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Chemical Compositions of Corni Fructus and Separating Properties of Its Flesh by drying (산수유 열매의 화학성분과 건조에 따른 과육분리특성)

  • Lee, Young-Chul;Kim, Young-Eon;Lee, Boo-Yong;Kim, Chul-Jin
    • Korean Journal of Food Science and Technology
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    • v.24 no.5
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    • pp.447-450
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    • 1992
  • An attempt was made to investigate the some constituents and separation of flesh by drying of Corni Fructus for utilization as processing foods of Cornus officianalis S. et Z. Moisture and reducing sugar contents of Corni Fructus flesh were 40.4% and 18.6%, respectively. The content of anthocyanin was 183.0 mg/100g(dry base). The free sugars of Corni Fructus were consisted of glucose and fructose and their contents were 7.9% and 9.0%, respectively. The compositions of organic acids were malic and succinic acids which contained 1.9% and 0.5%, respectively. The titrable acidity as malic acid and pH were 3.6% and 3.19. The decrease ratio of weight and the drying temperature as the optimum conditions for the separation of flesh and seed of Corni Fructus were $30{\sim}35%$ and $60^{\circ}C$.

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Antioxidant and Anti-diabetes Activity of Extracts from Machilus thunbergii S. et Z. (토후박 추출물의 항산화 및 항당뇨 활성)

  • Xu, Ming Lu;Hu, Jian He;Wang, Lan;Kim, Hyun-Sam;Jin, Cheng-Wu;Cho, Dong-Ha
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.1
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    • pp.34-39
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    • 2010
  • Machilus thunbergii was an important medicinal resource and distributed widely in China. In this study, the bioactivities of dichloromethane fraction (DF) and water fraction (AF) from Machilus thunbergii methanol extract were investigated. Total phenolic contents of DF and AF were 57.90 mg Gal/g and 189.92 mg Gal/g, and total flavonoid contents were 17.34 mg Que/g and 58.38 mg Que/g respectively. The $EC_{50}$ for DPPH radical scavenging activity of DF and AF were $24.37\;{\mu}g/m{\ell}$ and $2.10\;{\mu}g/m{\ell}$. Reducing power and hydroxyl radical scavenging activities of AF were higher than those of DF. The $\alpha$-glucosidase inhibitory activity of AF ($IC_{50}\;=\;1.13\;{\mu}g/m{\ell}$) was higher than that of DF ($IC_{50}\;=\;5.34\;{\mu}g/m{\ell}$). The cell viability was showed that only the DF had anti-proliferation effect on human cancer cell HT-29. These results suggested that both the DF and AF extract of Machilus thunbergii were potential materials for anti-diabetes and functional food for their radical scavenging activity.

Swarming Differentiation of Vibrio vulnificus Downregulates the Expression of the vvhBA Hemolysin Gene via the LuxS Quorum-Sensing System

  • Kim Moon-Young;Park Ra-Young;Choi Mi-Hwa;Sun Hui-Yu;Kim Choon-Mee;Kim Soo-Young;Rhee Joon-Haeng;Shin Sung-Heui
    • Journal of Microbiology
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    • v.44 no.2
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    • pp.226-232
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    • 2006
  • Swarming has proven to be a good in vitro model for bacterial surface adherence and colonization, and the swarming differentiation of a bacterium has been shown to be coupled with changes in the expression of virulence factors associated with its invasiveness, particularly in the early stages of infection. In this study, we attempted to determine whether the expression of vvhA, which encodes for hemolysin/cytolysin (VvhA), is either upregulated or downregulated during the swarming differentiation of V. vulnificus. The insertional inactivation of vvhA itself exerted no detectable effect on the expression of V. vulnificus swarming motility. However, in our lacZ-fused vvhA transcriptional reporter assay, vvhA expression decreased in swarming V. vulnificus as compared to non-swarming or planktonic V. vulnificus. The reduced expression of vvhA in swarming V. vulnificus increased as a result of the deletional inactivation of luxS, a gene associated with quorum sensing. These results show that vvhA expression in swarming V. vulnificus is downregulated via the activity of the LuxS quorum-sensing system, suggesting that VvhA performs no essential role in the invasiveness of V. vulnificus via the adherence to and colonization on the body surfaces required in the early stages of the infection. However, VvhA may playa significant role in the pathophysiological deterioration occurring after swarming V. vulnificus is differentiated into planktonic V. vulnificus.

