• Journal of Animal Science and Technology
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• v.55 no.2
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• pp.87-93
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• 2013

We identified four new bovine tri-nucleotide microsatellite loci and analyzed their sequence structures and genetic parameters in 105 randomly selected Korean cattle (Hanwoo). Allele numbers of the loci B17S0808, B15S6253, B8S7996, and B17S4998 were 10, 11, 12, and 29, respectively. These alleles contained a simple or compound repeat sequences with some variations. Allele distributions of all these loci were in Hardy-Weinberg equilibrium (P > 0.05). Observed heterozygosity and expected heterozygosity ranged from 0.54 (B15S6253) to 0.92 (B17S4998) and from 0.599 (B15S6253) to 0.968 (B17S4998), respectively, and two measures of heterozygosity at each locus were highly correlated. Polymorphism information content (PIC) for these 4 loci ranged from 0.551 (B15S6253) to 0.932 (B17S4998), which means that all these loci are highly informative (PIC > 0.5). Other genetic parameters, power of discrimination (PD) and probability of exclusion (PE) ranged from 0.783 (B15S6253) to 0.984 (B17S4998) and from 0.210 (B15S6253) to 0.782 (B17S4998), respectively. Their combined PD and PE values were 0.9999968 and 0.98005176, respectively. Capillary electrophoresis revealed that average peak height ratio for a stutter was 13.89% at B17S0808, 26.67% at B15S6253, 9.09% at B8S7996, and 43.75% at B17S4998. Although the degree of genetic variability of the locus B15S6253 was relatively low among these four microsatellite markers, their favorable parameters and low peak height ratios for stutters indicate that these four new tri-nucleotide microsatellite loci could be useful multiplex PCR markers for the forensic and population genetic studies in cattle including Korean native breed.

• Development and Reproduction
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• v.15 no.3
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• pp.223-230
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• 2011

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants derived from incomplete combustion of carbons and crude oil. In this study, we investigated the effects of benzo[a]pyrene (B[a]P), a representative PAHs on in vitro sex steroid hormone production and germinal vesicle breakdown (GVBD) using isolated oocytes of longchin goby (Chasmichthys dolichognathus) and chameleon goby (Tridentiger trigonocephalus). Oocytes in diameters of 0.8-0.9 (end vitellogenic stage) and 0.9-1.0 mm (germinal vesicle migratory stage) from longchin goby and 0.5 mm (fully vitellogenic stage) from chameleon goby were used. In GVBD assay, B[a]P at 10 nM stimulated GVBD in the oocytes of 0.8-0.9 mm from longchin goby. B[a]P at 1 nM stimulated GVBD in the oocytes with diameter 0.5 mm from chameleon goby. In steroid production from oocytes of longchin goby, B[a]P at 100 nM decreased testosterone (T) production, B[a]P at 1,000 nM increased estraiol-17 (J (E2) production and 10 and 100 nM increased $17,20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$) production in the oocytes with diameter 0.8-0.9 mm. B[a]P at 1,000 nM increased E2 production, 100 and 1,000 nM increased $17{\alpha}20{\beta}P$ production in the oocytes with diameter 0.9-1.0 mm. In steroid production of oocytes from chameleon goby, B[a]P at 1,000 nM increased $E_2$ production. B[a]P at 10 nM increased $17{\alpha}20{\beta}P$ production. In the ratio of $E_2$ to T ($E_2$/T), B[a]P at 100 and 1,000 nM increased $E_2$/T in the oocytes of longchin goby. B[a]P at 100 nM also increased $E_2$/T in the oocytes of chameleon goby. Taken together, these results suggest that B[a]P have not only weak estrogenic effects but progestogenic effects on oocyte maturation.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.8
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• pp.1134-1142
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• 2008

Cationic amino acid transporter $b^{0,+}AT$ (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine $b^{0,+}AT$. Based on the sequence of porcine $b^{0,+}AT$ deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $b^{0,+}AT$ was isolated. The porcine $b^{0,+}AT$ cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse $b^{0,+}AT$, respectively. Real-time RT-PCR indicated porcine $b^{0,+}AT$ transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest $b^{0,+}AT$ mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest $b^{0,+}AT$ mRNA abundance while the colon had the lowest (p<0.05). The $b^{0,+}AT$ mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest $b^{0,+}AT$ expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).

• Journal of the Korean Magnetics Society
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• v.8 no.5
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• pp.271-274
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• 1998

Spin reorientation and magnetocrystalline anisotropy of magnetically aligned $(Nd_{1-x}R_x)_2Fe_{14}B$ (R=Y, Pr) power were studied. The spin reorientation temperature $(T_{SR})$ of $(Nd_{1-x}R_x)_2Fe_{14}B$ decreases linearly by increasing Pr-substitution with the ratio of ${\Delta}T_{SR}=-1.35$ K/Pr at.% in composition range of 0$\leq$x$\leq$0.75. The spin reorientation temperature of $(Nd_{1-x}R_x)_2Fe_{14}B$ decreases by increasing Pr-substitution to 118 K (x=0.5) then increases to 122 K (x=0.75). The spin reorientation angle at 4.2 K decreases by increasing rare earth substitution with the ratio of $\Delta$SRA=-0.073$^{\circ}$/Y at.% and $\Delta$SRA=-0.258$^{\circ}$/Pr at.% in composition range of 0$\leq$x$\leq$0.5. The spin reorientation is expected to disappear at x$\geq$0.9 in case of $(Nd_{1-x}R_x)_2Fe_{14}B$ and at x$\geq$0.8 in case of $(Nd_{1-x}R_x)_2Fe_{14}B$.

