• 생명과학회지
• /
• 제24권4호
• /
• pp.343-351
• /
• 2014

Phosphoinositide 3-kinase (PI3K)는 항산화 제어반응, 심근세포 성장, 및 세포 내 특수반응 뿐만 아니라 세포분화, 생장, 운동, 식균 및 내항작용, 세포 골격유지에 관여하는 등 세포 신호체계에서 핵심 역할을 하는 효소이다. PI3K는 세 그룹으로 나누어지며 type I PI3K는 leukocyte에서 우선적으로 발현되고 G-proteins의 ${\beta}{\gamma}$ subunits에 의해서 활성화 된다. 본 연구에서는 넙치(Paralichthys olivaceus)의 $PI3K{\gamma}$를 암호화하는 cDNA를 클로닝하였다. 넙치의 $PI3K{\gamma}$는 1,341 bp 염기로 구성되는 한 개의 ORF를 가지며 이 단백질은 447 아미노산으로 구성되어있다. $PI3K{\gamma}$는 zebrafish의 $PI3K{\gamma}$와 89.6%, mouse와는 84.7%, Norway rat와는 84%, human의 $PI3K{\gamma}$와는 74.9%가 아미노산 상동성을 나타내었다. $PI3K{\gamma}$유전자의 대장균에서 발현을 위하여 pET-44a(+)-PI3K 재조합 DNA를 구축하여 대장균에서 발현시킨 결과 49 kDa의 재조합 단백질이 과발현 됨을 확인 할 수 있었다. His-tag affinity chromatography를 이용하여 $PI3K{\gamma}$단백질을 순순 분리하였으며 wortmannin을 이용하여 $PI3K{\gamma}$의 활성을 분석하였다.

• BMB Reports
• /
• 제46권2호
• /
• pp.103-106
• /
• 2013

Phosphoinositide 3-kinases (PI3Ks) play key roles in synaptic plasticity and cognitive functions in the brain. We recently found that genetic deletion of $PI3K{\gamma}$, the only known member of class IB PI3Ks, results in impaired N-methyl-D-aspartate receptor-dependent long-term depression (NMDAR-LTD) in the hippocampus. The activity of RalA, a small GTP-binding protein, increases following NMDAR-LTD inducing stimuli, and this increase in RalA activity is essential for inducing NMDAR-LTD. We found that RalA activity increased significantly in $PI3K{\gamma}$ knockout mice. Furthermore, NMDAR-LTD-inducing stimuli did not increase RalA activity in $PI3K{\gamma}$ knockout mice. These results suggest that constitutively increased RalA activity occludes further increases in RalA activity during induction of LTD, causing impaired NMDAR-LTD. We propose that $PI3K{\gamma}$ regulates the activity of RalA, which is one of the molecular mechanisms inducing NMDAR-dependent LTD.

• Parasites, Hosts and Diseases
• /
• 제37권4호
• /
• pp.285-287
• /
• 1999

Changes in the expression level of splenocyte $IFN-{\gamma}$mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of $IFN-{\gamma}$had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with p. westermani, the levels of $IFN-{\gamma}$ mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.

• 한국광학회지
• /
• 제10권3호
• /
• pp.188-194
• /
• 1999

Mitsunobu 반응을 이용하여 전기광학계수 $\gamma_{33}$크고 열적 .시간적으로 안정적인 폴리이미드(polyimide)계고분자 용액인 PI-SOT(폴리이미드계 4-[N,N-bis(hydroxyethyl)amino­4'­($\beta$­cyano-$\beta$­methylsulfonyl)vinyl]azobenzene)고분자를 합성하였다. C.C. Teng의 단순반사법을 이용하여 코로나 폴링으로 극성 배향시킨 PI-SOT 박막의 전기광학계수 $\gamma_{33}$의 열안정성 및 시간안정성을 파장 632.8 nm와 852nm에서 측정하였다. 그 결과 상온에서 전기광학계수$\gamma_{33}$은 파장 632.8nm서 25.12 pm/V이고, 852 nmsns 5.40pm/V였다. 이 값들은 상온에서 60일 이상 장기간 안정적으로 유지되었으며, $100^{\circ}C$의 고온에서도 10시간내에서 6% 이내로 안정된 값을 유지하였다.

