• Title/Summary/Keyword: $_14C-hexadecane$degradation rate

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Effect of temperature and salinity on the bacterial degradability of petroleum hydrocarbon (세균의 유류 분해능에 미치는 온도와 염분의 영향)

  • 오영숙;김상종
    • Korean Journal of Microbiology
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    • v.26 no.4
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    • pp.339-347
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    • 1988
  • The rate of bacterial degradation of hydrocarbon was estimated for the measurment of the self-purification capacity of the aquatic ecosystem. Strain ND601P-2, selected as petroleum degrading bacteria from Nakdong River Estuary with high degradability of petroleum, transformed 42% of hexadecane to $CO_{2}$ or cell mateials under the conditions of $25^{\circ}C$, 0.03M NaCl, 167mg-$NH_{4}^+/1, 950 mg-PO_{4}^{3-}$/1, 50 mg-hexadecane/1. The mineralization rate was found to be significantly affected by the temperature and the $Q_{10}$ value was 2.2. Teh optimal salinity of the strain ND601P-2 was 2o/oo. The increased salinity caused the elevation of % respiration value and the prolonged lag phase.

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Isolation of Microorganisms for Petroleum Desulfurization and Evaluation of Its Desulfurization Activity for Diesel Oil (석유 탈황용 미생물 분리 및 디젤유에 대한 탈황능 평가)

  • Sohn, Ho-Yong;Chang, Je Hwan;Chang, Yong Keun;Chang, Ho Nam;Ryu, Hee Wook;Cho, Keoung Sook
    • Clean Technology
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    • v.2 no.1
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    • pp.53-59
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    • 1996
  • For the development of biocatalysts and processes for microbial desulfurization of petroleum, more than 60 microbial strains capable of DBT(Dibenzothiophene) degradation were isolated from oil-polluted soils through 3 months of continuous and enrichment cultures. Among them, A23-3 strain could grow on DBT as the only sulfur source, while hexadecane was not utilized as a carbon source. The rate of desulfurization by A23-3 in a DBT-glucose medium was satisfactory. The addition of yeast extract or trace metal solution accelerated the rate of desulfurization about 4.5~6.5 times. In case of actual diesel oil treatment, the specific rate of DBT degradation was $0.045g-DBT\;per\;g-cell{\cdot}hour$. A number of aromatic compounds heavier than $C_{14}$ in diesel oil were also degraded by A23-3. A23-3 strain was evaluated as a good catalyst for the production of low-sulfur, low-aromatic clean diesel oil.

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