The mediating effect of life stress on the relationship between paramedic student's health behavior and bioethics awareness (응급구조학과 학생의 건강행위와 생명윤리의식의 관계에서 생활스트레스의 매개효과)

  • Lee, Eun-Young;Kim, Jung Sun;Lee, Hyo-Cheol;Ko, Dae-Sik;Kang, Kwang-Soon;Kim, Chul-Tae
    • The Korean Journal of Emergency Medical Services
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    • v.23 no.3
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    • pp.97-110
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    • 2019
  • Purpose: This study aimed to investigate the role of life stress in the relation between health behaviors and bioethics awareness among paramedic students. Methods: This study recruited 238 paramedic students in F and R areas as participants. Multiple regression analysis was performed using Baron and Kenny's three-step procedure. Results: In the first stage, health behaviors had a significant effect on life stress (β=-.202, p<.01). In the second stage, health behaviors had a significant effect on bioethics awareness (β=.240, p<.001). In the third stage, life stress had a significant influence on bioethics awareness (β=-.167, p<.01). In the fourth stage, the regression coefficient of the third stage (β=.137) was less than that of the second stage (β=.159). In addition, when controlling for life stress, health behavior was shown to affect bioethics awareness (β=.206, p<.01). The mediating effect of life stress was found to be significant in the relation between health behavior and bioethics awareness (Z=2.04, p<.05). Conclusion: As health behavior is a factor that affects bioethics awareness through the mediation of living stress, universities should prepare training programs to improve the health behavior and reduce the life stress among students.

Characterization of Canthaxanthin Isomers Isolated from a New Soil Dietzia sp. and Their Antioxidant Activities

  • Venugopalan, Vijayalatha;Tripathi, Subhash K.;Nahar, Pradip;Saradhi, P. Pardha;Das, Rakha H.;Gautam, Hemant K.
    • Journal of Microbiology and Biotechnology
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    • v.23 no.2
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    • pp.237-245
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    • 2013
  • Canthaxanthin (cx) is a potent antioxidant that is chemically synthesized at the industrial scale and has imperative applications in the cosmetic and feed industries. An orange pigmented mesophilic bacterium, designated as K44, was isolated from soil samples of Kargil, India. Biochemical tests, 16S rRNA gene sequencing, and FAME analysis of the bacterium indicated it to belong in the genus Dietzia and is distinct from human isolates. The strain showed 98% 16S rRNA gene sequence homology with Dietzia maris DSM 43102. High-performance liquid chromatography profile of the pigments isolated from K44 showed two major peaks absorbing at 465.3 and 475 nm. The liquid chromatography-mass spectrometry (LC-MS) analysis of both these peaks revealed their m/z to be 564. The molecular weights, LC-MS/MS fragmentation patterns, and ${\lambda}_{max}$ of these fractions corresponded to all-trans- (475 nm) and 9-cis-(465.3 nm) cx isomers. The antioxidant activities of cis- and trans-cx isomers isolated from this bacterium were found to differ, where the cis-isomer showed higher free radical, superoxide radical, and reactive oxygen species scavenging activities than the alltrans- isomer, suggesting that 9-cis-cx is more effective as an antioxidant than the all-trans-cx.

A STUDY ON THE SOLUBILITY OF DENTAL RESTORATIVE MATERIALS (치과용 수복재의 용해성에 관한 분석연구)

  • Na, Keung-Kyun;Park, Sang-Jin
    • Restorative Dentistry and Endodontics
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    • v.16 no.1
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    • pp.87-105
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    • 1991
  • The purpose of this experiment was to measure the leaking and solubility of commonly used dental restorative materials - Silux plus (CS), Hi-pol (CH), Clearfil F-II, Fissureseal (FS), Glass-Ionomer cement Fuji Type II (GI), Amalgam Cavex 68 (AM), Zinc Phosphate Cement (ZP) and gutta-percha (GP) and investigate the relation between the solubility and marginal leakage. Disc-shape specimens were fabricated with each material and dipped into deionized water, 0.01M lactic acid and 0.005M KOH solution, thus the total ionic concentrations in each solution was measured with ion chromatograph after 1, 3, and 7 days, respectively. For the solubility test, each specimen was immersed in 0.001M and 0.01M lactic acid for 24 hours, respectively and total weight loss was calculated. Also, Zn leaking through the margin of restorations was measured. The obtained results were as follows: 1. The amounts of eluted ion from the eight materials were most in 0.01M lactic acid and least in deionized water. 2. Of the eight materials, the fluoride release was greatest for glass ionomer cement (GI) in 0.01 M lactic acid after 7 days. 3. In analysis of the divalent cation, Mg was eluted most for zinc phosphate cement (ZP) and Ca for Clearfil F-II (CF) in 0.01M lactic acid after 7 days. 4. In analysis of transition metals, Cu and Zn were detected only. 5. The solubility rate of eight materials was greater in 0.01M lactic acid than in 0.001M for 24 hours, for zinc phosphate cement (ZP) the rate was greatest (5.4%) in 0.001M lactic acid, and amalgam least (0.01%). 6. The Zn concentration of restorative material with Z.P.C base was greater in 0.01M lactic acid than in 0.001M lactic acid.

