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조절되지 않은 실온에서의 돼지액상정액 보존에 관한 연구 (Study on the Preservation of Liquid Boar Semen at Uncontrolled Room Temperature)

  • 박창식;김민규;이성호;서직;이천군;이의해
    • 한국가축번식학회지
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    • 제21권1호
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    • pp.25-30
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    • 1997
  • This study was done to find out the preservation possibility of liquid boar semen at variabel room temperature of 9 to 16$^{\circ}C$. The percentages of sperm motility and NAR acrosome were highest in B tschwiler extender compared to B tschwiler+Hepes, Andro+Hepes and Andro extenders. The extenders with Hepes buffer showed detrimental effect for preservation of liquid boar semen. The pH of ejaculated sperm-rich fraction was 7.5. The pH of B tschwiler+Hepes, B tschwiler, Andro+Hepes and Andro extenders was 6.9, 7.5, 7.1 and 8.1, respectively. The pH of liquid boar semen with B tschwiler+Hepes, B tschwiler, Andro+Hepes and Andro extenders was 6.6, 6.9, 6.7 and 6.9 at 1st day of storage, and 5.5, 5.7, 5.6 and 5.8 at 7th day of storage, respectively. Gilts and sows were inseminated twice with liquid boar semen stored at 9~16$^{\circ}C$ in B tschwiler extender for 3~4 days. Farrowing rate, litter size and average pig weight at birth between AI and natural service did not differ significantly in gilt and sow, respectively. However, sow showed higher farrowing rate and litter size compared to gilt both in AI and in natural service. As a result of this study, we found out that liquid boar semen can be stored for 5~7 days at uncontrolled room temperature of 9~16$^{\circ}C$ in B tschwiler extender.

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질소시비수준이 Pioneer 988 ( Sorghum bicolor $\times$ S.Sudanesse ) 의 엽생육 및 건물생산에 미치는 영향 (Effect of N-levels on Growth and Dry Matter Accumlation of Sorghum-Sudangrass Hybrid ( Sorghum bicolor $\times$ S. sudanense ) , Pioneer 988)

  • 김상덕;윤익석
    • 한국초지조사료학회지
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    • 제4권2호
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    • pp.158-163
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    • 1983
  • 種實用 수수와 수단그라스의 交雜種인 Pioneer 988의, 窒素施肥水準에 따른 時期別 生育 및 乾物生産量을 조사하기 위하여 本 硏究를 실시하였다. 窒素肥科는 成分量으로 10a當 15, 25, 35kg을 施用하였으며 幼植物로부터 開花後期까지의 生育期間게 몇가지 特性을 調麥한바 그 結果는 다음과 같이 要約된다. 1. 出現葉數는 生育이 進行됨에 따라 증가했는데 穗孕期까지 급격한 증가를 보였고, 出穗期에는 最終葉인 15葉이 出現했다. 2. 葉面積指數는 生育의 進行에 따라 증가했는데, 특히 穗孕期까지 그 增加가 급격했으며, 室素施肥水準別로는 幼穗分化期까지 15kg 施肥區에서 컷으나 穗孕期 以後에는 35kg 施肥區에서 컷다. 3. 乾物收量은 穗孕期 以前에는 15kg 施肥區에서 많았으나, 穗孕期 以後에는 35kg 施肥區에서 많았다. 4 乾物生産速度는 純同化率 및 平均 葉面積指數와 밀접한 관계가 있었으며 生育後期에는 純同化率의 增加와 더욱 밀접한 관계를 나타냈다.

