• 한국임상수의학회지
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• 제32권4호
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• pp.380-384
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• 2015

The relationships between end-tidal partial pressure of carbon dioxide ($P_{ET}CO_2$) and arterial partial pressure of carbon dioxide ($P_aCO_2$), and between $P_{ET}CO_2$ and blood pH in isoflurane-anesthetized cinereous vultures were evaluated. Nine cinereous vultures (weighing 7.3-9.5 kg) were anesthetized via mask with isoflurane, intubated, and connected to a mechanical ventilator. By mechanical ventilator, respiratory rate was altered to achieve a $P_{ET}CO_2$ randomly selected among 30 to 35 mmHg. $P_aCO_2$ and blood pH were determined in serial arterial samples and compared with concurrent $P_{ET}CO_2$ measured with a sidestream capnography. Thirty six samples, nine cinereous vultures were tested 4 times each, were used to determine correlations coefficients between $P_aCO_2$ and $P_{ET}CO_2$, and between $P_{ET}CO_2$ and blood pH. Strong correlations were observed between $P_aCO_2$ and $P_{ET}CO_2$ (r = 0.951, P = 0.000, y = 1.679x - 30.236, $R^2=0.905$) as well as between $P_{ET}CO_2$ and blood pH (r = -0.728, P = 0.000, y = -0.024x + 8.389, $R^2=0.530$) with Pearson's correlation coefficient and simple linear regression. These results suggest that the capnography used provided a sufficiently accurate estimation of $P_aCO_2$ and blood pH. Thus, the monitoring of $P_{ET}CO_2$ can be useful method to prevent hypoventilation for cinereous vultures receiving mechanical ventilation.

• 한국엔터테인먼트산업학회논문지
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• 제14권4호
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• pp.355-363
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• 2020

이 연구는 만성요통환자의 호흡패턴이상과 통증관의 상관관계를 알아보기 위해 실시하였다. 104명의 만성요통환자의 날숨끝 이산화탄소농도(EtCO₂)와 호흡률을 카프노그래프를 통해 측정하였고, 호흡유지시간과 Nijmegen Questionnaire, 가슴우리 움직임거리, 시각적사상척도를 추가로 평가하였다. Pearson's 검사로 상관관계를 분석하였으며, 결과는 EtCO₂와 BHT, 가슴우리 움직임거리에서 통계적으로 유의한 상관관계를 보였으며(r=.302, r=.281)(p<.01), RR과 VAS에서 낮은 음의 상관관계(r=-.253,-.200)(p <.05)를 보였다. RR과 NQ에서도 유의한 상관관계를 보였으며(r=.237)(p<.05). 가슴우리 움직임거리와 VAS사이에서는 낮은 음의 상관관계를 보였다(r=-.370)(p<.01). 가슴 호흡과 가로막호흡 사이의 EtCO₂, RR, BHT, 가슴우리 움직임거리, 그리고 VAS에서 통계적으로 유의한 차이가 있었으며(p<.05), NQ에서는 유의한 차이가 없었다(p>.05). 만성요통환자의 EtCO₂, BHT, 가슴우리움직임거리, RR, 그리고 VAS 사이에는 상관관계가 있었다. 또한, RR과 NQ, 흉부 소풍과 VAS 사이에는 상관관계를 보였다. 결과적으로 호흡패턴이상과 통증 사이에는 밀접한 관계가 있었으며, 가슴호흡과 가로막호흡의 비교에서 EtCO₂, RR, BHT, 가슴우리 움직임거리 및 VAS에서 유의한 차이가 있었다. 이것은 만성요통환자의 재활 및 통증 관리에서 호흡패턴중재가 기초자료로 사용될 수 있을 것으로 사료된다.

• 대한화장품학회지
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• 제33권3호
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• pp.203-208
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• 2007

천연물로부터 새로운 미백 화장품 소재를 개발하기 위해 참나물(P. brachycarpa)을 선택하여 B16 멜라노마 세포에서 tyrosinase 활성 저해 효과, melanin 생성 저해 효과를 측정하였다. 먼저 참나물추출물의 4가지 극성별 용매 분획화 실험을 실시하였으며, 항산화효과와 티로시나아제 저해효과를 측정하였다. 참나물의 미백 활성 메카니즘을 알아보기 위해 Western blotting과 RT-PCR을 이용하여 tyrosinase, TRP-1, TRP-2의 단백질 발현과 mRNA 변화를 연구하였다. 또한, HaCaT 각질형성세포에서 UVB 조사 후 엔도세린-1(ET-1)의 발현은 사람 ET-1 항체를 이용하여 quantitative enzyme immunoassay(EIA)로 측정하였다. 그 결과 참나물추출물과 4가지 분획(hexane, EtOAc, butanol and aqueous)은 100 ${\mu}g/mL$ 농도에서 각각 87.2, 2.5, 97.2, 80.5, 49.8%의 프리라디칼 소거효과를 나타내었으며, tyrosinase 저해효과는 100 ${\mu}g/mL$ 농도에서 각각 18.3, 15.1, 55.4, 13.1, 0%로 나타났다. 각 극성별 분획 중 EtOAc 분획 100 ${\mu}g/mL$ 농도에서 58% 이상의 가장 우수한 멜라닌 생성 저해 효과가 나타났다. 참나물 EtOAc 분획은 B16 멜라노마 세포에서 티로시나아제 활성 및 발현을 모두 저해하였으며, RT-PCR 결과에서도 tyrosinase, TRP-1의 mRNA 발현 저해효과가 우수하게 나타났다. 또한 HaCaT 각질형성세포에서 UVB 조사 후 생성된 엔도세린-1의 생성 실험에서도 참나물 EtOAc 분획 $12.5{\sim}50{\mu}g/mL$ 농도에서 엔도세린-1의 생성이 컨트롤에 비해 40% 정도로 우수하게 저해되었다. 결론적으로 참나물추출물은 멜라닌 합성과정에서 우수한 tyrosinase 저해효과와 엔도세린-1 발현저해효과를 가지는 새로운 천연 미백 소재로 적용될 수 있을 것이라 기대된다.

