• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.535-540
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• 1990

Bacitlus sphaericus ts-Dl290 was characterized comparatively with the wild type strain 1593 by themeasurements of the biosynthesis of total DNA, RNA and protein on the temperature-shift culturesat permissive temperature of $30^{\circ}C$ and at nonpermissive temperature of $42^{\circ}C$. The growth patterns of the wild type strain and ts-Dl290 were similar at $30^{\circ}C$, but at 4Z C the mutant almost did not grow (temperature-sensitivity). When the growth temperatures of both stains were shifted-up from $30^{\circ}C$ to $42^{\circ}C$ after a 4 hour culture, their growths were normal, but when shifted-down from $42^{\circ}C$ to $30^{\circ}C$ after a 4 h culture, the mutant did not grow. When shifted up from $30^{\circ}C$ to $42^{\circ}C$ after a 4 hculture, the DNA syntheses of the two strains were at a normal rate for 1 h, but after 1 h the biosynthesesdecreased. The rate of DNA synthesis of the wild type strain at the nonpermissive temperature was about 93%, and that of the mutant was about 50% of the ratio of the wild type strain, and the RNA synthesis of the wild type strain was maintained for 3 h, and that of the mutant for 2 h. Thereafter the RNA synthesis decreased, and the synthesis of proteins in the both strains were similarlykept high for 8 h. The reversibility of the DNA synthesis of the mutant at $42^{\circ}C$ was lessened whenthe culture times were increased.re times were increased.

• Journal of Life Science
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• v.18 no.2
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• pp.206-212
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• 2008

The quorum sensing (QS) regulatory network has been the subject of extensive studies during recent years and has also attracted a lot of attention because it both positively and negatively regulates various putative virulence factors, although initially considered to be a specialized system of Vibrio fischeri and related species. In this study, to identify the novel materials which inhibit QS system of microorganisms, extracts of eighteen natural products were tested by bioassay using N-(3-oxohexanoyl)-$_L$-homoserine lactone and N-(3-oxooctanoyl)-$_L$-homoserine lactone synthesized in this experiment and an Agrobacterium tumefaciens NT1 biosensor strain containing a traI::lacZ fusion. The result indicated that the extracts of cabbage, leek, and onion exhibited the QS inhibition activity. Thus, materials contained in the extracts were isolated via recycling preparative HPLC and were purified via a JAIGEL-LS255 column. The common fraction corresponding to a peak of the 83 min point of them quenched the quorum sensing of A. tumefaciens NT1 biosensor strain in ABMM containing X-gal and was designated quorum sensing inhibitor-83 min (QSI-83). The QSI-83 exhibited the heat stability and did not inhibit the growth of A. tumefaciens NTl. Furthermore, thin layer chromatography (TLC) results suggested that these novel materials may be antagonists of N-acyl homoserine lactone or may inhibit the QS autoinducer synthesis by Pseudomonas syringae pv. tabaci.

• Applied Biological Chemistry
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• v.27 no.2
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• pp.119-128
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• 1984

The unsaponifiable from rice bran oil was fractionated into 4-desmethyl-'4-monomethyl- and 4, 4-dimethylsterol (triterpene alcohol) fraction by thin layer chromatography (TLC), and sterol composition of the each fraction was analyzed by gas liquid chromatography(GLC). The sterol peaks not well separated by GLC were further fractionated by $AgNO_3-TLC$, then analyzed using GLC. Each components in the three sterol fractions were identified by GLC and gas chromatography-maps spectrometry. As the results, ten sterols were confirmed as 4-desmethylsterol, nine as 4-monomethylsterol and four as 4, 4-dimethylsterol. Such uncommon phytosterols in higher plants as fucosterol, 24-ethyllophenol, 4${\alpha}$-methylstigmasta-7, 25-dienol and 28-isocitrostadienol were detected in rice bran oil and the biosynthetic pathways of the phytosterols were deduced with all the identified sterols.