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고농도 칼륨처리를 활용한 원통형 종이포트 토마토묘의 도장억제 (Control of Stretching of Tomato (Lycopersicon esculentum Mill.) on Cylindrical Paper Pot Seedling Using High-Salinity Potassium Fertilizers)

  • 수찬;김시홍;김대훈;김재경;허재윤;넉탕부;최기영;김일섭;장동철
    • 생물환경조절학회지
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    • 제29권4호
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    • pp.354-364
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    • 2020
  • 본 연구는 원통형 종이포트를 활용한 토마토 육묘시, 염스트레스를 활용하여 고온기 도장 억제가능성을 검토하기 위하여 수행되었다. 시험구는 K2SO4, KCl과 KH2PO4을 각 5, 10 dS·m-1로 처리하였고, 또한, 토마토 모종에 고염도의 칼륨을 처리하여 수분 및 저온스트레스 환경에서의 적응성 및 생존성을 조사하였다. 조사결과, 처리 농도가 높아질수록 지상· 하부 건물중, 옆면적, 순동화율 (NAR)이 감소하고, 경경과 충실도는 증가하였다. 수분 스트레스 처리 이후, 대조구는 심한 위조현상을 보였지만, KCl처리구는 양호하였다. 상대수분함량은 대조구에서 23%, KCl처리구에서 8% 감소 하였다. 또한, 대조구에 비하여 KCl 처리구는 저장시(9, 12 및 15℃ 모종의 손상 비율이 낮았다. 이와 같은 결과로 보아, KCl과 같은 고농도의 칼륨 처리가 원통형 종이포트 토마토 육묘의 도장 억제에 효과적이며 환경 스트레스 내성을 향상시키는 것으로 판단된다.

Cucumber mosaic virus Paf 계통의 약독 병징과 관련된 satellite RNA의 유전자 해석 (Genomic Analysis of Satellite RNA of Cucumber mosaic virus-Paf Related with Mild Symptoms)

  • 성미영;정민영;이상용;류기현;최장경
    • 식물병연구
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    • 제10권4호
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    • pp.241-247
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    • 2004
  • Cucumber mosaic virus(CMV)-Paf 계통에 포함된 satellite RNA(Paf-satRNA)는 CMV의 병징을 완화시키는 약독병징 관련 유전자로 작용하였다(Choi 등, 2001). 이 연구는 Paf-satRNA의 약독병징 관련 유전자의 도메인을 확인하기 위하여, 고추에서 chlorosis 병징을 발현하는 PepY-satRNA와 키메라 satRNA를 구축하여 분석하였다. 두 종의 satRNA의 염기서열을 비교한 결과, 분자크기가 큰 PepY-satRNA에서 10염기의 삽입이 발견되기는 하였지만, 양 말단영역의 염기는 비교적 안정된 conserved sequence를 보였다. 그러나 이들 satRNA의 중간영역에 존재하는 염기서열, 즉 5' 말단의 81번째 염기로부터 113번째, 그리고 183번째 염기부터 265번째 염기까지의 영역에서는 많은 변화를 나타냈다. 약독병징과 관련된 도메인을 확인하기 위하여 구축한 각 satRNA 및 키메라 satRNA의 cDNA로부터 transcript RNA를 전사시키고, 전사된 각 satRNA transcript를 CMV-Fny의 게놈RNA1, RNA2 및 RNA3의 transcript와 혼합한 후 N. benthamiana에 접종하였다. 그 결과 RT-PCR에 의해서 모든 satRNA-cDNA로부터 전사된 transcript의 감염성이 확인되었으며, Paf-satRNA 및 키메라 Paf(H/N)-satRNA와 PepY(N/A)-satRNA를 접종한 N. benthamiana에서는 모두 약한 모자이크 또는 무병징 감염의 특성을 보였다. 이와는 대조적으로 PepY-satRNA 및 키메라 PepY(H/N)-satRNA와 Paf(N/A)-satRNA를 접종한 식물에서는 전형적인 모자이크 증상과 식물체의 위축을 동반하였다. 이들 각 키메라 satRNA에 감염된 N. benthamiana를 접종원으로 고추에 접종한 결과, Paf-satRNA와 혼합한 CMV-Fny를 접종한 고추에서는 무병징에 가까운 약한 모자이크 증상이 발현되었고, PepY-satRNA를 접종한 고추는 뚜렷한 chlorosis의 모자이크 증상이 발현되었다. 한편 이들 두 종 satRNA의 키메라, Paf(H/N)-satRNA와 PepY(N/A)-satRNA를 접종한 고추에서는 모두 약한 모자이크 또는 무병징 감염의 특성을 보였고, PepY(H/N)-satRNA와 Paf(N/A)-satRNA를 접종한 식물에서는 전형적인 chlorosis의 모자이크 증상과 식물체의 위축을 동반하였다. 이와 같은 결과를 종합해 보았을 때, N. benthamiana에서와 마찬가지로 Paf-satRNA의 약독병징과 관련된 유전자의 도메인은 HpaI-NarI 영역에 존재한다는 것을 나타냈다.

