• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.180-185
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• 2007

The effects of selenium (Se) and germanium (Ge) fertilization on the growth and quality of Chinese cabbages cultivated in spring and autumn and peppers cultivated in spring were investigated. $Se\;(Na_2SeO_4)\;and\;Ge\;(GeO_2)$ were supplied 5, 10, or 20 times in an aqueous solution of 0, 2, 4, or $8mg{\cdot}L^{-1}$ during the cultivation of Chinese cabbages and peppers. The fresh weight of Chinese cabbages increased by Ge fertilization with high concentration. But it was not affected by Se fertilization. The content of vitamin C increased by 10 times application with $4mg{\cdot}L^{-1}$ of Se or Ge. The concentration of Se in Chinese cabbage increased according to increasing concentration of Se fertilization. Se concentration was higher in the outer leaves than in the inner leaves. Se concentration in the mesophyll was higher than that in the midrib. Ge fertilization increased the uptake and concentration of Ge in autumn-cultivated Chinese cabbages. Se and Ge fertilization did not affect the fresh weight of peppers. The content of vitamin C in pepper increased by 20 times application of $2mg{\cdot}L^{-1}$ of Se. Vitamin C content in red peppers was twice as much as in green peppers.

• Journal of Korean Society for Atmospheric Environment
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• v.27 no.3
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• pp.313-325
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• 2011

From October 2009 to June 2010, major greenhouse gases (GHG: $N_2O$, $CH_4$, $CO_2$) soil emission were measured from upland cabbage field at Kunsan ($35^{\circ}$56'23"N, $126^{\circ}$43'14"E), Korea by using closed static chamber method. The measurements were conducted mostly from 10:00 to 18:00LST during field experiment days (total 28 days). After analyzing GHG concentrations inside of flux chamber by using a GC equipped with a methanizer (Varian CP3800), the GHG fluxes were calculated from a linear regression of the changes in the concentrations with time. Soil parameters (e.g. soil moisture, temperature, pH, organic C, soil N) were also measured at the sampling site. The average soil pH and soil moisture were ~pH $5.42{\pm}0.03$ and $70.0{\pm}1.8$ %WFPS (water filled pore space), respectively. The ranges of GHG flux during the experimental period were $0.08\sim8.40\;mg/m^2{\cdot}hr$ for $N_2O$, $-92.96\sim139.38mg/m^2{\cdot}hr$ for $CO_2$, and $-0.09\sim0.05mg/m^2{\cdot}hr$ for $CH_4$, respectively. It revealed that monthly means of $CO_2$ and $CH_4$ flux during October (fall) were positive and significantly higher than those (negative value) during January (winter) when subsoil have low temperature and relatively high moisture due to snow during the winter measurement period. Soil mean temperature and moisture during these months were $17.5{\pm}1.2^{\circ}C$, $45.7{\pm}8.2$%WFPS for October; and $1.4{\pm}1.3^{\circ}C$, $89.9{\pm}8.8$ %WFPS for January. It may indicate that soil temperature and moisture have significant role in determining whether the $CO_2$ and $CH_4$ emission or uptake take place. Low temperature and high moisture above a certain optimum level during winter could weaken microbial activity and the gas diffusion in soil matrix, and then make soil GHG emission to the atmosphere decrease. Other soil parameters were also discussed with respect to GHG emissions. Both positive and negative gas fluxes in $CH_4$ and $CO_2$ were observed during these measurements, but not for $N_2O$. It is likely that $CH_4$ and $CO_2$ gases emanated from soil surface or up taken by the soil depending on other factors such as background concentrations and physicochemical soil conditions.

• Environmental Mutagens and Carcinogens
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• v.25 no.2
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• pp.60-65
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• 2005

