• 한국수정란이식학회지
• /
• 제14권3호
• /
• pp.185-193
• /
• 1999

To improve the efficiency of in vitro production of embryos with follicular oocytes in pig, the recovery rates, in vitro fertilization and development. The results obtained were as fellows: The number of oocytes recovered 37 ovary was 1,365 by aspiration, 1,884 by slicing and 3,830 aspiration post slicing, per ovary was averaged 103.5 aspiration post slicing than 30.7 by aspiration and 50.8 by slicing (P<0.05). The percentage of grade I and II oocytes recovered was 0.05∼0.2% and 1.7∼2.3% respectively(p<0.05). The fertilization rates of ejaculate and epididymis sperm was 83.0 and 83.1%. And cleavaged rate was 60.8 and 69.0% respectively(P<0.05). However, there were no significant differences between sperm sources. The clevage rates of fertilized oocyte was significantly(P<0.05) higher as B.O(92.8%) than TALP (90.1%) or mTBM (80.1%). And in vitro developed to blastocyst rates of mTBM media used for fertilization was significantly (P<0.05) higher as 12.4%, compared with the results using the media of TALP(1.6%) or B.O (0.0%). The embryos developed 2-cell stage after in vitro fertilization were co-cultured with or without POEC and BOEC in NCSU-23 and TCM-199 media. In vitro developed to blastocyst rates was NCSU-23 with POEC(2.3%) or BOEC(1.2%), but in vitro cultured in TCM-199 medium with POEC or BOEC was not developed to blastocyst. The percentage of embryos that developed to morula stage in 0, 50, 100, 200 and 250uM was 16.6, 22.0, 13.5, 19.0 and 22.0%, respectively.

• 한국식품저장유통학회지
• /
• 제8권1호
• /
• pp.92-101
• /
• 2001

전통 발효 식품인 가자미 식 해로부터 cholesterol oxidase 생산성이 있는 균주를 분리하고 이들 분리된 균주들로 부터 여러 단계의 균주 선별시험을 통하여 cholesterol oxidate 생산성이 우수한 미생물을 선별하여 그 특성을 조사하였다. Cholesterol oxidase의 생산성이 가장 우수한 SFF34의 배양학적, 생리학적 특성 및 세포벽 지방산 조성을 조사하여 Bergey's Mannual of Systematic Bacteriology의 분류기준에 따라 동정한 결과 Bacillus sp. 또는 그 유연균으로 동정되어 분리균을 Bacillus sp. SFF34로 명명하였다. Cholesterol oxidase의 생산 조건을 검토한 결과 최적 배지 조성은 2.0% glucose, 0.5% yeast extract, 0.03% MgSO$_4$.7$H_2O$, 0.02% $K_2$HPO$_4$, 0.2% NH$_4$NO$_3$, 0.2% Cholesterol로 판명되었으며, 배양 조건은 3$0^{\circ}C$, 초기 pH 7.0, 진탕속도는 150rpm에서 24시간 배양시효소 생성이 가장 우수하였다 (2.42 U/ml).

• 한국식품영양과학회지
• /
• 제17권4호
• /
• pp.348-357
• /
• 1988

갈변반응에 관여하는 polyphenol oxidase(PPO : EC 1.10.3.1)를 한국산 고구마(Ipomoea batatas, val : Hong-mi)로부터 추출하여 ammoniun sulfate 분획 및 DEAE-cellulose column chromatography법에 의하여 정제한 결과, 효소활동도는 23.1배였으며 enzyme activity 수율은 41.5%이었다. 이 효소는 일반 전기 영동법에 의하여 8개의 isozymes 으로, 또한 isolectric focusing에 의하여 pI가 각각 다른 12개의 isozymes으로 분리되었고 그 pI의 범위는 3.2-9.6이었으며, Isoelectric focusing에 의하여 분리된 각 isozyme의 specific activity는 6,000-46,700U/mg protein의 범위에 있었다. 고구마 중의 PPO는 $65^{\circ}C$이하에서는 안정하였으며 $65^{\circ}C$ 에서는 1분 가열에 의하여 약 50%의 효소활성이 상실되었고, pH optimum은 6.0-6.5이었다. o-diphenol이 이 효소의 가장 좋은 기질로서, 이 효소는 o-diphenolase임이 확인되었고, catechol에 대한 Km치는 6.7mM로 나타났다. 또한 이 효소에 대한 저해작용은 dithiothreitol, cysteine 및 ascorbic acid 순으로 크게 나타났다.