채식을 하는 중$\cdot$노년기 여성의 영양소 섭취 상태와 식사의 질 평가 (A Evaluation Study on Nutrient Intake Status and Diet Quality of Middle and Old Aged Vegetarian Women in Korea)

  • 김미현;배윤정;이다홍;조혜경;최선혜;승정자
    • 대한지역사회영양학회지
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    • 제10권6호
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    • pp.869-879
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    • 2005
  • The purpose of this study was to evaluate on nutrient intake status and diet quality of middle and old aged vegetarian women in Korea, The research group was composed of vegetarian women (n=91), all of them were Seven Day Adventists, who had been on vegetarian diets, over 20 yrs. Their anthropometric measurements, dietary intakes, and diet quality indices were compared to omnivores (n=122). The average age of vegetarians and omnivores were 61.8 yrs and 60.3 yrs, respectively. The mean daily energy intakes of vegetarians and omnivores were 1428.8 kcal and 1424.5 kcal, respectively and there was no significant difference. The vegetarians consumed significantly lower intakes of protein (p < 0.05), zinc (p < 0.001) and vitamin $B_{2}$ (p < 0.05) compared to omnivores. In the diet quality, zinc and vitamin $B_{2}$ nutrient adequacy ratio (NAR) of vegetarians were significantly lower than those of omnivores. Mean adequacy ratio (MAR) of vegetarians and omnivores were 0.80 and 0.77, respectively and there was no significant difference. The KDDS (Korean's Dietary Diversity Score) of vegetarians and omnivores were 3.7 and 4.0, respectively, and there was significant difference (p < 0.01). The KDQI (Korean's Diet Quality Index) of vegetarians (1.5) was significantly lower than that (2.1) of omnivores (p < 0.001). In conclusion, vegetarian women have low intake status of protein, zinc and vitamin $B_{2}$, and partly low diet quality. Therefore it was needed that well planed diets to replace the nutrients supplied from excluded food groups in middle and old aged vegetarian women. (Korean J Community Nutrition 10(6) : $869\∼879$, 2005)

Nutritional Status and Bone Mineral Density of Elderly Women in Asan

  • Kim Ji Sun;Kwon Young Suk;Shin Yoon Jeong;Kim Min Kyung;Kim Hee Seon
    • Journal of Community Nutrition
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    • 제7권1호
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    • pp.49-57
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    • 2005
  • Nutrition factors play an important role in the pathogenesis of osteoporosis. The purpose of this study is to investigate the relationship between nutritional status and bone mineral density of elderly women. Three hundred thirty five elderly women (over 65 years) in Asan were divided into three age groups (group 1, less than 70 y ; group 2, from 70 to 75 y ; group 3, 75 y or more). Total alkaline phosphatase and serum calcium (Ca) were analyzed using spectrophotometric procedure. Data for food and nutrient intakes were obtained by a 24-hour recall method. Bone density was measured by broadband ultrasound attenuation (BUA) using QUS-2. Age differences were tested with the X 2 test for categorical variables and with ANOVA and Tukey's test for continuous variables. Correlation was conducted to test the association between bone density and nutrient intake. The subjects in age groups 1,2 and 3 were $36.7\%$, $32.8\%$ and $30.4\%$, respectively. Height and body weight of the subjects were significantly decreased with age. Average bone density of the subjects in group 3 was lower than the other age groups. Osteoporosis determined by t-score is $17.9\%$ for group 1, $24.5\%$ for group 2 and $55.9\%$ for group 3 (p < 0.001). The serum Ca level of the subjects in group 2 was significantly lower than that of group 1 although mean values in all age groups are within the normal range. Dietary Ca intake, nutrient adequacy ratio (NAR) and index of nutrient quality (INQ) were decreased with age. Bone density was negatively correlated with age (p < 0.001), while body mass index (p < 0.01) was positively related with bone density. Although partial correlation did not reveal the significant correlation of BMD and dietary calcium after controlling for age, since calcium intake was very poor compared to sodium and phosphorous intakes, recommendation of more calcium intake for elderly women especially those over 75 years must be continuously emphasized.