• 한국환경생태학회지
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• 제24권1호
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• pp.62-68
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• 2010

지구온난화 조건 하에 한국특산식물 섬자리공과 귀화식물 미국자리공의 발아, 식물계절 그리고 잎의 형태학적 변화 를 알아보기 위해 $CO_2$농도(+380~420ppm)와 온도($+3.0^{\circ}C$)를 증가시킨 $CO_2$+온도상승구(EC-ET)와 대조구(AC-AT)에서 실험하였다. 두 종의 발아율은 $CO_2$+온도상승구에서 높았으며 발아개시일 역시 빨랐다. 그리고 섬자리공은 미국자리공에 비해 낮은 발아율 보였다. 섬자리공은 2년 통안 영양생장만 일어난 반면 미국자리공은 2008년과 2009년 한 해에 영양생장과 생식생장을 모두 하였다. 두 종의 식물계절은 $CO_2$농도와 온도가 증가할수록 개업, 꽃대형성, 개화, 열매발달과 성숙시기가 빨라졌으며 잎의 노화 시기는 늦춰졌다. 섬자리공의 잎몸 길이는 $CO_2$농도와 온도에 대한 영향을 받지 않았다. 반면 미국자리공은 $CO_2$+온도상승구에서 높았다. 잎몸 폭 길이는 두 종 모두 $CO_2$+온도상승구에서 높았다. 두 종의 총 잎 수는 구배별 차이가 없었다. 그리고 비엽면적은 섬자리공이 대조구에서 높은 반면 미국자리 $CO_2$+온도상승구에서 높았다. 이상으로 볼 때, 지구온난화가 되면 미국자리공이 섬자리꽁보다 $CO_2$농도와 증가에 대하여 더 민감하게 반응을 할 것으로 판단된다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권4호
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• pp.301-305
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• 2001

The co-expression of the arg U gene in a double-vector expression system of recombi-nant Escherichia coli BL22(DE3)[pET-IEN2a+pAC-argU] significantly enhanced the production level of reconminant human interferon -$\alpha$2a(rhIFN-$\alpha$2a) in high cell density cultures, compared to a recombinant E. coli culture containing only the single expression vector, pET-IEN2a. The dry cell mass concentration increased to almost 100 g/L, and more than 4 g/L of rhIFN-$\alpha$2a was accumu-lated in the culture broth. Evidently, the synthesis of rhIFN-$\alpha$2a was strongly dependent on the pre-induction growtih rate and more efficient at a higher specific growth rate. The additional sup-ply of tRN $A^{Arg(AGG/AGA)}$ enhanced the expression level of the rhIFN-$\alpha$2a gene in the early stage of the post-induction phase, yet thereafter the specific production rate of rhIFN-$\alpha$2a rapidly de-creased due to severe segregational instability of plasmid vector pET-IEN2a. It would appear that the plasmid instability with only occurred to pET-IEN2a in the double vector system, was re-lated to the effect of translational stress due to the over expression of rhIFN-$\alpha$2a.

• 설비공학논문집
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• 제20권11호
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• pp.733-738
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• 2008

Due to environmental concerns $CO_2$ has been reintroduced as a potential candidate to replace HFCs in refrigeration systems. Oils are always required in a vapor-compression cycle, and thus it is necessary to precisely estimate the thermodynamic mixture properties of $CO_2$-lubricant oil. In the present study, the density and the viscosity of the mixture was calculated by the Redlich and Kwong type EoS and the modified Peng and Robinson type viscosity EoS, respectively. The viscosity model was based on the similarity between P-v-T and T-$\mu$-P relationships. The predicted results were compared with the experimental data of Pens ado et al. whose test conditions were 100$\sim$650 bar of pressure and 303 K$\sim$353 K of temperature with the $CO_2$-POEs mixtures under 92.2 wt.% and 83.3 wt.% of $CO_2$ concentration. The mean deviations of the mixture density were 7.93% and 8.32% for 92.2 wt.% and 83.3 wt.% of $CO_2$ concentration, respectively. Concerning the viscosity, the mean deviations were 4% and 10% for 92.2 wt.% and 83.3 wt.% of $CO_2$ concentration under the Pensado et al.'s test conditions.

