• 핵의학기술
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• 제24권1호
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• pp.33-38
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• 2020

비타민D는 체내에서 칼슘 대사를 조절하여 뼈의 건강을 유지하며, 세포의 증식 및 분화의 조절, 면역 기능 등에 관여하는 중요한 역할을 한다. 부족 시 구루병, 골다공증의 위험이 높아지고 심혈관계 질환, 당뇨병, 일부 암 등의 발병 위험이 증가하는 것으로 보고되고 있다. 특히 한국은 비타민D 부족의 인구 비율이 매우 높은 국가 중 하나이다. 따라서 비타민D 부족의 진단 및 치료를 위해 혈중 25-OH-VitD 또는 25-OH-VitD3의 정확한 측정이 요구된다. 이에 본원에서 시행하고 있는 25-OH-VitD와 25-OH-VitD3검사를 비교 평가하여 비타민D에 대한 정확한 진단 및 치료에 기여하고자 한다. 25-OH-VitD와 25-OH-VitD3의 결과를 전향적으로 측정하여 상관성, 재현성, 모집단 분포율을 구하였다. 또한 2017년 4월에서 2019년 6월까지 서울아산병원 핵의학과 혈액검사실에서 측정한 내부정도관리와 대한핵의학기술학회에서 주관하는 2018년 상반기, 하반기 외부정도관리(기관간 숙련도 평가)에 참여한 결과를 비교하였다. 그 결과 97개의 검체를 대상으로 25-OH-VitD는 25-OH-VitD3 × 0.9 + 0.3 (R >0.9)로 강력한 양의 상관관계를 보였다. 반복 측정을 통한 재현성 평가 결과 평균 diff(%) 값은 7.7%, 7.4%로 모두 우수한 성적을 나타내었다. 또한 모집단 분포를 비교한 결과 통계적으로 유의한 차이를 보이지 않았다(p>0.05). 2017년 4월에서 2019년 6월까지 서울아산병원 핵의학 혈액검사실에서 측정한 내부정도관리 결과값은 평균(CV%) 6.2%, 6.8%을 나타내었다. 2018년 상반기 외부정도관리(기관간 숙련도 평가) 수행도 평가 결과 Z 값이 25-OH-VitD는 저, 중, 고농도에 대해 -1.10, -1.00, -0.90이며 25-OH-VitD3는 0.22, 0.47, 1.10이고 하반기 수행도 평가 결과는 각각 -0.80, -0.30, -0.10과 -0.01, 1.50, 1.30으로 모두 Z ≤ 2.0의 적합한 결과를 얻었다. 방사면역 측정법에 의한 25-OH-VitD와 25-OH-VitD3 검사의 비교 평가 결과 상관성, 재현성, 모집단 분포율, 내부정도관리, 외부정도관리(기관간 숙련도 평가) 모두 우수한 성적을 보였다. 따라서 혈액 내 25-OH-VitD 또는 25-OH-VitD3로 단독 측정이 가능한 방사면역 측정법은 비타민D 부족에 대한 스크리닝 검사로 적합한 것으로 사료된다.

• Tuberculosis and Respiratory Diseases
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• 제78권3호
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• pp.232-238
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• 2015

Background: A relationship between low vitamin D levels and the development or outcomes of respiratory diseases has been identified. However, there is no data on the vitamin D status in patients with acute eosinophilic pneumonia (AEP). We evaluated the vitamin D status in patients with AEP among South Korean military personnel. Methods: We prospectively compared the serum levels of total 25-hydroxyvitamin D [25(OH)D], 25(OH)D3, and 25(OH)D2 among patients with AEP, pulmonary tuberculosis (PTB), and community-acquired pneumonia (CAP). Results: In total, 65 patients with respiratory diseases, including AEP (n=24), PTB (n=19), and CAP (n=22), were identified. Of the 24 patients with AEP, 2 (8%) had deficient total 25(OH)D levels (<10 ng/mL), 17 (71%) had insufficient total 25(OH)D levels (${\geq}10$ to <30 ng/mL), and only 5 (21%) had sufficient total 25(OH)D levels (${\geq}30$ to <100 ng/mL). The difference in the total 25(OH)D levels among patients with AEP, PTB, and CAP was not statistically significant (p=0.230). The median levels of total 25(OH)D, 25(OH)D3, and 25(OH)D2 were 22.84, 22.84, and 0.00 ng/mL, respectively, and no differences in the 25(OH)D level were present among patients with AEP, PTB, and CAP with the exception of the total 25(OH)D level between patients with AEP and PTB (p=0.042). Conclusion: We have shown that low vitamin D levels are frequently found in patients with AEP and are comparable with those in patients with PTB and CAP.