Dietary Variety and Nutrient Intake by 24-hour Recall in Korean College Students

  • Seunghee Kye;Lee, Haeng-Shin;Kim, Bok-Hee;Whachun Yoo;Sung, Mi-Kyung;Lee, Hyun-Ja
    • Nutritional Sciences
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    • 제7권2호
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    • pp.92-97
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    • 2004
  • Despite the universal recommendation to eat a variety of foods, we still do not know whether and to what extent the variety affects dietary quality. This study was performed to evaluate the dietary variety scores as tools for assessing the dietary quality of Korean young adults. The 1-day dietary intake data were collected from 144 male and 214 female college students (>18 years) using the 24-hour recall method Relative nutrient intake compared to Korean Recommended Dietary Allowance (KRDA) as the nutrient adequacy ratio (NAR), were computed Also, the mean adequacy ratio (MAR) was calculated. Dietary variety score (DVS) was determined by counting the number of food items consumed daily, and the dietary diversity score (DDS) by counting the number of food groups consumed daily. Results showed that DVS, DDS and MAR were significantly correlated to each other. The MAR score significantly increased as DDS increased in both men and women. When different DVS (20$\leqDVS\leq$ 30) was evaluated for its sensitivity, specificity, measured prevalence, true prevalence, and positive and negative predictive values towards MAR, DVS 21 was revealed to be optimal dietary variety score as a cutoff point to differentiate Korean young adults with or without an adequate and balanced diet. Nutrient intakes of subjects who had DVS<21 were significantly lower than those of subjects with DVS$\qeq$ 21. These results indicate that the dietary variety score appeared to be an effective tool for evaluating the adequacy of diet in Korean young adults.

장년기 남자의 에너지 과잉섭취와 주류 또는 육류구이 섭취량간의 관련성에 관한 연구 (Caloric Intake to Fat or Alcoholic Drink Intake in Middle-Aged Men Is Highly Co-related than Those in Young Men)

  • 박영숙;한재라;김순경;김창임
    • 한국식품영양과학회지
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    • 제33권4호
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    • pp.679-686
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    • 2004
  • In order to find the relationships of nutrient intakes and food consumptions to calorie intake for young and middle-aged men, a study was peformed using the data of Korean National HealthㆍNutrition Survey in 1998. Data from 659 young men (20 to 29 years) and 1,697 middle-aged men (30 to 49 years) were analysed for percent of recommended daily allowance (%RDA), index of nutritional quality (INQ) and nutrient adequacy ratio (NAR) in order to evaluate one's Quality and quantity. We found that energy and nutrient intakes were not significantly different between young and middle-aged groups. Their calorie and nutrient intakes were below their RDA. The most insufficiently consumed nutrient (less than 75% of RDA) was calcium followed by thiamin vitamin A, and calorie. The lacks of calorie, protein, iron, vitamin C, and niacin were more widely-spread in the twenties than the middle-aged. The mean nutrient adequacy ratio of the middle-aged was better than that of the young, even though some INQ were worse. Only for the subjects consumed sufficient energy, there were positive relations between grains, meats or alcoholic drinks to calorie level in middle-aged men but there were positive relations between broiled fish/shell or fats/oils to calorie level in young men. It seemed that both middle-aged men eating alcoholic drinks, broiled meat, and grains and young men eating cake/cookies, rice cake/potato cake, grains, and sugars could consume enough calorie with nutritional imbalance.

