitellin of P. rufa has molecular weight of 400 kDa. Western blot analysis using polyclonal antiserum against purified vitellin showed that the antiserum was reacted with the three polypeptides, Vnl, Vn2 and Vn3 from the female adult hemolymph, ovary and egg extracts. Amino acid residues at N-terminus of three subunits were sequenced. The N-terminal sequences of large subunits, Vnl and Vn2, were similar to each other, But, the N-terminal sequences of small subunits Vn3, did not have any signnificant homology with large subunits.

• Korean Journal of Food Science and Technology
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• v.40 no.1
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• pp.70-75
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• 2008

Two extracellular amylase isozymes were purified and characterized from alkalophilic Pseudomonas sp. KFCC 10818 for the production of maltooligosaccharides. The molecular weights of the homogeneous proteins were 50 kDa and 75 kDa, respectively. The 50 and 75 kDa amylases showed optimum temperatures at 35 and $40^{\circ}C$, respectively. The optimum pH of the enzymes ranged from pH 6-8, and the enzymes were resistant to an alkaline condition of pH 12. Via the enzyme's actions, the final products from maltooligosaccharides or soluble starch were maltose and maltotriose. Calcium was a potent activator of the 50 kDa amylase. Finally, the N-terminal amino acid sequences of the 50 and 75 kDa amylases were QTVPKTTFV and DTVPGNAFQ, respectively.

• Journal of the Chungcheong Mathematical Society
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• v.22 no.4
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• pp.789-797
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• 2009

Invertible transformations over n-bit words are essential ingredients in many cryptographic constructions. When n is large (e.g., n = 64) such invertible transformations are usually represented as a composition of simpler operations such as linear functions, S-P networks, Feistel structures and T-functions. Among them we will study T-functions which are probably invertible transformation and are very useful in stream ciphers. In this paper we will show that $f(x)=x+(g(x)^2{\vee}C)$ mod $2^n$ is a permutation with a single cycle of length $2^n$ if both the least significant bit and the third significant bit in the constant C are 1, where g(x) is a T-function.

• Honam Mathematical Journal
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• v.42 no.2
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• pp.345-358
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• 2020

In this paper, we study the concepts of Wijsman lacunary invariant convergence, Wijsman lacunary invariant statistical convergence, Wijsman lacunary ${\mathcal{I}}_2$-invariant convergence (${\mathcal{I}}^{{\sigma}{\theta}}_{W_2}$), Wijsman lacunary ${\mathcal{I}}^*_2$-invariant convergence (${\mathcal{I}}^{\ast}^{{\sigma}{\theta}}_{W_2}$), Wijsman p-strongly lacunary invariant convergence ([W₂N_σθ]_p) of double sequence of sets and investigate the relationships among Wijsman lacunary invariant convergence, [W₂N_σθ]_p, ${\mathcal{I}}^{{\sigma}{\theta}}_{W_2}$ and ${\mathcal{I}}^{\ast}^{{\sigma}{\theta}}_{W_2}$. Also, we introduce the concepts of ${\mathcal{I}}^{{\sigma}{\theta}}_{W_2}$-Cauchy double sequence and ${\mathcal{I}}^{\ast}^{{\sigma}{\theta}}_{W_2}$-Cauchy double sequence of sets.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.446-455
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• 2018

This study examined the effects of different performance sequences of aerobic and strength exercises for 10 weeks on the body composition, physical function, and hormones in males aged in their 20s. A total of 30 subjects (N=30) were assigned randomly to either aerobic and resistance exercise (n=10, A+R), resistance and aerobic exercise (n=10, R+A), or a control group (n=10, CON). The different order of aerobic (HRmax 50-80%, 30 min) and resistance (50-80% 1RM, 30 min) exercises for 10 weeks was consisted of 3 times per week and 80 minutes per session. The body composition, physical function, and hormones were measured before and after the training period. The lean body mass (p=.015) was increased and the fat mass (p=.042) and % body fat (p=.007) in the A+R were decreased. The skeletal muscle mass (p=.001) in the R+A was increased and % body fat was decreased (p=.003). The weight (p=.03) and % body fat (p=.039) in the CON were increased. The aerobic capacity (p=.011) and muscular endurance (p=.001) in the A+R group were improved. The muscular endurance (p=.0016) in the R+A was improved. The epinephrine (p=.048), norepinephrine (p=.013), and cortisol (p=.045) levels in the A+R group were increased. The epinephrine (p=.046) level in the R+A group was increased. The insulin (p=.007) level increased in the CON group. In conclusion, both A+R and R+A groups produced superior results to the CON group but the A+R group was slightly more efficient than the R+A group.

• Communications for Statistical Applications and Methods
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• v.8 no.1
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• pp.229-235
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• 2001

Let {X$\_$n/, n$\geq$1] be a stationary sequence of negatively associated random variables with distribution function F(x)=P(X$_1$$\leq$x). The empirical distribution function F$\_$n/(x) based on X$_1$, X$_2$,....., X$\_$n/ is proposed as an estimator for F$\_$n/(x). Strong consistency and asymptotic normality of F$\_$n/(x) are studied. We also apply these ideas to estimation of the survival function.

• The Journal of Korean Society of Virology
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• v.28 no.4
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• pp.303-316
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• 1998

An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal transmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compared the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residues in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.434-437
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• 2003

A new bacterial strain, was isolated from activated sludge, identified and named P. aeruginosa EMS1. The new strain produced surface-active rhamnolipids by batch cultivation in mineral salts medium with waste flying oils. The mutant strain KH7, designated P. aeruginosa EMS1, derived by random mutagenesis with N-methyl-N-nitro-N-nitrosogoanidine treatment producing high levels of the biosurfactants was selected by an ion-pair plate assay. The mutant strain KH7 showed 4-5 times more hydrocarbon emulsification as compared to the parent when grown on waste frying oils and various hydrocarbons. Furthermore, P. aeruginosa EMS1 and mutant strain KH7 was also able to use whey as a co-substrate for growth and biosurfactant production. As results of this study, mutant strain KH7 is a very efficient biosurfactant producer, and its culture conditions are relatively inexpensive and economical. Rhamnolipid is synthesized by the rhlAB-encoded rhamnosyltransferase. To be convinced of these genes, we performed PCR based on P. aeruginosa PAO1 whole-genome database. rhl gene cluster nucleotide and amino acid sequences were compared for both parent and mutant. Comparison of nucleotide sequence of rhlAB, there were usually terminal's codons exchange.