• Journal of the korean academy of Pediatric Dentistry
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• v.34 no.3
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• pp.398-407
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• 2007

This study was performed to evaluate possibility to reduce treatment time for child patient who have limited control activity during flowable composite resin restoration according to comparing microleakage and shear bond strength. Group I, II with Single $Bond^{TM}$, group III, IV with AQ Bond $Plus^{TM}$, group V, VI with Prompt L-$Pop^{TM}$, and group VII as control group without adhesive system. 12 premolars and 10 molars were assigned into each group. Restorative material was used the Filtek $Z350^{TM}$ flowable. The results were as follows; 1. The result of microleakage evaluation, in the group I, II with Single $Bond^{TM}$ showed low dye penetration score. Dye penetration between group I and group II were similar. 2. Results for shear bond strength at group I and group II showed values higher than other groups. (p<0.05) There were no statistical differences between group I and group II. (p>0.05) 3. There was no statistical differences among I and II, III and IV, V and VI groups. 4. Group VII showed significantly low shear bond strength than group I, II, V, VI(p<0.05). Group VII showed low value than group with III and IV, but no statistical significances. In conclusion, adhesive bonding resin was not affect significantly to microleakage and shear bond strength of flowable resin restoration. Therefore, to reduce the operation time, adhesive bonding resin and flowable composite resin can be cured at once. But contamination of saliva, location of cavities can affect to bond strength in clinical field.

• Korean Journal of Environmental Agriculture
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• v.32 no.3
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• pp.185-192
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• 2013

BACKGROUND: As a result of rural exodus, aging of rural population, development of high-yield rice varieties and so on, abandoned paddy fields of South Korea have generally increased. The phytosociological studies are needed to recommend the effective vegetation management methods of abandoned paddy fields. The current study performed an analysis of vegetation characteristics by classifying plant communities for abandoned paddy fields in South Korea. METHODS AND RESULTS: A comparative analysis of syntaxonomy, synecology, and syndynamics was performed by using the Z.-M. method of phytosociology and vegetation survey which was obtained from an enumeration district of total 203 phytosociological releves of 5 regions in Gangwon-do, 5 regions in Gyeonggi-do, 2 regions in Chungcheong-do, 18 regions in Gyeongsang-do, and 10 regions in Jeolla-do. Alopecurus aequalis community, Juncus effusus var. decipiens community and Salix koreensis community were, respectively, identified as successional plant communities of the early stage, the middle stage, and the late stage in the vegetation of abandoned paddy field. Sere of abandoned paddy field can be classified, developmentally, into annual plant stage, perennial plant stage, shrub plant stage, and forest tree stage. Several kinds of dominant species rather than a distinct species were found in the middle stage of abandoned paddy field. CONCLUSION(S): In this study, changing pattern in vegetational communities of abandoned paddy field were understood. These results will provide important information for restoring and conserving the ecology of fallow ground.

• Proceedings of the Korean Society of Toxicology Conference
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• 1998.10a
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• pp.36-36
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• 1998

Tumorigenicity of benzo(a)pyrene (BP) and benzo(a)pyrene diol epoxides ((+)BPDE-1, (-)BPDE-1) was investigated in transgenic TG-AC mice carrying v-Ha-ras oncogene fused to the promoter of the mouse embryonic a-like, z-globin gene. Animals were topically treated twice per week for 25weeks with BPDE (10$\mu$g/mouse) and BP (10, 20, 40$\mu$g/mouse). In addition, animals were treated with BPDE or BP (initiated) followed by TPA (2$\times$2.5$\mu$g/week, for 4 weeks) for promotion study. In the continuous treatment of BPDE or BP, animals treated with 40$\mu$g BP showed $100\%$ tumor response after 20 weeks, $40\%$ of mice for 20$\mu$g BP, and $20\%$ for (+)BPDE-1, but (-)BPDE-1 and 10$\mu$g BP did not show any tumor response. After 25 weeks, most tumors turned out to be carcinomas in animals treated with 40$\mu$g BP. In BPDE or BP/TPA Initiation-promotion study, papilloma response occurred earlier (6 weeks after TPA treatment) than in continuously treated animals with BPDE or BP. RT-PCR assay for transgene expression showed that BP or BPOE was not transgene dependent in its tumorigenicity, but TPA was. Several Cytokine genes(TGF-a, TNF-a) and c-myc gene expressions were monitored in skin tissues during BP carcinogenesis. In early stage of BP treatment, the gene expressions were elevated(c-myc,TGF-a) or unchanged(TNF-a) compared to control, but the levels were gradually decreased during both middle and late stages of cacinogenesis, Gene expression levels of skin papillomas in acetone initiated-TPA promoted animals were close to those of middle stage or between middle and late stages. i-NOS was also highly expressed in carcinoma and papilloma, These data suggest that transgene expressions of TG-AC mice were not dependent on BP carcinogenesis and that TG-AC mice were more sensitive to TPA regardless of types of initiators. In addition, genes(TGF-a, c-myc, TNF-a, i-NOS) were modulated in the skin during BP cacinogenesis or TPA promotion.

