• Journal of Preventive Medicine and Public Health
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• v.31 no.4 s.63
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• pp.680-691
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• 1998

This study was conducted to examine the species differences in the urinary excretion of trichloroethanol(TCE-OH) and trichloroacetic acid(TCA) of trichloroethylene (TCE) metabolites and the effect of ethanol on these metabolites in mice and rats. TCE administered to Male Sprague Dawley rats and ICR mice as a single oral dose(100, 200, 500, 1,000 or 2,000 mg/kg body weight) and ethanol(3.0 g/kg body weight) was taken orally 12 hours before TCE administration. The metabolites in urine were measured 0, 12, 24, 36 and 48 hours after TCE administration. The results of metabolite excretion were as follows; Total trichlorocompounds(TTC) in urine increased with TCE dose in mice while increased only below dose of 1,000 mg/kg TCE in rats. The net excretion of TCE metabolites was significantly greater in mice than rats, although the proportion of TCE-OH to TCA was not different between mice and rats. These findings indicate that mice were internally exposed to significantly higher concentration of TCE metabolites than rats and this trend appeared to be more prominent with the increase of TCE dose. Ethanol increased significantly TCE-OH in urine of rats while the increase of TCE-OH induced by ethanol was not significant in mice, and didn't increase TCA of urine in both of rats and mice. This result suggests that the effect of ethanol on TCE metabolism may be due to the increase of TCE-OH.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.1
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• pp.51-58
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• 2014

Freezing hardiness of winter bud and branch of several pear (Pyrus pyrifolia) cultivars according to degree and duration of low temperatures was investigated by sprouting, electrolyte leaching rate and triphenyltetrazolium chloride (TTC). Sprouting rate as infected by degree and duration of low temperature were different between cultivars. The lower temperature, the longer duration, sprouting rate was decreased. Electrolyte leaching rate was showed above 30% at below $-30^{\circ}C$ treatment regardless of cultivars and duration. The lower temperature and the longer duration, Electrolyte leaching rate was increased. Electrolyte leaching rates of Manpungbae, Niitaka and Chuwhangbae at $-30^{\circ}C$ for 9 hours treatment which were observed high sprouting rate, were lower than those of other varieties. Absorbance rates by TTC test at $-21^{\circ}C$ treatment were 66.0 to 96.5% for 6 hours, 49.4 to 91.9% for 9 hours, and 37.3 to 89.4% for 12 hours. Freezing hardiness of pear cultivars at ecodormancy was different according to degree and duration of low temperature treatments.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.163-168
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• 2012

An optimization in vitro seed germination was established by using triphenol tetrazolium chloride (TTC) test, which has been known as two rare orchids (Gastrodia verrucosa Blume and Hetaeria sikokiana Tuyama) in Jeju island. We have established proper NaOCl treatment for in vitro germination of G. verrucosa and H. sikokiana through TTC test. In the case of H. sikokiana, seed viability through TTC test was high with 95% in control. However, NaOCl 1% treatment for 30 minutes showed the highest embryo swelling rate to seed viability. Likewise, swelling formation of embryos, diameter of embryos, protocorm formation and diameter of protocorms of G. verrucosa was 87%, $59{\mu}m$, 91% and $138{\mu}m$ through NaOCl 1% treatment for 30 minutes. This result will be applied on the basic information for improving in vitro seed germination rate of G. verrucosa and H. sikokiana.

• Information and Communications Magazine
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• v.13 no.12
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• pp.49-62
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• 1996

• Proceedings of the KSMRM Conference
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• 2002.11a
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• pp.114-114
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• 2002

Purpose： To know the fate of Gadophrin-2-enhanced areas in hearts with acute reperfused myocardial infarction. Method： The left anterior descending branches of coronary arteries were occluded for 90 min and reperfused for 60 min in 15 rabbits. Then, Gadophrin-2 (0.05 mmol/kg) was injected via ear veins. Short-axial T1-weighted spin echo images and fast cine images were obtained 24 hours after injection of Gadophrin-2. After four weeks, short-axial cine MRI was performed and T1-weighted spin echo Images were obtained 5 min and 15 min after injection of Gd-DTPA (0.2 mmol/kg). The animals were sacrificed and short-axial slices of the specimen were stained with 1.5% triphenyltetrazolium chloride (TTC) solution.

• Korean Journal of Veterinary Service
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• v.18 no.2
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• pp.163-176
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• 1995

Triphenyl Tetrazolium Chloride(TCC) reduction test is simple and sensitive to some residual antibiotics (especially to penicillin) in milk, but comparatively insensible to sulfo-namides. The volumn of sample is also large. Thus this study was undertaken to increase the detectable level of sulfonamides in raw milk. In this study, we used small transparent plastic hole and pulp disc instead of 10m1 test tube and made test medium in which was added 0.08%TTC, 0.3% agar, 10% skim milk, approximately $10^6$ CFU/ml streptococcus thermophilus and 5ppm Trimethoprim to enhance the sensitivity for sulfonamides The results of TCC reduction test by disc plate method were summarized as follows : 1. sensitivity to residual sulfonamides were much higher than official TCC reduction test. Detectable limites of sulfamethazine, sulfamerazine, sulfathiazole, sulfachloropy-ridazine, sulfadimethoxine, sulfamononethoxine, sulfadiazine and sulfaquinoxaline were 0.1-0.5ppm levels. 2. Detectable limites to some antibiotics were simillar or good than that of official method as 0.005-0.1ppm to three ${\beta}$ -lactams, 0.25-0.5ppm to one macrolide, 2-10ppm to three aminoglycosides, 0.2-0.5ppm to three tetracycline, 0.1-0.5ppm to chloramphenicol. 3. Only 0.1ml of milk was needed to test and the test medium could be stored appnoximatly 7days in the refrigerator. So test procedure was convenient than offcial method. 4. These results suggest that disc plate method is more useful to detect bacterial growth inhibition substances including sulfonamides in raw milk.