• Journal of Nutrition and Health
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• 제39권7호
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• pp.649-660
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• 2006

This study was performed to estimate serum 2S-hydroxyvitamin D (25-OHD) level in wintertime and to evaluate the relationship between serum 25-OHD level and associated factors in 50 premenopausal working women aged 30-49 y in Busan. The serum 25-OHD level was measured by radioimmunoassay. Data for physiological characteristics, lifestyle factors, physical activity and nutrient intake was assessed by questionnaire including information about outdoor activity time, daily activity diary and 24hr recall method. The mean vitamin D intake was $4.24{\mu}g$, which corresponded to 84.9% of the Korean RDA. The mean level of serum 25-OHD was 25.7 ng/mL. Vitamin D deficiency (25-OHD < 7nmol/L) and toxicity $(25-OHD{\geq}75\;nmol/L)$ were not found in the subjects. However, the prevalence of vitamin D insufficiency (25-OHD < 15 nmol/L) and hypovitaminosis D (25-OHD < 30 nmol/L) were 12% and 60% respectively. The serum 25-OHD level showed positive significant correlations with the duration of outdoor activity per weekdays (p < 0.05). Our findings suggest that hypovitaminosis D was common in the subjects in wintertime. So nutritional education for increasing outdoor activities is needed for premenopausal working women to increase vitamin D status in wintertime.

• 대한영양사협회학술지
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• 제23권1호
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• pp.39-53
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• 2017

Insufficient vitamin D intake is a major health problem around the world. Recently, many studies have suggested that vitamin D intake may influence insulin resistance. However, little is known about the association between vitamin D and diabetes mellitus. The aim of this study was to investigate the association between serum 25-hydroxy vitamin D (25(OH)D) levels and diabetes mellitus in Korean adults. This study was a cross-sectional analysis of 3,686 participants of the Korean National Health and Nutrition Examination Survey (KNHANES) 2013~2014 aged 19 years and higher. The results showed that the mean serum 25-hydroxy vitamin D (25(OH)D) level in Korean adults was 16.77 ng/mL, and 74.2% of them had an insufficient serum 25-hydroxy vitamin D (25(OH)D) level (below 20 ng/mL). In normal and pre-diabetic groups, the serum 25-hydroxy vitamin D (25(OH)D) level significantly increased with age (P<0.001), but there was no significant difference relative to age in the diabetic group. Low vitamin D levels (25-hydroxy vitamin D < 10 ng/mL) were associated with increased fasting blood glucose levels, compared with optimal vitamin D levels (25-hydroxy vitamin D > 30 ng/mL), after adjusting for variables that may affect fasting blood glucose, but this result was not significant. In conclusion, although no significant association was observed between diabetes prevalence and vitamin D levels in this study, further studies are needed because the effect of vitamin D on diabetes remains controversial. This nutrient plays a crucial role in the body, and levels are insufficient in the Korean population.

• Journal of Nutrition and Health
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• 제29권7호
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• pp.747-757
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• 1996

In this study, the serum level of 25-hydroxyvitamin D(25-(OH)D) was measured by high pressure liquid chromatography(HPLC), and factors affecting it were investigated in 72 young adults age ranging from 21 years to 39 years with normal bone density. The mean level of serum 25-(OH)D was 20.0$\pm$6.8ng/ml in males and 26.1$\pm$12.3ng/ml in females, which was significantly higher in females (p<0.01). The serum level of parathyroid hormone(PTH) showed a negative correlation with that of 25-(OH)D(p<0.05). Time spent outdoors in a day correlated positively with the serum level of 25-(OH)D(p<0.01). During the day, a specific time between 12:00 a.m. and 2:00 p.m. showed the most significant correlation with the level of 25-(OH)D(p<0.005). Among the nutrients studied, fat and vitamin D intake were positively correlated with the serum 25-(OH)D level. Stepwise multiple regression analysis showed that the serum level of 25-(OH)D could be fit by vitamin D intake(34.7% explained), serum PTH level (27.3% explained) and the time spent outdoors during the specific time(28.4% explained).1996)

• 한국산림과학회지
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• 제101권4호
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• pp.648-655
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• 2012

