• Childhood Kidney Diseases
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• 제26권2호
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• pp.107-110
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• 2022

Nephrogenic diabetes insipidus, decreased ability to concentrate urine, with production of large amounts of urine, is caused by the refractory response of renal tubules to the action of antidiuretic hormone. This rare disorder, known as X-linked nephrogenic diabetes insipidus, is caused by a mutation in the AVPR2 gene. Because it is hereditary, most patients are male. This report highlights a case of nephrogenic diabetes insipidus in a 3-year 5-month-old female; upon presentation to the hospital, her symptoms included frequent urinationand consumptionof a significant amount ofwater,which had begun2 years ago. The results of blood tests showed increased levels of serum antidiuretic hormone, and sellar magnetic resonance imaging showed no abnormality. The results of the water restriction test and the desmopressin administration test confirmed the diagnosis of nephrogenic diabetes insipidus showing a partial response to desmopressin. The results of genetic testing indicated the presence of an AVPR2 mutation, a heterozygous missense mutation (p.Val88Met), suggesting inheritance of X-linked nephrogenic diabetes insipidus. This report describes a significant case of symptomaticX-linked nephrogenic diabetes insipidus in a female patient who showed a partial response to desmopressin.

• 한국환경농학회지
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• 제28권3호
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• pp.243-248
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• 2009

환경조건이 불량한 고랭지 경사지 밭에서 정식 초기 산채 (곰취, 참취)류의 생육 및 수량과양분함량의 관계를 검토하고자 시험을 실시하였다. 시험 후 토양 화학성 변화는 참취, 곰취 시험포 모두 비료 시용량이 증가함에 따라 다소 높아졌고, 토양유기물 함량은 요소 처리보다 부산물퇴비 시용구가 높은 것으로 나타났다. 수확기 참취 및 곰취의 총 질소 함량은 질소시비량이 증가 할수록 높아졌으나 부산물퇴비 처리구에서는 경향성이 없는 것으로 나타났다. 참취 및 곰취 모두 질소시비량이 많을수록 생육이 양호한 것으로 나타났으며, 참취 생육의 경우 질소 120 kg/ha 처리구와 160 kg/ha 처리구에서 가장 높은 생육을 나타났다. 참취의 수량도 질소 처리 수준이 증가할수록 수량은 증가하여 질소 120 kg/ha 처리구와 160 kg/ha 처리구에서 높게 나타났으며, 160kg/ha 처리구에서는 다소 감소하였으나 통계적으로는 유의성이 없는 것으로 나타났다.

• 박물관보존과학
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• 제17권
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• pp.55-68
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• 2016

국립중앙박물관은 일제강점기 조선총독부박물관에서 발굴되었던 석암리 9호분 출토유물의 보존처리 및 연구를 진행하고 있다. 본 연구에서는 출토된 유물 가운데 청동 및 금동단추의 과학적 조사를 실시하여 제작방법을 유추하였다. 관찰 결과 청동 및 금동 단추는 반구형으로 외형적 특징이 비슷하나 걸이의 단면 형태 및 크기에서 차이점이 보인다. 걸이의 단면형태는 원형과 사각형이며 두 그룹으로 분류되고 이 그룹 내에서 길이를 기준으로 세분화를 하였다. 원형 걸이의 경우, 걸이의 형태 및 설치 위치가 다양하며 두께가 일정하지 않으며 몸체와 걸이의 경계부가 고르지 못하다. 사각형 걸이는 걸이의 형태 및 위치가 일정하며 몸체의 바닥면에 걸이가 연결되었으며 표시선으로 보이는 홈이 존재한다. 또한 X-ray 조사결과, 원형 걸이의 경계부분은 두꺼우며 이는 흙이나 이물질이 아닌 금속물질로 확인된다. 제작기법은 밀랍법으로 추측되며 걸이의 단면형태에 따라 몸체와 접합과정이 차이가 있다.