The human solute carrier, SLC41Al, is a $Mg^{2}+$ transporter that is regulated by extracellular magnesium. Although intracellular magnesium plays a fundamental role in cellular metabolism, little is known about how $Mg^{2}+$ is taken up and controlled by cells. Magnesium plays a fundamental role in cellular metabolism so that its control within the body is critical. Magnesium homeostasis is principally a balance between intestinal absorption of dietary magnesium and renal excretion of urinary magnesium. The kidney, mainly the distal convoluted tubule, controls magnesium reabsorption. Although renal reabsorption is under the influence of many hormones, selective regulation of magnesium transport is due to intrinsic control involving transcriptional processes and synthesis of transport proteins. Using microarray analysis, identification of the genetic elements involved with this transcriptional control has been begun. SLC41A1(GenBank Accession No. AJ514402), comprises 10 putative transmembrane domains, two of which are highly homologous to the integral membrane part of the prokaryote transports $Mg^{2}+$ and other divalent cations $Sr^2+,\;Zn^2+,\;Cu^2+,\;Fe^2+,\;Co^2+,\;Ba^2+,\;and\;Cd^2+,\;but\;not\;Ca^2+,\;Mn^2+,\;and\;Ni^2+.$ Transport of $Mg^{2}+$ by SLC41Al is rheogenic, voltage dependent, and not coupled to Na or Cl. Expressed SLC41Al transports a range of other divalent cations: $Mg^{2+},\;Sr^{2+},\;Zn^{2+},\;Cu^{2+},\;Fe^{2+},\;Co^{2+},\;Ba^{2+},\;and\;Cd^{2+}$. The divalent cations $Ca^{2+},\;Mn^{2+},\;and\;Ni^{2+}$and the trivalent ion $Gd^{3+}$ did not induce currents nor did they inhibit $Mg^{2+}$ transport. The nonselective cation $La^{3+}$ abolishes $Mg^{2+}$ uptake. Computer analysis of the SLC41Al protein structure reveals that it belongs to MgtE protein family & suggested that the human solute carrier, SLC41Al, might be a eukaryotic $Mg^{2+}$ transporter closely related $(60-70\%)$ protein encoded by SLC41A2 is a $Mg^{2}+$ transporter that might be involved in magnesium homeostasis in epithelial cells also transports a range of other divalent cations: $Ba^2,\;Ni^2,\;CO^2,\;Fe^2,\;or\;Mn^2,\;but\;not\;Ca^2,\;Zn^2,\;or\;Cu^{2+}$ that may have related functional properties.

• Nuclear Medicine and Molecular Imaging
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• v.41 no.5
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• pp.343-349
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• 2007

Radioiodide transport has been extensively and successfully used in the evaluation and management of thyroid disease. The molecular characterization of the sodium/iodide symporter (NIS) and cloning of the NIS gene has led to the recent expansion of the use of radioiodide to cancers of the breast and other nonthyroidal tissues exogenously transduced with the NIS gene. More recently, discoveries regarding the functional analysis and regulatory processes of the NIS molecule are opening up exciting opportunities for new research and applications for NIS and radio iodide. The success of NIS based cancer therapy is dependent on achievement of maximal radioiodide transport sufficient to allow delivery of effective radiation doses. This in turn relies on high transcription rates of the NIS gene. However, newer discoveries indicate that nontranscriptional processes that regulate NIS trafficking to cell membrane are also critical determinants of radioiodide uptake. In this review, molecular mechanisms that underlie regulation of NIS transcription and stimuli that augment membrane trafficking and functional activation of NIS molecules will be discussed. A better understanding of how the expression and cell surface targeting of NIS proteins is controlled will hopefully aid in optimizing NIS gene based cancer treatment as well as NIS based reporter-gene imaging strategies.

• The Korean Journal of Nuclear Medicine
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• v.29 no.1
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• pp.105-109
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• 1995

L-3-[$^{123}I$]iodo-${\alpha}$-methyltyrosine([$^{123}I$] IMT) was synthesized by electrophilic radio-iodination using chloramine-T and Iodobead in phosphate buffered solution. And the biodistribution was examined in 9L glioma bearing rats. The radiosynthesis of [$^{123}I$]IMT with iodobead was simpler and higher in radiochemical yield(88%) than the method using chloramine-T(83%) as radioiodinating reagent. The highest yield was obtained from the reaction using 1 piece of Iodobead, $200{\mu}g$ ${\alpha}$-methyltyrosine in $100{\mu}l$ phosphate-buffered solution(pH 5.5) and the reaction was completed in 7min. 24 hours after the injection, the biodistribution in 9L glioma transplanted rats revealed the in vivo deiodination, the excretion via kidney, and 3 times higher uptake in the tumor than normal brain. These results suggest the promising clinical use of [$^{123}I$] IMT in the various malignancies.