• 센서학회지
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• 제13권4호
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• pp.258-263
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• 2004

In this study, we carried out a basic study for the development of optical transcutaneous $pCO_{2}$ gas sensor and analyzer using non-invasive method. The basic principle of $pCO_{2}$ measurement is adapted Beer lambert's law and embodied the system using NDIR method. This measuring system was composed of a IR lamp, a optical filter, a optical reaction chamber, pyroelectric sensor and a signal process. We measured $EtCO_{2}'s$ concentration in basis step instead of $pCO_{2}$ gas that can collect by inflicting heat in outer skin. We minimize the size of optical reaction chamber which takes up the largest volume, to make the portable sensor. We made optical reaction chamber in Si wafer using MEMS technology and the optical reaction chamber was shortened to 2 mm and we carried out an experiment. When we injected the $EtCO_{2}$ to the inside of the optical reaction chamber, we could confirm change of 4.6 mV. The system response time was within 2 second that is fairly fast.

• Bulletin of the Korean Chemical Society
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• 제17권2호
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• pp.138-142
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• 1996

The transition metal carbonylate, PPN+(${\eta}^5-MeCp)Mn(CO)_2Cl^-$ undergoes a novel ligand substitution reaction with PR3 (R=Me, Et, OEt, $C_6H_5$ in THF at elevated temperatures (40 $^{\circ}C$ up to 60 $^{\circ}C)$ under the pseudo-first-order reaction conditions (usually 20-fold excess of PR3 with respect to metal carbonylate concentrations) where chloride is displaced by PR3. The reaction follows overall first order dependence on [(${\eta}^5-MeCp)Mn(CO)_2Cl^-$]; however, the negative entropy changes of activation (${\Delta}S^{\neq}$=-19.3 e.u. for $P(OEt)_3$; ${\Delta}S^{\neq}$=-16.4 e.u. for $PPh_3$) suggest the existence of the intermediate, ((η3-MeCp)Mn(CO)2(THF)Cl-, which eventually transforms to the product (${\eta}^5-MeCp)Mn(CO)_2(PR_3)$.

• 대한치과마취과학회지
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• 제6권2호
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• pp.89-97
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• 2006

Background: The aim of this study was to examine the difference of $SpO_2$, PR, $EtCO_2$, RR with submucosal injection of midazolam to oral chloral hydrate and hydroxyzine for pediatric patients Methods: Thirty two sedation cases were performed in this study. Patients were randomly classified into one group taking oral CH (60 mg/kg). hydroxyzine (1 mg/kg) and submucosal injection of midazolam (0.1 mg/kg) and the other group recieving oral CH (50 mg/kg), hydroxyzine (1 mg/kg) and submucosal injection of midazolam (0.2 mg/kg). For evaluating the depth of sedation. data including saturation percentage of oxygen ($SpO_2$), pulse rate (PR), end-tidal carbon dioxide ($EtCO_2$), respiratory rate (RR) and the behavior scale were checked every 2 minutes and were collected for only 40 minutes from the beginning of treatment and were analyzed using Two independent sample T-test. Results: Analysis showed no significant difference in the mean $SpO_2$, PR, $EtCO_2$, RR during sedation between two groups (P > 0.05). The values of $SpO_2$, PR, $EtCO_2$ and RR for both groups remained within the normal values. Conclusions: The results of this present study indicate that combination of oral CH, hydroxyzine, nitrous oxide gas inhalation and submucosal injection of midazolam improved the sedation quality without compromising safety.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.49-49
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• 2021

The roots of reed (Phragmites australis) were used in traditional medicine to treat respiratory problems, including symptoms such as high fever and cough. In this study, we identified the active ingredient from 70% EtOH reed root extract, and evaluated the whitening, wrinkle improvement and moisturizing effects. The content of p-coumaric acid, the active ingredient of the roots of P. australis, was slightly lower in 70% EtOH extract than in 100% EtOH extract. However, 70% EtOH reed root extract showed similar or higher effect in reducing power, DPPH, hydrogen peroxide scavenging, and nitric oxide scavenging activity compared to 100% EtOH extract. Moreover, 70% EtOH reed root extract markedly inhibited melanogenesis in B16F10 cells treated with α-melanocyte-stimulating hormone. 70% EtOH reed root extract significantly inhibited the mRNA expression of matrix metalloproteinase-1 (MMP-1) and reduced elastase activity in HDF human dermal fibroblasts. In addition, 70% EtOH reed root extract ameliorated hyaluronic acid synthase-2 (HAS-2) expression induced by ultraviolet B (UVB) stimulation in HaCaT keratinocytes. The results of this study suggest that 70% EtOH reed root extract has potential as a functional cosmetic material related to whitening, wrinkle improvement, and moisturizing.