• Microbiology and Biotechnology Letters
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• v.7 no.3
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• pp.135-140
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• 1979

A Streptomyces sp. strain AS-727 which was capable of producing penicillinase, was isolated from soil. The enzyme production was affected by the carbon and nitrogen sources added. Among them so far tested, glucose (or maltose) and sodium nitrate increased the enzyme production. And the amount of enzyme prodced reached maximum in 4 days cultivation. The optimla pH and temperature of the penicillinase was between pH 6.0 to 8.0 and 4$0^{\circ}C$ respectively. The stabel pH range of the enzyme was stable at 4$0^{\circ}C$, but it lost about 30％ and 40％ of the the activity respectively when it was treated at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 60 minutes. The activity of the enzyme was inhibited by Z $n^{++}$, but A $g^{+}$, $Co^{++}$, $_Mn^{++}$, $Ca^{++}$, P $b^{++}$ did not affected enzyme activity. Peculiarly, the enzyme protein precipitated by freezing or addition of ammonium sulfate, urea, sodium chloride and some organic solvents as etanol, methanol, acetone was not dissolved in deionized water or any buffer solution.n.n.ion.n.n.

• Atmosphere
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• v.24 no.3
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• pp.433-444
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• 2014

Although the Radar-AWS Rainrate (RAR) calculation system operated by Korea Meteorological Administration estimated precipitation using 2-dimensional composite components of single polarization radars, this system has several limitations in estimating the precipitation accurately. To to overcome limitations of the RAR system, the Korea Meteorological Administration developed and operated the RMQ (Radar-based Multi-sensor Quantitative Precipitation Estimation) system, the improved version of NMQ (National Mosaic and Multi-sensor Quantitative Precipitation Estimation) system of NSSL (National Severe Storms Laboratory) for the Korean Peninsula. This study introduced the RMQ system domestically for the first time and verified the precipitation estimation performance of the RMQ system. The RMQ system consists of 4 main parts as the process of handling the single radar data, merging 3D reflectivity, QPE, and displaying result images. The first process (handling of the single radar data) has the pre-process of a radar data (transformation of data format and quality control), the production of a vertical profile of reflectivity and the correction of bright-band, and the conduction of hydrid scan reflectivity. The next process (merger of 3D reflectivity) produces the 3D composite reflectivity field after correcting the quality controlled single radar reflectivity. The QPE process classifies the precipitation types using multi-sensor information and estimates quantitative precipitation using several Z-R relationships which are proper for precipitation types. This process also corrects the precipitation using the AWS position with local gauge correction technique. The last process displays the final results transformed into images in the web-site. This study also estimated the accuracy of the RMQ system with five events in 2012 summer season and compared the results of the RAR (Radar-AWS Rainrate) and RMQ systems. The RMQ system ($2.36mm\;hr^{-1}$ in RMSE on average) is superior to the RAR system ($8.33mm\;hr^{-1}$ in RMSE) and improved by 73.25% in RMSE and 25.56% in correlation coefficient on average. The precipitation composite field images produced by the RMQ system are almost identical to the AWS (Automatic Weather Statioin) images. Therefore, the RMQ system has contributed to improve the accuracy of precipitation estimation using weather radars and operation of the RMQ system in the work field in future enables to cope with the extreme weather conditions actively.