• Journal of Periodontal and Implant Science
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• v.36 no.3
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• pp.783-796
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• 2006

The periodontium is a topographically complex organ consisting of epithelial tissue, soft and mineralized tissues. Structures comprising the periodontium include the gingiva, periodontal ligament (PDL) , cementum and the alveolar bone. The molecular mechanism of differentiation in PDL fibroblast cells remain unclear. Amino acid transporters play an important role in supplying nutrition to normal and cancer cells and for cell proliferation. Amino acid transport system L is a major nutrient transport system responsible for the Na+-independent transport of neutral amino acids including several essential amino acids. The system L is divided into two major subgroups, the L-type amino acid transporter 1 (LAT1) and the L-type amino acid transporter 2 (LAT2). In this study, the expression pattern of amino acid transport system L was, therefore, investigated in the differentiation of PDL fibroblast cells. To determine the expression level of amino acid transport system L participating in intracellular transport of amino acids in the differentiation of PDL fibroblast cells, it was examined by RT-PCR, observation of cell morphology, Alizaline red-S staining and uptake analysis after inducing experimental differentiation in PDL fibroblast cells isolated from mouse molar teeth. The results are as follows. 1. The LAT1 mRNA was expressed in the early stage of PDL fibroblast cell differentiation. This expression level was gradually reduced by differentiation- inducing time and it was not observed after the late stage. 2. The expression level of LAT2 mRNA was increased in time-dependent manner during differentiation induction of PDL fibroblast cells. 3. There was no changes in. the expression level of 4F2hc mRNA, the cofactor of LAT1 and LAT2, during differentiation of PDL fibroblast cells. 4. The expression level of ALP mRNA was gradually increased and the expression level of Col I mRNA was decreased during differentiation of PDL fibroblast cells. 5. The L-leucine transport was reduced by time from the early stage to the late stage in PDL fibroblast cell differentiation. As the results, it is considered that among neutral ammo acid transport system L in differentiation of PDL fibroblast cells, the LATl has a key role in cell proliferation in the early stage of cell differentiation and the LAT2 has an important role in the late stage of cell differentiation for providing cells with neutral amino acids including several essential amino acids.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.5
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• pp.397-403
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• 2015

Heavy metals reduce the photosynthetic efficiency and disrupt metabolic reactions in a concentration-dependent manner. Moreover, by replacing the metal ions in metalloproteins that use essential metal ions, such as Cu, Zn, Mn, and Fe, as co-factors, heavy metals ultimately lead to the formation of reactive oxygen species (ROS). These, in turn, cause destruction of the cell membrane through lipid peroxidation, and eventually cause the plant to necrosis. Given the aforementioned factors, this study was aimed to understand the physiological responses of rice to cadmium (Cd) and arsenic (As) toxicity and the effect of essential metal ions on homeostasis. In order to confirm the level of physiological inhibition caused by heavy metal toxicity, hydroponically grown rice (Oryza sativa L. cv. Dongjin) plants were exposed with $0-50{\mu}M$ cadmium (Cd, $CdCl_2$) and arsenic (As, $NaAsO_2$) at 3-leaf stage, and then investigated malondialdehyde (MDA) contents after 7 days of the treatment. With increasing concentrations of Cd and As, the MDA content in leaf blade and root increased with a consistent trend. At 14 days after treatment with $30{\mu}M$ Cd and As, plant height showed no significant difference between Cd and As, with an identical reduction. However, As caused a greater decline than Cd for shoot fresh weight, dry weight, and water content. The largest amounts of Cd and As were found in the roots and also observed a large amount of transport to the leaf sheath. Interestingly, in terms of Cd transfer to the shoot parts of the plant, it was only transported to upper leaf blades, and we did not detect any Cd in lower leaf blades. However, As was transferred to a greater level in lower leaf blades than in upper leaf blades. In the roots, Cd inhibited Zn absorption, while As inhibited Cu uptake. Furthermore, in the leaf sheath, while Cd and As treatments caused no change in Cu homeostasis, they had an antagonist effect on the absorption of Zn. Finally, in both upper and lower leaf blades, Cd and As toxicity was found to inhibit absorption of both Cu and Zn. Based on these results, it would be considered that heavy metal toxicity causes an increase in lipid peroxidation. This, in turn, leads to damage to the conductive tissue connecting the roots, leaf sheath, and leaf blades, which results in a reduction in water content and causes several physiological alterations. Furthermore, by disrupting homeostasis of the essential metal ions, Cu and Zn, this causes complete heavy metal toxicity.