• 환경위생공학
• /
• 제12권1호
• /
• pp.15-23
• /
• 1997

The anionic exchange resins with the Dowex-1 and Amberlite CG-400 form were transformed into resin of sulfate and acetate acid form, respectively. The uranyl complex ions with SO$_{4}$$^{2-}$ and CH$_{3}$COO$^{-}$ were adsorbed on the anion exchange resion mentioned above, and these complex ions were eluted as mixture eluents of 0.7M HNO$_{3}$ - 0.5M NH$_{4}$NO$_{3}$ by anion exchange chromatography. The optimum adsorption conditions of uranyl anion complex ions adsorbed on the upper of the resin colmun were 1.5-2.0 ml/min of flow rates at pH 2.0 and adsorptive power of uranyl complex ion of sulfuric acid type were nearly consistent with the Caussion normal distribution curve, whereas the elution state of UO$_{2}$(Ac)$_{2}$$^{4-}$ with acetic acid type was departed. The weighing form obtained from resin of sulfuric acid and aceric acid type was U$_{3}$O$_{8}$ whose recovery was 91.7%. The possibility of recovering uranium from the monazite sulfate solution using a strong base anion resin, Amberlite CG-400(sulfate form), was successfully recovered more than 90%.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권11호
• /
• pp.1412-1420
• /
• 2010

In this study, changes in testicular tissues of rats subjected to xylene and formaldehyde inhalation were evaluated. Three experimental groups were included in the study. Each group of rats was exposed to formaldehyde (6 ppm), technical xylene (300 ppm) or a combination of these two agents (150 ppm+3 ppm) for 8 weeks (8 h/d). Control groups were maintained for a period of eight weeks under the same conditions. Staining methods (triple staining, strep ABC method) were applied to examine histometric changes and relaxin like factor (RLF) expression in the testicular tissue. Immunostaining for RLF showed that density of staining for RLF decreased in rats exposed to formaldehyde. Formaldehyde or a combination of formaldehyde and xylene led to a decrease in seminiferous epithelial height. In conclusion, exposure of rats to formaldehyde and xylene-formaldehyde combinations adversely affects Leydig cells (RLF) and seminiferous epithelium of testicular tissue.

• Bulletin of the Korean Chemical Society
• /
• 제24권4호
• /
• pp.413-420
• /
• 2003

A gas chromatography/mass spectrometric assay method was developed for the simultaneous determination of neutral and bacis twenty-five disruptors $(ED_S)$ in frog and fish. Afther homogenization and sonication of 5 g of sample, purification was achieves in one step with a solid phase extraction procedure using silica gelflorisl. Eluton was performed with 50mL of acetone : n-hexane (1 : 9) solution. The eluate was concentrated to approximately 10uL and dissolves with 100 uL of hexane and analyzed by GC-MS (SIM). The peaks had good chromatographic properties and the extraction of these compounds from sample also gave relatively high recoveries with small variatoins. Detection limits were 0.1 ng/g for 4-nitrotoluene, benzophenone, hexachlorobenzene, atrazine, malathion, o,p-DDT, o,p-DDT and permethrin, and 0.2 ng/g for heptachlor epoxide, γ-chlordane, α-chlordane, p,p'-DDE, p,p'-DDD, cypermethrin and fenvalerate, and 0.3 ng/g for trifluralin, metribuzin, alachlor, dieldrin and p,p'-DDT, and 0.5 ng/g for heptachlor, aldrin and parathion, and 0.7 ng/g for endrin, and 0.8 ng/g for nitrofen. The recoveries were between 33 and 109%. The method was used to analyze twenty-five frogs and forty-six fishes fishes samples caught from various regions in Korea. Benzophenone was detected at concentration of up to 17.2 ng/g in frog or fish. Heptachlor, aldrin, γ-chlordane, p,p'-DDE, p,p'-DDD, endrin and o,p-DDD were detected at concentrations of 0.7-12.5 ng/g in frog or fish. Also significant leveles of dieldrin (up to 22.5 ng/g) were observed. The developed method may be valuable to be used to the national monitoring project of EDS in biota samples.

• 한국독어학회지:독어학
• /
• 제8집
• /
• pp.175-197
• /
• 2003

Die vorligende Arbeit behandelt die Probleme der $Koh\"{a}renz$ und Interpretation von van de Velde unter einigen Gesichtspunkten, welche mit der Frage $zusammenh\"{a}ngen$: Wie wird Sprachverstehen $erm\"{o}glicht$? Nach van de Velde besteht der wissen­schaftliche Zugang zu den Sprachverstehensprozessen und Interpretationsstrategien darin, dass man Erkenntnisse $\"{u}ber$ ihre bedingenden Faktoren gewinnt Seine Arbeit versucht diese bedingenden Faktoren in folgenden Gegebenheiten aufzufinden: 1) in den innersprachlichen Gegebenheiten, 2) in den $Referenzzusammenh\"{a}ngen$, auf die sich innersprachliche Gegebenheiten beziehen, 3) in den Handlungskontexten, in denen die verwendeten Sprachgegebenheiten ihre Funktionen haben, 4) in den zwischenmenschlichen $Kormnunikationsrahmenverh\"{a}lnissen$, 5) in den kognitiven Prozessen des Sprachrezipienten, in der neuro(psycho)logischen Ausstattung des Sprachbenutzers. Genau gesagt, bleiben die folgenden Fragen trotzdem immer nur offen: 1) Kann jede Ebene $f\"{u}r$ die $Koh\"{a}renz$ bei van de Velde in der Wirklichkeit schon da sein? 2) Sind die Wertigkeiten jeder Ebenen gleich? 3) Muss die $Koh\"{a}renz$ zwischen Inferenz und Verstehen sein? 4) Gibt es irgendeine Einheitstheorie, solche $interdisziplin\"{a}re$ Faktoren zu beherrschen?

