• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.371-377
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• 2009

The objectives of the present study were to investigate the effects of different dietary ratios of whole-crop corn silage and alfalfa hay on nitrogen (N) digestion, duodenal flow and metabolism across the portal-drained viscera (PDV) of growing beef steers, and to elucidate their relationships. Four steers (236${\pm}$7 kg BW) fitted with duodenal cannulae and chronic indwelling catheters into the portal and mesenteric veins and abdominal aorta were used in a 4${\times}$4 Latin square design. Animals were fed (at 12-h intervals) the 4 diets consisting of whole-crop corn silage (C) and alfalfa hay (A) in 80:20 (C8A2), 60:40 (C6A4), 40:60 (C4A6) and 20:80 (C2A8) ratios of which dietary crude protein (CP) was 10.5, 12.0, 13.5 and 15.0% of dry matter (DM), respectively. Feeding level was restricted to 95% of ad libitum intake to measure N digestion, blood flow and net flux of N across the PDV. Digestibility of DM and neutral detergent fiber and digestible energy intake linearly increased as the ratio of alfalfa hay increased. The N intake, duodenal flow and intestinal disappearance increased linearly with increasing alfalfa hay. Arterial and portal concentrations of ${\alpha}$-amino N showed a quadratic response to increasing levels of alfalfa hay and were the highest in steers fed the C6A4 diet. The net PDV release of ${\alpha}$-amino N and ammonia N increased linearly with increasing alfalfa hay, but urea N uptake by PDV did not differ among diets. As a percentage of apparently digested N in the total gut, net PDV release of ${\alpha}$-amino N linearly decreased from 66 to 48% with increasing alfalfa hay. Conversely, net PDV recovery of ${\alpha}$-amino N to intestinal N disappearance varied with increasing alfalfa hay accounting for 49, 50, 58 and 61% on C8A2, C6A4, C4A6 and C2A8 diets, respectively. Net PDV uptake of urea N, relative to apparently digested N, linearly decreased from 81 to 25% as alfalfa hay increased from 20 to 80% of DM intake. Considering PDV uptake of urea N, microbial efficiency and conversion of total tract digested N to PDV ${\alpha}$-amino N net supply, a diet consisting of 80% whole-crop corn silage and 20% alfalfa hay (10.5% CP) was the best, while considering the quantities of intestinal N disappearance and ${\alpha}$-amino N absorption, a diet of 20% whole-crop corn silage and 80% alfalfa hay (15% CP) would be preferred. The proportion of ${\alpha}$-amino N recovered by PDV relative to the intestinal N disappearance may vary with energy intake level of mixed forage diets.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.2
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• pp.208-217
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• 2018

Objective: Examine the effects of supplementing bahiagrass hay (BG) with potentially anthelmintic quantities of hays of perennial peanut (PEA) or sericea lespedeza (LES) or seeds of velvet bean (Mucuna pruriens L.; MUC) or papaya (PAP) on the intake and nutritive value (Experiment 1), and the performance and parasite burden (Experiment 2) of goats. Methods: In Experiment 1, 38 male goats ($27.4{\pm}5.7kg$ body weight) were randomly assigned to each of 5 treatments: i) BG alone and BG plus; ii) PEA; iii) LES; iv) MUC; and v) PAP. Goats were fed for ad libitum consumption and adapted to the diets for 14 d followed by 7 d of measurement. The PEA, LES, MUC (50%, 50%, and 10% of the diet dry matter [DM], respectively), and PAP (forced-fed at 10 g/d) were fed at rates that would elicit anthelmintic effects. In Experiment 2, goats remained in the same treatments but were allocated to 15 pens (3 pens per treatment) from d 22 to 63. All goats were infected with parasites by grazing an infected bahiagrass pasture from 0800 to 1500 h daily and then returned to the pens. Results: Dry matter intake tended to be greater in goats fed PEA and LES than those fed BG (757 and 745 vs 612 g/d, respectively). Digestibility of DM (59.5% vs 54.9%) and organic matter (60.8% vs 56.0%) were greater in goats fed MUC vs BG, respectively. In Experiment 2, feeding PAP, LES, and PEA to goats reduced nematode fecal egg counts by 72%, 52%, and 32%, reduced abomasal adult worm counts by 78%, 52%, and 42%, and decreased plasma haptoglobin concentrations by 42%, 40%, and 45% relative to feeding BG alone, respectively. Conclusion: Supplementation with PEA, LES, and PAP decreased the parasite burden of goats but did not increase their performance. PAP was the most effective anthelmintic supplement.