• Korean Journal of Environmental Agriculture
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• v.41 no.4
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• pp.236-244
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• 2022

BACKGROUND: Liquid fertilizers can provide nutrients to crops effectively and quickly. Amino acid liquid fertilizers produced by decomposing the residues of rendered livestock carcasses are expected to be effective in improving the productivity and quality of crops. METHODS AND RESULTS: The treatment conditions for maize cultivation were control (Cn), inorganic fertilizer (IF), inorganic fertilizer and rendering residue liquid fertilizer (IF+RALF), compost (CP), compost and rendering residue liquid fertilizer (CP+RALF). Crop productivity, sugar content, and nutrient uptake were investigated after maize harvest in the field applied with liquid fertilizers. Maize yields ranged from 87.6-158 g/plant, and the yield increased by 7.9% and 12.9% in IF+RALF IF+RALF and CP+RALF than in IF and CP, respectively. The maize sugar content increased in the range of 0.1-0.5 brix % by rendering residue liquid fertilizer (RALF) fertilization, and the sugar content was the highest in CP+RALF. There was no significant change in soil chemical properties of the soil due to liquid fertilizer treatment. CONCLUSION(S): RALF increased yield and sugar content in maize cultivation, and fertilization with organic fertilizers was more effective for maize cultivation than inorganic fertilizers. Residues of rendered livestock carcass can be recycled as amino acid fertilizers, which can be effectively used for crop production and quality improvement.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.332-337
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• 2013

BACKGROUND: Newly reclaimed land has poor soil environment for crop growth since it is high in salt concentration but low in organic content compared with ordinary soil. It is known that whole-crop-barley can grow better in the soil of relatively high salt concentration than other crops but, the growth is poor at the concentration if higher than certain amount and it is a difficulty to secure productivity. Hence, the level of soil salt concentration suitable for the production of bulky feed in newly reclaimed land has been investigated. METHODS AND RESULTS: At Saemanguem reclaimed land, the land for the soil salt concentration electrical conductivity (EC) 0.8, 3.1, 6.5, 11.0 dS/m was selected; and chemical fertilizer $N-P_2O_5-K_2O$ (150-100-100kg/ha) was tested; and forage barley 220kg/ha were sown. The soil salt concentration during the cultivation period decreased in the order of harvest season>earing season>sowing season>wintering season, and the salt concentration in harvest season is 1.4-4.2 times higher than that of the sowing season. The higher the salt concentration, the poorer the over ground growth due to poor rooting; especially at EC 11.0 ds/m there was emergence but, it blighted after wintering. The Yield from the soil salt concentration 3.1dS/m and 6.5 dS/m was 68% and 35% from that of the soil salt concentration 0.8 dS/m (8.8 MT/ha) respectively. The proline content in early life stage was more than that of the harvest season, and it increased with salt concentration. The higher salt concentration, the more $Na_2O$ and MgO content in harvest season; but the higher the salt concentration, the less the content of N, $P_2O_5$, $K_2O$ and CaO. CONCLUSION(S): When the soil salt concentration becomes higher than 3.1 dS/m, the yield becomes poor because there is serious growth inhibition of forage barley both in root part and above aerial part that results in unbalanced absorption of nutrients. Therefore, it is recommended that the salt concentration should be lowered below 3.1 dS/m by underground drainage facilities or irrigating water for the stable production of whole-crop-barley.

• The Korean Journal of Nuclear Medicine
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• v.33 no.3
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• pp.289-297
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• 1999

Purpose: Misonidazole is a radiosensitizer that binds in hypoxic cells. The purpose of this study was to find out the feasibility of I-131-Iodomisonidazole (IMISO) for imaging of tumor hypoxia. Materials and Methods: Tosyl precursor was dissolved in acetonitrile and I-131-NaI was added to synthesize IMISO. Balb/c mice inoculated with CT-26 adenocarcinoma were injected with IMISO. Mice were sacrificed at 1, 2, 4, 24 hr and % of injected dose per gram of tissue (%ID/g) was determined. For scintigraphy and MRI, mouse bearing CT-26 adenocarcinoma was administered with IMISO and imaging was performed 4 hr after. Then, mouse body was fixed and microtomized slice was placed on radiographic film for autoradiography Results: %ID/g of tumor was 1.64 (1h), 0.98 (2h), 0.85 (4h) and 0.20 (24h), respectively. At 24h, %ID/g of tumor was higher than that of all other tissues except thyroid. Tumor to muscle ratio increased with time and tumor to blood ratio also increased with time and reached 1.53 at 24 hr. On autoradiogram, tumor was well visualized as an increased activity in central hypoxic area of the tumor which corresponds to the area of high signal intensity on T2-weighted MR image. On scintigraphy, tumor uptake was visualized. Conclusion: This results suggest that IMISO may have a potential for tumor hypoxia imaging in mouse model. However, further study is needed to improve it's localization in tumor tissue and to achieve acceptable images of tumor hypoxia.