본 연구는 치유의 숲의 치유효과 증진을 위해 대중의 시각적 선호도에 기반 한 적정 임목밀도 관리 방향을 제시하는데 그 목적이 있다. 잣나무, 편백, 소나무, 낙엽송의 4개 침엽수종에 대해서 3개 주요 직경급별로 임목밀도 시뮬레이션을 이용하여 시각적 선호도를 조사 분석하였다. 그 결과, 잣나무의 경우, 일반소경재(D<25)는 임목밀도 약 816본/ha, 우량중경재($25{\leq}D$ <40)는 임목밀도 약 400본/ha, 우량대경재($40{\leq}D$)는 임목밀도 약 277본/ha을 가장 높게 선호하였다. 편백의 경우, 일반소경재(D<25)는 임목밀도 약 625본/ha, 우량중경재($25{\leq}D$ <40)는 임목밀도 약 625본/ha, 우량대경재($40{\leq}D$)는 임목밀도 약 400본/ha의 선호도가 가장 높게 나타났다. 소나무의 경우, 일반소경재(D<25)는 임목밀도 약 816본/ha, 우량중경재($25{\leq}D$ <40)는 임목밀도 약 625본/ha, 우량대경재($40{\leq}D$)는 임목밀도 약 400본/ha의 선호도가 가장 높게 나타났다. 낙엽송의 경우, 일반소경재(D<25)는 임목밀도 약 625본/ha, 우량중경재($25{\leq}D$ <40)는 임목밀도 약 625본/ha, 우량대경재($40{\leq}D$)는 임목밀도 약 277본/ha을 가장 선호하는 것으로 나타났다.

• 한국식품영양과학회지
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• 제27권1호
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• pp.141-148
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• 1998

This study describes the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3, calcitriol] analog, 1,25(OH)2-16ene-23yne-D3 on proliferatin and differentiatin of the human histiocytic lymphoma cell line U937. This paper also describes the effects of 1,25(OH)2-16ene-23yne-D3 on ${\gamma}$-interferon(IFN-${\gamma}$) synthesis by phytohemagglutinin-activated peripheral blood lymphocytes(PBLs). In the present investigation, 1,25(OH2)-16ene-23yne-D3 was compared to the natural metablite of vitamin D3, 1,25(OH)2D3. 1,25(OH)2-16ene-23yne-D3 was more potent than 1,25(OH)2D3 for inhibition of proliferation and induction of differentiation of U937 cells, Its effects on inhibition of proliferation was about 30-fold more potent than 1,25(OH)2D3. On induction of differentiation as measured by nonspecific esterase (NSE) activity and morphologic change, this analog morphologically and functionally differentiated U937 cells to monocyte-macrophage phenotype showing a decrease of N/C ration in Giemsa staining and the increase of adherence ability of surface. After 3 days in culture, a more significant supression of IFN-${\gamma}$ synthesis analog on supression of IFN-${\gamma}$ synthesis was a dose-dependent manner, with peak activity at 10-7M. The strong direct effects of 1,25(OH)2-16ene-23yne-D3 on cell proliferation and cell differentiation, make this compound an interesting candidate for clinical studies for several types of malignancies, and the effects on supression of IFN-${\gamma}$ synthesis provide the further evidence for a role of 1,25(OH)2-16ene-23yne-D3 in immunoregulation.

• Nutrition Research and Practice
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• 제9권2호
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• pp.158-164
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• 2015

BACKGROUND/OBJECTIVES: Vitamin D deficiency is common in hemodialysis patients. The aim of this study was to identify whether or not sun exposure and dietary vitamin D intake have effects on serum 25-hydroxyvitamin D (25(OH)D) status in hemodialysis (HD) patients. The objective was to identify the main determinants of serum vitamin D status in the study subjects. SUBJECTS/METHODS: A cross-sectional study of 47 HD patients (19 males and 28 females) was performed. We assessed serum 25(OH)D and $1,25(OH)_2D$ levels between August and September 2012 and analyzed the prevalence of vitamin D deficiency in HD patients. To evaluate the determinants of serum 25(OH)D levels, we surveyed dietary vitamin D intake, degree of sun exposure, and outdoor activities. To compare biological variables, serum 25(OH)D was stratified as below 15 ng/ml or above 15 ng/ml. RESULTS: Mean 25(OH)D and $1,25(OH)_2D$ levels were $13.5{\pm}5.8ng/ml$ and $20.6{\pm}11.8pg/ml$, respectively. The proportions of serum 25(OH)D deficiency (< 15 ng/ml), insufficiency (15-< 30 ng/ml), and sufficiency (${\geq}30ng/ml$) in subjects were 72.4%, 23.4%, and 4.3%, respectively. Prevalence of vitamin D deficiency in female patients was 78.6%, whereas that in males was 63.2% (P = 0.046). Vitamin D intake and sun exposure time were not significantly different between the two stratified serum 25(OH)D levels. Dietary intake of vitamin D did not contribute to increased serum 25(OH)D levels in HD patients. The main effective factors affecting serum 25(OH)D status were found to be the sun exposure and active outdoor exercise. CONCLUSIONS: Hypovitaminosis D is common in HD patients and is higher in females than in males. Sun exposure is the most important determinant of serum 25(OH)D status in HD patients.

