• Biotechnology and Bioprocess Engineering:BBE
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• 제5권1호
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• pp.61-64
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• 2000

A series of 5-oxaproline peptide derivatives was synthesized and evaluated for its ability to inhibit the prolyl 4-hydroxylase in vitro. Structure-activity studies show that the 5-oxaproline sequences, prepared by the 1,3-dipolar cycloaddition of the C-methoxycarbonyl-N-mannosyl nitrone in the presence of the ethylene, are more active than the corresponding proline derivatives. Prolyl 4-hydroxylase belongs to a family of $Fe^{2+}-dependent$ dioxygenase, which catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in -Gly-Xaa-Pro-Gly- of procollagen chains. In this paper we discover the more selective N-Cbz-Gly-Phe-Pro-Gly-OEt $(K_m\;=\;520\;{\mu}M)$ sequences which are showed stronger binding than others in vitro. Therefore, we set out to investigate constrained tetrapeptide that was designed to mimic the proline structure of pep tides for the development of prolyl 4-hydroxylase inhibitor. From this result, we found that the most potent inhibitor is N-Dansyl-Gly-Phe-5-oxaPro-Gly-OEt $(K_i\;=\;1.6\;{\mu}M)$. This has prompted attempts to develop drugs which inhibit collagen synthesis. Prolyl 4-hydroxylase would seem a particularly suitable target for antifibrotic therapy.

• 융합신호처리학회논문지
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• 제15권2호
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• pp.48-54
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• 2014

원소 ${\in}F(p)$에 대하여 두 종류의 기저함수가 알려져 있다. 통상적인 다항식 기저(polynomial bases)는 $\{1,{\alpha},{\alpha}^2,{\cdots},{\alpha}^{n-1}\}$로 이루어지고 이와 다르게 정규 기저(normal bases)는 $\{{\alpha},{\alpha}^p,{\alpha}^{p^2},{\cdots},{\alpha}^{p^{n-1}}\}$의 형태를 갖는다. 본 논문에서는 소수 p의 원소로 이루어지는 유한장 GF(p)상에서 n차원 벡터공간인 확대장 $GF(p^n)$을 이룰 수 있는 정규기저의 발생과 생성에 대하여 검토하고 정규기저를 기반으로 부호계열 발생알고리즘을 분석하여 발생기구성함수를 도출하였다. 차수 n=5와 n=7인 두 종류의 정규기저를 생성할 수 있는 정규다항식을 발견하고 부호계열 발생기를 설계 구성하였다. 마지막으로 Simulink를 이용하여 두 종류(n=5, n=7)의 부호계열 그룹을 발생시키고 발생된 부호계열간의 자기상관함수, $R_{i,i}(\tau)$와 상호상관함수, $R_{i,j}(\tau)$, $i{\neq}j$ 특성을 분석하였다. 이 결과로부터 정규기저를 이용한 부호계열 발생알고리즘의 분석, 그리고 부호계열 발생기 설계와 구성이 타당함을 확인하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.167-172
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• 2001

The vitellin of firefly, Pyrocoelia rufa, is composed of three polypeptides, designated Vn1 (175 kDa), Vn2 (160 kDa) and Vn3 (45 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph, ovary and egg extracts, but not observed in the male. This vitellin was purified from the eggs of P. rufa by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In nature, vitellin of P. rufa has molecular weight of 400 kDa. Western blot analysis using polyclonal antiserum against purified vitellin showed that the antiserum was reacted with the three polypeptides, Vnl, Vn2 and Vn3 from the female adult hemolymph, ovary and egg extracts. Amino acid residues at N-terminus of three subunits were sequenced. The N-terminal sequences of large subunits, Vnl and Vn2, were similar to each other, But, the N-terminal sequences of small subunits Vn3, did not have any signnificant homology with large subunits.

• 한국식품과학회지
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• 제40권1호
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• pp.70-75
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• 2008

두 개의 amylase를 호알칼리성 Pseudomonas sp. KFCC 10818의 배양액으로부터 정제하여 그 특성을 조사하였다. 정제된 효소의 분자량은 각각 50 kDa과 75 kDa이었다. 이 효소들의 최적반응온도는 각각 $35^{\circ}C$와 $40^{\circ}C$였으며 50 kDa의 효소는 칼슘이온에 의하여 효소활성이 두 배로 촉진되었다. 이 두 효소는 최적 pH가 6-8 부근이었으며 pH 12의 조건에서도 효소활성을 유지하는 알칼리내성을 나타냈다. Maltooligosaccharide이나 soluble starch로부터 maltose와 maltotriose를 최종 효소반응산물로 생산하였다. 두 amylase는 N-말단 아미노산 서열이 각각 QTVPKTTFV와 DTVPGNAFQ로 분석되었다.

• 충청수학회지
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• 제22권4호
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• pp.789-797
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• 2009

Invertible transformations over n-bit words are essential ingredients in many cryptographic constructions. When n is large (e.g., n = 64) such invertible transformations are usually represented as a composition of simpler operations such as linear functions, S-P networks, Feistel structures and T-functions. Among them we will study T-functions which are probably invertible transformation and are very useful in stream ciphers. In this paper we will show that $f(x)=x+(g(x)^2{\vee}C)$ mod $2^n$ is a permutation with a single cycle of length $2^n$ if both the least significant bit and the third significant bit in the constant C are 1, where g(x) is a T-function.