• Tuberculosis and Respiratory Diseases
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• v.44 no.2
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• pp.349-359
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• 1997

Background : The signal pathways and their precise roles for acute respiratory distress syndrome caused by endotoxin (ETX) has not been established. Since there has been several in vitro experiments suggesting that activation of protein kinase C (PKC) pathway may be responsible for endotoxin-induced inflammatory reaction, we performed in vivo experiments in the rats with the hypothesis that PKC-inhibition can effectively prevent endotoxin-induced acute lung injury. Methods : We studied the role of PKC in ETX-induced ALI using PKC inhibitor (staurosporine, STP) in the rat Specific pathogen free male Sprague-Dawley weighted from 165 to 270g were used for the study. Animals were divided into the normal control (NC)-, vehicle control (VC)-, ETX-, PMA (phorbolmyristateacetate)-, STP+PMA-, and STP+ETX-group. PMA (50mg/kg) or ETX (7mg/kg) was instilled through polyethylen catheter after aseptic tracheostomy with and without STP (0.2mg/kg)-pretreatment STP was injected via tail vein 30min before intratracheal injection (IT) of PMA or ETX. Bronchoalveolar lavage (BAL) was done 3-or 6-hrs after IT of PMA or ETX respectively, to measure protein concentration, total and differential cell counts. Results : The results were as follows. The protein concentrations in BALF in the PMA- and ETX-group were very higher than that of VC-group (p<0.001). When animals were pretreated with STP, the %reduction of the protein concentration in BALF was $64.8{\pm}8.5$ and $30.4{\pm}2.5%$ in the STP+PMA- and STP+ETX-group, respectively (p = 0.028). There was no difference in the total cell counts between the PMA-and VC-group (p = 0.26). However the ETX-group showed markedly increased total cell counts as compared to the VC- (p = 0.003) and PMA-group (p = 0.0027), respectively. The total cell counts in BALF were not changed after pretreatment with STP compared to the PMA- (p = 0.22) and ETX-group (p = 0.46). The percentage of PMN, but not alveolar macrophage, was significantly elevated in the PMA-, and ETX-group. Especially in the ETX-group, the percentage of PMN was 17 times higher than that of PMA (p < 0.001). The differential cell counts was not different between the PMA and STP+PMA On the contrary the STP+ETX-group showed decreased percentage of PMN (p = 0.016). There was no significant relationship between the protein concentration and the total or differential cell counts in each group. Conclusion : Pretreatment with PKC-inhibitor (staurosporine) partially but significantly inhibited ETX-induced ALI.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.537-546
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• 2005

An experiment was conducted to determine the effect of supplementation of chlortetracycline (CTC,110ppm), copper sulfate(Cu, 125ppm) and two levels of probiotics(Prob I, 0.04%, Prob II, 0.1%), Bacillus licheniformis and Bacillus subtilis) on growth performance, nutrient digestibility and manure characteristics in growing pigs. A total of 50 pigs that averaged 20.78$\pm$0.35kg BW and 50$\pm$2.3d age were allocated in a randomized block design with two pigs per pen and 5 pens per treatment. Pigs and feeders were weighed 10-days interval for the 40-d trials to determine ADG, ADFI and feed:gain ratio(F:G). Average daily gain, feed intake, feed/gain and nutrient digestibility were not improved(P>0.10) by the supplementation of CTC, Cu and two levels of probiotics. There were significant(P<0.05) Cu effects on digestibility of crude protein, and probiotics effects on digestibilities on organic matter, crude protein and energy between first 20 days and subsequent 20 days. Fecal concentrations of copper were highly increased(P<0.001) by the copper supplementation. Total bacteria and coliform counts were not altered by the supplementation of CTC, Cu and two levels of probiotics. Ammonia emission from slurry, measured during first 3 weeks, was reduced (P<0.001) in pigs fed diet with 125 ppm copper from copper sulfate. Results indicate that CTC, Cu and Probiotics supplementation had a little or not positive effect on grower pig performance under sound environmental conditions. Further studies may be warranted to investigate the effects of dietary copper-either reduced or in combination with dietary ammonia control agents-on the ammonia emission characteristics of swine manure.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.4
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• pp.487-498
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• 2006