• Bulletin of the Korean Chemical Society
• /
• 제34권9호
• /
• pp.2829-2832
• /
• 2013

• Journal of applied mathematics & informatics
• /
• 제23권1_2호
• /
• pp.321-327
• /
• 2007

We say that a triangle ${\Delta}$ tiles the polygon ${\rho}\;if\;{\rho}$ can be decomposed into finitely many non-overlapping triangles similar to ${\Delta}$. Let ${\rho}$ be a parallelogram with angles ${\delta}\;and\;{\pi}-{\delta}\;(0<{\delta}{\leq}{\pi}/2)$ and let ${\Delta}$ be a triangle with angles ${\alpha};{\beta},\;{\gamma}\;({\alpha}{\leq}{\beta}{\leq}{\gamma})$. We prove that if ${\Delta}$ tiles ${\rho}$ then either ${\delta}{\in}\;({\alpha},\;{\beta},\;{\gamma},\;{\pi}-{\gamma},\;{\pi}-2{\gamma})\;or\;dimL_{\rho}=dimL_{{\Delta}}$. We also prove that for every parallelogram P, and for every integer n $(where\;n{\geq}2,\;n{\neq}3)$ there is a triangle ${\Delta}$ so that n similar copies of ${\Delta}\;tile\;{\rho}$.

• Biomolecules & Therapeutics
• /
• 제22권4호
• /
• pp.308-313
• /
• 2014

Activator protein-1 (AP-1) is an inducible transcription factor that contributes to the generation of chronic inflammation in response to oxidative and electrophilic stress. Previous studies have demonstrated that the PI3K/Akt1 pathway plays an important role in the transcriptional regulation of AP-1 expression. Although the histone post-translational modifications (PTMs) are assumed to affect the AP-1 transcriptional regulation by the PI3K/Akt pathway, the detailed mechanisms are completely unknown. In the present study, we show that heterochromatin 1 gamma ($HP1{\gamma}$) plays a negative role in TPA-induced c-Jun and c-Fos expression. We show that TPA-induced Akt1 directly phosphorylates $HP1{\gamma}$, abrogates its suppressive function and increases the interaction between histone H3 and 14-3-$3{\varepsilon}$. Collectively, these our data illustrate that the activation of PI3K/Akt pathway may play a permissive role in the recruitment of histone readers or other coactivators on the chromatin, thereby affecting the degree of AP-1 transcription.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
• /
• pp.186-186
• /
• 1994

Many agonists have been known to activate the hydrolysis of membrane phospholipids through the bindings with corresponding receptors on the various cells. Diacylglycerol and inositol 1,4,5-trisphosphate(IP3) generated by the action of phosphoinositide-specific phospholipase C (PI-PLC) are well known second messengers for the activation of protein kinase C and the mobilization of Ca2+ in many cells. Three types of PI-PLC isozyme (${\alpha}$,${\gamma}$, and $\delta$) and several subtrpes for each type have been identified from mammalian sources by purification of enzymes and cloning of their cDNAs. Each type PI-PLC isozyme is coupled to different receptors and mediators, for example, ${\beta}$-types are coupled to the seven-transmembrane-receptors via Gq family of G-proteins and ${\beta}$-types directly to the receptor tyrosine kinases. Specific modulators for the signaling pathway through each type of PI-PLC should be very useful as potential potential candidates for lend substances in developing novel drugs. To establish the sensitive and convenient screening systems for searching modulators on PI-PLC mediated signaling, two kinds of approaches have been tried. (1) Establishment of in vitro assay condition for each type of PI-PLC isozyme: Overexpression by using vaccinia virus and purification of each isozyme was carried out for the preparation of large amounts of enaymes. Optimum and sensitive assay condition for the measurements of PI-ELC activities were established. (2) Development of the cell lines in which each type of PI-PLC is permanently overexpressed: A fibroblast cell line (3T3${\gamma}$1-7) in which PI-PLC-${\gamma}$1 was overexpressed by using pZip-neo expression vector was developed and used for the measurement of PDGF-induced IP3 formation. The responses for IP3 formed in 3T3${\gamma}$1-7 cells by the treatment of PDGF is 8 times more sensitive than those in control cells. 3T3${\gamma}$l-7 cell is useful for the screening of the inhibitors on the PDGF-induced cellular responses from large number of samples in a small volume(50 ${\mu}$l) and short time(5-15 min). Using these systems, we screened hundreds of herb-extracts for the inhibition of PDGF-induced IP3 formation and selected several extracts that showed the inhibition as the candidates for isolation and characterization of active substances. The determination of the acting point of selected extracts or fractions in the PDGF signaling pathway has been analyzing.