• Journal of Ginseng Research
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• 제37권4호
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• pp.413-424
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• 2013

Korean Red Ginseng extract (KRGE) is a traditional herbal medicine utilized to prevent endothelium dysfunction in the cardiovascular system; however, its underlying mechanism has not been clearly elucidated. We here examined the pharmacological effect and molecular mechanism of KRGE on apoptosis of human umbilical vein endothelial cells (HUVECs) in a serum-deprived apoptosis model. KRGE protected HUVECs from serum-deprived apoptosis by inhibiting mitochondrial cytochrome c release and caspase-9/-3 activation. This protective effect was significantly higher than that of American ginseng extract. KRGE treatment increased antiapoptotic Bcl-2 and Bcl-$X_L$ protein expression and Akt-dependent Bad phosphorylation. Moreover, KRGE prevented serum deprivation-induced subcellular redistribution of these proteins between the mitochondrion and the cytosol, resulting in suppression of mitochondrial cytochrome c release. In addition, KRGE increased nitric oxide (NO) production via Akt-dependent activation of endothelial NO synthase (eNOS), as well as inhibited caspase-9/-3 activities. These increases were reversed by co-treatment of cells with inhibitors of eNOS and phosphoinositide 3-kinase (PI3K) and pre-incubation of cell lysates in dithiothreitol, indicating KRGE induces NO-mediated caspase modification. Indeed, KRGE inhibited caspase-3 activity via S-nitrosylation. These findings suggest that KRGE prevents serum deprivation-induced HUVEC apoptosis via increased Bcl-2 and Bcl-$X_L$ protein expression, PI3K/Akt-dependent Bad phosphorylation, and eNOS/NO-mediated S-nitrosylation of caspases. The cytoprotective property of KRGE may be valuable for developing new pharmaceutical means that limit endothelial cell death induced during the pathogenesis of vascular diseases.

• Asian-Australasian Journal of Animal Sciences
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• 제8권1호
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• pp.67-73
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• 1995

This study showed that models of energy utilization(EU) developed for grazing cattle in oil palm plantations is valid as the simulated results shows an agreement with actual data of calves and cows body weight changes collected from Brahman x Kedah-Kelantan herd on Pengeli Timor Plantation. Simulation runs on EU models demonstrated that the growth pattern of male and female calves and the weight changes of cows are similar and showed slight variation from the actual data but with no significant difference (p > 0.05). Parameter values such as metabolizability (q), dry matter digestibility(DMD) of herbage and voluntary intake of grazing cattle (VIG) and faecal output/body weight ratio (F) of the animals which were collected from the field are essential in bearing the pattern of body weight changes of the calves and cows in relation to increase in time, physiological status and quality of herbage grazed by these animals in the production system. The EU models is suitable for determining the metabolizable energy requirements and to predict the production of grazing cattle according to quality of the feed on offer.

• 한국양식학회지
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• 제20권3호
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• pp.194-198
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• 2007

Reproductive traits of Palaemon gravieri such as embryo size, number of embryo (fecundity), incubation period, larval development mode, larval development period, larval survival and larval growth were described and compared to analyze the correlation among those traits. Embryo volume is a primary factor determining other ensuing reproductive features. Egg volume was $0.042mm^3$ in the first developmental stage. Embryo volume in P. gravieri was comparatively small which is indicative of great number of embryo (y = 3.0161x + 0.0185 $R^2$ = 0.74 positive isometric relationship) and relatively long incubation period. Larvae survived from zoea 1 to post-larvae and it took 45 days at $22^{\circ}C$. Survival rate of the larvae was rather great in the early stage and thereafter steadily decreased. Daily growth rate of larvae in P. gravieri at $22^{\circ}C$ was 0.0195 mm on average. They grew steadily as time went by. Incubation period was between 10-14 days at $22^{\circ}C$. Larval development mode was almost complete planktotrophic. PNR (point of no return) appeared to be the third day on average. Survival rate of larvae without feeding declined rapidly between 3 and 4 days. Larval development period and stage frequency were 23-30 days and 11 stages which imply prolonged larval period and high mortality. The pattern of brood production followed fast successive parturial pattern. Most ovigerous female had mature ovary when they performed parturial molt soon after hatching (larval release).

