• Journal of Food Hygiene and Safety
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• v.38 no.6
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• pp.449-456
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• 2023

In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 10⁰ CFU/mL to 10⁶ CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)-mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37℃. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 10⁶ CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.475-479
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• 2003

Glycosaminoglycans(GAGs) from sea squirt, Styela clava was extracted with sodium phosphate at $105^{\circ}C$ for 2 hr and deprotein with trichloroacetic acid or hydrochloride. This GAGs was mainly constituted of galactose, glucosamine, glucose, mannose and galactosamine, and was phenylalanine, threonine, glutamic acid and aspartic acid. Mineral contents was mainly constituted 3.0mg% sodium, 1.6mg% potassium and 1.2mg% phosphorus and heavy metal was not detected. At pharmaceutical and cosmetic code of GAGs, protein and sulfate contents should included each range $14.0{\sim}22.0%$, $35.0 {\sim}45.0%$. After 5.0% trichloroacetic acid(w/v) and 10.0% HCl(v/v) treatment, protein and sulfate contents of GAGs was contained each 35.1%, 35.4% and each 22.0%, 18.5%.

• Bulletin of the Korean Chemical Society
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• v.30 no.12
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• pp.2895-2898
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• 2009

The complete structural characterization of dehydrodivanillin, an important natural product of interest to the food, cosmetics and aroma industries, has been carried out using multi-dimensional NMR spectroscopic techniques, and its previously $reported^{13}$C-NMR values have been reassigned. Dense and granular thin films of dehydrodivanillin have been grown by sublimation under high vacuum and studied using Scanning Electron Microscopy (SEM), electrical and optical techniques. The transmittance spectra of the films indicate a wide optical band gap of more than 3 eV. Typical J-V characteristics of Glass/ITO/dehydrodivanillin/Al structure exhibited moderate current densities ${\sim}10^{-4}\;A/cm^2$ at voltages > 25 V with an appreciable SCLC mobility of the order of $10^{-6}\;cm^2$/V-s.

• Journal of Animal Environmental Science
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• v.16 no.3
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• pp.193-204
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• 2010

The goal of this study was to develop a high-rise hog building(HRHB) for growing-fattening stages. HRHB was two story building and was suitable for specific environment in Korea. Manure was treated in a first floor and pigs were raised on the slatted second floor. Three ventilation systems - 1) duct inlet to wall exhaust system(V1), 2) eave inlet to wall exhaust system(V2), and 3) ceiling inlet to wall exhaust system(V3) - were used. This experiment was conducted during winter and from summer to fall. Air temperature, air speed, ammonia, hydrogen sulfide in HRHB, and swine growth rate were measured. During winter, air temperature in V1 system tended to be slightly high without any effect of outside air temperature. Maximum temperature from summer to fall was between 33.4 and $33.8^{\circ}C$ and there was no significant difference among systems. Continuously measured daily temperature was lower in V2 system than other systems and the fluctuation of air temperature was high. Air speed in V1 and V2 systems were similar (0.02~0.21 m/s), and was 0.04~0.15 m/s in V3 during winter. From summer to fall, air speed in V1, V2, and V3 systems were 0.10~0.41 m/s, 0.10~0.83 m/s, and 0.11~0.26 m/s, respectively. V2 system showed bigger fluctuation of air speed than other systems. During winter, the highest concentrations of ammonia in V1, V2, and V3 systems were 7.0, 3.5, and 8.7 ppm, respectively. Hydrogen sulfide was not detected. The highest concentrations of ammonia from summer to winter in V1, V2, and V3 systems were 6.1, 2.8, and 5.6 ppm, respectively. Swine growth showed no statistical significance among systems. However, daily weight gain was approximately 4% higher in V1 and V3 than in V2. Feed intake/daily weight gain was approximately 4% higher in V1 than other systems. From summer to fall, daily weight gain in V1 and V3 tended to approximately 3% higher than other systems, and feed intake/daily weight gain was approximately 2% higher in V1 than other systems. Hence, V2 system for the ventilation system of HRHB should not be utilized.

• Nuclear Engineering and Technology
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• v.54 no.8
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• pp.2871-2876
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• 2022

The sensitivity of polymer-based capacitive relative humidity (RH) sensors after irradiation with neutrons, electrons and protons was measured. Degradation consists of the decreasing of the upper RH limit that can be measured. At the same time, low RH-level sensitivity is almost stable. After 30 krad of absorption dose, RH cut off is equal to 85% of max value, after 60 krad-40%. Degradation reduces after annealing which indicates high radiation sensitivity of the internal circuit in comparison to RH-sensing polymer film.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.687-698
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• 2010