• 대한지역사회영양학회지
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• 제10권1호
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• pp.79-90
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• 2005

This study was performed to estimate serum 25-hydroxyvitamin D level and to evaluate the relationship between serum 2S-hydroxyvitamin D level and associated factors. The subjects were 61 premenopausal working women aged 30 - 49 y in Busan. The serum 25-hydroxyvitamin D level was measured by radioimmunoassay. Data for physiological characteristics, lifestyle factors, physical activity and nutrient intake were assessed by questionnaire including information about outdoor activity time, daily activity diary and 24 hr recall method. The mean vitamin D intake was 3.12 ug, which corresponded to $62.5\%$ of the Korean RDA. The mean level of serum 25-hydroxyvitamin D was 31.0 ng/mL. Low 25-hydroxyvitamin D (<25 nmol/L) was not found in the subjects. The serum 2S-hydroxyvitamin D level showed positive significant correlations with exercise hours, daily energy expenditure, hours of outdoor activity per weekdays (p < 0.001, P < 0.05, P < 0.05). Exercise hours were found to be the most important determinant of serum 25-hydroxyvitamin D level. Therefore nutritional education for increasing hours of physical activity including indoor and outdoor exercise, is needed for premenopausal working women to increase vitamin D status.

• 대한치과교정학회지
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• 제27권2호
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• pp.335-347
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• 1997

교정치료시 치아이동은 치주인대세포를 매개로 골조직의 개조가 일어나는 과정으로서 골조직의 성장과 개조는 조골세포, 파골세포 및 그 전구세포의 증식, 분화 및 활성에 영향을 미치는 여러가지 전신적 인자와 국소적 인자에 의하여 조절되고 있다. 1.25-Dihydroxyvitamin $D_3$는 vitamin $D_3$의 대사물로서 경조직에 있어서 칼슘과 인산의 이동에 중요한 역할을 담당하고 있는 것으로 알려져 있으나 치주인대에 대한 1,25-Dihydroxyvitamin $D_3$의 생물학적 기능은 잘 알려져 있지 않다. 1.25-Dihydroxyvitamin $D_3$가 치주인대세포의 활성에 미치는 영향을 관찰하고자 10, 25, 50, 100ng/ml농도의 1,25 Dihydroxyvitamin $D_3$를 배양 치주인대세포에 첨가하여 배양 1, 2, 3일 후 M.T.T. 방법으로 세포의 활성을 관찰하였으며, 백서의 실험적 치아이동시 치주인대 내에 1,25-Dihydroxyvitamin $D_3$를 투여하여 12, 24, 36, 48, 72시간 및 7일 후의 조직 변화 소견을 관찰하여 다음과 같은 결과를 얻었다. 1. 10ng, 25ng/ml 농도의 1,25-Dihydroxyvitamin $D_3$를 치주인대세포에 가한 후 배양 1, 2, 3일째의 실험군 활성은 대조군과 차이가 없었다. 2. 50ng/ml 농도의 1,25-Dihydroxyvitamin $D_3$를 치주인대세포에 가한 후 배양 3일째의 활성은 대조군에 비하여 유의하게 증가되었으며 100ng/ml농도에서는 배양 2, 3일째에 유의하게 많았다. 3. 백서에 교정력을 가한 후 인장측에서의 골아세포 활성, 치주인대섬유의 파열, 모세혈관 증식은 투여후 7일까지 1.25-Dihydroxyvitamin $D_3$ 투여측과 대조측 간에 차이가 없었다. 4. 교정력을 가한 후 36시간부터 1,25-Dihydroxyvitamin $D_3$를 투여한 압박측의 파골세포 활성 및 치조골 흡수량이 증가하였다. 이상과 같은 결과로, 50-100ng/ml 농도에서 1,25-Dihydroxyvitamin $D_3$의 농도와 배양 기간에 비례하여 치주인대세포의 활성이 증가하였으며 1,25-Dihydroxyvitarnin $D_3$가 투여 36시간부터 파골세포의 활성과 그에 따른 치조골 흡수량 증가에 영향을 미쳤다고 사료된다.