• 한국주조공학회지
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• 제17권3호
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• pp.286-291
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• 1997

Influence of Si contents on the mechanical properties and microstructure of austempered ductile iron was investigated. Four different Si contents between 2.0 and 2.9% were used. Austenitizing was performed at $890^{\circ}C$ for 2 hrs and austempering temperatures were both 340 and $380^{\circ}C$ for 0.5, 1, and 2 hrs. Nodule content was more than $300/mm^2$ and nodularity was more than 90%. Microstructure was revealed using nital and retained austenite was measured by x-ray diffractometer. Tensile test, no-notch Charpy impact test and wear test were performed. Tensile strength was improved as Si content increased and both elongation and impact toughness had peak at 2.6%Si. The specimen austempered at $380^{\circ}C$ showed lower tensile strength than that of $340^{\circ}C$, but showed higher elongation. However, austempering temperature of $380^{\circ}C$ was desirable because that of $340^{\circ}C$ was close to lower bainite transformation. As austempering time increased, tensile strength and elongation were improved and optimum condition was obtained for 2 hrs heat treatment.

• 혜화의학회지
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• 제24권2호
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• pp.35-57
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• 2016

Objectives : The purpose of this study is to prove the effect and mechanism of Gamikyejakjimogawusul-tang(GKHA) herbal acupuncture on induced rheumatoid arthritis model of DBA/1 mice. Methods : We check effect of GKHA extract on the AST, ALT, Creatinine, BUN of serum and cell viability of GK extract in RAW 264.7 cells to test the stability of this study. In vitro, we measure total phenol contents, total flavonoid contents, DPPH free radical scavenging activity, ABTS cation radical scavenging activity of Gamikyejakjimogawusul-tang, effect of GK extract on ROS(Reactive Ooxygen Species) production to estimate a anti-oxidant capacity, and we also measure effect of GK extract on NO (Nitric Oxid), IL-$1{\beta}$, IL-6, IL-17, IL-21, TNF-${\alpha}$, MCP-1, GM-CSF production in RAW 264.7 cells to estimate a anti-inflammatory efficacy. In vivo, we compare a rheumatoid arthritis manifestation between control and experimental group and estimate a AI. Then we check effect of GKHA on the level of WBC, neutrophil, lympocyte, monocyte in the blood to see the effect of immune cells in blood. In addition we measure effect of GKHA on the level of hs-CRP, IgM, IgG, IL-$1{\beta}$, IL-6, IL-17, IL-21, TNF-${\alpha}$, MCP-1, GM-CSF in serum. We observe effects of GKHA on imaging of cartilage degeneration using micro CT-arthrography in paw hind. And we calculate effects of GKHA that reduced BV ratio, BS/BV ratio using 3D Micro-CT. Lastly we observe effects of GKHA histopathologic examination analysis. Results : 1. The toxicity on liver and kidney was disregardable and the cytotoxicity against RAW 264.7 cells was also disregardable. 1. Total phenol contents and total flavonoid contents in GK extract were in high level. 2. DPPH free radical scavenging activity and ABTS cation radical scavenging activity were increased according to concentration of GK extract 3. ROS production was significantly decreased in GK extract (at 10, $100{\mu}g/ml$). 4. NO, IL-6, TNF-${\alpha}$, MCP-1 production were significantly decreased in GK extract(at 10, $100{\mu}g/ml$). IL-17, GM-CSF production were significantly decreased in GK extract(at 1, 10, $100{\mu}g/ml$). IL-$1{\beta}$, IL-21 production were also decreased but there was no statistical significance. 5. 25x observation after H&E and M-T staining, infiltration of immune cells and subsidence of the cartilage and damage to the synovial cells were decreased. Conclusions : This study showed that GKHA extract had anti-oxidant capacity, anti-inflammatory efficacy. GKHA extract also had inhibiting effect on the process of rheumatoid arthritis and can protect joint and cartilage. So we expect that GKHA extract can be a meaningful treatment to rheumatoid arthritis patients.