Comparison of Yield and Growth Characteristics of Korean High Yielding Cultivars and IRRI's New Plant Type Rice Line

  • Lee, Byun-Woo;Ha, Jong-Ryuk
    • 한국작물학회지
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    • 제44권1호
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    • pp.59-63
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    • 1999
  • Yield and growth characteristics were compared for five rice cultivars; a new Tongil-type, so called "super-rice", Dasanbyeo, an old Tongil-type Milyang 23, two japonicas Dongjinbyeo and Ilpumbyeo, and a new plant type (NPT)line IR65600-27-1-2. The objective of this stusy was to clarify the high yielding capacity of Dasanbyeo in terms of growth characteristics. The average grain yield (9 t/ha) of Dasanbyeo was higher than that of Milyang 23 by ca. 9% that of japonicas by 20 to 30%, and that of NPT line by ca. 100%. The higher grain yield of Dasanbyeo was attributable not only to the greater dry matter production but also to the higher harvest index (HI). Dasanbyeo showed the greatest dry matter at harvest owing not only to the rapid leaf expan-sion at early growth stage and the resulting high LAI through the entire growth stage but also to the high NAR despite the high LAI. The rapid leaf expansion of Dasanbyeo at early growth stage seemed to be related in part to the profuse tillering capacity. HI was 0.53 in Dasanbyeo, 0,51 in Milyang 23, 0.41 in japonicas, and 0.35 in NPT line. Dasanbyeo was indebted for its higher HI to the relatively high grain filling ratio in spite of a much greater sink size than the other cultivars. Dasan had a greater source to sink ratio during grain ripening as measured by LAD/spikelet and dry matter production/spikelet which showed positove correlations with the grain ripening ratio. New plant type (NPT) line showed the lowest grain yield owing to the small sink size and the low grain filling ratio which seemed to have resulted from the abundant occurrence of weak-strength spikelets. The weak sink strength, in turn, seemed to have suppressed photosynthesis during the grain ripening stage.

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MMPI 척도로 본 한의대생의 형제자매관계와 성격특성의 연관성에 관한 연구 (A Study of the Correlation Between the Siblings Relation and the Personality Characteristics on the Oriental Medical University Students by MMPI Profiling)

  • 하지원;정진형;김보경
    • 동의신경정신과학회지
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    • 제23권4호
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    • pp.123-132
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    • 2012
  • Objectives : This study aims to investigate the correlation between the characteristics of personality on MMPI scale, and the siblings relation, number of current family living with, gender and age of the Oriental Medical University students. Methods : For this study, we carried out the MMPI profiling and gathered information regarding the sibling relation of the subjects. We studied the correlation between the two measures, and the correlation between MMPI scales and the subjects' gender and age. Results : 1. The group without siblings had higher STY figures then the groups with siblings. 2. There was no significant differences in MMPI profiles among the only child group, first-born group, second-born group, and third-born group. 3. The married group showed higher Pd, Pt, Sc, BDL figures than the unmarried group. 4. As the number of family members currently living with increased, the BDL and STY figures decreased. 5. The male group had higher Hs, Hy, Pa, Ma, NAR, BDL, ANT, CPS, PAR, and SZD figures, while the female group had higher D, Pd, Mf, Pt, HST, PAG, and DEP figures. 6. As the subjects' age increased, the figure of Hs, D, Pt, Sc, Si, AVD, and SZD increased, while the HST figure decreased. Conclusions : By the above results, we can assume that the sibling relations and the order one is born in the family does not affect one's personality much in grown-ups; whereas the gender and age affect the personality.