• Journal of Environmental Health Sciences
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• v.15 no.2
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• pp.41-49
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• 1989

The assay on microbial dehydrogenase activity by using TTC was investigated. The experimental results are summarized as follows: 1. The TF production was affected by dissolved oxygen. Dissolved oxygen in samples could be effectively removed by adding $Na_{2}SO_{3}$ as reductant prior to incubation. 2. The toxic effect on TF formation was observed at up to 0.1% of final TTC concentration when VSS concentration was 2.7g/l. As the VSS concentration increased the effect decreased. 3. It was clearly revealed that the TF generation rate decreased with increasing VSS concentration. The dilution of sludge could be applicable when VSS level is below 3.5g/l. 4. The TF production of endogenous phase (De) could be used as a measure for the viable fraction of sludge over various sludge ages. The additional TF production (Ds) due to extracellular substrate responded to De, not to VSS concentration.

• Korean Journal of Radiology
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• v.2 no.1
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• pp.21-27
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• 2001

Objective: To identify and evaluate the lateral border zone by comparing the size and distribution of the abnormal signal area demonstrated by MR imaging with the infarct area revealed by pathological examination in a reperfused myocardial infarction cat model. Materials and Methods: In eight cats, the left anterior descending coronary artery was occluded for 90 minutes, and this was followed by 90 minutes of reperfusion. ECG-triggered breath-hold turbo spin-echo T2-weighted MR images were initially obtained along the short axis of the heart before the administration of contrast media. After the injection of Gadomer-17 and Gadophrin-2, contrast-enhanced T1-weighted MR images were obtained for three hours. The size of the abnormal signal area seen on each image was compared with that of the infarct area after TTC staining. To assess ultrastructural changes in the myocardium at the infarct area, lateral border zone and normal myocardium, electron microscopic examination was performed. Results: The high signal area seen on T2-weighted images and the enhanced area seen on Gadomer-17-enhanced T1WI were larger than the enhanced area on Gadophrin-2-enhanced T1WI and the infarct area revealed by TTC staining; the difference was expressed as a percentage of the size of the total left ventricle mass (T2= 39.2 %; Gadomer-17 =37.25 % vs Gadophrin-2 = 29.6 %; TTC staining = 28.2 %; p < 0.05). The ultrastructural changes seen at the lateral border zone were compatible with reversible myocardial damage. Conclusion: In a reperfused myocardial infarction cat model, the presence and size of the lateral border zone can be determined by means of Gadomer-17- and Gadophrin-2-enhanced MR imaging.

• The Journal of the Korea Contents Association
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• v.19 no.7
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• pp.186-191
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• 2019

Introduction: In aged people, stroke incidence is increased. But standardized experimental animal protocol study for the research of stroke therapy is rare. There is little report on the success rate of cerebral artery occlusion model using standardized Nylon thread length of precise thread end-size controlled. Method: In this study, the operator intended the occlusion of middle cerebral artery (MCA) using $0.18{\pm}0.02mm$ end 5-0 Nylon thread. Middle cerebral artery occlusion was induced for 60min under isoflurane anesthesia. After 60min, the operator removed the Nylon thread and reperfusion was induced for 23hrs. The mice was killed 23hrs after reperfusion and infarction area of brain was confirmed by 1.5% TTC (2,3,5-tryphenyl tetrazolium chloride) staining. Results: According to end size and insert length of Nylon thread, Middle cerebral artery occlusion (n=50), internal carotid artery occlusion (n= 14), distal middle cerebral artery occlusion (n= 36), anterior cerebral artery (n= 1) were induced. And no infarction (n= 50) was observed. Conclusion: According to weight of mice, the operator induced reversible cerebral artery occlusion model by different insert length (30.0~36.9g : 9.0mm, 37.0~40.0g : 9.5mm) of Nylon thread. Success of cerebral artery occlusion model was confirmed by checking infarction area using TTC staining. The success rate (66.9%, 101/151) of reversible cerebral artery occlusion model in the mouse and the operational conditions are shown.

• Journal of Astronomy and Space Sciences
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• v.13 no.2
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• pp.30-40
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• 1996

The telecommand system of KITSAT micorsatellite receives commands from ground stations or on-board computers. It decodes, validates and delivers commands to sub-system. The telemetry system is to collect, process and format satellite housekeeping and mission data for use by on-board computer and ground station. It is crucial for the telemetry and telecommand system to have high reliability since the spacecraft operation is mostly based on the function of this system. The telemetry and telecommand(TTC) systems for KITSAT-1 and 2 had been developed under the consideratin of the space environment of Low-Earth-Orbit and the limited mass, volume and power of micorsatellite. Since both satellites were launched in August 1992 and September 1993 respectively, the have shown to be working successfully as well as the TTC systems on-board both satellites.