• 대한수학회지
• /
• 제47권5호
• /
• pp.967-983
• /
• 2010

Let M be an n-dimensional complete simply connected Riemannian manifold with sectional curvature bounded above by a nonpositive constant $-{\kappa}^2$. Using the cone total curvature TC($\Gamma$) of a graph $\Gamma$ which was introduced by Gulliver and Yamada [8], we prove that the density at any point of a soap film-like surface $\Sigma$ spanning a graph $\Gamma\;\subset\;M$ is less than or equal to $\frac{1}{2\pi}\{TC(\Gamma)-{\kappa}^2Area(p{\times}\Gamma)\}$. From this density estimate we obtain the regularity theorems for soap film-like surfaces spanning graphs with small total curvature. In particular, when n = 3, this density estimate implies that if $TC(\Gamma)$ < $3.649{\pi}\;+\;{\kappa}^2\inf\limits_{p{\in}F}Area(p{\times}{\Gamma})$, then the only possible singularities of a piecewise smooth (M, 0, $\delta$)-minimizing set $\Sigma$ are the Y-singularity cone. In a manifold with sectional curvature bounded above by $b^2$ and diameter bounded by $\pi$/b, we obtain similar results for any soap film-like surfaces spanning a graph with the corresponding bound on cone total curvature.

• Parasites, Hosts and Diseases
• /
• 제40권3호
• /
• pp.119-129
• /
• 2002

Although there are many reports on the splenic (systemic) T cell response after Toxoptasma gondii infection, little information is available regarding the local T cell responses of peritoneal exudate cells (PEC) and gut intraepithelial Iymphocytes (IEL) following peroral infection with bradyzoites. Mice were infected with 40 cysts of the 76K strain of T. gondii, and then sacrificed at days 0, 1, 4, 7 and 10 postinfection (PI). The cellular composition and T cell responses of PEC and IEL were analyzed. The total number of PEC and IEL per mouse increased after infection, but the ratio of increase was higher in IEL. Lymphocytes were the major component of both PEC and IEL. The relative percentages of PEC macrophages and neutrophils/eosinophils increased signiflcantly at day 1 and 4 PI, whereas those of IEL did not change significantly. The percentage of PEC NK1.1 and ${\gamma\delta}T$ cells peaked at day 4 PI (p < 0.0001), and CD4 and $CD8{\alpha}T$ cells increased continuously after infection. The percentages of IEL $CD8{\alpha}$ and ${\gamma\delta}T$ cells decreased slightly at first, and then increased. CD4 and NK1.1 T cells of IEL did not change significantly after infection. $IFN-{\gamma}-producing$ PEC NK1.1 T cells increased significantly from day 1 PI, but the other T cell subsets produced $IFN-{\gamma}$ abundantly thereafter. The proportion of IEL $IFN-{\gamma}-producing$ $CD8{\alpha}$ and ${\gamma\delta}T$ cells increased significantly after infection, while IEL NK1.1 T cells had similar $IFN-{\gamma}$ production patterns. Taken together, CD4 T cells were the major phenotype and the important $IFN-{\gamma}$ producing T cell subsets in PEC after oral infection with T. gondii whereas $CD8{\alpha}T$ cells had these roles in IEL. These results suggest that PEC and IEL comprise different cell differentials and T cell responses, and according to infection route these factors may contribute to the different cellular immune responses.