Borehole drilled depend on the point is bound to be limited to obtain the 2-D or 3-D layer information for entire targer area. On the other hand, SASW and MASW provide the sectional form of layer information through the shear wave velocity($V_s$). Therefore the useful information of the target area can be derived from SASW, MASW and borehole data. In this research, the correlation reflected locality and nationwide between sectional geo-layer and $V_s$ was investigated and analyzed. The target areas are westside of Pyeongtaek and Incheon. The shear wave velocity($V_s$) obtained from SASW, MASW and borehole data conducted within the scope of crossline for survey was utilized in each region. In the 2D distribution of $V_s$ from SASW, MASW, $V_s$ tend to continually increase deeper and deeper. By the target area, the depth of each representative geo-layer was nested on the sectional distribution map of $V_s$ to suggest the range of $V_s$ in accordance of strata by using borehole data. The 2D sectional geo-layer distribution map is presented based on the range of $V_s$. In addition the correlation between measured and calculated $V_s$ according to the empirical equation was analyzed.

• Korean Journal of Microbiology
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• v.37 no.4
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• pp.312-316
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• 2001

To improve the ethanol fermentability, four Saccharomyces yeast strains with efficient ethanol fermentability were subjected to rare-mating and protoplast fusion. Using these 4 strains, 5 different combinations of mating-pair or fusion-pair were constructed and their hybrids or fusants were obtained. From the statistical analysis of the results of the ethanol fermentation by the hybrids of the different mating-pair or fusion-pair, no difference was found in ethanol production, but [S. kluveri $khl{\times}S$ cerevisiae cp3] pair was shown to be the best combination which can produce high cell-viability. In fact, the clone No. 3 of the [S. kluveri $khl{\times}S$ cerevisiae cp3] pair was selected as the best strain which produced ethanol of 10.11% (w/v) or 12.81% (v/v) from 25% (w/v) glucose at $33^{\circ}C$ for 3 days with the residual sugar of 3.53% (w/v), viability of 62.65%, fermentation efficiency of 92.2%.

• Korean Journal of Crystallography
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• v.12 no.4
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• pp.197-206
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• 2001

A stoichiometric mixture of evaporating materials for AgInS₂ single crystal thin films was prepared from horizontal electric furnace. To obtain the single crystal thin films. AgInS₂ mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy(HWE)system. The source and substrate temperatures were 680℃ and 410℃, respectively. The crystalline structure of the single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction(DCXD). The carrier density and mobility of AgInS₂ single crystal thin film mea-sured from Hall effect by van der Pauw method are 9.35×10/sup 16/㎤ and 294㎠/V·s at 293K respectively. The temperature dependence of the energy band gap of the AgInS₂ obtained from the absorption spectra was well described by the Varshni's relation , E/sub g/(T)=2.1365eV-(9.89×10/sup-3/eV/K/)T²(T+2930K). The crystal field and the spin-orbit splitting energies for the valence band of the AgInS₂ have been estimated to be 0.1541eV and 0.0129 eV, respectively, by means of the photocur-rent spectra and the Hopfield quasicubic model. These results indicate that the splitting of the Δso definitely exists in the Γ/sub 5/ states of the valence band of the AgInS₂ /GaAs epilayer. The three photo-current peaks ovserved at 10K are ascribed to the A₁-, B-₁and C₁-exction peaks for n=1.

• Journal of fish pathology
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• v.18 no.2
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• pp.133-146
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• 2005

A bacteriological survey in maricultured fish farms was conducted in Korea from 2002 to 2004. A total number of 166 Vibrio isolates were collected from diseased fishes, including olive flounder (133 isolates), black rock fish (8 isolates), red sea bream (6 isolates), shrimp (5 isolates), black sea bream (4 isolates), abalone (3 isolates) and other fishes (7 isolates). All isolates were phenotypically characterized and then groups were obtained using the traditional biochemical test. Representative isolates of each group were genotypically characterized with sequencing the 16S rRNA genes or 16S-23S intergenic space genes. Above 14 species of Vibrio were identified as V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) and others Vibrio sp. (24 strains) by combining of biochemial and genetic characteristics.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.357-360
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• 2013

A 1.8V 2-Gb/s scalable low voltage signaling (SLVS) transmitter (TX) is designed for mobile applications requiring high speed and low power consumption. It consists of 4-lane TX for data transmission, 1-lane TX for a source synchronous clocking, and a 8-phase clock generator. The proposed SLVS TX has the scaling voltage swing from 50 mV to 650 mV and supports a high speed (HS) mode and a low power (LP) mode. An output impedance calibration scheme for the SVLS TX is proposed to improve the signal integrity. The proposed SLVS TX is implemented by using a $0.18-{\mu}m$ 1-poly 6-metal CMOS with a 1.8V supply. The simulated data jitter of the implemented SLVS TX is about 8.04 ps at the data rate of 2-Gbps. The area and power consumption of the 1-lane of the proposed SLVS TX are $422{\times}474{\mu}m^2$ and 5.35 mW/Gb/s, respectively.