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Identification of Volatile Compounds of 4 Grape Species by Storage Conditions (전자코와 GC/MS를 이용한 포도 품종별 저장 조건에 따른 휘발성 향기 성분 연구)

  • Lee, Yun-Jeung;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.7
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    • pp.874-880
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    • 2007
  • Volatile flavor compounds of 4 grape species (Campbell, Sheridan, Red globe, and Meoru) were identified during 3-day storage at either $4^{\circ}C$ or room temperature. Each sample was analyzed by solid-phase micro-extraction (SPME) method combined with gas chromatography-mass spectrometry. Also electronic nose composed of 12 different metal oxide sensors was used to differentiate flavors of grapes. Sensitivities (delta $R_{gas}/R_{air}$) of sensors from electronic nose were obtained by principal component analysis (PCA). Proportion of the first principal component was 99.30% at $4^{\circ}C$ and 99.36% at room temperature, respectively. In our result, flavor patterns of grape can be differentiated according to the storage period. The major volatile flavor compounds were 1-hexanol, hexanoic acid and its ethyl ester, and phenylethyl alcohol with the presence of butanoic acid and its ethyl ester, acetic acid, benzeneacetic acid and its ethyl ester.

Relationship between Scene Movements and Cybersickness (화면 움직임과 Cybersickness의 관계에 관한 연구)

  • Park, Kyung-Soo;Choi, Jeong-A;Kim, Kyoung-Taek;Kim, Sang-Soo
    • Journal of the Ergonomics Society of Korea
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    • v.24 no.1
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    • pp.1-7
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    • 2005
  • This paper investigates the effects of scene movements on cybersickness to develop the guidelines of scene movements in virtual environments. The types of scene movements were made for both scene navigations(through the axes of X: lateral, Y: fore & after, and Z: vertical) and scene rotations(by pitch, roll and yaw). And there were each three levels of speed; 2.7, 4.5 and 6.3 /s(for navigation), and 10, 20 and 30 /s(for rotation) were conducted. Twelve participants were exposed to each scene for 15 minutes, and three tests were performed to measure the degree of sickness. Before and after subjects were exposed to virtual environments, they were requested to describe their sickness symptoms by means of answering the Simulator Sickness Questionnaire(SSQ). And the postural stability tests, in which the Center of Pressure(COP) of subjects were traced and recorded by a 'force platform', were conducted. During the exposure on virtual environments, the subjects were requested to rate the degree of nausea. For both navigation and rotation, the effects of speeds and axes were significant in the SSQ scores and the nausea ratings, while it was not in the COP. The correlation between the SSQ scores and the COP data was not found. Therefore, it was inappropriate to use COP as a measure of cybersickness. The degree of sickness increased, except for the case of the yaw, as the speed increased. The sickness was most severe in the scene navigation through the axis X and in the scene rotation by the yaw.

Research on the Production Method of Three-Dimensional Image Scanimation (3D 입체 이미지 스케니메이션 제작 기법 연구)

  • Shan, Xinyi;Chung, Jean-Hun
    • Journal of Digital Convergence
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    • v.14 no.12
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    • pp.209-215
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    • 2016
  • The quantity of image frames, the widths of transparent slits, and the black bars on the scanline are the three basic elements of scanimation. Besides, the size of scanimation, the color and contrast of scanimation, and the brightness of scanline, etc, can also influence the optical illusion of scanimation. Based on the recent principle of production of 2D scanimation, and through asking questions, and making corresponding experiment, this research finally gets to the conclusion. Based on the principle of production of 3D scanimation, and through various basic testing, this paper aims to verify how to bring out the best visual effects (optical illusion) of animated illusion scanimation in publications by using the 3D animation in the publications. And the future goal is to study and flexibly use Z-axis space in the scanimation.

Gaze Effects on Spatial and Kinematic Characteristics in Pointing to a Remembered Target

  • Ryu, Young-Uk;Kim, Won-Dae;Kim, Hyeong-Dong
    • Physical Therapy Korea
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    • v.13 no.4
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    • pp.23-29
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    • 2006
  • The purpose of the present study was to examine gaze effects on spatial and kinematic characteristics during a pointing task. Subjects were asked to watch and point to an aimed target (2 mm in diameter) displayed on a vertically mounted board. Four gaze conditions were developed as combinations of "seeing-aiming" in terms of the eye movements: Focal-Focal (F-F), Focal-Fixing (F-X), Fixing-Focal (X-F), and Fixing-Fixing (X-X). Both the home target and an aimed target were presented for 1 second and then were disappeared in F-F and X-F. In X-F and X-X, only an aimed target disappeared after 1 second. Subjects were asked to point (with index finger tip) to an aimed target accurately as soon as the aimed target was removed. A significant main effect of gaze was found (p<.01) for normalized movement time. Peripheral retina targets had significantly larger absolute error compared to central retina targets on the x (medio-lateral) and z (superior-inferior) axes (p<.01). A significant undershooting to peripheral retina targets on the x axis was found (p<.01). F-F and X-F had larger peak velocities compared to F-X and X-X (p<.01). F-F and X-F were characterized by more time spent in the deceleration phase compared to F-X and X-X (p<.01). The present study demonstrates that central vision utilizes a form of on-line visual processing to reach to an object, and thus increases spatial accuracy. However, peripheral vision utilizes a relatively off-line visual processing with a dependency on proprioceptive information.

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