• 한국세라믹학회지
• /
• 제40권8호
• /
• pp.804-810
• /
• 2003

건설폐기물 중 폐콘크리트를 40$0^{\circ}C$로 가열한 후 ball mill로 분쇄하여 재생골재를 생산할 때 발생된 미분말을 보통 포틀랜드 시멘트의 SiO$_2$, CaO, A1$_2$O$_3$원에 대한 대체원료물질로서 활용하고자 하였다. 클링커를 제조하기 위한 주원료는 석회석, shale, 전로슬래그, fly ash를 이용하였고, modulus는 LSF:91.0, SM:2.60, IM:1.60로 고정한 후 shale에 대해 미분말을 25, 50, 15, 100%까지 치환하여 클링커를 제조하였다. 클링커의 주요생성광물은 1,45$0^{\circ}C$에서 OPC와 같은 광물상인 $C_3$S, $\beta$-C$_2$S, $C_3$A, $C_4$AF 상으로 나타났다. 조합원료의 반응성을 비교하기 위해 시차열분석을 실시하고, 소성성지수를 구한 결과, 클링커의 광물상 생성온도는 plain과 유사했으며, 소성성지수는 48.6-51.4 정도로 나타났다.

• 대한약리학회지
• /
• 제24권2호
• /
• pp.221-232
• /
• 1988

본 연구는 스트렙토조토신 투여에의해 유도된 인슐린 의존적 당뇨 흰쥐에서 관찰되는 신혈류량의 감소가 내인성 혈관 수축계의 하나인 쓰롬복산의 영향이 증가된 때문인지를 규명하고자 하였다. 펜토바비탈을 이용하여 마취를 유도한 후 주어진 시간동안 아무런 약리학적 자극이 없는 상태에서 신동맥의 헐류량 (ml $min^{-1}$ $gKw^{-1})$, 혈압 (mm Hg) 및 그때의 신동맥 저항 (혈압/신동맥의 혈류량)을 관찰하였다. 그 각각에 대응하는 값은 당뇨쥐에서는 $5.9{\pm}0.3$(P<0.01, 대조군과 비교), $115{\pm}3$ 및 $20.3{\pm}1.0$(P<0.01, 대조군과 비교)이었고, 연령 대조군에서는 각각 $8.4{\pm}0.4$, $123{\pm}3$및 $15.1{\pm}0.8$이었다. 스트렙토조토신에 의한 고혈당 유도를 방지한다고 알려진 3-O-메칠 글루코즈로 진처리한 후에 스트렙토조토신을 투여한 흰쥐에서 관찰되는 혈압 및 신동맥의 혈류량은 연령 대조군의 값과 동일하였다. 신동맥이 확장될 수 있음이 확인된 상태에서, 쓰롬복산의 합성을 저해할 수 있는 용량의 OKY-1581(1 mg/kg, i.v.에 뒤이은 0.4mg/kg min 지속적 투여)는 대조군 (n=4)뿐 아니라 실험군(n=4) 흰쥐의 혈압, 신혈류량 및 신동맥의 저항을 변화시키지 많았다. 마찬가지로 쓰롬복산/프로스타글란딘 엔도펄옥사이드 효현제인 U46619에 의한 신동맥 수축을 저해할 수 있는 용량의 쓰롬복산 수용체에대한 길항제인 SQ29548(100 ng/m1 신혈류량)을 신동맥으로 투여했을 때에도, 관찰되는 신혈류역학에 아무런 변화가 없었다(n=8 각 군). OKY-1581 투여후에 사이클로옥시게나아제의 활성을 저해하는 약물인 인도메타신(2 mg/kg)을투여했을 때에도 관찰되는 신동맥의 저항은 대조군에서 뿐만 아니라 실험군인 당뇨쥐에서도 변화가 없었다. 따라서 본 연구 결과는 스트렙토조토신 투여후 관찰되는 신동맥의 저항 증가는 약물의 신장에대한 직접적인 독성이 아닌, 유도된 당뇨에 기인함을 제시하였고, 이러한 신동맥 저항 증가는 고조된 내인성 혈관 수축계의 하나인 쓰롬복산의 영향이 아님을 시사하고 있다.

• 대한기계학회논문집B
• /
• 제24권12호
• /
• pp.1582-1582
• /
• 2000

Rayleigh scattering and laser induced predissociative fluorescence are employed for capturing two-dimensional images of temperature and species concentration in a laminar nonpremixed flame of a diluted hydrogen jet. Rayleigh scattering cross-sections are experimentally obtained at 248nm. Dispersed LIPF spectra of OH and O₂ are also measured in a flame in order to confirm the excitation of single vibronic state of OH and O₂are excited on the P₁(8) line of the A ²∑+ (v＇=3) - X ²∏(v˝=0) band and R(17) line of the Schumann-Runge band B ³∑u- (v＇=0) - X ³∑g-∏(v˝=6), respectively. Fluorescence spectra of OH and Hot O₂ are captured and two-dimensional images of the hydrogen flame field are successfully visualized.