Total 438 precipitation samples were collected in Jeju City between 1997 and 2005, and their major ionic components were analyzed. The comparison tests using ion balance, electric conductivity and acid fraction were performed. It was found their correlation coefficients were in the range of 0.977$\sim$0.994, indicating the good quality of collected dam. The volume-weighted mean pH and electric conductivity were 4.8 and 23.0 $\mu$S/cm, respectively. with the ionic strength of 0.23$\pm$0.20 mM. The marine ($Na^+$, $Mg^{2+}$, and $CI^-$), anthropogenic (nss$SO_4{2-}$, $NO_3^-$, and $NH_4^+$) and soil (nss-$Ca^{2+}$) species have contributed to the ionic components of precipitation samples with 43$\sim$74%, 16$\sim$37% and $\sim$5%, respectively. The seasonal variations of $NO_3^-$ and nss-$SO_4^{2-}$ showed a distinct seasonality with higher concentrations in winter than summer, indicating an increase of fossil fuel consumption and a possibility of long-range transport of those pollutants from continental area along the dominant winter westerly. The levels of nss-$Ca^{2+}$ also were appeared the highest in winter and increased comparatively in spring season. possibly due to the soil influences including the Asian Dust. The acidification contribution of nss-$SO_4^{2-}$ and $NO_3^-$ showed 88$\sim$96%, and the free acidity was in the range of 6.0$\sim$40.1%. Interestingly, the backward trajectories for the case of upper 10% nss-$SO_4^{2-}$ and $NO_3^-$ levels have passed through the China continent before their arrival to Jeju. The precipitation of pH below 4.5 has been occurred frequently when the trajectory's path lied over the China continents. On the other hand, the air masses from the North Pacific area were characterized by lower 10% of nss-$SO_4^{2-}$- and $NO_3^-$ concentration, which demonstrated that air mass from the North Pacific was the cleanest among air masses moved to Jeju.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.12
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• pp.1914-1922
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• 2019

Objective: The study was conducted to evaluate the effect of stocking density and alphalipoic acid (ALA) on the growth performance, feed utilization, carcass traits, antioxidative ability and immune response of broilers. Methods: A total of 1,530 22-day-old male broilers (Arbor Acres) with comparable body weights ($731.92{\pm}5.26$) were placed into 18 cages ($2.46{\times}2.02m$) in groups of 75 birds ($15birds/m^2$, $37.5kg/m^2$; low stocking density [LD]), 90 birds ($18birds/m^2$, $45.0kg/m^2$; high stocking density [HD]) and 90 birds with 300 mg/kg ALA added to the basal diet ($18birds/m^2$, $45.0kg/m^2$; HD+ALA, high stocking density+${\alpha}$-lipoic acid); each treatment was represented by 6 replicates. The experimental period was 3 weeks. Results: The results showed that the high stocking density regimen resulted in a decreased growth, feed conversion ratio, carcass weight, thigh yield and bursa weight relative to body weight (p<0.05) on d 42. The abdominal fat yield in the HD+ALA group was lower (p = 0.031) than that of the LD group at 42 d. The superoxide dismutase and glutathione peroxidase activities in serum were increased, and malondialdehyde content decreased after adding ALA product (p<0.05) on d 42. Additionally, the serum concentrations of immunoglobulin A (IgA) and IgG were decreased (p<0.05) and the level of diamine oxidase was higher (p<0.01) in the HD group on d 42. Conclusion: The high stocking density significantly decreased broiler growth performance, feed utilization and carcass traits, increased physiological and oxidative stress and induced intestinal mucosal injury. The supplementation of ALA product in broiler diet at 300 mg/kg may reduce the adverse effects of high stocking density-mediated stress by maintaining the antioxidant system and humoral immune system.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.5
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• pp.755-762
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• 2018

Objective: The objective of the study was to determine the influence of varying fibrous diets on fecal characteristics of growing pigs. Methods: A total of 104 pigs (initial weight $18{\pm}2.0kg$) were used in the study. They were housed in individual pens and fed on diets containing maize cob, grass hay, lucerne hay, maize stover, and sunflower husk. These fibers were included at 0, 80, 160, 240, 320 and 400 g/kg. Fecal and urine samples were collected. Results: Fecal output was largest amongst pigs fed on diets containing grass hay and maize stover (p<0.05). Nitrogen content was highest in feces from pigs fed on sunflower husk (p<0.05). Pigs fed on diets containing maize stover and maize cobs produced the largest concentrations of short chain fatty acids. Acetate concentration was high in feces of pigs fed maize stover than those fed grass hay and lucerne hay (p<0.05). As the level of fiber inclusion increased, fecal consistency and nitrogen content increased linearly (p<0.05). Urea nitrogen decreased as the inclusion level increased across all the fibers (p<0.05), with maize cobs containing the largest content of urea nitrogen. As dietary fiber content increased, fecal nitrogen content also increased (p<0.05). Conclusion: It was concluded that different fiber sources influence fecal characteristics, thereby having different implications on pig waste management. It is vital to monitor fiber inclusion thresholds so as to easily manage environmental pollutants such as butyrate that contribute to odors.