• Journal of Korean Society for Atmospheric Environment
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• 제10권E호
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• pp.311-324
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• 1994

The quantitative knowledge of N $O_{\gamma}$ (＝N $O_{x}$ ＋HN $O_3$＋/PAN＋N $O_3$ ＋$N_2$ $O_{5}$ ＋HN $O_2$＋N $O_3$$^{-10}$ ＋organic nitrates＋......)distribution is essential in tropospheric chemistry, especially, especially that related to understanding the processes leading to ozone production. Ambient concentrations of NO, N $O_2$, HN $O_3$ and PAN as well as total N $O_{\gamma}$ were measured during June and early July 1992 at a rural site(Candor, NC), in the central Piedmont region of NC. The measurements of N $O_{\gamma}$ species were made in an effort to provide a comprehensive understanding of nitrogen chemistry and to investigate the total nitrogen budget at the site. N $O_{\gamma}$, N $O_2$, and NO showed diurnal variations with maxima in the morning. The maximum N $O_{\gamma}$ concentration reached was 14.5 ppbv, and the maximum concentrations of NO and N $O_2$ were 5.4 and 7.8 PPbv, respectively. The mean N $O_{\gamma}$ concentration as found to be 2.88$\pm$1.58 ppbv(n=743). The mean concentrations of NO and N $O_2$, were found to be 0.15 $\pm$ 0.29 ppbv(n＝785) and 1.31 $\pm$ 0.99 ppbv(n＝769). Products of photochemical oxidants, (N <$O_{\gamma}$-N $O_{x}$ ), such as HN $O_3$ and PAN, as well as ozone showed diurnal variation with maxima in the afternoon and minima at night The fractions of individual reactive nitrogen species to total N $O_{\gamma}$ were investigated and contrasted to the results from remote marine site and rural continental sites. N $O_{x}$ was the major species to total N $O_{\gamma}$(45%). NO concentrations appeared to be nearly constant whether the Prevailing winds were from continental areas or from oceanic areas. Linear regression of $O_3$ with (N $O_{\gamma}$- N $O_{x}$ )/N $O_{\gamma}$ (i.e. percent N $O_{x}$ converted to the photochemical products of N $O_{\gamma}$) yielded ( $O_3$) ＝25.8 〔 N $O_{\gamma}$-N $O_{x}$ 〕/(N $O_{\gamma}$) ＋27, ( $r^{2}$＝0.58). The regression intercept is interpreted as the ozone back ground (intercept=27ppbv) and the slope suggests that 8.6 molecules of ozone are formed per molecule of N $O_{x}$ oxidized products (when the average N $O_{\gamma}$ concentration, about 3 ppbv at the site, is used). The N $O_{x}$ N $O_{\gamma}$ ratio was used as an indicator of the chemical age of airmasses and the ratio showed strong positive correlations with HN $O_3$( $r^{2}$=0.58), PAN ( $r^{2}$=0.46) and $O_3$( $r^{2}$=0.62). Larger N $O_{\gamma}$ and N $O_{x}$ N $O_{\gamma}$ ratio were found when winds came from continental sides. It may suggest that synoptic meteorological conditions and transport of N $O_{x}$ are important in the distribution of N $O_{\gamma}$ and its relationship with photochemical oxidants at the site.

• Asian-Australasian Journal of Animal Sciences
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• 제16권11호
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• pp.1555-1559
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• 2003

The objective of the present study was to explore associations between five microsatellites linked to $Fec^B$ and $FecX^I$ genes and litter size in Small Tail Han sheep. The polymorphisms of five microsatellite loci, OarAE101, BM1329, BMS2508, TGLA54 and TGLA68 were detected in 244 ewes of Small Tail Han sheep. Analysis of association between three microsatellite loci (BMS2508, BM1329 and OarAE101) located in the 10 cM region covering the $Fec^B$ gene (Booroola gene) and litter size in Small Tail Han sheep indicated that BMS2508 had significant effect on litter size in the second parity (p<0.05), but no significant effect on litter size in the first parity (p>0.05), while the other two microsatellite loci had no significant effect on litter size in both the first and the second parity in Small Tail Han sheep (p>0.05). At microsatellite locus BMS2508, least squares means in the second parity of genotypes 101/111 and 99/109 were significantly higher than those of genotypes 99/99, 99/101, 99/111 and 99/115 (p<0.05); least squares mean in the second parity of genotype 101/111 was significantly higher than that of genotypes 109/111 and 111/111 (p<0.05). Results of this study also indicated that two microsatellite loci (TGLA54 and TGLA68) that confined the 28.7 cM region covering the $FecX^I$ gene (Inverdale gene) did not affect litter size in both the first and the second parity in Small Tail Han sheep significantly (p>0.05). The information found in the present study is very important for improving the reproductive performance in sheep breeds by marker assisted selection.