• 호남수학학술지
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• 제42권2호
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• pp.345-358
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• 2020

In this paper, we study the concepts of Wijsman lacunary invariant convergence, Wijsman lacunary invariant statistical convergence, Wijsman lacunary ${\mathcal{I}}_2$-invariant convergence (${\mathcal{I}}^{{\sigma}{\theta}}_{W_2}$), Wijsman lacunary ${\mathcal{I}}^*_2$-invariant convergence (${\mathcal{I}}^{\ast}^{{\sigma}{\theta}}_{W_2}$), Wijsman p-strongly lacunary invariant convergence ([W₂N_σθ]_p) of double sequence of sets and investigate the relationships among Wijsman lacunary invariant convergence, [W₂N_σθ]_p, ${\mathcal{I}}^{{\sigma}{\theta}}_{W_2}$ and ${\mathcal{I}}^{\ast}^{{\sigma}{\theta}}_{W_2}$. Also, we introduce the concepts of ${\mathcal{I}}^{{\sigma}{\theta}}_{W_2}$-Cauchy double sequence and ${\mathcal{I}}^{\ast}^{{\sigma}{\theta}}_{W_2}$-Cauchy double sequence of sets.

• 한국산학기술학회논문지
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• 제19권1호
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• pp.446-455
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• 2018

본 연구는 20대 남성을 대상으로 10주간 저항운동과 유산소 운동을 순서가 다르게 실시한 후 신체조성, 운동수행능력 및 호르몬의 변화에 어떠한 영향을 주었는지에 관한 연구이다. 실험 참가자들 (N=30)은 무선할당 방식으로 유산소 운동 후 저항 운동그룹 (n=10, A+R), 저항운동 후 유산소 운동그룹(n=10, R+A)과 통제그룹 (n=10, CON)으로 나누었다. 10주간 유산운동(최대심박수 50-80%, 30분)과 저항운동(50-80% 1RM, 30분)으로 순서를 달리하여 주3회 한 회당 총 70-80분으로 운동을 수행하였다. 훈련 기간 전과 10주후에 신체조성, 운동수행능력, 호르몬이 측정되었다. 연구 결과는 신체조성에서 A+R의 골격근량(p=.015)은 증가하였고 체지방량(p=.042), 체지방률(p=.007)은 감소하였다. R+A의 골격근량(p=.001)은 증가하였고, 체지방량(p=.003)은 감소하였다. CON은 체중(p=.03)과 체지방량(p=.039)이 증가하였다. 운동수행능력에서 A+R에 유산소능력(p=.011)과 저항성능력(p=.001)이 증가하였고, R+A는 저항성능력(p=.0016)이 증가하였다. 호르몬에서 A+R에 에피네프린(p=.048), 노르에피네프린(p=.013), 코티졸(p=.045)이 증가하였고, R+A는 에피네프린(p=.046)이 증가하였다. CON에서는 인슐린(p=.007)이 증가하였다. 이러한 결과로 보면 두 운동 그룹(A+R & R+A)이 통제그룹(CON)에 비하여 우수하고, 그리고 두 운동 그룹 중에서는 A+R이 R+A보다 좀 더 효과적임을 나타내었다.

• Communications for Statistical Applications and Methods
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• 제8권1호
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• pp.229-235
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• 2001

Let {X$\_$n/, n$\geq$1] be a stationary sequence of negatively associated random variables with distribution function F(x)=P(X$_1$$\leq$x). The empirical distribution function F$\_$n/(x) based on X$_1$, X$_2$,....., X$\_$n/ is proposed as an estimator for F$\_$n/(x). Strong consistency and asymptotic normality of F$\_$n/(x) are studied. We also apply these ideas to estimation of the survival function.

• 대한바이러스학회지
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• 제28권4호
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• pp.303-316
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• 1998

An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal transmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compared the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residues in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.434-437
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• 2003

A new bacterial strain, was isolated from activated sludge, identified and named P. aeruginosa EMS1. The new strain produced surface-active rhamnolipids by batch cultivation in mineral salts medium with waste flying oils. The mutant strain KH7, designated P. aeruginosa EMS1, derived by random mutagenesis with N-methyl-N-nitro-N-nitrosogoanidine treatment producing high levels of the biosurfactants was selected by an ion-pair plate assay. The mutant strain KH7 showed 4-5 times more hydrocarbon emulsification as compared to the parent when grown on waste frying oils and various hydrocarbons. Furthermore, P. aeruginosa EMS1 and mutant strain KH7 was also able to use whey as a co-substrate for growth and biosurfactant production. As results of this study, mutant strain KH7 is a very efficient biosurfactant producer, and its culture conditions are relatively inexpensive and economical. Rhamnolipid is synthesized by the rhlAB-encoded rhamnosyltransferase. To be convinced of these genes, we performed PCR based on P. aeruginosa PAO1 whole-genome database. rhl gene cluster nucleotide and amino acid sequences were compared for both parent and mutant. Comparison of nucleotide sequence of rhlAB, there were usually terminal's codons exchange.