• Journal of Environmental Science International
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• v.27 no.1
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• pp.27-38
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• 2018

This study was carried out in the Bukhan River in the summer of 2014 and 2015, to identify the relationship between geosmin and the morphological changes in Anabaena. Identification of Anabaena was conducted using morphological and molecular analyses. Anabaena in this study was similar to Anabaena circinalis, A. crass, and A. spiroides with regard to regular coils, vegetative cell, akinete shape, and size, hoever, it was distinguishabl from A. crass and A. spiroides because of its larger trichome coil size. Additionally, the sequences of phycocyanin (PC) gene from Anabaena showed a 99% genetic similarity with A. circinalis NIES-1647 strain. The coil diameter of trichome ranged from 106 to $899{\mu}m$, and the diameter and abundance showed an insignificant positive correlation (r=0.544, p<0.05). The result of relationship between the coil diameter and the cell number per 360-degree rotation was kept at $33.8{\pm}5.2$ cells per $100{\mu}m$ diameter despite variable diameter. The average geosmin concentrations in 2014 and 2015 were investigated to be 99 ng/L and 35 ng/L, respectively. A. circinalis cell density contributed considerably to the change in geosmin and was positively correlated with geosmin concentration (2014; r=0.599, p<0.01, 2015; r=0.559, p<0.01). Our results suggest that geosmin and coil diameter could be estimated with the help of cell density.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.4
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• pp.465-474
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• 2010

The effects of four types of tropical protein-rich trees on nutrient digestibility, nitrogen (N) balance, urinary purine derivative (PD) excretion and blood metabolites in four Thai Brahman cattle (290${\pm}$2.5 kg) were studied. The animals were fed twice daily, with each feeding consisting of 1 kg (fresh weight) rice straw and one of the four dietary supplements: i) 1.98 kg oven-dried rain tree pods (RTP) and 20 g premix (RTPP), ii) 980 g RTP and 1 kg sun-dried leucaena leaves and 20 g premix (LLRT), iii) 980 g RTP and 1 kg sun-dried cassia leaves and 20 g premix (CLRT) and iv) 980 g RTP and 1 kg sun-dried mulberry leaves and 20 g premix (MLRT). The apparent dry matter (DM) and organic matter (OM) digestibilities were higher (p<0.05) in cattle fed the CLRT supplement than in those fed the other supplements, whilst the apparent digestibility of neutral detergent fibre (NDF) was higher (p<0.05) in cattle fed the CLRT and MLRT supplements than in those fed the other supplements. The N-balance of cattle fed LLRT and CLRT supplements was higher (p<0.05) than in cattle fed RTPP and MLRT supplements, whilst the apparent digestibility of N was highest (p<0.05) in cattle fed RTPP supplement, compared to the other supplements. Allantoin and PD excretion in the urine, and the ratios of allantoin/DOMI and PD/DOMI were higher (p<0.05) in cattle fed RTPP and MLRT than for those fed LLRT and CLRT supplements. Plasma ${\beta}$-hydroxy butyrate (${\beta}$-HBA) and insulin concentrations were higher (p<0.05) in cattle fed RTPP supplement than in those fed the other supplements. The study demonstrated the value of using local multipurpose trees (MPTs) to improve Brahman cattle feeding systems in the tropics.

• Journal of Korean Society for Atmospheric Environment
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• v.24 no.6
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• pp.629-640
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• 2008

This study investigates long-term trends and characteristics of aerosol optical depth ($\tau_a$) and Angstrom exponent (${\AA}$) around Korea in order to understand aerosol effects on the regional climate change. The analysis period is mainly from 1999 to 2006, and the analysis sites are Anmyun and Gosan, the background monitoring sites in Korea, and two other sites of Xianghe in China and Shirahama in Japan. The annual variations of $\tau_a$ at Anmyun and Gosan have slightly systematic increasing and decreasing trends, respectively. $\tau_a$ at Anmyun shows more substantial variation, probably because of it's being closer and vulnerable to anthropogenic influence from China and/or domestic sources than Gosan. Both values at Gosan and Anmyun are approximately 1.5 times greater than those at Shirahama. The monthly variation of $\tau_a$ exhibits the highest values at late Spring and the lowest at late-Summer, which are thought to be associated with the accumulation of fine aerosol formed through the photochemical reaction before the Jangma period and the scavenging effect after the Jangma period, respectively. Meanwhile, the episode-average $\tau_a$ for the Yellow dust period increases 2 times greater than that for the non-Yellow dust period. A significant decrease in ${\AA}$ for the Yellow dust period is attributable to an increase in the loading of especially the coarse particles. Also we found no weekly periodicity of $\tau_a$'s, but distinct weekly cycle of $PM_{10}$ concentrations, such as an increase on weekdays and a decrease on weekends at Anmyun and Gosan. We expect these findings would help to initiate a study on aerosol-cloud interactions through the combination of surface aerosol and satellite remote sensing (MODIS, Calipso and CloudSat) in East Asia.