• The Korean Journal of Pharmacology
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• v.18 no.1
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• pp.65-72
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• 1982

It has been known that retinoids are intrinsically of critical importance for control of premalignant epithelial cell differentiation. In the absence of retinoids, normal cellular differentiation and growth does not occur in epithelia such as those of trachea and bronchi. Furthermore, it was also reported that retinoid deficiency enhanced susceptibility to chemical carcinogenesis in the respiratory system, in the bladder, and in the colon of the experimental animal. In 1974, Bollag examined the effects of synthetic retinoids in prevention of development of cancer and demonstrated synthetic retinoids to have more favorable therapeutic index than retinoic acid for causing regression of skin papilloma in mice. Therefore, it was assumed that this anticarcinogenic effect of vitamin A derivatives could be due to modification of the metabolism of the carcinogenic polycyclic hydrocarbon, which must first be activated to exert their effect. Hill and Shih reported that vitamin A compounds and analogs had inhibitory effect on drug metabolizing enzyme from liver and lung tissue of mouse and hamster. Lucy suggested that the chemoprevention effect of vitamin A derivatives is due to reaction with molecular oxygen, and it is possible that inhibition of hydroxybenzpyrene formation is a result of this property. On the other hand, butylated hydroxytoluene which is a potent antioxidant strongly inhibited the formation of mammary tumor induced by dimethylbenranthracene. Also, it was observed that this antioxidant inhibited cancer induction in rats by N-2-fluo-renylacetamide. The purpose of this experiment was to investigate the effect of vitamin A derivatives such as retinoic acid and retinoid on drug-metabolizing enzyme and to determine whether riboflavin tetrabutylate or vitamin E could prevent of modify any changes induced by vitamin A delivatives in the rats. The results obtained were as followings. 1) Body weight was significantly reduced by retinoic acid, but not by retinoid. 2) Retinoic acid markedly increased liver weight while retincid showed no effect on liver weight. Treatment of riboflavin tetrabutylate did not affect retinoic acid-induced change in both body weight and liver weight. 3) Both retinoic acid and retinoid remarkably decreased the activity of aminopyrine demethylase. Pretreatment of riboflavin tetrabutylate, however, prevented inhibitory effect of retinoic acid on the enzyme activity. 4) No significant effect of vitamin E on aminopyrine demethylase was observed in both groups treated with retinoic acid and retinoid.

• Fisheries and Aquatic Sciences
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• v.22 no.12
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• pp.31.1-31.12
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• 2019

Background: The black-chinned tilapia, Sarotherodon melanotheron, is the most abundant fish species in the Nakwa (an open lagoon) and Brenu (a closed lagoon) in the Central Region of Ghana. Aspects of the life history characteristics and the ecology of the fish populations in both lagoons were studied to assess the bio-ecological status of this important resource. Methods: Fish samples were obtained from fishermen that fish on the Nakwa and Brenu lagoons using cast, drag and gill nets. The age of the fish was assessed from otoliths analysis and its growth modelled following the von Bertalanffy growth function. Morphometric characteristics of the fish populations were analysed using power regression and ANOVA for parameters comparisons, and Student's t test to determine whether species grew isometrically. The percentage occurrence method was used to analyse the stomach contents of the fish. Results: A total of 382 fish samples from both lagoons were measured, comprising 209 from Nakwa lagoon and 176 from Brenu lagoon. The size and weight of fish samples ranged between 3.9-11.5 cm total length and 1.0-27.3 g for Nakwa Lagoon and 5.6-12.8 cm total length and 3.2-29.8 g for the Brenu Lagoon. The estimated von Bertalanffy growth parameters were L∞ = 12.04 cm and K = 2.76/year for the Nakwa Lagoon samples and L∞ = 13.44 cm and K = 3.27/year for Brenu Lagoon samples. Daily otolith incremental rate ranged from 0.01-0.03 mm per day to 0.01-0.02 mm per day for Nakwa and Brenu lagoons, respectively. Stomach content analysis of the fish samples revealed that the species are planktivorous and the range of food varied between the lagoons. Green algae were the most prevalent food item in the stomachs of the fish samples from Nakwa with the frequency of 69% whilst diatoms (80.5%) were most prevalent phytoplanktonic food item for the fish in Brenu lagoon. Conclusions: The estimates of asymptotic length for the species in both lagoons are close to known values of the species length at first sexual maturity and points to intensive fishing pressure. As a consequence, a comprehensive sample-based survey is required in both lagoons to derive estimates of management reference points. The results of the stomach content analysis are beneficial to the construction of diet matrix for ecosystem models of the two systems.