• Journal of Ecology and Environment
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• v.40 no.2
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• pp.84-88
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• 2016

To understand the effects of habitat characteristics on the genetic diversity of Persicaria thunbergii, three sites of different environmental conditions in a water system were surveyed. Site A was the closest to the source of the water system, and there was a dam between sites A and B. Site C is located on the lowest downstream in the water system. Vegetation survey of four quadrats at each site was performed, and soil samples were collected for physicochemical analysis. Random amplification of polymorphic DNA (RAPD) analysis of ten P. thunbergii individuals at each site was conducted to calculate population genetic diversity and genetic distance among populations. Soil was sterile sand at site A, whereas loamy soil at sites B and C. A pure stand of P. thunbergii appeared at site A, while other species occurred together (such as Humulus japonicus and Phragmites australis) at sites B (Shannon-Wiener index; $H_B=0.309$) and C ($H_C=0.299$). Similar to the species diversity, genetic diversity (Nei's gene diversity; h) within population of site A ($h_A=0.2381$) was relatively lower than sites B ($h_B=0.2761$) and C ($h_C=0.2618$). However, site C was separated from sites A and B in genetic distance rather than the geographical distance (Nei's genetic distance; A~B, 0.0338; B~C, 0.0685; A~C, 0.0833).

• Journal of the Korean Ceramic Society
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• v.34 no.4
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• pp.413-419
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• 1997

The specimens which were prepared from cokes with additions of 0~25 wt% B4C were sintered in Ar atmosphere at 220$0^{\circ}C$. The effects of B4C content on graphitization and oxidation resistance of cokes were investigated. B4C accelerates the graphitization of cokes and at 220$0^{\circ}C$ the degree of graphitization increased from 0.33 which is the value of pure carbon to 0.56, which increased bluk density and porosity. Especially bending strength increased as th graphitization temperature increased. Oxidation resistance property was greatly improved when B4C was added more than 10wt% at 80$0^{\circ}C$ and when B4C was added more than 20wt% at 100$0^{\circ}C$. This was because that the thin layer of B2O3 glass phase on the surface of the composite could be identified to increase the oxidation resistance.

• Journal of Radiation Protection and Research
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• v.6 no.1
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• pp.31-33
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• 1981

The 42 patients with carcinoma of the cervix, performed intracavitary radiotherapy, were analysed the doses at points A and B comparing to the mghr prescription. The doses at points A and B were calculated by PC-12 computer planning system. Correlation coefficienty between doses at points A and B and the mghr prescription are 0.82 (p<0.001) and 0.90 (p<0.001) respectively. The slope of the point A line is 0.70 and the slope of the point B is 0.21. Therefore, the dose at point A is approximately 3/4 the mghr prescription and the dose at point B is approximately 1/4 the mghr precription.

• The Journal of the Acoustical Society of Korea
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• v.28 no.5
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• pp.447-453
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• 2009

This paper introduces the method for detect voices and exact end point at low SNR by maximizing voice energy. Conventional VAD (Voice Activity Detection) algorithm estimates noise level so it tends to detect the end point inaccurately. Moreover, because it uses relatively long analysis range for reflecting temporal change of noise, computing load too high for application. In this paper, the SEM-VAD (Speech Energy Maximization-Voice Activity Detection) method which uses psycho-acoustical bark scale filter banks to maximize voice energy within frames is introduced. Stable threshold values are obtained at various noise environments (SNR 15 dB, 10 dB, 5 dB, 0 dB). At the test for voice detection in car noisy environment, PHR (Pause Hit Rate) was 100%accurate at every noise environment, and FAR (False Alarm Rate) shows 0% at SNR15 dB and 10 dB, 5.6% at SNR5 dB and 9.5% at SNR0 dB.

• Journal of the Korean Ceramic Society
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• v.36 no.1
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• pp.7-14
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• 1999

TiZrB2 solid solution was synthesized using fine powders of Ti, Zr and B by SHS microwave process. The characterization of the synthesized powder and sintered bodies ws investigated. The combustion temperature and rate were increased with increasing the mole ratio of Zr in temperature profile, and showed the maximum combustion temperature and velocity values of 285$0^{\circ}C$ and 14.6mm/sec in Ti0.2Zr0.8B2 composition. Phase separation has been occured into a composite with TiB2 and ZrB2 phases from TiZrB2 solid solution, which was hot pressed sintering at 30 MPa for an hour at 190$0^{\circ}C$. At the composition of Ti0.8Zr0.2B2 the best properties has been obtained in relative density, bending strength, fracture toughness and hardness, with 99%, 680 MPa, 7.3MPa.m1/2 and 2750 Kg/$\textrm{mm}^2$ respectively.

• The Journal of the Korea institute of electronic communication sciences
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• v.16 no.6
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• pp.1053-1058
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• 2021

This paper presents the multi-band mixer which converts a X-, K- and Ka-band adaptively by adjusting the gate-bias voltage of an active device. The proposed mixer presented a conversion loss of -10 dB at -0.8 V gate-bias voltage for X-band, a conversion loss of -9 dB at -0.3 V gate-bias voltage for K-band and for Ka-band, a conversion loss of -7 dB at -0.2 V gate-bias voltage under the LO power of +6.0 dBm. The 1dB compression point (P1dB) is +0.5 dBm for all band.